LOCUS CVU73899 4788 bp DNA linear SYN 11-APR-1997
DEFINITION Cloning vector pJB3, complete sequence.
ACCESSION U73899
VERSION U73899.1
KEYWORDS .
SOURCE Cloning vector pJB3
ORGANISM Cloning vector pJB3
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 4788)
AUTHORS Blatny,J.M., Brautaset,T., Winther-Larsen,H.C., Haugan,K. and
Valla,S.
TITLE Construction and use of a versatile set of broad-host-range cloning
and expression vectors based on the RK2 replicon
JOURNAL Appl. Environ. Microbiol. 63 (2), 370-379 (1997)
PUBMED 9023917
REFERENCE 2 (bases 1 to 4788)
AUTHORS Blatny,J.M.
TITLE Direct Submission
JOURNAL Submitted (11-OCT-1996) UNIGEN Center for Molecular Biology and
Laboratory of Biotechnology, Norwegian University of Science and
Technology, 7005 Trondheim 7005, Norway
FEATURES Location/Qualifiers
source 1..4788
/organism="Cloning vector pJB3"
/mol_type="genomic DNA"
/db_xref="taxon:53569"
/note="based on the minimal replicon of RK2; sequence was
established by combining previously reported sequences of
the genes in the vector, and by sequencing important
junction sites"
misc_feature 181..291
/note="origin of transfer from RK2"
gene 571..1431
/gene="bla"
CDS 571..1431
/gene="bla"
/function="ampicillin resistance"
/note="originates from pBR322"
/codon_start=1
/transl_table=11
/product="beta-lactamase"
/protein_id="AAB51669.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
misc_feature 1677..2202
/note="origin of vegetative replication from RK2"
regulatory 2430..2548
/regulatory_class="promoter"
/note="promoter of the neomycin phosphotransferase gene"
gene 2600..3748
/gene="trfA"
CDS 2600..3748
/gene="trfA"
/function="binds to oriV"
/function="initiator protein necessary for replication"
/codon_start=1
/transl_table=11
/product="TrfA"
/protein_id="AAB51670.1"
/translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVG
SMVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKK
RKTAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALF
TTRNKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPI
TFTFYELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLD
RGKKTSRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKL
ETFRLMCGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
CDS complement(<4220..>4451)
/codon_start=3
/transl_table=11
/product="beta-galactosidase"
/protein_id="AAB51671.1"
/translation="AMTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQL
NRLAAHPPFASWRNSEEARTDRPSQQLRSLNG"
BASE COUNT 1171 a 1236 c 1357 g 1024 t
ORIGIN
1 agatctgctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt
61 ttcccagtca cgacgttgta aaacgacggc cagtgaatta attcttgaag acgaaagggc
121 ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagagctta
181 cggccagcct cgcagagcag gattcccgtt gagcaccgcc aggtgcgaat aagggacagt
241 gaagaaggaa cacccgctcg cgggtgggcc tacttcacct atcctgcccg gctgacgccg
301 ttggatacac caaggaaagt ctacacgaac cctttggcaa aatcctgtat atcgtgcgaa
361 aaaggatgga tataccgaaa aaatcgctat aatgaccccg aagcagggtt atgcagcgga
421 aaagatccgt cgatcgaccc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
481 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
541 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
601 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
661 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
721 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
781 aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt
841 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
901 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
961 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
1021 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
1081 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
1141 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
1201 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
1261 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
1321 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
1381 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
1441 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
1501 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
1561 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
1621 cgggggatca ggaccgctgc cggagcgcaa cccactcact acagcagagc catgtagggc
1681 cgccggcgtt gtggatacca cgcggaaaac ttggccctca ctgacagatg aggggcggac
1741 gttgacactt gaggggccga ctcacccggc gcggcgttga cagatgaggg gcaggctcga
1801 tttcggccgg cgacgtggag ctggccagcc tcgcaaatcg gcgaaaacgc ctgattttac
1861 gcgagtttcc cacagatgat gtggacaagc ctggggataa gtgccctgcg gtattgacac
1921 ttgaggggcg cgactactga cagatgaggg gcgcgatcct tgacacttga ggggcagagt
1981 gatgacagat gaggggcgca cctattgaca tttgaggggc tgtccacagg cagaaaatcc
2041 agcatttgca agggtttccg cccgtttttc ggccaccgct aacctgtctt ttaacctgct
2101 tttaaaccaa tatttataaa ccttgttttt aaccagggct gcgccctggc gcgtgaccgc
2161 gcacgccgaa ggggggtgcc cccccttctc gaaccctccc ggcccgctaa cgcggcaccc
2221 catcccccca ggggctgcgc ccctcggccg cgaacgacct caccccaaaa atggcagcca
2281 cgtagaaagc cagtccgcag aaacggtgct gaccccggat gaatgtcagc tactgggcta
2341 tctggacaag ggaaaacgca agcgcaaaga gaaagcaggt agcttgcagt gggcttacat
2401 ggcgatagct agactgggcg gttttatgga cagcaagcga accggaattg ccagctgggg
2461 cgccctctgg taaggttggg aagccctgca aagtaaactg gatggctttc ttgccgccaa
2521 ggatctgatg gcgcagggga tcaagatcga cggatcgatc cggggaatta attccggggc
2581 aatcccgcaa ggagggtgaa tgaatcggac gtttgaccgg aaggcataca ggcaagaact
2641 gatcgacgcg gggttttccg ccgaggatgc cgaaaccatc gcaagccgca ccgtcatgcg
2701 tgcgccccgc gaaaccttcc agtccgtcgg ctcgatggtc cagcaagcta cggccaagat
2761 cgagcgcgac agcgtgcaac tggctccccc tgccctgccc gcgccatcgg ccgccgtgga
2821 gcgttcgcgt cgtctcgaac aggaggcggc aggtttggcg aagtcgatga ccatcgacac
2881 gcgaggaact atgacgacca agaagcgaaa aaccgccggc gaggacctgg caaaacaggt
2941 cagcgaggcc aagcaggccg cgttgctgaa acacacgaag cagcagatca aggaaatgca
3001 gctttccttg ttcgatattg cgccgtggcc ggacacgatg cgagcgatgc caaacgacac
3061 ggcccgctct gccctgttca ccacgcgcaa caagaaaatc ccgcgcgagg cgctgcaaaa
3121 caaggtcatt ttccacgtca acaaggacgt gaagatcacc tacaccggcg tcgagctgcg
3181 ggccgacgat gacgaactgg tgtggcagca ggtgttggag tacgcgaagc gcacccctat
3241 cggcgagccg atcaccttca cgttctacga gctttgccag gacctgggct ggtcgatcaa
3301 tggccggtat tacacgaagg ccgaggaatg cctgtcgcgc ctacaggcga cggcgatggg
3361 cttcacgtcc gaccgcgttg ggcacctgga atcggtgtcg ctgctgcacc gcttccgcgt
3421 cctggaccgt ggcaagaaaa cgtcccgttg ccaggtcctg atcgacgagg aaatcgtcgt
3481 gctgtttgct ggcgaccact acacgaaatt catatgggag aagtaccgca agctgtcgcc
3541 gacggcccga cggatgttcg actatttcag ctcgcaccgg gagccgtacc cgctcaagct
3601 ggaaaccttc cgcctcatgt gcggatcgga ttccacccgc gtgaagaagt ggcgcgagca
3661 ggtcggcgaa gcctgcgaag agttgcgagg cagcggcctg gtggaacacg cctgggtcaa
3721 tgatgacctg gtgcattgca aacgctaggg ccttgtgggg tcagttccgg ctgggggttc
3781 agcacctgca tgattattga agcatttatc agggttattg tctcatgagc ggatacatat
3841 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
3901 cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca
3961 cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc
4021 tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg
4081 gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga
4141 ttgtactgag agtgcaccat atatgcggtg tgaaataccg cacagatgcg taaggagaaa
4201 ataccgcatc aggcgccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt
4261 gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag
4321 ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgaattcga
4381 gctcggtacc cggggatcct ctagagtcga cctgcaggca tgcaagcttg gcgtaatcat
4441 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
4501 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
4561 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
4621 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
4681 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
4741 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgc
//