LOCUS CSU60580 2932 bp DNA linear BCT 19-OCT-2010
DEFINITION Clostridium stercorarium cellodextrin-phosphorylase (cepB) gene,
complete cds.
ACCESSION U60580
VERSION U60580.1
KEYWORDS .
SOURCE Thermoclostridium stercorarium
ORGANISM Thermoclostridium stercorarium
Bacteria; Firmicutes; Clostridia; Eubacteriales; Oscillospiraceae;
Thermoclostridium.
REFERENCE 1 (bases 1 to 2932)
AUTHORS Reichenbecher,M., Lottspeich,F. and Bronnenmeier,K.
TITLE Purification and properties of a cellobiose phosphorylase (CepA)
and a cellodextrin phosphorylase (CepB) from the cellulolytic
thermophile Clostridium stercorarium
JOURNAL Eur. J. Biochem. 247 (1), 262-267 (1997)
PUBMED 9249035
REFERENCE 2 (bases 1 to 2932)
AUTHORS Reichenbecher,M., Bronnenmeier,K., Lottspeich,F. and
Staudenbauer,W.L.
TITLE Direct Submission
JOURNAL Submitted (11-JUN-1996) Technical University of Muenchen, Institute
for Microbiology, Arcisstr. 16, Muenchen, Bavaria D-80290, Germany
FEATURES Location/Qualifiers
source 1..2932
/organism="Thermoclostridium stercorarium"
/mol_type="genomic DNA"
/strain="NCIB 11754"
/type_material="type strain of Thermoclostridium
stercorarium"
/db_xref="taxon:1510"
gene 1..2343
/gene="cepB"
CDS 1..2343
/gene="cepB"
/EC_number="2.5.1.49"
/function="phosphorolysis of beta-1,4 linkages of
cellotriose, cellotetraose, cellopentaose and
cellohexaose"
/note="similar to Genbank Accession Number U56424, U24658
and SwissProt Accession Number P20471"
/codon_start=1
/transl_table=11
/product="cellodextrin-phosphorylase"
/protein_id="AAC45511.1"
/translation="MRYGYFDEKAREYVITRPDTPTPWINYIGNGKYGGIVTNTGGGY
SFHKDPQNRRITRYRYNNLPTDRPGRYIYVRDRLTGEYWNPGYQPVQRKLDSYRCRHG
MGYTVLEGEYKGIAADVTYFVPDDRDFEIWLVQIRNLCHVERNLQVFSYAEFCFWDAI
MDQQNVDWVQQINQGRYEDRLITWHPHHFKDACAFFATNAEINSFDTNLEAFIGRYRC
ESNPIAVETGACSNSVSYRMNGVGAFCIDVNLKPGEEREIIFILGFTENKSTIRDEIR
DYLNVEYAKEALKRLKDSWEEYLDKLQIETPDRETNLFVNTWNQYQCKITFNWSRFVS
MYSWGLGRGIGIRDSAQDTLGVMHSIPELAGGLIKRLIHCQYTDGRVYHLFFPLTGEG
GIGDAPVVKFDWYSDDHLWLPIAANAYLKETANFDFFQSVVPYNDNKTEGTVWEHLNR
AMEFTYNHRGPHALPYSRADWNDTLNLDMGNGIAETLFTSMLFSEPPLKRFRCRISDK
RIATKYRYWYDEMKQAINEWCWDGEWYIRAFDDEGNVLGSGKNRYGKIFINSQSWAVL
SMVAPEEYAKKCLESVYRHLNTKYGIVKVYPAYPEYNPKIGGMTTYPPGAKENGGIFA
HTNPWVMIAECMMGNGRRAYQYYRQILPLTRNDDADLLEVEPYVYCQNILGKEHPQFG
IGRNSWLTGTAAWNMVAVSQYILGIRPEYDGLTVDPCIPPDWKGFKVRRIFRGCVYNI
EVRNPEGVRRCEKNCRRGVETDKIPVKPAGTVCECVVIMG"
BASE COUNT 887 a 607 c 742 g 696 t
ORIGIN
1 atgcgttacg gttattttga tgaaaaagcc cgcgaatatg ttattacaag acccgatact
61 ccgacaccat ggataaacta cataggcaac gggaaatacg gaggcatagt tacaaacacc
121 ggaggcggct acagttttca caaggaccca cagaacagaa gaattacaag atacagatac
181 aacaatctcc ccaccgacag gccgggcagg tatatttacg tccgcgacag gctgacgggc
241 gaatactgga atcccggcta tcagcccgtg cagagaaaac tcgactctta caggtgcagg
301 catggaatgg gttatactgt tctcgaaggg gagtacaagg gcattgcagc cgatgtgacc
361 tattttgtac ccgatgacag ggactttgaa atctggcttg tacaaatcag aaacctctgc
421 catgttgaaa gaaaccttca ggtcttcagt tacgccgaat tctgtttctg ggatgccata
481 atggatcagc agaatgtgga ctgggttcag cagataaacc agggaaggta tgaagacagg
541 cttataacgt ggcatccgca tcattttaag gacgcatgcg cattctttgc cacaaatgcc
601 gaaataaaca gttttgatac caatcttgag gcgtttatcg gaagataccg ctgcgaatct
661 aaccccatcg ccgtggaaac aggggcatgt tccaattccg tgtcttacag aatgaacggt
721 gtcggtgcgt tttgcatcga cgtaaattta aaaccggggg aagagcggga aattatattt
781 attttgggct ttaccgaaaa caagagtaca ataagggacg aaatccgcga ttaccttaac
841 gtggaatatg caaaagaagc tctaaaaagg ctaaaggact cctgggagga atatctggac
901 aaactgcaga ttgaaacccc cgatcgggaa accaatctct ttgttaacac atggaatcag
961 tatcagtgca aaataacctt caactggtca agatttgtat cgatgtacag ctgggggctg
1021 ggaaggggaa tagggatcag ggacagcgcc caggacacgc tcggcgttat gcattcaata
1081 cccgagctgg caggcgggct tattaaacgg ttaattcact gccagtatac cgacgggcgt
1141 gtttatcatc tgtttttccc tttgacaggc gaaggcggga tcggtgacgc cccagttgtg
1201 aaatttgact ggtattccga tgatcatctg tggctgccca tcgccgcaaa cgcatatctg
1261 aaggaaacgg cgaatttcga ttttttccaa agtgttgtgc cgtataacga caataaaacc
1321 gaaggtaccg tatgggagca tctcaacagg gccatggaat tcacatacaa ccaccgcgga
1381 ccgcacgcct tgccttatag cagagccgac tggaacgata ccctgaacct ggatatggga
1441 aacggaatag ccgaaactct ttttacttca atgcttttct cagagccgcc cttgaaacga
1501 ttcagatgcc gaatatccga caaaaggatt gcgacaaaat accggtattg gtatgatgaa
1561 atgaaacaag ccataaatga atggtgctgg gacggagaat ggtacataag ggcatttgac
1621 gatgagggta atgtccttgg ttcgggtaaa aacagatacg ggaaaatttt cattaacagc
1681 cagtcatggg cggttcttag catggttgcg cccgaagaat acgctaagaa atgcctcgag
1741 tcggtttatc ggcacctgaa cacgaagtac gggatagtta aagtatatcc ggcatatccg
1801 gaatataatc cgaaaatagg gggcatgacc acatacccgc cgggggcgaa ggaaaacggc
1861 ggtatttttg cccataccaa cccatgggta atgattgccg aatgtatgat gggcaacgga
1921 aggcgggctt atcagtacta caggcagata ctcccgttaa cgcgcaatga cgacgccgat
1981 ttgcttgaag tggaacctta cgtgtactgc cagaatatac tcggaaaaga gcatccgcag
2041 ttcggaatag gccgtaattc gtggcttaca ggcactgcag catggaatat ggttgcggta
2101 agccagtata ttttgggaat aagaccggaa tacgacggcc tgacggtgga tccgtgcatt
2161 ccgcctgact ggaaaggttt taaagtgaga agaattttcc ggggttgtgt ttacaatatc
2221 gaagtaagaa atcctgaagg cgtacggagg tgtgaaaaaa attgtcgtag aggagttgaa
2281 accgacaaaa tacccgtaaa acctgcagga acggtttgtg aatgtgttgt tataatggga
2341 tgactgtaaa aaagtaaaaa tatttaaaca tataagttca tcagcgggaa ctttttacca
2401 gtatatgcgt ctaatcaaac gtaactggca acccgctgca aaacaaatta aaaaagcata
2461 gttcacaact tcaggttgcg gctatgcttt ttttcactga aaatttcact gaaaaactta
2521 taccgtcatt acgggaataa ggaggggtta tatgaagaaa aatgctttac ttgttttaat
2581 gttcattttg ataatttttt ccggttgtac gggtggcgga aacgtcaccg atacaacgct
2641 gaaacccgag tcaggggaaa cggcaggcac taccgttgat gaaaagagcc agccctcagc
2701 cagagtttcc gacgcaaata aaattttttc ctgggaactg ttcaaagcgc ttaacgcgga
2761 ggaatcaaaa caggaaactt ttatctctcc gttttcggtt tcggcagtgc ttatgatggc
2821 ctataacggc gcagagggaa gcacgcggga agcaatggcc aaagcgatgc attatggtgg
2881 catgtccgtt gatgaattaa acagtgaata caggaatttg ttaaacagac tg
//