LOCUS CVU47119 4006 bp DNA circular SYN 10-MAY-2004
DEFINITION Cloning vector pCI, mammalian expression vector, complete sequence.
ACCESSION U47119
VERSION U47119.2
KEYWORDS .
SOURCE Cloning vector pCI
ORGANISM Cloning vector pCI
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 4006)
AUTHORS Brondyk,W.H. and Groskreutz,D.J.
TITLE pCI and pSI Mammalian Expression Vectors
JOURNAL Promega Notes 49, 7-11 (1994)
REFERENCE 2 (bases 1 to 4006)
AUTHORS Brondyk,W.H. and Groskreutz,D.J.
TITLE Direct Submission
JOURNAL Submitted (25-JAN-1996) Melinda Brady, R&D, Promega Corporation,
5445 East Cheryl Parkway, Madison, WI 53711, USA
REFERENCE 3 (bases 1 to 4006)
AUTHORS Brondyk,W.H. and Groskreutz,D.J.
TITLE Direct Submission
JOURNAL Submitted (13-SEP-1999) Melinda Brady, R&D, Promega Corporation,
5445 East Cheryl Parkway, Madison, WI 53711, USA
REMARK Sequence update by submitter
COMMENT On Sep 13, 1999 this sequence version replaced U47119.1.
FEATURES Location/Qualifiers
source 1..4006
/organism="Cloning vector pCI"
/mol_type="genomic DNA"
/db_xref="taxon:45771"
regulatory 1..742
/regulatory_class="promoter"
/note="Cytomegalovirus immediate-early enhancer/promoter
region"
intron 857..989
/note="chimeric intron"
misc_feature 1020..1041
/note="T7-EEV sequencing primer binding site"
regulatory 1034..1052
/regulatory_class="promoter"
/note="T7 promoter"
misc_feature 1051
/note="T7 promoter transcription start site"
misc_feature 1052..1104
/note="multiple cloning sites"
regulatory 1111..1332
/regulatory_class="polyA_signal_sequence"
/note="SV40 late polyadenylation signal"
rep_origin 1422..1877
/note="phage f1 region"
gene 2314..3174
/gene="ampR"
CDS 2314..3174
/gene="ampR"
/codon_start=1
/product="beta-lactamase"
/protein_id="AAA88780.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
BASE COUNT 1018 a 956 c 955 g 1077 t
ORIGIN
1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
721 tcgtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat
781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc
841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa
901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac
961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta
1021 aggctagagt acttaatacg actcactata ggctagcctc gagaattcac gcgtggtacc
1081 tctagagtcg acccgggcgg ccgcttcgag cagacatgat aagatacatt gatgagtttg
1141 gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta
1201 ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc
1261 attttatgtt tcaggttcag ggggagatgt gggaggtttt ttaaagcaag taaaacctct
1321 acaaatgtgg taaaatcgat aaggatccgg gctggcgtaa tagcgaagag gcccgcaccg
1381 atcgcccttc ccaacagttg cgcagcctga atggcgaatg gacgcgccct gtagcggcgc
1441 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
1501 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
1561 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
1621 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
1681 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
1741 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc
1801 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat
1861 attaacgctt acaatttcct gatgcggtat tttctcctta cgcatctgtg cggtatttca
1921 caccgcatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagccc
1981 cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct
2041 tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca
2101 ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg
2161 ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
2221 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
2281 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
2341 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
2401 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
2461 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
2521 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
2581 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
2641 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
2701 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
2761 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
2821 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
2881 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
2941 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
3001 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
3061 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
3121 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
3181 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
3241 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
3301 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
3361 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
3421 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
3481 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
3541 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
3601 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
3661 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
3721 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
3781 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
3841 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
3901 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
3961 ttcctggcct tttgctggcc ttttgctcac atggctcgac agatct
//