LOCUS CVU36454 5782 bp DNA circular SYN 14-JUL-2016
DEFINITION Expression vector pCALn, complete sequence.
ACCESSION U36454
VERSION U36454.1
KEYWORDS .
SOURCE Cloning vector pCALn
ORGANISM Cloning vector pCALn
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 5782)
AUTHORS Simcox,T., Zheng,C.-F., Simcox,M.E. and Vaillancourt,P.
TITLE Calmodulin-Binding Peptide Affinity-Tag E.coli Protein Purification
System
JOURNAL Strateg. Mol. Biol. 8, 40-43 (1995)
REFERENCE 2 (bases 1 to 5782)
AUTHORS Vaillancourt,P.
TITLE Direct Submission
JOURNAL Submitted (15-SEP-1995) Peter Vaillancourt, R&D, Stratagene, 11011
North Torrey Pines Road, La Jolla, CA 92037, USA
FEATURES Location/Qualifiers
source 1..5782
/organism="Cloning vector pCALn"
/mol_type="genomic DNA"
/host="Escherichia coli"
/db_xref="taxon:43136"
/plasmid="pCALn"
/note="composite sequence, has not been sequenced"
CDS 940..2022
/codon_start=1
/transl_table=11
/product="lac operon repressor"
/protein_id="AAA79994.1"
/translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAE
LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS
GVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSII
FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG
DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT
EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNT
QTASPRALADSLMQLARQVSRLESGQ"
CDS complement(4714..5574)
/codon_start=1
/transl_table=11
/product="beta-lactamase"
/protein_id="AAA79995.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
BASE COUNT 1322 a 1569 c 1529 g 1362 t
ORIGIN
1 ttgtcatgtt tgacagctta tcttcgatta gctttaatgc ggtagtttat cacagttaaa
61 ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg
121 caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt
181 gcgggatatc cggatatagt tcctcctttc agcaaaaaac ccctcaagac ccgtttagag
241 gccccaaggg gttatgctag ttattgctca gcggtggcag cagccaactc agcttccttt
301 cgggctttgt tagcagccgg atctaagctt gagctcgagt cgacccatgg agtctagaat
361 tcccggggat ccacgcggaa ccagaagtgc cccggaggat gagattttct taaagcggtt
421 ggctgctgag acggctatga aattcttttt ccatcgtcgc ttcatatgta tatctccttc
481 ttaaagttaa acaaaattat ttctagaggg gaattgttat ccgctcacaa ttcccctata
541 gtgagtcgta ttaatttcgc gggatcgaga tctcgatcct ctacgccgga cgcatcgtgg
601 ccggcatcac cggcgccaca ggtgcggttg ctggcgccta tatcgccgac atcaccgatg
661 gggaagatcg ggctcgccac ttcgggctca tgagcgcttg tttcggcgtg ggtatggtgg
721 caggccccgt ggccggggga ctgttgggcg ccatctcctt gcatgcacca ttccttgcgg
781 cggcggtgct caacggcctc aacctactac tgggctgctt cctaatgcag gagtcgcata
841 agggagagcg tcgagatccc ggacaccatc gaatggcgca aaacctttcg cggtatggca
901 tgatagcgcc cggaagagag tcaattcagg gtggtgaatg tgaaaccagt aacgttatac
961 gatgtcgcag agtatgccgg tgtctcttat cagaccgttt cccgcgtggt gaaccaggcc
1021 agccacgttt ctgcgaaaac gcgggaaaaa gtggaagcgg cgatggcgga gctgaattac
1081 attcccaacc gcgtggcaca acaactggcg ggcaaacagt cgttgctgat tggcgttgcc
1141 acctccagtc tggccctgca cgcgccgtcg caaattgtcg cggcgattaa atctcgcgcc
1201 gatcaactgg gtgccagcgt ggtggtgtcg atggtagaac gaagcggcgt cgaagcctgt
1261 aaagcggcgg tgcacaatct tctcgcgcaa cgcgtcagtg ggctgatcat taactatccg
1321 ctggatgacc aggatgccat tgctgtggaa gctgcctgca ctaatgttcc ggcgttattt
1381 cttgatgtct ctgaccagac acccatcaac agtattattt tctcccatga agacggtacg
1441 cgactgggcg tggagcatct ggtcgcattg ggtcaccagc aaatcgcgct gttagcgggc
1501 ccattaagtt ctgtctcggc gcgtctgcgt ctggctggct ggcataaata tctcactcgc
1561 aatcaaattc agccgatagc ggaacgggaa ggcgactgga gtgccatgtc cggttttcaa
1621 caaaccatgc aaatgctgaa tgagggcatc gttcccactg cgatgctggt tgccaacgat
1681 cagatggcgc tgggcgcaat gcgcgccatt accgagtccg ggctgcgcgt tggtgcggat
1741 atctcggtag tgggatacga cgataccgaa gacagctcat gttatatccc gccgttaacc
1801 accatcaaac aggattttcg cctgctgggg caaaccagcg tggaccgctt gctgcaactc
1861 tctcagggcc aggcggtgaa gggcaatcag ctgttgcccg tctcactggt gaaaagaaaa
1921 accaccctgg cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
1981 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
2041 aagttagctc actcattagg caccgggatc tcgaccgatg cccttgagag ccttcaaccc
2101 agtcagctcc ttccggtggg cgcggggcat gactatcgtc gccgcactta tgactgtctt
2161 ctttatcatg caactcgtag gacaggtgcc ggcagcgctc tgggtcattt tcggcgagga
2221 ccgctttcgc tggagcgcga cgatgatcgg cctgtcgctt gcggtattcg gaatcttgca
2281 cgccctcgct caagccttcg tcactggtcc cgccaccaaa cgtttcggcg agaagcaggc
2341 cattatcgcc ggcatggcgg ccgacgcgct gggctacgtc ttgctggcgt tcgcgacgcg
2401 aggctggatg gccttcccca ttatgattct tctcgcttcc ggcggcatcg ggatgcccgc
2461 gttgcaggcc atgctgtcca ggcaggtaga tgacgaccat cagggacagc ttcaaggatc
2521 gctcgcggct cttaccagcc taacttcgat cactggaccg ctgatcgtca cggcgattta
2581 tgccgcctcg gcgagcacat ggaacgggtt ggcatggatt gtaggcgccg ccctatacct
2641 tgtctgcctc cccgcgttgc gtcgcggtgc atggagccgg gccacctcga cctgaatgga
2701 agccggcggc acctcgctaa cggattcacc actccaagaa ttggagccaa tcaattcttg
2761 cggagaactg tgaatgcgca aaccaaccct tggcagaaca tatccatcgc gtccgccatc
2821 tccagcagcc gcacgcggcg catctcgggc agcgttgggt cctggccacg ggtgcgcatg
2881 atcgtgctcc tgtcgttgag gacccggcta ggctggcggg gttgccttac tggttagcag
2941 aatgaatcac cgatacgcga gcgaacgtga agcgactgct gctgcaaaac gtctgcgacc
3001 tgagcaacaa catgaatggt cttcggtttc cgtgtttcgt aaagtctgga aacgcggaag
3061 tcagcgccct gcaccattat gttccggatc tgcatcgcag gatgctgctg gctaccctgt
3121 ggaacaccta catctgtatt aacgaagcgc tggcattgac cctgagtgat ttttctctgg
3181 tcccgccgca tccataccgc cagttgttta ccctcacaac gttccagtaa ccgggcatgt
3241 tcatcatcag taacccgtat cgtgagcatc ctctctcgtt tcatcggtat cattaccccc
3301 atgaacagaa atccccctta cacggaggca tcagtgacca aacaggaaaa aaccgccctt
3361 aacatggccc gctttatcag aagccagaca ttaacgcttc tggagaaact caacgagctg
3421 gacgcggatg aacaggcaga catctgtgaa tcgcttcacg accacgctga tgagctttac
3481 cgcagctgcc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg
3541 gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg
3601 tcagcgggtg ttggcgggtg tcggggcgca gccatgaccc agtcacgtag cgatagcgga
3661 gtgtatactg gcttaactat gcggcatcag agcagattgt actgagagtg caccatatat
3721 gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gctcttccgc
3781 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
3841 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
3901 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
3961 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
4021 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
4081 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
4141 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
4201 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
4261 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
4321 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
4381 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
4441 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
4501 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
4561 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
4621 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
4681 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
4741 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
4801 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
4861 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
4921 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
4981 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgctg caggcatcgt
5041 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
5101 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
5161 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
5221 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
5281 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa
5341 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
5401 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
5461 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
5521 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
5581 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
5641 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
5701 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac
5761 gaggcccttt cgtcttcaag aa
//