LOCUS CVU30496 8414 bp DNA circular SYN 13-DEC-1995 DEFINITION Cloning vector pAS2, complete sequence. ACCESSION U30496 VERSION U30496.1 KEYWORDS . SOURCE Cloning vector pAS2 ORGANISM Cloning vector pAS2 other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 8414) AUTHORS Kitts,P.A. TITLE CLONTECH Vectors On Disk, version 1.3 JOURNAL Unpublished REFERENCE 2 (bases 1 to 8414) AUTHORS Stile,J.S. TITLE Direct Submission JOURNAL Submitted (28-JUN-1995) John S. Stile, CLONTECH Laboratories, Inc., 4030 Fabian Way, Palo Alto, CA 94303, USA COMMENT This vector can be obtained from CLONTECH Laboratories, Inc., 4030 Fabian Way, Palo Alto, CA 94303, USA. To place an order call (415) 424-8222 or (800) 662-2566, extension 1. International customers, please contact your local distributor. For technical information, call (415) 424-8222 or (800) 662-2566, extension 3. This sequence has been compiled from information in the sequence databases, published literature and other sources, together with partial sequences obtained by CLONTECH. If you suspect there is an error in this sequence, please contact CLONTECH's Technical Service Department at (415) 424-8222 or (800) 662-2566, extension 3 or E-mail TECH@CLONTECH.COM. FEATURES Location/Qualifiers source 1..8414 /organism="Cloning vector pAS2" /mol_type="genomic DNA" /db_xref="taxon:41869" rep_origin 1..1348 /note="two micron origin of replication (B form)" CDS 1884..>2258 /codon_start=1 /transl_table=11 /product="TRP1" /protein_id="AAA83257.1" /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNR KRTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGD ESWQEYQEFLGLPVIKRLVFPKD" rep_origin 2609..3277 /note="f1+ origin" gene 4768..6244 /gene="ADH1" regulatory 4768..5475 /regulatory_class="promoter" /gene="ADH1" CDS 5502..>5942 /gene="ADH1" /note="GAL4 Binding Domain codons 1-147" /codon_start=1 /transl_table=11 /product="GAL4" /protein_id="AAA83258.1" /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKT KRSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNV NKDAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS" misc_feature 5945..6051 /gene="ADH1" /note="multiple cloning site" regulatory 6052..6244 /regulatory_class="terminator" /gene="ADH1" rep_origin 6252..7423 /note="pUC origin of replication" BASE COUNT 2353 a 1758 c 1878 g 2424 t ORIGIN 1 gaacgaagca tctgtgcttc attttgtaga acaaaaatgc aacgcgagag cgctaatttt 61 tcaaacaaag aatctgagct gcatttttac agaacagaaa tgcaacgcga aagcgctatt 121 ttaccaacga agaatctgtg cttcattttt gtaaaacaaa aatgcaacgc gagagcgcta 181 atttttcaaa caaagaatct gagctgcatt tttacagaac agaaatgcaa cgcgagagcg 241 ctattttacc aacaaagaat ctatacttct tttttgttct acaaaaatgc atcccgagag 301 cgctattttt ctaacaaagc atcttagatt actttttttc tcctttgtgc gctctataat 361 gcagtctctt gataactttt tgcactgtag gtccgttaag gttagaagaa ggctactttg 421 gtgtctattt tctcttccat aaaaaaagcc tgactccact tcccgcgttt actgattact 481 agcgaagctg cgggtgcatt ttttcaagat aaaggcatcc ccgattatat tctataccga 541 tgtggattgc gcatactttg tgaacagaaa gtgatagcgt tgatgattct tcattggtca 601 gaaaattatg aacggtttct tctattttgt ctctatatac tacgtatagg aaatgtttac 661 attttcgtat tgttttcgat tcactctatg aatagttctt actacaattt ttttgtctaa 721 agagtaatac tagagataaa cataaaaaat gtagaggtcg agtttagatg caagttcaag 781 gagcgaaagg tggatgggta ggttatatag ggatatagca cagagatata tagcaaagag 841 atacttttga gcaatgtttg tggaagcggt attcgcaata ttttagtagc tcgttacagt 901 ccggtgcgtt tttggttttt tgaaagtgcg tcttcagagc gcttttggtt ttcaaaagcg 961 ctctgaagtt cctatacttt ctagagaata ggaacttcgg aataggaact tcaaagcgtt 1021 tccgaaaacg agcgcttccg aaaatgcaac gcgagctgcg cacatacagc tcactgttca 1081 cgtcgcacct atatctgcgt gttgcctgta tatatatata catgagaaga acggcatagt 1141 gcgtgtttat gcttaaatgc gtacttatat gcgtctattt atgtaggatg aaaggtagtc 1201 tagtacctcc tgtgatatta tcccattcca tgcggggtat cgtatgcttc cttcagcact 1261 accctttagc tgttctatat gctgccactc ctcaattgga ttagtctcat ccttcaatgc 1321 tatcatttcc tttgatattg gatcatatta agaaaccatt attatcatga cattaaccta 1381 taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa 1441 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag 1501 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta 1561 tgcggcatca gagcagattg tactgagagt gcaccataga tcaacgacat tactatatat 1621 ataatatagg aagcatttaa tagacagcat cgtaatatat gtgtactttg cagttatgac 1681 gccagatggc agtagtggaa gatattcttt attgaaaaat agcttgtcac cttacgtaca 1741 atcttgatcc ggagcttttc tttttttgcc gattaagaat taattcggtc gaaaaaagaa 1801 aaggagaggg ccaagaggga gggcattggt gactattgag cacgtgagta tacgtgatta 1861 agcacacaaa ggcagcttgg agtatgtctg ttattaattt cacaggtagt tctggtccat 1921 tggtgaaagt ttgcggcttg cagagcacag aggccgcaga atgtgctcta gattccgatg 1981 ctgacttgct gggtattata tgtgtgccca atagaaagag aacaattgac ccggttattg 2041 caaggaaaat ttcaagtctt gtaaaagcat ataaaaatag ttcaggcact ccgaaatact 2101 tggttggcgt gtttcgtaat caacctaagg aggatgtttt ggctctggtc aatgattacg 2161 gcattgatat cgtccaactg catggagatg agtcgtggca agaataccaa gagttcctcg 2221 gtttgccagt tattaaaaga ctcgtatttc caaaagactg caacatacta ctcagtgcag 2281 cttcacagaa acctcattcg tttattccct tgtttgattc agaagcaggt gggacaggtg 2341 aacttttgga ttggaactcg atttctgact gggttggaag gcaagagagc cccgaaagct 2401 tacattttat gttagctggt ggactgacgc cagaaaatgt tggtgatgcg cttagattaa 2461 atggcgttat tggtgttgat gtaagcggag gtgtggagac aaatggtgta aaagactcta 2521 acaaaatagc aaatttcgtc aaaaatgcta agaaataggt tattactgag tagtatttat 2581 ttaagtattg tttgtgcact tgccgatcta tgcggtgtga aataccgcac agatgcgtaa 2641 ggagaaaata ccgcatcagg aaattgtaaa cgttaatatt ttgttaaaat tcgcgttaaa 2701 tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa 2761 atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact 2821 attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc 2881 actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa 2941 tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc 3001 gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt 3061 cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtcgcg 3121 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct 3181 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg 3241 gttttcccag tcacgacgtt gtaaaacgac ggccagtcgt ccaagctttc gcgagctcga 3301 gatcccgagc tttgcaaatt aaagccttcg agcgtcccaa aaccttctca agcaaggttt 3361 tcagtataat gttacatgcg tacacgcgtc tgtacagaaa aaaaagaaaa atttgaaata 3421 taaataacgt tcttaatact aacataacta taaaaaaata aatagggacc tagacttcag 3481 gttgtctaac tccttccttt tcggttagag cggatgtggg gggagggcgt gaatgtaagc 3541 gtgacataac taattacatg atatcgacaa aggaaaaggg gcctgtttac tcacaggctt 3601 ttttcaagta ggtaattaag tcgtttctgt ctttttcctt cttcaaccca ccaaaggcca 3661 tcttggtact tttttttttt tttttttttt tttttttttt tttttttttt tttttttttt 3721 tttttttttt tttttttttt tttttttttt tttttttttt tttcatagaa ataatacaga 3781 agtagatgtt gaattagatt aaactgaaga tatataattt attggaaaat acatagagct 3841 ttttgttgat gcgcttaagc gatcaattca acaacaccac cagcagctct gattttttct 3901 tcagccaact tggagacgaa tctagctttg acgataactg gaacatttgg aattctaccc 3961 ttacccaaga tcttaccgta accggctgcc aaagtgtcaa taactggagc agtttcctta 4021 gaagcagatt tcaagtattg gtctctcttg tcttctggga tcaatgtcca caatttgtcc 4081 aagttcaaga ctggcttcca gaaatgagct tgttgcttgt ggaagtatct cataccaanc 4141 cttaccgaaa taacctggat ggtatttatc catgttaatt ctgtggtgat gttgaccacc 4201 ggccatacct ctaccaccgg ggtgctttct gtgcttaccg atacgacctt taccggctga 4261 gacgtgacct ctgtgctttc tagtcttagt gaatctggaa ggcattcttg attagttgga 4321 tgattgttct gggatttaat gcaaaaaaat cactaagaag gaaaaaaatc aacggagaaa 4381 gcaaacgcca tcttaaatat acgggataca gatgaaaggt ttgaacctat ctgggaaaat 4441 acgcattaaa caagcgaaaa actgcgagga aaattgtttg cgtctctgcg ggctattcac 4501 gcgccagagg aaaataggaa aaataacagg gcattagaaa aataattttg attttggtaa 4561 tgtgtgggtc cctggtgtac agatgttaca ttggttacag tactcttgtt tttgctgtgt 4621 ttttcgatga atctccaaaa tggttgttag cacatggaag agtcaccgat gctaagttat 4681 ctctatgtaa gctacgtggc gtgacttttg atgaagccgc acaagagata caggattggc 4741 aactgcaaat agaatctggg gatctagata tccttttgtt gtttccgggt gtacaatatg 4801 gacttcctct tttctggcaa ccaaacccat acatcgggat tcctataata ccttcgttgg 4861 tctccctaac atgtaggtgg cggaggggag atatacaata gaacagatac cagacaagac 4921 ataatgggct aaacaagact acaccaatta cactgcctca ttgatggtgg tacataacga 4981 actaatactg tagccctaga cttgatagcc atcatcatat cgaagtttca ctaccctttt 5041 tccatttgcc atctattgaa gtaataatag gcgcatgcaa cttcttttct ttttttttct 5101 tttctctctc ccccgttgtt gtctcaccat atccgcaatg acaaaaaaaa tgatggaaga 5161 cactaaagga aaaaattaac gacaaagaca gcaccaacag atgtcgttgt tccagagctg 5221 atgaggggta tcttcgaaca cacgaaactt tttccttcct tcattcacgc acactactct 5281 ctaatgagca acggtatacg gccttccttc cagttacttg aatttgaaat aaaaaaagtt 5341 tgccgctttg ctatcaagta taaatagacc tgcaattatt aatcttttgt ttcctcgtca 5401 ttgttctcgt tccctttctt ccttgtttct ttttctgcac aatatttcaa gctataccaa 5461 gcatacaatc aactccaagc ttgaagcaag cctcctgaaa gatgaagcta ctgtcttcta 5521 tcgaacaagc atgcgatatt tgccgactta aaaagctcaa gtgctccaaa gaaaaaccga 5581 agtgcgccaa gtgtctgaag aacaactggg agtgtcgcta ctctcccaaa accaaaaggt 5641 ctccgctgac tagggcacat ctgacagaag tggaatcaag gctagaaaga ctggaacagc 5701 tatttctact gatttttcct cgagaagacc ttgacatgat tttgaaaatg gattctttac 5761 aggatataaa agcattgtta acaggattat ttgtacaaga taatgtgaat aaagatgccg 5821 tcacagatag attggcttca gtggagactg atatgcctct aacattgaga cagcatagaa 5881 taagtgcgac atcatcatcg gaagagagta gtaacaaagg tcaaagacag ttgactgtat 5941 cgccggattt catggcttac ccatacgatg ttccagatta cgctagcttg ggtggtcata 6001 tggccatgga ggccccgggg atccgtcgac ctgcagccaa gctaattccg ggcgaatttc 6061 ttatgattta tgatttttat tattaaataa gttataaaaa aaataagtgt atacaaattt 6121 taaagtgact cttaggtttt aaaacgaaaa ttcttgttct tgagtaactc tttcctgtag 6181 gtcaggttgc tttctcaggt atagcatgag gtcgctctta ttgaccacac ctctaccggc 6241 atgcaagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca 6301 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag 6361 tgaggtaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt 6421 cgtgccagct ggattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc 6481 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg 6541 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa 6601 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg 6661 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga 6721 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg 6781 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg 6841 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc 6901 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg 6961 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca 7021 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt 7081 ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag 7141 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg 7201 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc 7261 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt 7321 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt 7381 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca 7441 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg 7501 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac 7561 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg 7621 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc 7681 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta 7741 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac 7801 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc 7861 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac 7921 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact 7981 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa 8041 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt 8101 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca 8161 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa 8221 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac 8281 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg 8341 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc 8401 gaaaagtgcc acct //