LOCUS CVU30496 8414 bp DNA circular SYN 13-DEC-1995
DEFINITION Cloning vector pAS2, complete sequence.
ACCESSION U30496
VERSION U30496.1
KEYWORDS .
SOURCE Cloning vector pAS2
ORGANISM Cloning vector pAS2
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 8414)
AUTHORS Kitts,P.A.
TITLE CLONTECH Vectors On Disk, version 1.3
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 8414)
AUTHORS Stile,J.S.
TITLE Direct Submission
JOURNAL Submitted (28-JUN-1995) John S. Stile, CLONTECH Laboratories, Inc.,
4030 Fabian Way, Palo Alto, CA 94303, USA
COMMENT This vector can be obtained from CLONTECH Laboratories, Inc., 4030
Fabian Way, Palo Alto, CA 94303, USA. To place an order call (415)
424-8222 or (800) 662-2566, extension 1. International customers,
please contact your local distributor. For technical information,
call (415) 424-8222 or (800) 662-2566, extension 3. This sequence
has been compiled from information in the sequence databases,
published literature and other sources, together with partial
sequences obtained by CLONTECH. If you suspect there is an error in
this sequence, please contact CLONTECH's Technical Service
Department at (415) 424-8222 or (800) 662-2566, extension 3 or
E-mail TECH@CLONTECH.COM.
FEATURES Location/Qualifiers
source 1..8414
/organism="Cloning vector pAS2"
/mol_type="genomic DNA"
/db_xref="taxon:41869"
rep_origin 1..1348
/note="two micron origin of replication (B form)"
CDS 1884..>2258
/codon_start=1
/transl_table=11
/product="TRP1"
/protein_id="AAA83257.1"
/translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNR
KRTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGD
ESWQEYQEFLGLPVIKRLVFPKD"
rep_origin 2609..3277
/note="f1+ origin"
gene 4768..6244
/gene="ADH1"
regulatory 4768..5475
/regulatory_class="promoter"
/gene="ADH1"
CDS 5502..>5942
/gene="ADH1"
/note="GAL4 Binding Domain codons 1-147"
/codon_start=1
/transl_table=11
/product="GAL4"
/protein_id="AAA83258.1"
/translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKT
KRSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNV
NKDAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
misc_feature 5945..6051
/gene="ADH1"
/note="multiple cloning site"
regulatory 6052..6244
/regulatory_class="terminator"
/gene="ADH1"
rep_origin 6252..7423
/note="pUC origin of replication"
BASE COUNT 2353 a 1758 c 1878 g 2424 t
ORIGIN
1 gaacgaagca tctgtgcttc attttgtaga acaaaaatgc aacgcgagag cgctaatttt
61 tcaaacaaag aatctgagct gcatttttac agaacagaaa tgcaacgcga aagcgctatt
121 ttaccaacga agaatctgtg cttcattttt gtaaaacaaa aatgcaacgc gagagcgcta
181 atttttcaaa caaagaatct gagctgcatt tttacagaac agaaatgcaa cgcgagagcg
241 ctattttacc aacaaagaat ctatacttct tttttgttct acaaaaatgc atcccgagag
301 cgctattttt ctaacaaagc atcttagatt actttttttc tcctttgtgc gctctataat
361 gcagtctctt gataactttt tgcactgtag gtccgttaag gttagaagaa ggctactttg
421 gtgtctattt tctcttccat aaaaaaagcc tgactccact tcccgcgttt actgattact
481 agcgaagctg cgggtgcatt ttttcaagat aaaggcatcc ccgattatat tctataccga
541 tgtggattgc gcatactttg tgaacagaaa gtgatagcgt tgatgattct tcattggtca
601 gaaaattatg aacggtttct tctattttgt ctctatatac tacgtatagg aaatgtttac
661 attttcgtat tgttttcgat tcactctatg aatagttctt actacaattt ttttgtctaa
721 agagtaatac tagagataaa cataaaaaat gtagaggtcg agtttagatg caagttcaag
781 gagcgaaagg tggatgggta ggttatatag ggatatagca cagagatata tagcaaagag
841 atacttttga gcaatgtttg tggaagcggt attcgcaata ttttagtagc tcgttacagt
901 ccggtgcgtt tttggttttt tgaaagtgcg tcttcagagc gcttttggtt ttcaaaagcg
961 ctctgaagtt cctatacttt ctagagaata ggaacttcgg aataggaact tcaaagcgtt
1021 tccgaaaacg agcgcttccg aaaatgcaac gcgagctgcg cacatacagc tcactgttca
1081 cgtcgcacct atatctgcgt gttgcctgta tatatatata catgagaaga acggcatagt
1141 gcgtgtttat gcttaaatgc gtacttatat gcgtctattt atgtaggatg aaaggtagtc
1201 tagtacctcc tgtgatatta tcccattcca tgcggggtat cgtatgcttc cttcagcact
1261 accctttagc tgttctatat gctgccactc ctcaattgga ttagtctcat ccttcaatgc
1321 tatcatttcc tttgatattg gatcatatta agaaaccatt attatcatga cattaaccta
1381 taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa
1441 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag
1501 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta
1561 tgcggcatca gagcagattg tactgagagt gcaccataga tcaacgacat tactatatat
1621 ataatatagg aagcatttaa tagacagcat cgtaatatat gtgtactttg cagttatgac
1681 gccagatggc agtagtggaa gatattcttt attgaaaaat agcttgtcac cttacgtaca
1741 atcttgatcc ggagcttttc tttttttgcc gattaagaat taattcggtc gaaaaaagaa
1801 aaggagaggg ccaagaggga gggcattggt gactattgag cacgtgagta tacgtgatta
1861 agcacacaaa ggcagcttgg agtatgtctg ttattaattt cacaggtagt tctggtccat
1921 tggtgaaagt ttgcggcttg cagagcacag aggccgcaga atgtgctcta gattccgatg
1981 ctgacttgct gggtattata tgtgtgccca atagaaagag aacaattgac ccggttattg
2041 caaggaaaat ttcaagtctt gtaaaagcat ataaaaatag ttcaggcact ccgaaatact
2101 tggttggcgt gtttcgtaat caacctaagg aggatgtttt ggctctggtc aatgattacg
2161 gcattgatat cgtccaactg catggagatg agtcgtggca agaataccaa gagttcctcg
2221 gtttgccagt tattaaaaga ctcgtatttc caaaagactg caacatacta ctcagtgcag
2281 cttcacagaa acctcattcg tttattccct tgtttgattc agaagcaggt gggacaggtg
2341 aacttttgga ttggaactcg atttctgact gggttggaag gcaagagagc cccgaaagct
2401 tacattttat gttagctggt ggactgacgc cagaaaatgt tggtgatgcg cttagattaa
2461 atggcgttat tggtgttgat gtaagcggag gtgtggagac aaatggtgta aaagactcta
2521 acaaaatagc aaatttcgtc aaaaatgcta agaaataggt tattactgag tagtatttat
2581 ttaagtattg tttgtgcact tgccgatcta tgcggtgtga aataccgcac agatgcgtaa
2641 ggagaaaata ccgcatcagg aaattgtaaa cgttaatatt ttgttaaaat tcgcgttaaa
2701 tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa
2761 atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact
2821 attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc
2881 actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa
2941 tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc
3001 gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt
3061 cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtcgcg
3121 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
3181 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
3241 gttttcccag tcacgacgtt gtaaaacgac ggccagtcgt ccaagctttc gcgagctcga
3301 gatcccgagc tttgcaaatt aaagccttcg agcgtcccaa aaccttctca agcaaggttt
3361 tcagtataat gttacatgcg tacacgcgtc tgtacagaaa aaaaagaaaa atttgaaata
3421 taaataacgt tcttaatact aacataacta taaaaaaata aatagggacc tagacttcag
3481 gttgtctaac tccttccttt tcggttagag cggatgtggg gggagggcgt gaatgtaagc
3541 gtgacataac taattacatg atatcgacaa aggaaaaggg gcctgtttac tcacaggctt
3601 ttttcaagta ggtaattaag tcgtttctgt ctttttcctt cttcaaccca ccaaaggcca
3661 tcttggtact tttttttttt tttttttttt tttttttttt tttttttttt tttttttttt
3721 tttttttttt tttttttttt tttttttttt tttttttttt tttcatagaa ataatacaga
3781 agtagatgtt gaattagatt aaactgaaga tatataattt attggaaaat acatagagct
3841 ttttgttgat gcgcttaagc gatcaattca acaacaccac cagcagctct gattttttct
3901 tcagccaact tggagacgaa tctagctttg acgataactg gaacatttgg aattctaccc
3961 ttacccaaga tcttaccgta accggctgcc aaagtgtcaa taactggagc agtttcctta
4021 gaagcagatt tcaagtattg gtctctcttg tcttctggga tcaatgtcca caatttgtcc
4081 aagttcaaga ctggcttcca gaaatgagct tgttgcttgt ggaagtatct cataccaanc
4141 cttaccgaaa taacctggat ggtatttatc catgttaatt ctgtggtgat gttgaccacc
4201 ggccatacct ctaccaccgg ggtgctttct gtgcttaccg atacgacctt taccggctga
4261 gacgtgacct ctgtgctttc tagtcttagt gaatctggaa ggcattcttg attagttgga
4321 tgattgttct gggatttaat gcaaaaaaat cactaagaag gaaaaaaatc aacggagaaa
4381 gcaaacgcca tcttaaatat acgggataca gatgaaaggt ttgaacctat ctgggaaaat
4441 acgcattaaa caagcgaaaa actgcgagga aaattgtttg cgtctctgcg ggctattcac
4501 gcgccagagg aaaataggaa aaataacagg gcattagaaa aataattttg attttggtaa
4561 tgtgtgggtc cctggtgtac agatgttaca ttggttacag tactcttgtt tttgctgtgt
4621 ttttcgatga atctccaaaa tggttgttag cacatggaag agtcaccgat gctaagttat
4681 ctctatgtaa gctacgtggc gtgacttttg atgaagccgc acaagagata caggattggc
4741 aactgcaaat agaatctggg gatctagata tccttttgtt gtttccgggt gtacaatatg
4801 gacttcctct tttctggcaa ccaaacccat acatcgggat tcctataata ccttcgttgg
4861 tctccctaac atgtaggtgg cggaggggag atatacaata gaacagatac cagacaagac
4921 ataatgggct aaacaagact acaccaatta cactgcctca ttgatggtgg tacataacga
4981 actaatactg tagccctaga cttgatagcc atcatcatat cgaagtttca ctaccctttt
5041 tccatttgcc atctattgaa gtaataatag gcgcatgcaa cttcttttct ttttttttct
5101 tttctctctc ccccgttgtt gtctcaccat atccgcaatg acaaaaaaaa tgatggaaga
5161 cactaaagga aaaaattaac gacaaagaca gcaccaacag atgtcgttgt tccagagctg
5221 atgaggggta tcttcgaaca cacgaaactt tttccttcct tcattcacgc acactactct
5281 ctaatgagca acggtatacg gccttccttc cagttacttg aatttgaaat aaaaaaagtt
5341 tgccgctttg ctatcaagta taaatagacc tgcaattatt aatcttttgt ttcctcgtca
5401 ttgttctcgt tccctttctt ccttgtttct ttttctgcac aatatttcaa gctataccaa
5461 gcatacaatc aactccaagc ttgaagcaag cctcctgaaa gatgaagcta ctgtcttcta
5521 tcgaacaagc atgcgatatt tgccgactta aaaagctcaa gtgctccaaa gaaaaaccga
5581 agtgcgccaa gtgtctgaag aacaactggg agtgtcgcta ctctcccaaa accaaaaggt
5641 ctccgctgac tagggcacat ctgacagaag tggaatcaag gctagaaaga ctggaacagc
5701 tatttctact gatttttcct cgagaagacc ttgacatgat tttgaaaatg gattctttac
5761 aggatataaa agcattgtta acaggattat ttgtacaaga taatgtgaat aaagatgccg
5821 tcacagatag attggcttca gtggagactg atatgcctct aacattgaga cagcatagaa
5881 taagtgcgac atcatcatcg gaagagagta gtaacaaagg tcaaagacag ttgactgtat
5941 cgccggattt catggcttac ccatacgatg ttccagatta cgctagcttg ggtggtcata
6001 tggccatgga ggccccgggg atccgtcgac ctgcagccaa gctaattccg ggcgaatttc
6061 ttatgattta tgatttttat tattaaataa gttataaaaa aaataagtgt atacaaattt
6121 taaagtgact cttaggtttt aaaacgaaaa ttcttgttct tgagtaactc tttcctgtag
6181 gtcaggttgc tttctcaggt atagcatgag gtcgctctta ttgaccacac ctctaccggc
6241 atgcaagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
6301 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
6361 tgaggtaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
6421 cgtgccagct ggattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
6481 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
6541 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
6601 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
6661 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
6721 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
6781 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
6841 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
6901 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
6961 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
7021 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
7081 ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag
7141 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
7201 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
7261 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
7321 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
7381 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
7441 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
7501 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
7561 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
7621 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
7681 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
7741 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
7801 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
7861 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
7921 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
7981 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
8041 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
8101 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
8161 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
8221 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
8281 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
8341 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
8401 gaaaagtgcc acct
//