LOCUS PPU30471 5500 bp DNA linear BCT 14-JUL-2017
DEFINITION Plasmid pSa osa operon, replicase (repA), nuclease (nuc), and
oncogenic suppressive protein (osa) genes, complete cds.
ACCESSION U30471 M61749 M81325 M95790
VERSION U30471.1
KEYWORDS .
SOURCE Plasmid pSa
ORGANISM Plasmid pSa
other sequences; plasmids.
REFERENCE 1 (bases 1 to 5500)
AUTHORS Lee,L.-Y. and Kado,C.I.
TITLE Physical and Genetic Map of the Region Flanking the Oncogenic
Suppressive Gene osa of Plasmid pSa
JOURNAL Unpublished
REFERENCE 2 (bases 3948 to 5462)
AUTHORS Close,S.M. and Kado,C.I.
TITLE The osa gene of pSa encodes a 21.1-kilodalton protein that
suppresses Agrobacterium tumefaciens oncogenicity
JOURNAL J. Bacteriol. 173 (17), 5449-5456 (1991)
PUBMED 1832152
REFERENCE 3 (bases 2553 to 3947)
AUTHORS Close,S.M. and Kado,C.I.
TITLE A gene near the plasmid pSa origin of replication encodes a
nuclease
JOURNAL Mol. Microbiol. 6 (4), 521-527 (1992)
PUBMED 1560781
REFERENCE 4 (bases 414 to 2552)
AUTHORS Okumura,M.S. and Kado,C.I.
TITLE The region essential for efficient autonomous replication of pSa in
Escherichia coli
JOURNAL Mol. Gen. Genet. 235 (1), 55-63 (1992)
PUBMED 1435731
REFERENCE 5 (bases 1 to 5500)
AUTHORS Lee,L.-Y.
TITLE Direct Submission
JOURNAL Submitted (27-JUN-1995) Lan-Ying Lee, Plant Pathology, University
of California, Davis, Davis, CA 95616, USA
COMMENT On or before Sep 7, 1995 this sequence version replaced gi:150811,
gi:150819.
FEATURES Location/Qualifiers
source 1..5500
/organism="Plasmid pSa"
/plasmid=""
/mol_type="genomic DNA"
/host="Shigella flexneri"
/db_xref="taxon:2637"
/map="10-15 kb region of pSa 39.5 kb"
/clone="pUCD5409"
CDS 459..1115
/note="orf2"
/codon_start=1
/transl_table=11
/protein_id="AAA75243.1"
/translation="MPKYYAYLRVSRDGQDPENQKYGLLEYANAKGFAPLQIEEEIAS
RAKDWRKRKLGAIIEKAERGDVLLTPEITRIAGSALAALEILKAASERGLIVHVTKQK
IIMDGSLQSDIMATVLGLAAQIERHFIQARTTEALQVARERGKTLGRPKGSKSSALKL
DSRIDEVQAYVNLGLPQSRAAELLGVSPHTLRLFIKRRNIKPTNTRPTITMPGREQHA
"
gene 1108..2079
/gene="repA"
CDS 1108..2079
/gene="repA"
/function="replicase"
/codon_start=1
/transl_table=11
/product="RepA"
/protein_id="AAA75244.1"
/translation="MPKNNKAPGHRINEIIKTSLALEMEDAREAGLVGYMARCLVQAT
MPHTDPKTSYFERTNGIVTLSIMGKPSIGLPYGSMPRTLLAWICTEAVRTKDPVLNLG
RSQSEFLQRLGMHTDGRYTATLRNQAQRLFSSMISLAGEQGNDFGIENVVIAKRAFLF
WNPKRPEDRALWDSTLTLTGDFFEEVTRSPVPIRIDYLHALRQSPLAMDIYTWLTYRV
FLLRAKGRPFVQIPWVALQAQFGSSYGSRARNSPELDDKARERAERAALASFKYNFKK
RLREVLIVYPEASDCIEDDGECLRIKSTRLHVTRAPGKGARIGPPPT"
rep_origin 2170..2690
CDS 2797..3426
/note="orf1"
/codon_start=1
/transl_table=11
/protein_id="AAA75245.1"
/translation="MAITKQDIWRAADELDAEGIRPTLAAVRKKLGSGSFTTISDAMA
EWKNRKTATLPSSDPLPVAVNEHLAELGNALWAIALAHANARFDEDRKQIEADKAAIS
QQLAEAIELADTFTRENDQLRERVNQLEPMERERDKLADQLAEVKRRSGEELNRCMEK
LTQRDNEAIEARKQAKEAIERAASLQGQVEALKEQVANLTAVLKTGGKQ"
gene 3423..3947
/gene="nuc"
CDS 3423..3947
/gene="nuc"
/function="DNase"
/note="similar to plasmid RP4 ParB protein, encoded by
GenBank Accession Number M59825; periplasmic or secreted"
/codon_start=1
/transl_table=11
/product="nuclease"
/protein_id="AAA75246.1"
/translation="MKSALAALRAVAAAVVLIVSVPAWADFRGEVVRILDGDTIDVLV
NRQTIRVRLADIDAPESGQAFGSRARQRLADLTFRQEVQVTEKEVDRYGRTLGVVYAP
LQYPGGQTQLTNINAIMVQEGMAWAYRYYGKPTDAQMYEYEKEARRQRLGLWSDPNAQ
EPWKWRRASKNATN"
CDS 4151..4753
/note="orf3"
/codon_start=1
/transl_table=11
/protein_id="AAA75247.1"
/translation="MTDTRREQEKDERRKLQEQSRQNEAETMRLLAFEAGRQLAEIPK
EAKGNEPLLENYKSGLQETRKELETTPDATKSTNANRLERDVERAIIEAQQVREAVGR
EKARADEFHRHAEPGETYRGRVIGRTNSYVIQADDSRPGTIILHERAAVSGAEKVKMN
DHAEISYPHGRAGIVRNPQAAQHQRQRQMEKTGAGREHGR"
gene 4756..5325
/gene="osa"
CDS 4756..5325
/gene="osa"
/note="suppresses Agrobacterium tumefaciens oncogenicity"
/codon_start=1
/transl_table=11
/product="OSA protein"
/protein_id="AAA75248.1"
/translation="MLMLLRRRCRAWLEIRRLDKELAQSSGLPLELPQIVPNAWNEVV
WRLPVPNHPDAFMTASNAAQSDFIVYVNGLAFYRAWLALGVEDSQACPLKQDMPKDRK
YPSSAAHFAVGIDSPVPLADVSPTMILGHFAVCFTDGMTRSMWLLAHEVAVFPVLSRD
EASAVMLAEHVGVAAPIQVSKLREQCRKI"
BASE COUNT 1302 a 1659 c 1491 g 1048 t
ORIGIN
1 ggatccaacc cctccgctgc tatagtgcag tcggcttctg acgttcagtg cagccgtctt
61 ctgaaaacga catctggtgt tcctcattct ctggtggctg cccggcccgg tgcgctttct
121 tctcggcttg gcttcgttcg ccagtctcat caccctaccg atcatgtggc ttgggctgga
181 ctcggccaat aagacccaca tcatgctatc ggtcgcaggc gtcggccttg gtgcgtcggc
241 cctcatgtgg ttttatgacg cgcttctcat gaggctggca ccagaaccaa tcctattcag
301 cacctaaacg aaggccgggc cactcacccg gccttttttg tacgctcata gggcagaaca
361 aaccaacgtt ttatctatac cgctacaggg tatttaattc ctatttaatc tgcgctagaa
421 tgaggcatgt ttaaccgaat ctgacgtttt ccctgcaaat gccaaaatac tatgcctatc
481 tccgggtttc gcgtgacggc caagacccgg aaaaccaaaa atacggtttg ctcgaatacg
541 cgaacgccaa aggcttcgcg ccgctacaga tcgaggaaga aattgccagc agagcaaagg
601 actggcgcaa gcgcaagctc ggagcaatca tcgaaaaggc cgagcgtggc gacgtgctac
661 tgacgccgga gattacgcgc attgccggtt ccgccctcgc cgccttggaa attctcaaag
721 cggcgagcga gcgcggccta atcgtccatg tgaccaaaca gaagatcatc atggacggca
781 gcctacaaag cgacatcatg gcaaccgtgc ttggcttggc tgcacagatc gagcggcatt
841 tcattcaggc acgtaccacc gaggcgctac aagtcgccag agagcgcggc aagacgctcg
901 ggcgacccaa gggcagcaaa tcgagcgcct tgaagctgga cagccgtatt gatgaagtac
961 aggcatacgt gaaccttggc ttgccgcaaa gtcgcgcagc cgagttgtta ggcgtcagcc
1021 ctcacacctt gcgcctgttc atcaaacgcc ggaacatcaa acccacaaac actagaccaa
1081 ccatcaccat gccggggagg gaacaacatg cctaagaaca acaaagcccc cggccatcgt
1141 atcaacgaga tcatcaagac gagcctcgcg ctcgaaatgg aggatgcccg cgaagctggc
1201 ttagtcggct acatggcccg ttgccttgtg caagcgacca tgccccacac cgaccccaag
1261 accagctact ttgagcgcac caatggcatc gtcaccttgt cgatcatggg caagccgagc
1321 atcggcctgc cctacggttc tatgccgcgc accttgcttg cttggatatg caccgaggcc
1381 gtgcgaacga aagaccccgt gttgaacctt ggccggtcgc aatcggaatt tctacaaagg
1441 ctcggaatgc acaccgatgg ccgttacacg gccacccttc gcaatcaggc gcaacgcctg
1501 ttttcatcca tgatttcgct tgccggcgag caaggcaatg acttcggcat tgagaacgtc
1561 gtcattgcca agcgcgcttt tctattctgg aatcccaagc ggccagaaga tcgggcgcta
1621 tgggatagca ccctcaccct cacaggcgat ttcttcgagg aagtcacccg ctcaccggtt
1681 cctatccgaa tcgactacct gcatgccttg cggcagtctc cgcttgcgat ggacatttac
1741 acgtggctga cctatcgcgt gttcctgttg cgggccaagg gccgcccctt cgtgcaaatc
1801 ccttgggtcg ccctgcaagc gcaattcggc tcatcctatg gcagccgcgc acgcaactcg
1861 cccgaactgg acgataaggc ccgagagcgg gcagagcggg cagcactcgc cagcttcaaa
1921 tacaacttca aaaagcgcct acgcgaagtg ttgattgtct atcccgaggc aagcgactgc
1981 atcgaagatg acggcgaatg cctgcgcatc aaatccacac gcctgcatgt cacccgcgca
2041 cccggcaagg gcgctcgcat cggcccccct ccgacttgac caggccaacg ctacgcttgg
2101 cttggtcaag ccttcccatc caacagcccg ccgtcgagcg ggctttttta tccccggaag
2161 cctgtggata gagggtagtt atccacgtga aaccgctaat gccccgcaaa gccttgattc
2221 acggggcttt ccggcccgct ccaaaaacta tccacgtgaa atcgctaatc agggtacgtg
2281 aaatcgctaa tcggagtacg tgaaatcgct aataaggtca cgtgaaatcg ctaatcaaaa
2341 aggcacgtga gaacgctaat agccctttca gatcaacagc ttgcaaacac ccctcgctcc
2401 ggcaagtagt tacagcaagt agtatgttca attagctttt caattatgaa tatatatatc
2461 aattattggt cgcccttggc ttgtggacaa tgcgctacgc gcaccggctc cgcccgtgga
2521 caaccgcaag cggttgccca ccgtcgagcg ccagcgcctt tgcccacaac ccggcggccg
2581 caacagatcg ttttataaat tttttttttt gaaaaagaaa aagcccgaaa ggcggcaacc
2641 tctcgggctt ctggatttcc gatcaacgca ggagtcgttc ggaaagtagc tgttccagaa
2701 ttataggcgc agagacacca gattccaaga tggctctgtt aaattgttgt agtatgtagt
2761 atcatacaac atactacagt acagaggccc gcaagaatgg caatcactaa acaagacatt
2821 tggcgagcag ccgacgaact ggacgccgaa ggcatccggc ccactttggc cgccgtgcgc
2881 aagaaactcg gaagcggtag cttcacaacc atttccgatg caatggctga atggaaaaac
2941 cgcaagaccg ccaccctgcc ctcatcagac ccattgccgg ttgcagtcaa cgagcatctt
3001 gccgagcttg gcaatgcgct atgggctatc gccctggcgc acgccaacgc ccggtttgac
3061 gaagatcgga aacagatcga ggccgacaaa gcggccatca gccagcagct tgccgaagca
3121 atcgaactag ccgacacctt cacccgcgaa aacgaccagc tccgcgaacg agtgaatcag
3181 ctcgaaccta tggaacgcga gcgcgacaag ctggccgacc agcttgccga agtgaaacgc
3241 cgcagcggcg aagaactaaa ccgctgcatg gaaaagctca cccaacgcga taacgaggct
3301 atcgaggccc gcaaacaggc caaggaggcc atcgagcgcg ccgccagtct gcaaggtcag
3361 gtggaagccc tcaaagagca ggtcgccaat ctcacagccg tcttgaaaac aggaggcaaa
3421 caatgaaaag cgcccttgcc gcccttcgcg cggtcgcggc cgctgtcgtc ctaatcgtca
3481 gtgtgcccgc ttgggccgac ttccggggtg aagtcgtccg aatccttgac ggtgacacta
3541 tcgacgtttt ggtgaaccgt cagaccatcc gcgtgagatt ggccgatatt gacgcaccgg
3601 aaagcggcca agccttcggc tcccgtgctc gccaacggct cgccgacttg acctttcgcc
3661 aagaggttca agtgaccgaa aaagaggttg atcggtatgg ccgcactctt ggggtcgttt
3721 acgcgccgtt gcaatacccc ggcggccaaa cacaactcac caacatcaat gcgatcatgg
3781 ttcaagaagg catggcctgg gcttaccgtt attacggcaa accaaccgac gcgcagatgt
3841 acgagtatga aaaagaggcc cgccgccaac ggctcggcct ttggtcagac ccgaatgctc
3901 aggagccttg gaaatggcgt cgcgcctcga aaaatgccac gaactgacac cgggcacgcc
3961 ccttgttcga cgcgccgcag gcacgtcgaa tttaccgccg ggacgcccct cgtcccgaca
4021 cttccagatc gccatagcgc acagcgccga gtcacccgaa gggccgcaac gtagtggagg
4081 acggcaacgc cgttgaacgg cgcgagcact atggcacgcg aagcgagctt gacacgatgg
4141 aggaaagacc atgaccgaca cgcgccgcga gcaggagaaa gacgaacgcc ggaagctgca
4201 agagcagtcg cgccagaatg aagcggaaac catgcgcctg ctggctttcg aggcaggccg
4261 ccaattggcc gaaattccga aagaagccaa aggcaatgaa cccttgcttg agaactacaa
4321 gagcggacta caagagaccc gcaaagagct agaaaccacg ccagacgcca ctaagagcac
4381 caacgccaac cggcttgagc gcgacgtaga aagggccatc atcgaggcgc aacaggtgcg
4441 cgaggcagta ggcagggaga aagcccgcgc agatgaattt caccgccacg cagaaccggg
4501 cgaaacttac cggggccgtg tgatcggtcg caccaatagc tatgtcattc aggccgacga
4561 tagccgccca ggcacgatca ttctgcatga acgcgccgct gtttcgggcg cggagaaagt
4621 gaagatgaac gaccatgcgg aaatcagtta tccgcacggt cgcgccggaa tcgtccgcaa
4681 cccacaggcc gcgcaacatc aacgacagcg gcagatggaa aaaaccggcg caggccgaga
4741 gcatgggcgc taacgatgct gatgttgcta cggcggcggt gtcgcgcttg gctggaaatt
4801 cggcggcttg ataaagagct tgcgcagtcg agcgggttgc ccctcgaatt gcctcaaatc
4861 gtgccgaatg cttggaatga ggttgtttgg cggctgcccg tgccgaacca tcctgacgcc
4921 tttatgacag cttcaaatgc cgcacagtcc gatttcattg tgtatgtgaa tggcttggct
4981 ttctatcgag cgtggttggc gttaggcgtc gaggattctc aagcctgccc gttgaagcag
5041 gacatgccta aagaccggaa atatccgtcg agcgccgcgc attttgccgt gggcatcgac
5101 agccccgttc cgcttgctga cgtaagcccg accatgattc tgggccattt cgcggtgtgt
5161 ttcactgatg gcatgacccg ttcaatgtgg cttttggccc atgaggtcgc cgtgtttccg
5221 gtgctttccc gtgatgaagc atccgccgtt atgttggcag aacacgtggg cgtagcagcg
5281 ccgattcagg tttcaaagct gcgtgagcaa tgcaggaaga tctagggcgt gtccctgcgc
5341 tttgctccgg gccgtgctct attccgtttg acctggccgc cgctgcgctc tggctaggtc
5401 aaactccacc gaaccccctt cggggtttcg gcccattcgg gtaacgatca ctcacgcctc
5461 cgggccgctg tagcgtccct gcgcgcccct atcggggctt
//