LOCUS PRS413 4970 bp DNA circular SYN 24-MAY-1995
DEFINITION Yeast centromere vector pRS413 with HIS3 marker, complete sequence.
ACCESSION U03447
VERSION U03447.1
KEYWORDS .
SOURCE Cloning vector pRS413
ORGANISM Cloning vector pRS413
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 4970)
AUTHORS Sikorski,R.S. and Hieter,P.
TITLE A system of shuttle vectors and yeast host strains designed for
efficient manipulation of DNA in Saccharomyces cerevisiae
JOURNAL Genetics 122 (1), 19-27 (1989)
PUBMED 2659436
REFERENCE 2 (bases 1 to 4970)
AUTHORS Christianson,T.W., Sikorski,R.S., Dante,M., Shero,J.H. and
Hieter,P.
TITLE Multifunctional yeast high-copy-number shuttle vectors
JOURNAL Gene 110 (1), 119-122 (1992)
PUBMED 1544568
REFERENCE 3 (bases 1 to 4970)
AUTHORS Stillman,D.J.
TITLE Direct Submission
JOURNAL Submitted (11-NOV-1993) David J. Stillman, Dept. of Cellular, Viral
and Molecular Biology, University of Utah Medical Center, Salt Lake
City, UT 84132 USA
FEATURES Location/Qualifiers
source 1..4970
/organism="Cloning vector pRS413"
/mol_type="genomic DNA"
/db_xref="taxon:31838"
BASE COUNT 1331 a 1140 c 1170 g 1329 t
ORIGIN
1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgcgtg
121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
181 accataaatt cccgttttaa gagcttggtg agcgctagga gtcactgcca ggtatcgttt
241 gaacacggca ttagtcaggg aagtcataac acagtccttt cccgcaattt tctttttcta
301 ttactcttgg cctcctctag tacactctat atttttttat gcctcggtaa tgattttcat
361 tttttttttt cccctagcgg atgactcttt ttttttctta gcgattggca ttatcacata
421 atgaattata cattatataa agtaatgtga tttcttcgaa gaatatacta aaaaatgagc
481 aggcaagata aacgaaggca aagatgacag agcagaaagc cctagtaaag cgtattacaa
541 atgaaaccaa gattcagatt gcgatctctt taaagggtgg tcccctagcg atagagcact
601 cgatcttccc agaaaaagag gcagaagcag tagcagaaca ggccacacaa tcgcaagtga
661 ttaacgtcca cacaggtata gggtttctgg accatatgat acatgctctg gccaagcatt
721 ccggctggtc gctaatcgtt gagtgcattg gtgacttaca catagacgac catcacacca
781 ctgaagactg cgggattgct ctcggtcaag cttttaaaga ggccctactg gcgcgtggag
841 taaaaaggtt tggatcagga tttgcgcctt tggatgaggc actttccaga gcggtggtag
901 atctttcgaa caggccgtac gcagttgtcg aacttggttt gcaaagggag aaagtaggag
961 atctctcttg cgagatgatc ccgcattttc ttgaaagctt tgcagaggct agcagaatta
1021 ccctccacgt tgattgtctg cgaggcaaga atgatcatca ccgtagtgag agtgcgttca
1081 aggctcttgc ggttgccata agagaagcca cctcgcccaa tggtaccaac gatgttccct
1141 ccaccaaagg tgttcttatg tagtgacacc gattatttaa agctgcagca tacgatatat
1201 atacatgtgt atatatgtat acctatgaat gtcagtaagt atgtatacga acagtatgat
1261 actgaagatg acaaggtaat gcatcattct atacgtgtca ttctgaacga ggcgcgcttt
1321 ccttttttct ttttgctttt tctttttttt tctcttgaac tcgacggatc tatgcggtgt
1381 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggaaattgta aacgttaata
1441 ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg
1501 aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc
1561 cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa
1621 ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt
1681 cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac
1741 ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta
1801 gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg
1861 cgccgctaca gggcgcgtcg cgccattcgc cattcaggct gcgcaactgt tgggaagggc
1921 gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc
1981 gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg
2041 agcgcgcgta atacgactca ctatagggcg aattgggtac cgggcccccc ctcgaggtcg
2101 acggtatcga taagcttgat atcgaattcc tgcagcccgg gggatccact agttctagag
2161 cggccgccac cgcggtggag ctccagcttt tgttcccttt agtgagggtt aattgcgcgc
2221 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
2281 cacaacatag gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgaggtaa
2341 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
2401 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
2461 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
2521 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
2581 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
2641 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
2701 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
2761 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
2821 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
2881 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
2941 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
3001 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
3061 tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc
3121 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
3181 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
3241 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
3301 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
3361 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
3421 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
3481 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
3541 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
3601 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
3661 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
3721 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
3781 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
3841 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
3901 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
3961 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
4021 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
4081 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
4141 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
4201 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
4261 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
4321 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
4381 ccacctgggt ccttttcatc acgtgctata aaaataatta taatttaaat tttttaatat
4441 aaatatataa attaaaaata gaaagtaaaa aaagaaatta aagaaaaaat agtttttgtt
4501 ttccgaagat gtaaaagact ctagggggat cgccaacaaa tactaccttt tatcttgctc
4561 ttcctgctct caggtattaa tgccgaattg tttcatcttg tctgtgtaga agaccacaca
4621 cgaaaatcct gtgattttac attttactta tcgttaatcg aatgtatatc tatttaatct
4681 gcttttcttg tctaataaat atatatgtaa agtacgcttt ttgttgaaat tttttaaacc
4741 tttgtttatt tttttttctt cattccgtaa ctcttctacc ttctttattt actttctaaa
4801 atccaaatac aaaacataaa aataaataaa cacagagtaa attcccaaat tattccatca
4861 ttaaaagata cgaggcgcgt gtaagttaca ggcaagcgat ccgtcctaag aaaccattat
4921 tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc
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