LOCUS MT896414 5545 bp DNA circular SYN 03-NOV-2020
DEFINITION Cloning vector pG20_FLAG_Hyg, complete sequence.
ACCESSION MT896414
VERSION MT896414.1
KEYWORDS .
SOURCE Cloning vector pG20_FLAG_Hyg
ORGANISM Cloning vector pG20_FLAG_Hyg
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 5545)
AUTHORS Pratt,A.I., Knoblauch,J. and Kunz,H.-H.
TITLE An updated pGREEN-based vector suite for cost-effective cloning in
plant molecular biology
JOURNAL MicroPubl Biol (2020)
REMARK Publication Status: Online-Only
DOI: 10.17912/micropub.biology.000317
REFERENCE 2 (bases 1 to 5545)
AUTHORS Pratt,A.I. and Kunz,H.-H.
TITLE Direct Submission
JOURNAL Submitted (17-AUG-2020) School of Biological Sciences, Washington
State University, 1150 NE College Ave #385, Pullman, WA 99163, USA
COMMENT ##Assembly-Data-START##
Sequencing Technology :: Sanger dideoxy sequencing
##Assembly-Data-END##
FEATURES Location/Qualifiers
source 1..5545
/organism="Cloning vector pG20_FLAG_Hyg"
/mol_type="other DNA"
/db_xref="taxon:2782034"
regulatory 30..375
/regulatory_class="promoter"
/note="CaMV 35S"
CDS 395..1420
/note="confers resistance to hygromycin"
/codon_start=1
/transl_table=11
/product="hygromycin B phosphotransferase"
/protein_id="QOU11667.1"
/translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQD
LPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVY
HWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWS
EAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQ
SLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPS
TRPRAKE"
regulatory 1652..1828
/regulatory_class="other"
/note="CaMV poly(A)"
repeat_region 1836..1858
/note="TDNA LB"
rep_origin 1985..2420
/note="pSa"
CDS 2711..3526
/note="confers resistance to kanamycin"
/codon_start=1
/transl_table=11
/product="aminoglycoside phosphotransferase"
/protein_id="QOU11668.1"
/translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLY
GKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIP
GKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGL
VDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVG
RVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
repeat_region 3559..3583
/note="TDNA RB"
rep_origin 3697..4285
/note="ColE1"
repeat_region 4376..4400
/note="TDNA RB"
regulatory complement(4565..4814)
/regulatory_class="terminator"
/note="HSP18.2"
misc_feature complement(4824..4847)
/note="FLAG tag"
misc_feature complement(4848..4925)
/note="MCS"
regulatory complement(4932..5545)
/regulatory_class="promoter"
/note="pUBQ10"
BASE COUNT 1518 a 1265 c 1342 g 1420 t
ORIGIN
1 cgtttattac tgactcgtcg aagctttatt gagacttttc aacaaagggt aatttcggga
61 aacctcctcg gattccattg cccagctatc tgtcacttca tcgaaaggac agtagaaaag
121 gaaggtggct cctacaaatg ccatcattgc gataaaggaa aggctatcat tcaagatgcc
181 tctgccgaca gtggtcccaa agatggaccc ccacccacga ggagcatcgt ggaaaaagaa
241 gtcgttccaa ccacgacttc aaagcaagtg gattgatgtg acatctccac tgacgtaagg
301 gatgacgcac aatcccacta tccttcgcaa gacccttcct ctatataagg aagttcattt
361 catttggaga ggacagccca agctgatccc ccctatgaaa aagcctgaac tcaccgcgac
421 gtctgtcgag aagtttctga tcgaaaagtt cgacagcgtc tccgacctga tgcagctctc
481 ggagggcgaa gaatctcgtg ctttcagctt cgatgtagga gggcgtggat atgtcctgcg
541 ggtaaatagc tgcgccgatg gtttctacaa agatcgttat gtttatcggc actttgcatc
601 ggccgcgctc ccgattccgg aagtgcttga cattggggag ttcagcgaga gcctgaccta
661 ttgcatctcc cgccgtgcac agggtgtcac gttgcaagac ctgcctgaaa ccgaactgcc
721 cgctgttctt cagccggtcg cggaggctat ggatgctatc gctgcggccg atcttagcca
781 gacgagcggg ttcggcccat tcggaccgca aggaatcggt caatacacta catggcgtga
841 tttcatatgc gcgattgctg atccccatgt gtatcactgg caaactgtga tggacgacac
901 cgtcagtgcg tccgtcgcgc aggctctcga tgagctgatg ctttgggccg aggactgccc
961 cgaagtccgg cacctcgtgc acgcggattt cggctccaac aatgtcctga cggacaatgg
1021 ccgcataaca gcggtcattg actggagcga ggcgatgttc ggggattccc aatacgaggt
1081 cgccaacatc ttcttctgga ggccgtggtt ggcttgtatg gagcagcaga cgcgctactt
1141 cgagcggagg catccggagc ttgcaggatc gccaaggctc cgggcgtata tgctccgcat
1201 tggtcttgac caactctatc agagcttggt tgacggcaat ttcgatgatg cagcttgggc
1261 gcagggtcga tgcgacgcaa tcgtccgatc cggagccggg actgtcgggc gtacacaaat
1321 cgcccgcaga agcgcggccg tctggaccga tggctgtgta gaagtactcg ccgatagtgg
1381 aaaccgacgc cccagcactc gtccgagggc aaaggaatag agtagatgcc gaccgaacaa
1441 gagctgattt cgagaacgcc tcagccagca actcgcgcga gcctagcaag gcaaatgcga
1501 gagaacggcc ttacgcttgg tggcacagtt ctcgtccaca gttcgctaag ctcgctcggc
1561 tgggtcgcgg gagggccggt cgcagtgatt caggaattaa ttcggtacgc tgaaatcacc
1621 agtctctctc tacaaatcta tctctctcta ttttctccat aaataatgtg tgagtagttt
1681 cccgataagg gaaattaggg ttcttatagg gtttcgctca tgtgttgagc atataagaaa
1741 cccttagtat gtatttgtat ttgtaaaata cttctatcaa taaaatttct aattcctaaa
1801 accaaaatcc agtactaaaa tccagatcga taacgttaca ccacaatata tcctgccaag
1861 atctaattcc ggggatcgga aatccagaag cccgagaggt tgccgccttt cgggcttttt
1921 ctttttcaaa aaaaaaaatt tataaaacga tctgttgcgg ccggccgccg ggttgtgggc
1981 aaaggcgctc gacggtgggc aaccgcttgc ggttgtccac gggcggagcc ggtgcgcgta
2041 gcgcattgtc cacaagccaa gggcgaccaa taattgatat atatattcat aattgaaaag
2101 ctaattgaac atactacttg ctgtaactac ttgccggagc gaggggtgtt tgcaagctgt
2161 tgatctgaaa gggctattag cgttctcacg tgcctttttg attagcgatt tcacgtgacc
2221 ttattagcga tttcacgtac tccgattagc gatttcacgt accctgatta gcgatttcac
2281 gtggatagtt tttggagcgg gccggaaagc cccgtgaatc aaggctttgc ggggcattag
2341 cggtttcacg tggataacta ccctctatcc acaggcttcc ggggataaaa aagcccgctc
2401 gacggcgggc tgttggatgg ggatcgcctg aatcgcccca tcatccagcc agaaagtgag
2461 ggagccacgg ttgatgagag ctttgttgta ggtggaccag ttggtgattt tgaacttttg
2521 ctttgccacg gaacggtctg cgttgtcggg aagatgcgtg atctgatcct tcaactcagc
2581 aaaagttcga tttattcaac aaagccacgt tgtgtctcaa aatctctgat gttacattgc
2641 acaagataaa aatatatcat catgaacaat aaaactgtct gcttacataa acagtaatac
2701 aaggggtgtt atgagccata ttcaacggga aacgtcttgc tcaaggccgc gattaaattc
2761 caacatggat gctgatttat atgggtataa atgggctcgc gataatgtcg ggcaatcagg
2821 tgcgacaatc taccgattgt atgggaagcc cgatgcgcca gagttgtttc tgaaacatgg
2881 caaaggtagc gttgccaatg atgttacaga tgagatggtc agactaaact ggctgacgga
2941 atttatgcct cttccgacca tcaagcattt tatccgtact cctgatgatg catggttact
3001 caccactgcg atcccaggga aaacagcatt ccaggtatta gaagaatatc ctgattcagg
3061 tgaaaatatt gttgatgcgc tggcagtgtt cctgcgccgg ttgcattcga ttcctgtttg
3121 taattgtcct tttaacagcg atcgcgtatt tcgtctcgct caggcgcaat cacgaatgaa
3181 taacggtttg gttgatgcga gtgattttga tgacgagcgt aatggctggc ctgttgaaca
3241 agtctggaaa gaaatgcata aacttttgcc attctcaccg gattcagtcg tcactcatgg
3301 tgatttctca cttgataacc ttatttttga cgaggggaaa ttaataggtt gtattgatgt
3361 tggacgagtc ggaatcgcag accgatacca ggatcttgcc atcctatgga actgcctcgg
3421 tgagttttct ccttcattac agaaacggct ttttcaaaaa tatggtattg ataatcctga
3481 tatgaataaa ttgcagtttc atttgatgct cgatgagttt ttctaatcac tagaccaatg
3541 ttacacatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
3601 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
3661 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
3721 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
3781 agagctacca actcttcttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
3841 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
3901 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
3961 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
4021 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
4081 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
4141 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
4201 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
4261 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
4321 cttttgctgg ccttttgctc acatgagatc tcaaacaaac acatacagcg acttagttta
4381 cccgccaata tatcctgtca aacactgata gtttaaactg aaggcgggaa acgacaatct
4441 gatcatgagc ggagaattaa gggagtcacg ttatgacccc cgccgatgac gcgggacaag
4501 ccgttttacg tttggaactg acagaaccgc aacgttgaag gagccactca gccgatctga
4561 attccttatc tttaatcata ttccatagtc cataccatag cacatacagt agttatatgc
4621 tgcagaagag atccaacaaa acattcacaa tggattatag aaacatttgt ttattcatta
4681 taatgagatc ttacattcat ttaatattag aaaaagccac aaattcataa cacaacaagc
4741 caagaaaaaa acacaaactt aagcacacaa gctatttatt tgacacacca aatatttcat
4801 cttcatcttc atatgagctc ttacttgtca tcgtcatcct tgtagtcccc gggagcggta
4861 ccctcgaggt cgacagtact atcgatggat ccactagtgg cgcgccaggc ctgagcccgg
4921 ttaactctag tctgttaatc agaaaaactc agattaatcg acaaattcga tcgcacaaac
4981 tagaaactaa caccagatct agatagaaat cacaaatcga agagtaatta ttcgacaaaa
5041 ctcaaattat ttgaacaaat cggatgatat ctatgaaacc ctaatcgaga attaagatga
5101 tatctaacga tcaaacccag aaaatcgact tcgatctaag attaacagaa tctaaaccaa
5161 agaacatata cgaaattggg atcgaacgaa aacaaaatcg aagattttga gagaataagg
5221 aacacagaaa tttaccttga tcacggtaga gagaattgag agaaagtttt taagattttg
5281 agaaattgaa atctgaattg tgaagaagaa gaggtctttg ggtattgttt tatagaagaa
5341 gaagaagaaa agacgaggac gactaggtca cgagaaagct aaggcggtga agcaatagct
5401 aataataaaa tgacacgtgt attgagcgtt gtttacacgc aaagttgttt ttggctaatt
5461 gccttatttt taggttgagg aaaagtattt gtgctttgag ttgataaaca cgactcgtgt
5521 gtgccggctg caaccacttt gacgc
//