LOCUS MT896413 5065 bp DNA circular SYN 03-NOV-2020
DEFINITION Cloning vector pG20_6xHis_Blp, complete sequence.
ACCESSION MT896413
VERSION MT896413.1
KEYWORDS .
SOURCE Cloning vector pG20_6xHis_Blp
ORGANISM Cloning vector pG20_6xHis_Blp
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 5065)
AUTHORS Pratt,A.I., Knoblauch,J. and Kunz,H.-H.
TITLE An updated pGREEN-based vector suite for cost-effective cloning in
plant molecular biology
JOURNAL MicroPubl Biol (2020)
REMARK Publication Status: Online-Only
DOI: 10.17912/micropub.biology.000317
REFERENCE 2 (bases 1 to 5065)
AUTHORS Pratt,A.I. and Kunz,H.-H.
TITLE Direct Submission
JOURNAL Submitted (17-AUG-2020) School of Biological Sciences, Washington
State University, 1150 NE College Ave #385, Pullman, WA 99163, USA
COMMENT ##Assembly-Data-START##
Sequencing Technology :: Sanger dideoxy sequencing
##Assembly-Data-END##
FEATURES Location/Qualifiers
source 1..5065
/organism="Cloning vector pG20_6xHis_Blp"
/mol_type="other DNA"
/db_xref="taxon:2782031"
regulatory 30..375
/regulatory_class="promoter"
/note="CaMV 35S"
CDS 395..946
/note="confers phosphinothricin resistance"
/codon_start=1
/transl_table=11
/product="phosphinothricin acetyltransferase"
/protein_id="QOU11665.1"
/translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQ
EWTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGL
GSTLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHD
VGFWQLDFSLPVPPRPVLPVTEI"
regulatory 1178..1354
/regulatory_class="other"
/note="CaMV poly(A)"
repeat_region 1362..1384
/note="TDNA LB"
rep_origin 1511..1946
/note="pSa"
CDS 2237..3052
/note="confers kanamycin resistance"
/codon_start=1
/transl_table=11
/product="aminoglycoside phosphotransferase"
/protein_id="QOU11666.1"
/translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLY
GKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIP
GKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGL
VDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVG
RVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
rep_origin 3223..3811
/note="ColE1"
repeat_region 3559..3583
/note="TDNA RB"
repeat_region 3902..3926
/note="TDNA RB"
regulatory complement(4091..4340)
/regulatory_class="terminator"
/note="HSP18.2"
misc_feature complement(4350..4367)
/note="His tag"
misc_feature complement(4368..4445)
/note="MCS"
regulatory complement(4452..5065)
/regulatory_class="promoter"
/note="pUBQ10"
BASE COUNT 1402 a 1178 c 1211 g 1274 t
ORIGIN
1 cgtttattac tgactcgtcg aagctttatt gagacttttc aacaaagggt aatttcggga
61 aacctcctcg gattccattg cccagctatc tgtcacttca tcgaaaggac agtagaaaag
121 gaaggtggct cctacaaatg ccatcattgc gataaaggaa aggctatcat tcaagatgcc
181 tctgccgaca gtggtcccaa agatggaccc ccacccacga ggagcatcgt ggaaaaagaa
241 gtcgttccaa ccacgacttc aaagcaagtg gattgatgtg acatctccac tgacgtaagg
301 gatgacgcac aatcccacta tccttcgcaa gacccttcct ctatataagg aagttcattt
361 catttggaga ggacagccca agctgatccc ccctatgagc ccagaacgac gcccggccga
421 catccgccgt gccaccgagg cggacatgcc ggcggtctgc accatcgtca accactacat
481 cgagacaagc acggtcaact tccgtaccga gccgcaggaa ccgcaggagt ggacggacga
541 cctcgtccgt ctgcgggagc gctatccctg gctcgtcgcc gaggtggacg gcgaggtcgc
601 cggcatcgcc tacgcgggtc cctggaaggc acgcaacgcc tacgactgga cggccgagtc
661 caccgtgtac gtctcccccc gccaccagcg gacgggactg ggctccacgc tctacaccca
721 cctgctgaag tccctggagg cacagggctt caagagcgtg gtcgctgtca tcgggctgcc
781 caacgacccg agcgtgcgca tgcacgaggc gctcggatat gccccccgcg gcatgctgcg
841 ggcggccggc ttcaagcacg ggaactggca tgacgtgggt ttctggcagc tggacttcag
901 cctgccggtg ccgccccgtc cggtcctgcc cgtcaccgaa atctgaagta gatgccgacc
961 gaacaagagc tgatttcgag aacgcctcag ccagcaactc gcgcgagcct agcaaggcaa
1021 atgcgagaga acggccttac gcttggtggc acagttctcg tccacagttc gctaagctcg
1081 ctcggctggg tcgcgggagg gccggtcgca gtgattcagg aattaattcg gtacgctgaa
1141 atcaccagtc tctctctaca aatctatctc tctctatttt ctccataaat aatgtgtgag
1201 tagtttcccg ataagggaaa ttagggttct tatagggttt cgctcatgtg ttgagcatat
1261 aagaaaccct tagtatgtat ttgtatttgt aaaatacttc tatcaataaa atttctaatt
1321 cctaaaacca aaatccagta ctaaaatcca gatcgataac gttacaccac aatatatcct
1381 gccaagatct aattccgggg atcggaaatc cagaagcccg agaggttgcc gcctttcggg
1441 ctttttcttt ttcaaaaaaa aaaatttata aaacgatctg ttgcggccgg ccgccgggtt
1501 gtgggcaaag gcgctcgacg gtgggcaacc gcttgcggtt gtccacgggc ggagccggtg
1561 cgcgtagcgc attgtccaca agccaagggc gaccaataat tgatatatat attcataatt
1621 gaaaagctaa ttgaacatac tacttgctgt aactacttgc cggagcgagg ggtgtttgca
1681 agctgttgat ctgaaagggc tattagcgtt ctcacgtgcc tttttgatta gcgatttcac
1741 gtgaccttat tagcgatttc acgtactccg attagcgatt tcacgtaccc tgattagcga
1801 tttcacgtgg atagtttttg gagcgggccg gaaagccccg tgaatcaagg ctttgcgggg
1861 cattagcggt ttcacgtgga taactaccct ctatccacag gcttccgggg ataaaaaagc
1921 ccgctcgacg gcgggctgtt ggatggggat cgcctgaatc gccccatcat ccagccagaa
1981 agtgagggag ccacggttga tgagagcttt gttgtaggtg gaccagttgg tgattttgaa
2041 cttttgcttt gccacggaac ggtctgcgtt gtcgggaaga tgcgtgatct gatccttcaa
2101 ctcagcaaaa gttcgattta ttcaacaaag ccacgttgtg tctcaaaatc tctgatgtta
2161 cattgcacaa gataaaaata tatcatcatg aacaataaaa ctgtctgctt acataaacag
2221 taatacaagg ggtgttatga gccatattca acgggaaacg tcttgctcaa ggccgcgatt
2281 aaattccaac atggatgctg atttatatgg gtataaatgg gctcgcgata atgtcgggca
2341 atcaggtgcg acaatctacc gattgtatgg gaagcccgat gcgccagagt tgtttctgaa
2401 acatggcaaa ggtagcgttg ccaatgatgt tacagatgag atggtcagac taaactggct
2461 gacggaattt atgcctcttc cgaccatcaa gcattttatc cgtactcctg atgatgcatg
2521 gttactcacc actgcgatcc cagggaaaac agcattccag gtattagaag aatatcctga
2581 ttcaggtgaa aatattgttg atgcgctggc agtgttcctg cgccggttgc attcgattcc
2641 tgtttgtaat tgtcctttta acagcgatcg cgtatttcgt ctcgctcagg cgcaatcacg
2701 aatgaataac ggtttggttg atgcgagtga ttttgatgac gagcgtaatg gctggcctgt
2761 tgaacaagtc tggaaagaaa tgcataaact tttgccattc tcaccggatt cagtcgtcac
2821 tcatggtgat ttctcacttg ataaccttat ttttgacgag gggaaattaa taggttgtat
2881 tgatgttgga cgagtcggaa tcgcagaccg ataccaggat cttgccatcc tatggaactg
2941 cctcggtgag ttttctcctt cattacagaa acggcttttt caaaaatatg gtattgataa
3001 tcctgatatg aataaattgc agtttcattt gatgctcgat gagtttttct aatcactaga
3061 ccaatgttac acatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
3121 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
3181 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
3241 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
3301 ggatcaagag ctaccaactc ttcttccgaa ggtaactggc ttcagcagag cgcagatacc
3361 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
3421 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
3481 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
3541 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
3601 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
3661 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
3721 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
3781 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
3841 cctggccttt tgctggcctt ttgctcacat gagatctcaa acaaacacat acagcgactt
3901 agtttacccg ccaatatatc ctgtcaaaca ctgatagttt aaactgaagg cgggaaacga
3961 caatctgatc atgagcggag aattaaggga gtcacgttat gacccccgcc gatgacgcgg
4021 gacaagccgt tttacgtttg gaactgacag aaccgcaacg ttgaaggagc cactcagccg
4081 atctgaattc cttatcttta atcatattcc atagtccata ccatagcaca tacagtagtt
4141 atatgctgca gaagagatcc aacaaaacat tcacaatgga ttatagaaac atttgtttat
4201 tcattataat gagatcttac attcatttaa tattagaaaa agccacaaat tcataacaca
4261 acaagccaag aaaaaaacac aaacttaagc acacaagcta tttatttgac acaccaaata
4321 tttcatcttc atcttcatat gagctcttag tgatggtgat ggtgatgccc gggagcggta
4381 ccctcgaggt cgacagtact atcgatggat ccactagtgg cgcgccaggc ctgagcccgg
4441 ttaactctag tctgttaatc agaaaaactc agattaatcg acaaattcga tcgcacaaac
4501 tagaaactaa caccagatct agatagaaat cacaaatcga agagtaatta ttcgacaaaa
4561 ctcaaattat ttgaacaaat cggatgatat ctatgaaacc ctaatcgaga attaagatga
4621 tatctaacga tcaaacccag aaaatcgact tcgatctaag attaacagaa tctaaaccaa
4681 agaacatata cgaaattggg atcgaacgaa aacaaaatcg aagattttga gagaataagg
4741 aacacagaaa tttaccttga tcacggtaga gagaattgag agaaagtttt taagattttg
4801 agaaattgaa atctgaattg tgaagaagaa gaggtctttg ggtattgttt tatagaagaa
4861 gaagaagaaa agacgaggac gactaggtca cgagaaagct aaggcggtga agcaatagct
4921 aataataaaa tgacacgtgt attgagcgtt gtttacacgc aaagttgttt ttggctaatt
4981 gccttatttt taggttgagg aaaagtattt gtgctttgag ttgataaaca cgactcgtgt
5041 gtgccggctg caaccacttt gacgc
//