LOCUS MT896410 5476 bp DNA circular SYN 03-NOV-2020
DEFINITION Cloning vector pG20_MCS_Hyg, complete sequence.
ACCESSION MT896410
VERSION MT896410.1
KEYWORDS .
SOURCE Cloning vector pG20_MCS_Hyg
ORGANISM Cloning vector pG20_MCS_Hyg
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 5476)
AUTHORS Pratt,A.I., Knoblauch,J. and Kunz,H.-H.
TITLE An updated pGREEN-based vector suite for cost-effective cloning in
plant molecular biology
JOURNAL MicroPubl Biol (2020)
REMARK Publication Status: Online-Only
DOI: 10.17912/micropub.biology.000317
REFERENCE 2 (bases 1 to 5476)
AUTHORS Pratt,A.I. and Kunz,H.-H.
TITLE Direct Submission
JOURNAL Submitted (17-AUG-2020) School of Biological Sciences, Washington
State University, 1150 NE College Ave #385, Pullman, WA 99163, USA
COMMENT ##Assembly-Data-START##
Sequencing Technology :: Sanger dideoxy sequencing
##Assembly-Data-END##
FEATURES Location/Qualifiers
source 1..5476
/organism="Cloning vector pG20_MCS_Hyg"
/mol_type="other DNA"
/db_xref="taxon:2782039"
rep_origin 63..498
/note="pSa"
repeat_region 625..647
/note="TDNA LB"
regulatory complement(655..831)
/regulatory_class="other"
/note="CaMV poly(A)"
CDS complement(1063..2088)
/note="confers hygromycin resistance"
/codon_start=1
/transl_table=11
/product="hygromycin B phosphotransferase"
/protein_id="QOU11659.1"
/translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQD
LPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVY
HWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWS
EAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQ
SLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPS
TRPRAKE"
regulatory complement(2108..2453)
/regulatory_class="promoter"
/note="CaMV 35S"
regulatory 2463..3096
/regulatory_class="promoter"
/note="pUBQ10"
misc_feature 3103..3180
/note="MCS"
regulatory 3145..3394
/regulatory_class="terminator"
/note="HSP18.2"
repeat_region 3559..3583
/note="TDNA RB"
rep_origin complement(3674..4262)
/note="ColE1"
CDS complement(4433..5248)
/note="confers kanamycin resistance"
/codon_start=1
/transl_table=11
/product="aminoglycoside phosphotransferase"
/protein_id="QOU11660.1"
/translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLY
GKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIP
GKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGL
VDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVG
RVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
BASE COUNT 1399 a 1325 c 1246 g 1506 t
ORIGIN
1 agctctcatc aaccgtggct ccctcacttt ctggctggat gatggggcga ttcaggcgat
61 ccccatccaa cagcccgccg tcgagcgggc ttttttatcc ccggaagcct gtggatagag
121 ggtagttatc cacgtgaaac cgctaatgcc ccgcaaagcc ttgattcacg gggctttccg
181 gcccgctcca aaaactatcc acgtgaaatc gctaatcagg gtacgtgaaa tcgctaatcg
241 gagtacgtga aatcgctaat aaggtcacgt gaaatcgcta atcaaaaagg cacgtgagaa
301 cgctaatagc cctttcagat caacagcttg caaacacccc tcgctccggc aagtagttac
361 agcaagtagt atgttcaatt agcttttcaa ttatgaatat atatatcaat tattggtcgc
421 ccttggcttg tggacaatgc gctacgcgca ccggctccgc ccgtggacaa ccgcaagcgg
481 ttgcccaccg tcgagcgcct ttgcccacaa cccggcggcc ggccgcaaca gatcgtttta
541 taaatttttt tttttgaaaa agaaaaagcc cgaaaggcgg caacctctcg ggcttctgga
601 tttccgatcc ccggaattag atcttggcag gatatattgt ggtgtaacgt tatcgatctg
661 gattttagta ctggattttg gttttaggaa ttagaaattt tattgataga agtattttac
721 aaatacaaat acatactaag ggtttcttat atgctcaaca catgagcgaa accctataag
781 aaccctaatt tcccttatcg ggaaactact cacacattat ttatggagaa aatagagaga
841 gatagatttg tagagagaga ctggtgattt cagcgtaccg aattaattcc tgaatcactg
901 cgaccggccc tcccgcgacc cagccgagcg agcttagcga actgtggacg agaactgtgc
961 caccaagcgt aaggccgttc tctcgcattt gccttgctag gctcgcgcga gttgctggct
1021 gaggcgttct cgaaatcagc tcttgttcgg tcggcatcta ctctattcct ttgccctcgg
1081 acgagtgctg gggcgtcggt ttccactatc ggcgagtact tctacacagc catcggtcca
1141 gacggccgcg cttctgcggg cgatttgtgt acgcccgaca gtcccggctc cggatcggac
1201 gattgcgtcg catcgaccct gcgcccaagc tgcatcatcg aaattgccgt caaccaagct
1261 ctgatagagt tggtcaagac caatgcggag catatacgcc cggagccttg gcgatcctgc
1321 aagctccgga tgcctccgct cgaagtagcg cgtctgctgc tccatacaag ccaaccacgg
1381 cctccagaag aagatgttgg cgacctcgta ttgggaatcc ccgaacatcg cctcgctcca
1441 gtcaatgacc gctgttatgc ggccattgtc cgtcaggaca ttgttggagc cgaaatccgc
1501 gtgcacgagg tgccggactt cggggcagtc ctcggcccaa agcatcagct catcgagagc
1561 ctgcgcgacg gacgcactga cggtgtcgtc catcacagtt tgccagtgat acacatgggg
1621 atcagcaatc gcgcatatga aatcacgcca tgtagtgtat tgaccgattc cttgcggtcc
1681 gaatgggccg aacccgctcg tctggctaag atcggccgca gcgatagcat ccatagcctc
1741 cgcgaccggc tgaagaacag cgggcagttc ggtttcaggc aggtcttgca acgtgacacc
1801 ctgtgcacgg cgggagatgc aataggtcag gctctcgctg aactccccaa tgtcaagcac
1861 ttccggaatc gggagcgcgg ccgatgcaaa gtgccgataa acataacgat ctttgtagaa
1921 accatcggcg cagctattta cccgcaggac atatccacgc cctcctacat cgaagctgaa
1981 agcacgagat tcttcgccct ccgagagctg catcaggtcg gagacgctgt cgaacttttc
2041 gatcagaaac ttctcgacag acgtcgcggt gagttcaggc tttttcatag gggggatcag
2101 cttgggctgt cctctccaaa tgaaatgaac ttccttatat agaggaaggg tcttgcgaag
2161 gatagtggga ttgtgcgtca tcccttacgt cagtggagat gtcacatcaa tccacttgct
2221 ttgaagtcgt ggttggaacg acttcttttt ccacgatgct cctcgtgggt gggggtccat
2281 ctttgggacc actgtcggca gaggcatctt gaatgatagc ctttccttta tcgcaatgat
2341 ggcatttgta ggagccacct tccttttcta ctgtcctttc gatgaagtga cagatagctg
2401 ggcaatggaa tccgaggagg tttcccgaaa ttaccctttg ttgaaaagtc tcaataaagc
2461 ttcgacgagt cagtaataaa cggcgtcaaa gtggttgcag ccggcacaca cgagtcgtgt
2521 ttatcaactc aaagcacaaa tacttttcct caacctaaaa ataaggcaat tagccaaaaa
2581 caactttgcg tgtaaacaac gctcaataca cgtgtcattt tattattagc tattgcttca
2641 ccgccttagc tttctcgtga cctagtcgtc ctcgtctttt cttcttcttc ttctataaaa
2701 caatacccaa agacctcttc ttcttcacaa ttcagatttc aatttctcaa aatcttaaaa
2761 actttctctc aattctctct accgtgatca aggtaaattt ctgtgttcct tattctctca
2821 aaatcttcga ttttgttttc gttcgatccc aatttcgtat atgttctttg gtttagattc
2881 tgttaatctt agatcgaagt cgattttctg ggtttgatcg ttagatatca tcttaattct
2941 cgattagggt ttcatagata tcatccgatt tgttcaaata atttgagttt tgtcgaataa
3001 ttactcttcg atttgtgatt tctatctaga tctggtgtta gtttctagtt tgtgcgatcg
3061 aatttgtcga ttaatctgag tttttctgat taacagacta gtggatccgt cgacctcgag
3121 ggtaccgctc ccgggtagga gctcatatga agatgaagat gaaatatttg gtgtgtcaaa
3181 taaatagctt gtgtgcttaa gtttgtgttt ttttcttggc ttgttgtgtt atgaatttgt
3241 ggctttttct aatattaaat gaatgtaaga tctcattata atgaataaac aaatgtttct
3301 ataatccatt gtgaatgttt tgttggatct cttctgcagc atataactac tgtatgtgct
3361 atggtatgga ctatggaata tgattaaaga taaggaattc agatcggctg agtggctcct
3421 tcaacgttgc ggttctgtca gttccaaacg taaaacggct tgtcccgcgt catcggcggg
3481 ggtcataacg tgactccctt aattctccgc tcatgatcag attgtcgttt cccgccttca
3541 gtttaaacta tcagtgtttg acaggatata ttggcgggta aactaagtcg ctgtatgtgt
3601 ttgtttgaga tctcatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
3661 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
3721 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
3781 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
3841 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
3901 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
3961 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
4021 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
4081 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
4141 tgaagccagt taccttcgga agaagagttg gtagctcttg atccggcaaa caaaccaccg
4201 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
4261 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
4321 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
4381 aatgaagttt taaatcaatc taaagtatat atgtgtaaca ttggtctagt gattagaaaa
4441 actcatcgag catcaaatga aactgcaatt tattcatatc aggattatca ataccatatt
4501 tttgaaaaag ccgtttctgt aatgaaggag aaaactcacc gaggcagttc cataggatgg
4561 caagatcctg gtatcggtct gcgattccga ctcgtccaac atcaatacaa cctattaatt
4621 tcccctcgtc aaaaataagg ttatcaagtg agaaatcacc atgagtgacg actgaatccg
4681 gtgagaatgg caaaagttta tgcatttctt tccagacttg ttcaacaggc cagccattac
4741 gctcgtcatc aaaatcactc gcatcaacca aaccgttatt cattcgtgat tgcgcctgag
4801 cgagacgaaa tacgcgatcg ctgttaaaag gacaattaca aacaggaatc gaatgcaacc
4861 ggcgcaggaa cactgccagc gcatcaacaa tattttcacc tgaatcagga tattcttcta
4921 atacctggaa tgctgttttc cctgggatcg cagtggtgag taaccatgca tcatcaggag
4981 tacggataaa atgcttgatg gtcggaagag gcataaattc cgtcagccag tttagtctga
5041 ccatctcatc tgtaacatca ttggcaacgc tacctttgcc atgtttcaga aacaactctg
5101 gcgcatcggg cttcccatac aatcggtaga ttgtcgcacc tgattgcccg acattatcgc
5161 gagcccattt atacccatat aaatcagcat ccatgttgga atttaatcgc ggccttgagc
5221 aagacgtttc ccgttgaata tggctcataa caccccttgt attactgttt atgtaagcag
5281 acagttttat tgttcatgat gatatatttt tatcttgtgc aatgtaacat cagagatttt
5341 gagacacaac gtggctttgt tgaataaatc gaacttttgc tgagttgaag gatcagatca
5401 cgcatcttcc cgacaacgca gaccgttccg tggcaaagca aaagttcaaa atcaccaact
5461 ggtccaccta caacaa
//