LOCUS MT896405 5764 bp DNA circular SYN 03-NOV-2020
DEFINITION Cloning vector pG20_mVenus_Blp, complete sequence.
ACCESSION MT896405
VERSION MT896405.1
KEYWORDS .
SOURCE Cloning vector pG20_mVenus_Blp
ORGANISM Cloning vector pG20_mVenus_Blp
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 5764)
AUTHORS Pratt,A.I., Knoblauch,J. and Kunz,H.-H.
TITLE An updated pGREEN-based vector suite for cost-effective cloning in
plant molecular biology
JOURNAL MicroPubl Biol (2020)
REMARK Publication Status: Online-Only
DOI: 10.17912/micropub.biology.000317
REFERENCE 2 (bases 1 to 5764)
AUTHORS Pratt,A.I. and Kunz,H.-H.
TITLE Direct Submission
JOURNAL Submitted (17-AUG-2020) School of Biological Sciences, Washington
State University, 1150 NE College Ave #385, Pullman, WA 99163, USA
COMMENT ##Assembly-Data-START##
Sequencing Technology :: Sanger dideoxy sequencing
##Assembly-Data-END##
FEATURES Location/Qualifiers
source 1..5764
/organism="Cloning vector pG20_mVenus_Blp"
/mol_type="other DNA"
/db_xref="taxon:2782147"
rep_origin 63..498
/note="pSa"
repeat_region 625..647
/note="TDNA LB"
regulatory complement(655..831)
/regulatory_class="other"
/note="CaMV poly(A)"
CDS complement(1063..1614)
/note="confers phosphinothricin resistance"
/codon_start=1
/transl_table=11
/product="phosphinothricin acetyltransferase"
/protein_id="QOU11644.1"
/translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQ
EWTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGL
GSTLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHD
VGFWQLDFSLPVPPRPVLPVTEI"
regulatory complement(1634..1979)
/regulatory_class="promoter"
/note="CaMV 35S"
regulatory 1989..2622
/regulatory_class="promoter"
/note="pUBQ10"
misc_feature 2623..2661
/note="MCS"
CDS 2707..3426
/codon_start=1
/transl_table=11
/product="yellow fluorescent protein"
/protein_id="QOU11645.1"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
LKLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFK
DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKN
GIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDH
MVLLEFVTAAGITLGMDELYK"
regulatory 3433..3682
/regulatory_class="terminator"
/note="HSP18.2"
repeat_region 3559..3583
/note="TDNA RB"
repeat_region 3847..3871
/note="TDNA RB"
rep_origin complement(3962..4550)
/note="ColE1"
CDS complement(4721..5536)
/note="confers kanamycin resistance"
/codon_start=1
/transl_table=11
/product="aminoglycoside phosphotransferase"
/protein_id="QOU11646.1"
/translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLY
GKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIP
GKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGL
VDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVG
RVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
BASE COUNT 1437 a 1446 c 1382 g 1499 t
ORIGIN
1 agctctcatc aaccgtggct ccctcacttt ctggctggat gatggggcga ttcaggcgat
61 ccccatccaa cagcccgccg tcgagcgggc ttttttatcc ccggaagcct gtggatagag
121 ggtagttatc cacgtgaaac cgctaatgcc ccgcaaagcc ttgattcacg gggctttccg
181 gcccgctcca aaaactatcc acgtgaaatc gctaatcagg gtacgtgaaa tcgctaatcg
241 gagtacgtga aatcgctaat aaggtcacgt gaaatcgcta atcaaaaagg cacgtgagaa
301 cgctaatagc cctttcagat caacagcttg caaacacccc tcgctccggc aagtagttac
361 agcaagtagt atgttcaatt agcttttcaa ttatgaatat atatatcaat tattggtcgc
421 ccttggcttg tggacaatgc gctacgcgca ccggctccgc ccgtggacaa ccgcaagcgg
481 ttgcccaccg tcgagcgcct ttgcccacaa cccggcggcc ggccgcaaca gatcgtttta
541 taaatttttt tttttgaaaa agaaaaagcc cgaaaggcgg caacctctcg ggcttctgga
601 tttccgatcc ccggaattag atcttggcag gatatattgt ggtgtaacgt tatcgatctg
661 gattttagta ctggattttg gttttaggaa ttagaaattt tattgataga agtattttac
721 aaatacaaat acatactaag ggtttcttat atgctcaaca catgagcgaa accctataag
781 aaccctaatt tcccttatcg ggaaactact cacacattat ttatggagaa aatagagaga
841 gatagatttg tagagagaga ctggtgattt cagcgtaccg aattaattcc tgaatcactg
901 cgaccggccc tcccgcgacc cagccgagcg agcttagcga actgtggacg agaactgtgc
961 caccaagcgt aaggccgttc tctcgcattt gccttgctag gctcgcgcga gttgctggct
1021 gaggcgttct cgaaatcagc tcttgttcgg tcggcatcta cttcagattt cggtgacggg
1081 caggaccgga cggggcggca ccggcaggct gaagtccagc tgccagaaac ccacgtcatg
1141 ccagttcccg tgcttgaagc cggccgcccg cagcatgccg cggggggcat atccgagcgc
1201 ctcgtgcatg cgcacgctcg ggtcgttggg cagcccgatg acagcgacca cgctcttgaa
1261 gccctgtgcc tccagggact tcagcaggtg ggtgtagagc gtggagccca gtcccgtccg
1321 ctggtggcgg ggggagacgt acacggtgga ctcggccgtc cagtcgtagg cgttgcgtgc
1381 cttccaggga cccgcgtagg cgatgccggc gacctcgccg tccacctcgg cgacgagcca
1441 gggatagcgc tcccgcagac ggacgaggtc gtccgtccac tcctgcggtt cctgcggctc
1501 ggtacggaag ttgaccgtgc ttgtctcgat gtagtggttg acgatggtgc agaccgccgg
1561 catgtccgcc tcggtggcac ggcggatgtc ggccgggcgt cgttctgggc tcataggggg
1621 gatcagcttg ggctgtcctc tccaaatgaa atgaacttcc ttatatagag gaagggtctt
1681 gcgaaggata gtgggattgt gcgtcatccc ttacgtcagt ggagatgtca catcaatcca
1741 cttgctttga agtcgtggtt ggaacgactt ctttttccac gatgctcctc gtgggtgggg
1801 gtccatcttt gggaccactg tcggcagagg catcttgaat gatagccttt cctttatcgc
1861 aatgatggca tttgtaggag ccaccttcct tttctactgt cctttcgatg aagtgacaga
1921 tagctgggca atggaatccg aggaggtttc ccgaaattac cctttgttga aaagtctcaa
1981 taaagcttcg acgagtcagt aataaacggc gtcaaagtgg ttgcagccgg cacacacgag
2041 tcgtgtttat caactcaaag cacaaatact tttcctcaac ctaaaaataa ggcaattagc
2101 caaaaacaac tttgcgtgta aacaacgctc aatacacgtg tcattttatt attagctatt
2161 gcttcaccgc cttagctttc tcgtgaccta gtcgtcctcg tcttttcttc ttcttcttct
2221 ataaaacaat acccaaagac ctcttcttct tcacaattca gatttcaatt tctcaaaatc
2281 ttaaaaactt tctctcaatt ctctctaccg tgatcaaggt aaatttctgt gttccttatt
2341 ctctcaaaat cttcgatttt gttttcgttc gatcccaatt tcgtatatgt tctttggttt
2401 agattctgtt aatcttagat cgaagtcgat tttctgggtt tgatcgttag atatcatctt
2461 aattctcgat tagggtttca tagatatcat ccgatttgtt caaataattt gagttttgtc
2521 gaataattac tcttcgattt gtgatttcta tctagatctg gtgttagttt ctagtttgtg
2581 cgatcgaatt tgtcgattaa tctgagtttt tctgattaac agactagagt taaccgggct
2641 caggcctggc gcgccactag tggatccatc gatagtactg tcgacctcga gggtaccgct
2701 cccgggatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag
2761 ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc
2821 acctacggca agctgaccct gaagctgatc tgcaccaccg gcaagctgcc cgtgccctgg
2881 cccaccctcg tgaccaccct gggctacggc ctgcagtgct tcgcccgcta ccccgaccac
2941 atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc
3001 atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac
3061 accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg
3121 gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcaccgc cgacaagcag
3181 aagaacggca tcaaggccaa cttcaagatc cgccacaaca tcgaggacgg cggcgtgcag
3241 ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac
3301 aaccactacc tgagctacca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac
3361 atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac
3421 aagtaagagc tcatatgaag atgaagatga aatatttggt gtgtcaaata aatagcttgt
3481 gtgcttaagt ttgtgttttt ttcttggctt gttgtgttat gaatttgtgg ctttttctaa
3541 tattaaatga atgtaagatc tcattataat gaataaacaa atgtttctat aatccattgt
3601 gaatgttttg ttggatctct tctgcagcat ataactactg tatgtgctat ggtatggact
3661 atggaatatg attaaagata aggaattcag atcggctgag tggctccttc aacgttgcgg
3721 ttctgtcagt tccaaacgta aaacggcttg tcccgcgtca tcggcggggg tcataacgtg
3781 actcccttaa ttctccgctc atgatcagat tgtcgtttcc cgccttcagt ttaaactatc
3841 agtgtttgac aggatatatt ggcgggtaaa ctaagtcgct gtatgtgttt gtttgagatc
3901 tcatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
3961 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
4021 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
4081 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
4141 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
4201 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
4261 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
4321 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
4381 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
4441 ccttcggaag aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
4501 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
4561 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
4621 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
4681 aatcaatcta aagtatatat gtgtaacatt ggtctagtga ttagaaaaac tcatcgagca
4741 tcaaatgaaa ctgcaattta ttcatatcag gattatcaat accatatttt tgaaaaagcc
4801 gtttctgtaa tgaaggagaa aactcaccga ggcagttcca taggatggca agatcctggt
4861 atcggtctgc gattccgact cgtccaacat caatacaacc tattaatttc ccctcgtcaa
4921 aaataaggtt atcaagtgag aaatcaccat gagtgacgac tgaatccggt gagaatggca
4981 aaagtttatg catttctttc cagacttgtt caacaggcca gccattacgc tcgtcatcaa
5041 aatcactcgc atcaaccaaa ccgttattca ttcgtgattg cgcctgagcg agacgaaata
5101 cgcgatcgct gttaaaagga caattacaaa caggaatcga atgcaaccgg cgcaggaaca
5161 ctgccagcgc atcaacaata ttttcacctg aatcaggata ttcttctaat acctggaatg
5221 ctgttttccc tgggatcgca gtggtgagta accatgcatc atcaggagta cggataaaat
5281 gcttgatggt cggaagaggc ataaattccg tcagccagtt tagtctgacc atctcatctg
5341 taacatcatt ggcaacgcta cctttgccat gtttcagaaa caactctggc gcatcgggct
5401 tcccatacaa tcggtagatt gtcgcacctg attgcccgac attatcgcga gcccatttat
5461 acccatataa atcagcatcc atgttggaat ttaatcgcgg ccttgagcaa gacgtttccc
5521 gttgaatatg gctcataaca ccccttgtat tactgtttat gtaagcagac agttttattg
5581 ttcatgatga tatattttta tcttgtgcaa tgtaacatca gagattttga gacacaacgt
5641 ggctttgttg aataaatcga acttttgctg agttgaagga tcagatcacg catcttcccg
5701 acaacgcaga ccgttccgtg gcaaagcaaa agttcaaaat caccaactgg tccacctaca
5761 acaa
//