LOCUS MT896403 5755 bp DNA circular SYN 03-NOV-2020
DEFINITION Cloning vector pG20_mCherry_Blp, complete sequence.
ACCESSION MT896403
VERSION MT896403.1
KEYWORDS .
SOURCE Cloning vector pG20_mCherry_Blp
ORGANISM Cloning vector pG20_mCherry_Blp
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 5755)
AUTHORS Pratt,A.I., Knoblauch,J. and Kunz,H.-H.
TITLE An updated pGREEN-based vector suite for cost-effective cloning in
plant molecular biology
JOURNAL MicroPubl Biol (2020)
REMARK Publication Status: Online-Only
DOI: 10.17912/micropub.biology.000317
REFERENCE 2 (bases 1 to 5755)
AUTHORS Pratt,A.I. and Kunz,H.-H.
TITLE Direct Submission
JOURNAL Submitted (17-AUG-2020) School of Biological Sciences, Washington
State University, 1150 NE College Ave #385, Pullman, WA 99163, USA
COMMENT ##Assembly-Data-START##
Sequencing Technology :: Sanger dideoxy sequencing
##Assembly-Data-END##
FEATURES Location/Qualifiers
source 1..5755
/organism="Cloning vector pG20_mCherry_Blp"
/mol_type="other DNA"
/db_xref="taxon:2782043"
rep_origin 63..498
/note="pSa"
repeat_region 625..647
/note="TDNA LB"
regulatory complement(655..831)
/regulatory_class="other"
/note="CaMV poly(A)"
CDS complement(1063..1614)
/note="confers phosphinothricin resistance"
/codon_start=1
/transl_table=11
/product="phosphinothricin acetyltransferase"
/protein_id="QOU11638.1"
/translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQ
EWTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGL
GSTLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHD
VGFWQLDFSLPVPPRPVLPVTEI"
regulatory complement(1634..1979)
/regulatory_class="promoter"
/note="CaMV 35S"
regulatory 1989..2622
/regulatory_class="promoter"
/note="pUBQ10"
misc_feature 2623..2661
/note="MCS"
CDS 2707..3417
/codon_start=1
/transl_table=11
/product="red fluorescent protein"
/protein_id="QOU11639.1"
/translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYE
GTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVM
NFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDG
ALKGEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQ
YERAEGRHSTGGMDELYK"
regulatory 3424..3673
/regulatory_class="terminator"
/note="HSP18.2"
repeat_region 3559..3583
/note="TDNA RB"
repeat_region 3838..3862
/note="TDNA RB"
rep_origin complement(3953..4541)
/note="ColE1"
CDS complement(4712..5527)
/note="confers kanamycin resistance"
/codon_start=1
/transl_table=11
/product="aminoglycoside phosphotransferase"
/protein_id="QOU11640.1"
/translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLY
GKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIP
GKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGL
VDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVG
RVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
BASE COUNT 1430 a 1427 c 1399 g 1499 t
ORIGIN
1 agctctcatc aaccgtggct ccctcacttt ctggctggat gatggggcga ttcaggcgat
61 ccccatccaa cagcccgccg tcgagcgggc ttttttatcc ccggaagcct gtggatagag
121 ggtagttatc cacgtgaaac cgctaatgcc ccgcaaagcc ttgattcacg gggctttccg
181 gcccgctcca aaaactatcc acgtgaaatc gctaatcagg gtacgtgaaa tcgctaatcg
241 gagtacgtga aatcgctaat aaggtcacgt gaaatcgcta atcaaaaagg cacgtgagaa
301 cgctaatagc cctttcagat caacagcttg caaacacccc tcgctccggc aagtagttac
361 agcaagtagt atgttcaatt agcttttcaa ttatgaatat atatatcaat tattggtcgc
421 ccttggcttg tggacaatgc gctacgcgca ccggctccgc ccgtggacaa ccgcaagcgg
481 ttgcccaccg tcgagcgcct ttgcccacaa cccggcggcc ggccgcaaca gatcgtttta
541 taaatttttt tttttgaaaa agaaaaagcc cgaaaggcgg caacctctcg ggcttctgga
601 tttccgatcc ccggaattag atcttggcag gatatattgt ggtgtaacgt tatcgatctg
661 gattttagta ctggattttg gttttaggaa ttagaaattt tattgataga agtattttac
721 aaatacaaat acatactaag ggtttcttat atgctcaaca catgagcgaa accctataag
781 aaccctaatt tcccttatcg ggaaactact cacacattat ttatggagaa aatagagaga
841 gatagatttg tagagagaga ctggtgattt cagcgtaccg aattaattcc tgaatcactg
901 cgaccggccc tcccgcgacc cagccgagcg agcttagcga actgtggacg agaactgtgc
961 caccaagcgt aaggccgttc tctcgcattt gccttgctag gctcgcgcga gttgctggct
1021 gaggcgttct cgaaatcagc tcttgttcgg tcggcatcta cttcagattt cggtgacggg
1081 caggaccgga cggggcggca ccggcaggct gaagtccagc tgccagaaac ccacgtcatg
1141 ccagttcccg tgcttgaagc cggccgcccg cagcatgccg cggggggcat atccgagcgc
1201 ctcgtgcatg cgcacgctcg ggtcgttggg cagcccgatg acagcgacca cgctcttgaa
1261 gccctgtgcc tccagggact tcagcaggtg ggtgtagagc gtggagccca gtcccgtccg
1321 ctggtggcgg ggggagacgt acacggtgga ctcggccgtc cagtcgtagg cgttgcgtgc
1381 cttccaggga cccgcgtagg cgatgccggc gacctcgccg tccacctcgg cgacgagcca
1441 gggatagcgc tcccgcagac ggacgaggtc gtccgtccac tcctgcggtt cctgcggctc
1501 ggtacggaag ttgaccgtgc ttgtctcgat gtagtggttg acgatggtgc agaccgccgg
1561 catgtccgcc tcggtggcac ggcggatgtc ggccgggcgt cgttctgggc tcataggggg
1621 gatcagcttg ggctgtcctc tccaaatgaa atgaacttcc ttatatagag gaagggtctt
1681 gcgaaggata gtgggattgt gcgtcatccc ttacgtcagt ggagatgtca catcaatcca
1741 cttgctttga agtcgtggtt ggaacgactt ctttttccac gatgctcctc gtgggtgggg
1801 gtccatcttt gggaccactg tcggcagagg catcttgaat gatagccttt cctttatcgc
1861 aatgatggca tttgtaggag ccaccttcct tttctactgt cctttcgatg aagtgacaga
1921 tagctgggca atggaatccg aggaggtttc ccgaaattac cctttgttga aaagtctcaa
1981 taaagcttcg acgagtcagt aataaacggc gtcaaagtgg ttgcagccgg cacacacgag
2041 tcgtgtttat caactcaaag cacaaatact tttcctcaac ctaaaaataa ggcaattagc
2101 caaaaacaac tttgcgtgta aacaacgctc aatacacgtg tcattttatt attagctatt
2161 gcttcaccgc cttagctttc tcgtgaccta gtcgtcctcg tcttttcttc ttcttcttct
2221 ataaaacaat acccaaagac ctcttcttct tcacaattca gatttcaatt tctcaaaatc
2281 ttaaaaactt tctctcaatt ctctctaccg tgatcaaggt aaatttctgt gttccttatt
2341 ctctcaaaat cttcgatttt gttttcgttc gatcccaatt tcgtatatgt tctttggttt
2401 agattctgtt aatcttagat cgaagtcgat tttctgggtt tgatcgttag atatcatctt
2461 aattctcgat tagggtttca tagatatcat ccgatttgtt caaataattt gagttttgtc
2521 gaataattac tcttcgattt gtgatttcta tctagatctg gtgttagttt ctagtttgtg
2581 cgatcgaatt tgtcgattaa tctgagtttt tctgattaac agactagagt taaccgggct
2641 caggcctggc gcgccactag tggatccatc gatagtactg tcgacctcga gggtaccgct
2701 cccgggatgg tgagcaaggg cgaggaggat aacatggcca tcatcaagga gttcatgcgc
2761 ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt tcgagatcga gggcgagggc
2821 gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa gggtggcccc
2881 ctgcccttcg cctgggacat cctgtcccct cagttcatgt acggctccaa ggcctacgtg
2941 aagcaccccg ccgacatccc cgactacttg aagctgtcct tccccgaggg cttcaagtgg
3001 gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg tgacccagga ctcctccctg
3061 caggacggcg agttcatcta caaggtgaag ctgcgcggca ccaacttccc ctccgacggc
3121 cccgtaatgc agaagaagac catgggctgg gaggcctcct ccgagcggat gtaccccgag
3181 gacggcgccc tgaagggcga gatcaagcag aggctgaagc tgaaggacgg cggccactac
3241 gacgctgagg tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac
3301 aacgtcaaca tcaagttgga catcacctcc cacaacgagg actacaccat cgtggaacag
3361 tacgaacgcg ccgagggccg ccactccacc ggcggcatgg acgagctgta caagtaggag
3421 ctcatatgaa gatgaagatg aaatatttgg tgtgtcaaat aaatagcttg tgtgcttaag
3481 tttgtgtttt tttcttggct tgttgtgtta tgaatttgtg gctttttcta atattaaatg
3541 aatgtaagat ctcattataa tgaataaaca aatgtttcta taatccattg tgaatgtttt
3601 gttggatctc ttctgcagca tataactact gtatgtgcta tggtatggac tatggaatat
3661 gattaaagat aaggaattca gatcggctga gtggctcctt caacgttgcg gttctgtcag
3721 ttccaaacgt aaaacggctt gtcccgcgtc atcggcgggg gtcataacgt gactccctta
3781 attctccgct catgatcaga ttgtcgtttc ccgccttcag tttaaactat cagtgtttga
3841 caggatatat tggcgggtaa actaagtcgc tgtatgtgtt tgtttgagat ctcatgtgag
3901 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
3961 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
4021 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
4081 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
4141 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
4201 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
4261 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
4321 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
4381 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
4441 gaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
4501 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
4561 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
4621 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
4681 aaagtatata tgtgtaacat tggtctagtg attagaaaaa ctcatcgagc atcaaatgaa
4741 actgcaattt attcatatca ggattatcaa taccatattt ttgaaaaagc cgtttctgta
4801 atgaaggaga aaactcaccg aggcagttcc ataggatggc aagatcctgg tatcggtctg
4861 cgattccgac tcgtccaaca tcaatacaac ctattaattt cccctcgtca aaaataaggt
4921 tatcaagtga gaaatcacca tgagtgacga ctgaatccgg tgagaatggc aaaagtttat
4981 gcatttcttt ccagacttgt tcaacaggcc agccattacg ctcgtcatca aaatcactcg
5041 catcaaccaa accgttattc attcgtgatt gcgcctgagc gagacgaaat acgcgatcgc
5101 tgttaaaagg acaattacaa acaggaatcg aatgcaaccg gcgcaggaac actgccagcg
5161 catcaacaat attttcacct gaatcaggat attcttctaa tacctggaat gctgttttcc
5221 ctgggatcgc agtggtgagt aaccatgcat catcaggagt acggataaaa tgcttgatgg
5281 tcggaagagg cataaattcc gtcagccagt ttagtctgac catctcatct gtaacatcat
5341 tggcaacgct acctttgcca tgtttcagaa acaactctgg cgcatcgggc ttcccataca
5401 atcggtagat tgtcgcacct gattgcccga cattatcgcg agcccattta tacccatata
5461 aatcagcatc catgttggaa tttaatcgcg gccttgagca agacgtttcc cgttgaatat
5521 ggctcataac accccttgta ttactgttta tgtaagcaga cagttttatt gttcatgatg
5581 atatattttt atcttgtgca atgtaacatc agagattttg agacacaacg tggctttgtt
5641 gaataaatcg aacttttgct gagttgaagg atcagatcac gcatcttccc gacaacgcag
5701 accgttccgt ggcaaagcaa aagttcaaaa tcaccaactg gtccacctac aacaa
//