LOCUS MT896402 6229 bp DNA circular SYN 03-NOV-2020
DEFINITION Cloning vector pG20_mCherry_Hyg, complete sequence.
ACCESSION MT896402
VERSION MT896402.1
KEYWORDS .
SOURCE Cloning vector pG20_mCherry_Hyg
ORGANISM Cloning vector pG20_mCherry_Hyg
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 6229)
AUTHORS Pratt,A.I., Knoblauch,J. and Kunz,H.-H.
TITLE An updated pGREEN-based vector suite for cost-effective cloning in
plant molecular biology
JOURNAL MicroPubl Biol (2020)
REMARK Publication Status: Online-Only
DOI: 10.17912/micropub.biology.000317
REFERENCE 2 (bases 1 to 6229)
AUTHORS Pratt,A.I. and Kunz,H.-H.
TITLE Direct Submission
JOURNAL Submitted (17-AUG-2020) School of Biological Sciences, Washington
State University, 1150 NE College Ave #385, Pullman, WA 99163, USA
COMMENT ##Assembly-Data-START##
Sequencing Technology :: Sanger dideoxy sequencing
##Assembly-Data-END##
FEATURES Location/Qualifiers
source 1..6229
/organism="Cloning vector pG20_mCherry_Hyg"
/mol_type="other DNA"
/db_xref="taxon:2782044"
rep_origin 63..498
/note="pSa"
repeat_region 625..647
/note="TDNA LB"
regulatory complement(655..831)
/regulatory_class="other"
/note="CaMV poly(A)"
CDS complement(1063..2088)
/note="confers hygromycin resistance"
/codon_start=1
/transl_table=11
/product="hygromycin B phosphotransferase"
/protein_id="QOU11635.1"
/translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQD
LPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVY
HWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWS
EAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQ
SLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPS
TRPRAKE"
regulatory complement(2108..2453)
/regulatory_class="promoter"
/note="CaMV 35S"
regulatory 2463..3096
/regulatory_class="promoter"
/note="pUBQ10"
misc_feature 3103..3180
/note="MCS"
CDS 3181..3891
/codon_start=1
/transl_table=11
/product="red fluorescent protein"
/protein_id="QOU11636.1"
/translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYE
GTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVM
NFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDG
ALKGEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQ
YERAEGRHSTGGMDELYK"
repeat_region 3559..3583
/note="TDNA RB"
regulatory 3898..4147
/regulatory_class="terminator"
/note="HSP18.2"
repeat_region 4312..4336
/note="TDNA RB"
rep_origin complement(4427..5015)
/note="ColE1"
CDS complement(5186..6001)
/note="confers kanamycin resistance"
/codon_start=1
/transl_table=11
/product="aminoglycoside phosphotransferase"
/protein_id="QOU11637.1"
/translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLY
GKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIP
GKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGL
VDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVG
RVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
BASE COUNT 1572 a 1563 c 1479 g 1615 t
ORIGIN
1 agctctcatc aaccgtggct ccctcacttt ctggctggat gatggggcga ttcaggcgat
61 ccccatccaa cagcccgccg tcgagcgggc ttttttatcc ccggaagcct gtggatagag
121 ggtagttatc cacgtgaaac cgctaatgcc ccgcaaagcc ttgattcacg gggctttccg
181 gcccgctcca aaaactatcc acgtgaaatc gctaatcagg gtacgtgaaa tcgctaatcg
241 gagtacgtga aatcgctaat aaggtcacgt gaaatcgcta atcaaaaagg cacgtgagaa
301 cgctaatagc cctttcagat caacagcttg caaacacccc tcgctccggc aagtagttac
361 agcaagtagt atgttcaatt agcttttcaa ttatgaatat atatatcaat tattggtcgc
421 ccttggcttg tggacaatgc gctacgcgca ccggctccgc ccgtggacaa ccgcaagcgg
481 ttgcccaccg tcgagcgcct ttgcccacaa cccggcggcc ggccgcaaca gatcgtttta
541 taaatttttt tttttgaaaa agaaaaagcc cgaaaggcgg caacctctcg ggcttctgga
601 tttccgatcc ccggaattag atcttggcag gatatattgt ggtgtaacgt tatcgatctg
661 gattttagta ctggattttg gttttaggaa ttagaaattt tattgataga agtattttac
721 aaatacaaat acatactaag ggtttcttat atgctcaaca catgagcgaa accctataag
781 aaccctaatt tcccttatcg ggaaactact cacacattat ttatggagaa aatagagaga
841 gatagatttg tagagagaga ctggtgattt cagcgtaccg aattaattcc tgaatcactg
901 cgaccggccc tcccgcgacc cagccgagcg agcttagcga actgtggacg agaactgtgc
961 caccaagcgt aaggccgttc tctcgcattt gccttgctag gctcgcgcga gttgctggct
1021 gaggcgttct cgaaatcagc tcttgttcgg tcggcatcta ctctattcct ttgccctcgg
1081 acgagtgctg gggcgtcggt ttccactatc ggcgagtact tctacacagc catcggtcca
1141 gacggccgcg cttctgcggg cgatttgtgt acgcccgaca gtcccggctc cggatcggac
1201 gattgcgtcg catcgaccct gcgcccaagc tgcatcatcg aaattgccgt caaccaagct
1261 ctgatagagt tggtcaagac caatgcggag catatacgcc cggagccttg gcgatcctgc
1321 aagctccgga tgcctccgct cgaagtagcg cgtctgctgc tccatacaag ccaaccacgg
1381 cctccagaag aagatgttgg cgacctcgta ttgggaatcc ccgaacatcg cctcgctcca
1441 gtcaatgacc gctgttatgc ggccattgtc cgtcaggaca ttgttggagc cgaaatccgc
1501 gtgcacgagg tgccggactt cggggcagtc ctcggcccaa agcatcagct catcgagagc
1561 ctgcgcgacg gacgcactga cggtgtcgtc catcacagtt tgccagtgat acacatgggg
1621 atcagcaatc gcgcatatga aatcacgcca tgtagtgtat tgaccgattc cttgcggtcc
1681 gaatgggccg aacccgctcg tctggctaag atcggccgca gcgatagcat ccatagcctc
1741 cgcgaccggc tgaagaacag cgggcagttc ggtttcaggc aggtcttgca acgtgacacc
1801 ctgtgcacgg cgggagatgc aataggtcag gctctcgctg aactccccaa tgtcaagcac
1861 ttccggaatc gggagcgcgg ccgatgcaaa gtgccgataa acataacgat ctttgtagaa
1921 accatcggcg cagctattta cccgcaggac atatccacgc cctcctacat cgaagctgaa
1981 agcacgagat tcttcgccct ccgagagctg catcaggtcg gagacgctgt cgaacttttc
2041 gatcagaaac ttctcgacag acgtcgcggt gagttcaggc tttttcatag gggggatcag
2101 cttgggctgt cctctccaaa tgaaatgaac ttccttatat agaggaaggg tcttgcgaag
2161 gatagtggga ttgtgcgtca tcccttacgt cagtggagat gtcacatcaa tccacttgct
2221 ttgaagtcgt ggttggaacg acttcttttt ccacgatgct cctcgtgggt gggggtccat
2281 ctttgggacc actgtcggca gaggcatctt gaatgatagc ctttccttta tcgcaatgat
2341 ggcatttgta ggagccacct tccttttcta ctgtcctttc gatgaagtga cagatagctg
2401 ggcaatggaa tccgaggagg tttcccgaaa ttaccctttg ttgaaaagtc tcaataaagc
2461 ttcgacgagt cagtaataaa cggcgtcaaa gtggttgcag ccggcacaca cgagtcgtgt
2521 ttatcaactc aaagcacaaa tacttttcct caacctaaaa ataaggcaat tagccaaaaa
2581 caactttgcg tgtaaacaac gctcaataca cgtgtcattt tattattagc tattgcttca
2641 ccgccttagc tttctcgtga cctagtcgtc ctcgtctttt cttcttcttc ttctataaaa
2701 caatacccaa agacctcttc ttcttcacaa ttcagatttc aatttctcaa aatcttaaaa
2761 actttctctc aattctctct accgtgatca aggtaaattt ctgtgttcct tattctctca
2821 aaatcttcga ttttgttttc gttcgatccc aatttcgtat atgttctttg gtttagattc
2881 tgttaatctt agatcgaagt cgattttctg ggtttgatcg ttagatatca tcttaattct
2941 cgattagggt ttcatagata tcatccgatt tgttcaaata atttgagttt tgtcgaataa
3001 ttactcttcg atttgtgatt tctatctaga tctggtgtta gtttctagtt tgtgcgatcg
3061 aatttgtcga ttaatctgag tttttctgat taacagacta gagttaaccg ggctcaggcc
3121 tggcgcgcca ctagtggatc catcgatagt actgtcgacc tcgagggtac cgctcccggg
3181 atggtgagca agggcgagga ggataacatg gccatcatca aggagttcat gcgcttcaag
3241 gtgcacatgg agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc
3301 cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc
3361 ttcgcctggg acatcctgtc ccctcagttc atgtacggct ccaaggccta cgtgaagcac
3421 cccgccgaca tccccgacta cttgaagctg tccttccccg agggcttcaa gtgggagcgc
3481 gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc aggactcctc cctgcaggac
3541 ggcgagttca tctacaaggt gaagctgcgc ggcaccaact tcccctccga cggccccgta
3601 atgcagaaga agaccatggg ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc
3661 gccctgaagg gcgagatcaa gcagaggctg aagctgaagg acggcggcca ctacgacgct
3721 gaggtcaaga ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc
3781 aacatcaagt tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgaa
3841 cgcgccgagg gccgccactc caccggcggc atggacgagc tgtacaagta ggagctcata
3901 tgaagatgaa gatgaaatat ttggtgtgtc aaataaatag cttgtgtgct taagtttgtg
3961 tttttttctt ggcttgttgt gttatgaatt tgtggctttt tctaatatta aatgaatgta
4021 agatctcatt ataatgaata aacaaatgtt tctataatcc attgtgaatg ttttgttgga
4081 tctcttctgc agcatataac tactgtatgt gctatggtat ggactatgga atatgattaa
4141 agataaggaa ttcagatcgg ctgagtggct ccttcaacgt tgcggttctg tcagttccaa
4201 acgtaaaacg gcttgtcccg cgtcatcggc gggggtcata acgtgactcc cttaattctc
4261 cgctcatgat cagattgtcg tttcccgcct tcagtttaaa ctatcagtgt ttgacaggat
4321 atattggcgg gtaaactaag tcgctgtatg tgtttgtttg agatctcatg tgagcaaaag
4381 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
4441 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
4501 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
4561 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
4621 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
4681 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
4741 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
4801 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
4861 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaagaagag
4921 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
4981 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
5041 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
5101 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
5161 tatatgtgta acattggtct agtgattaga aaaactcatc gagcatcaaa tgaaactgca
5221 atttattcat atcaggatta tcaataccat atttttgaaa aagccgtttc tgtaatgaag
5281 gagaaaactc accgaggcag ttccatagga tggcaagatc ctggtatcgg tctgcgattc
5341 cgactcgtcc aacatcaata caacctatta atttcccctc gtcaaaaata aggttatcaa
5401 gtgagaaatc accatgagtg acgactgaat ccggtgagaa tggcaaaagt ttatgcattt
5461 ctttccagac ttgttcaaca ggccagccat tacgctcgtc atcaaaatca ctcgcatcaa
5521 ccaaaccgtt attcattcgt gattgcgcct gagcgagacg aaatacgcga tcgctgttaa
5581 aaggacaatt acaaacagga atcgaatgca accggcgcag gaacactgcc agcgcatcaa
5641 caatattttc acctgaatca ggatattctt ctaatacctg gaatgctgtt ttccctggga
5701 tcgcagtggt gagtaaccat gcatcatcag gagtacggat aaaatgcttg atggtcggaa
5761 gaggcataaa ttccgtcagc cagtttagtc tgaccatctc atctgtaaca tcattggcaa
5821 cgctaccttt gccatgtttc agaaacaact ctggcgcatc gggcttccca tacaatcggt
5881 agattgtcgc acctgattgc ccgacattat cgcgagccca tttataccca tataaatcag
5941 catccatgtt ggaatttaat cgcggccttg agcaagacgt ttcccgttga atatggctca
6001 taacacccct tgtattactg tttatgtaag cagacagttt tattgttcat gatgatatat
6061 ttttatcttg tgcaatgtaa catcagagat tttgagacac aacgtggctt tgttgaataa
6121 atcgaacttt tgctgagttg aaggatcaga tcacgcatct tcccgacaac gcagaccgtt
6181 ccgtggcaaa gcaaaagttc aaaatcacca actggtccac ctacaacaa
//