LOCUS MK787259 1811 bp DNA linear SYN 11-MAY-2019
DEFINITION Synthetic construct clone TERG_08336 multidrug resistance protein
TruMFS2 gene, complete cds.
ACCESSION MK787259
VERSION MK787259.1
KEYWORDS TruMFS2.
SOURCE synthetic construct
ORGANISM synthetic construct
other sequences; artificial sequences.
REFERENCE 1 (bases 1 to 1811)
AUTHORS Monod,M., Feuermann,M., Alshahni,M.M. and Yamada,T.
TITLE Trichophyton rubrum azole resistance mediated by a new ABC
transporter TruMDR3
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 1811)
AUTHORS Monod,M., Feuermann,M., Alshahni,M.M. and Yamada,T.
TITLE Direct Submission
JOURNAL Submitted (11-APR-2019) Medical Mycology, Graduate School of
Medicine, Teikyo University, 2-11-1 Kaga, Itabashi, Tokyo 173-8605,
Japan
COMMENT ##Assembly-Data-START##
Sequencing Technology :: Sanger dideoxy sequencing
##Assembly-Data-END##
FEATURES Location/Qualifiers
source 1..1811
/organism="synthetic construct"
/mol_type="other DNA"
/db_xref="taxon:32630"
/clone="TERG_08336"
/note="derived from Trichophyton rubrum; cloned into pYES2
for gene expression"
misc_feature 1..12
/note="linker containing BamHI site"
CDS 13..1800
/codon_start=1
/transl_table=11
/product="multidrug resistance protein TruMFS2"
/protein_id="QCL08050.1"
/translation="MADANAMEGEKSIPTKIPHWRQVTDPGAVTPEIINYPYPGSGTE
ADPYLVQWIPSDPRNPMNYSAVKKWSITFVVAIATLAVALISSAYTGGAIEIAQEFHA
DAEVITLGVSLFVLGFAIGPLIWAPMSELFGRQLLFFGTYLALTAFNAGAAGSPNMAT
LLVLRFFAGSFGSSPLTNAGGVIADMFPASHRGLAMGIFAIAPFLGPVLGPVIGGFLG
ESAGWRWVEGFLAIFSGVVWIIGSIFLPETYPPVLLRKRAQRLSKLTGKVYASRMDIE
QGKLSIGQAFKTALMRPWILLFREPIVLLLSTYMAIVYGTLYMLFSAFPVVYQQHRGW
SPGIGGLAFLGVLGGILAAMVINLLDNKRYAKVSKEYNGFAPPEERLPVAIIGGIAIP
IGLFWFAWTNGPQIHWIVSIIASAPFGFGMVLVFLSLMNYLIDAYTIYAASVLAANSV
LRSLFGAAFPLFTRYMYQNLGIHWASTIPAFLALACVPFPFLFYIYGANIRKRCKFAG
EADAFMQKLMQANSAHLESDLEVSEAGPIPRRNSLEAREVLDRIQSARSGLTRTRTAA
TVEYEGNPYDIDRVNTGLSRVSTTNSQTR"
misc_feature 1801..1811
/note="linker containing NotI site"
BASE COUNT 418 a 443 c 433 g 517 t
ORIGIN
1 gttggatcca acatggctga tgccaatgcc atggagggag agaagtcgat accgaccaag
61 attcctcact ggagacaagt aactgatcca ggcgccgtca caccagagat aattaattat
121 ccctacccag gctccggaac ggaagcagac ccttatcttg tacaatggat tccaagcgat
181 cctcgaaacc cgatgaacta ctctgccgtg aaaaaatggt cgatcacctt cgttgttgcc
241 atagctacgc ttgccgtcgc cttgatatcg tctgcatata ccggaggagc aatcgaaatt
301 gctcaagaat ttcatgctga cgcggaagtt attacactgg gtgtctcctt gttcgtcctt
361 ggttttgcaa tcggcccact gatatgggct cctatgagcg aattatttgg ccgccaactg
421 ctattcttcg gcacctacct tgctttgaca gcctttaatg ctggggcagc tggtagccct
481 aatatggcaa cgttgcttgt tctccgattc tttgctggtt cttttggctc atcccctttg
541 actaatgccg ggggtgtaat tgcagatatg tttccggctt cacatcgtgg attggccatg
601 ggcatattcg ccatagcccc ttttttgggc ccagtactgg gtccagttat tggaggattt
661 cttggagagt cagcaggttg gagatgggtc gaaggattcc tggccatatt ctctggagtt
721 gtctggatta ttggttctat tttccttccg gaaacatatc cccctgtgct ccttcgcaag
781 agggctcagc gactgtctaa acttactgga aaggtatacg cgagcagaat ggatattgaa
841 caagggaaat tatccattgg acaagctttc aagactgcac tcatgcgacc ctggattctc
901 ttattccgcg aacccatcgt tttgttgctg tcaacatata tggcaattgt ctatggaaca
961 ctttacatgc tcttttcagc atttccggtc gtttatcagc aacatagggg ttggagcccc
1021 ggaatcggag ggcttgcatt cttgggagtc cttggcggaa ttctcgccgc tatggttatc
1081 aaccttctag ataacaaaag atatgcaaaa gtttcgaagg aatacaatgg cttcgcacct
1141 cctgaagaac ggctgccagt tgcgattatt gggggaattg ctattccgat cgggctcttc
1201 tggtttgctt ggactaatgg acctcagata cattggattg tatcaatcat agcatcggct
1261 ccgtttggct tcggcatggt ccttgttttc ttgagtctaa tgaattatct cattgatgca
1321 tatactatat atgctgcctc ggtccttgcc gcgaattctg tgcttcgatc tctgtttggc
1381 gcagcgttcc cattatttac ccgatatatg taccagaacc ttggaatcca ctgggcctca
1441 actattcccg ccttcctggc actggcgtgt gttccctttc ctttcttgtt ctacatatac
1501 ggcgcaaaca ttcgaaaacg ctgcaaattt gctggggagg cggatgcatt tatgcaaaaa
1561 cttatgcagg caaactcagc gcaccttgag agtgatttag aggtatcaga ggccggccct
1621 ataccgagaa gaaacagcct agaagcacga gaggtgctgg acagaataca atctgctcga
1681 tctggtctca cgcgtactcg tacagcagca accgtcgagt atgaagggaa cccctacgat
1741 attgatcgtg taaataccgg tctttcccgc gttagcacta caaattccca gacgcgttaa
1801 gcggccgctt g
//