LOCUS MK787257 2435 bp DNA linear SYN 11-MAY-2019
DEFINITION Synthetic construct clone TERG_06399 hypothetical protein gene,
complete cds.
ACCESSION MK787257
VERSION MK787257.1
KEYWORDS .
SOURCE synthetic construct
ORGANISM synthetic construct
other sequences; artificial sequences.
REFERENCE 1 (bases 1 to 2435)
AUTHORS Monod,M., Feuermann,M., Alshahni,M.M. and Yamada,T.
TITLE Trichophyton rubrum azole resistance mediated by a new ABC
transporter TruMDR3
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 2435)
AUTHORS Monod,M., Feuermann,M., Alshahni,M.M. and Yamada,T.
TITLE Direct Submission
JOURNAL Submitted (11-APR-2019) Medical Mycology, Graduate School of
Medicine, Teikyo University, 2-11-1 Kaga, Itabashi, Tokyo 173-8605,
Japan
COMMENT ##Assembly-Data-START##
Sequencing Technology :: Sanger dideoxy sequencing
##Assembly-Data-END##
FEATURES Location/Qualifiers
source 1..2435
/organism="synthetic construct"
/mol_type="other DNA"
/db_xref="taxon:32630"
/clone="TERG_06399"
/note="derived from Trichophyton rubrum; cloned into pYES2
for gene expression"
misc_feature 1..12
/note="linker containing BamHI site"
CDS 13..2424
/codon_start=1
/transl_table=11
/product="hypothetical protein"
/protein_id="QCL08048.1"
/translation="MRGSRVLQKASFSRDTVRCSLLGLNLSSRCLATDSRAPLVRPVA
FSRQYHAVSPPVFGGNRLHRCGIFTPTSCPDVLGKLPRNALFPQSSNLIYRQLRFVTG
KSSPGENGSSQLQESTASVVKEVAQAEEISQAFERSERASQAAQINLSARLAKDGLAS
GKKSGLREIWRLITIARPEAKVLSVAFLFLLISSSVAMSIPFSIGKILDIATSSNPNA
LKDFLGISLPVFYTALGGIILLGAAANYGRIIILRIVGERIVARLRAKLFRNTFVQDA
EFFDANRVGDLISRLSSDTIIVGKSITQNLSDGLRAAVSGAAGFGMMAYVSLKLSSVL
ALLFPPIALAAFFYGRAIRNLSRKIQKNVGTLTKIAEERLGNVKTSQSFAGEILEVHR
YNRQVKRIFELGKKESFISATFFSGTSLMGNATILALLYVGGGMVQSQAISIGELTSF
LMYTAYAGSSLFGLSSFYSELMKGAGAASRLFELQDRRPKISPTVGDKVTSARGPIRF
KNVAFSYPTRPAVTIFKDLDFEIPQGSNVAIVGPSGGGKSTIASLLLRFYSPTQGQVF
INGKDVATMNAKSLRRKIGIVAQEPVLFSGSIAENISYGKPDATRSQIIAAARKANCQ
FISDFPDGLDTNVGPKGAQLSGGQKQRIAIARALIKEPDILILDEATSALDAESETLV
NSALAALLRENNTTISIAHRLSTIKRSDTIIVLDSEGKVAEMGSYNDLNSRPDGAFTK
LMAWQLSGGEGIAAPVHPNKPISRGPPSETEHLQQLLQEGEDEEYDEAEPEDEPRSTD
EIEKK"
misc_feature 2425..2435
/note="linker containing NotI site"
BASE COUNT 604 a 617 c 597 g 617 t
ORIGIN
1 gttggatcca acatgcgcgg ctcaagagtc ctccagaagg ccagtttttc tagagacacc
61 gtacgctgca gtctcttggg cctcaatctc tcgtcacgat gtcttgcaac cgatagtagg
121 gcgccgttgg tacgtcccgt ggccttttcc aggcagtacc atgccgtttc gccgccggtg
181 tttggtggaa acagactcca tagatgcgga attttcaccc ctacctcttg tccagatgtc
241 ctcggaaagc tcccccgaaa cgccttgttc ccccaatcct cgaatctcat atacaggcag
301 ctccgttttg ttactgggaa gagctctccg ggtgagaatg gctcctcgca acttcaggaa
361 agtacggctt cggtggtgaa ggaggtcgct caagcagaag aaataagcca agcgttcgag
421 cggtctgaaa gagcatccca ggctgctcag ataaacctaa gcgcaagact ggcaaaggac
481 gggctggcct cggggaagaa gtcaggtctt cgagagatat ggaggttgat cacgattgcg
541 cgtccagagg ctaaggtcct gtctgttgcc tttctgttcc ttctcatctc ctcttcggta
601 gccatgtcta tacccttctc tatcggtaag atccttgaca ttgcgaccag ctccaatccc
661 aatgctttga aagattttct agggatcagt ctccctgtct tctatacagc actgggaggg
721 attattctgc tcggggctgc tgcaaactac ggaaggataa tcatcttgcg aatcgttggt
781 gaacgaattg ttgccagact ccgagctaaa ctcttcagaa acaccttcgt tcaagatgcc
841 gagttctttg atgcaaatcg agttggtgac ctgatctccc ggttgagctc tgataccatc
901 attgtgggga agagtattac acagaatctt tcagacggtc tacgagcagc tgtcagtggc
961 gctgccggat ttggaatgat ggcatatgtc agtttgaagc tctcaagtgt tttggctctt
1021 ttgttccctc caattgctct tgcggctttc ttctacggac gagcaatccg taacctgagc
1081 cggaagatac agaagaatgt tggcacattg accaaaattg cagaggaacg gctcggtaat
1141 gttaaaacaa gccagtcctt tgctggtgag atcttggaag ttcatcgata caatcgtcag
1201 gtcaagcgaa tatttgagct tggtaagaaa gaatcgttca tcagtgcaac cttttttagc
1261 ggcacaagct tgatgggaaa tgctacaata cttgctctcc tttatgttgg tggtgggatg
1321 gttcagtcac aagcaatttc aatcggagaa ctcacctcct ttttaatgta caccgcgtac
1381 gctggttcca gtctttttgg cctttcgagt ttctattcag agctcatgaa aggagcaggt
1441 gctgccagtc gcctgttcga gttacaagac cgcaggccga agatatcccc taccgtgggt
1501 gacaaggtca cctcagctcg cgggcctatc agattcaaaa atgttgcctt cagctatcct
1561 actcgaccag ccgtcactat tttcaaagat ctggattttg agataccgca gggtagcaat
1621 gtggctattg ttggaccctc aggtggtgga aaatccacca ttgcgtcatt gctacttcgt
1681 ttctacagcc caactcaggg ccaagtcttc atcaacggca aggatgttgc aaccatgaac
1741 gcaaagtctc tccgaaggaa gataggaatc gttgcccaag agccagtgtt gttttctggc
1801 tccattgccg aaaatattag ctatggaaaa cctgatgcaa caagatctca gataatcgct
1861 gctgcccgca aagcaaactg ccagttcatc agcgacttcc ctgatggttt ggataccaat
1921 gttggtccca aaggagcaca actgtccggt ggccaaaaac agagaattgc catcgctcgt
1981 gctcttatca aagagccgga tattctcatt cttgatgaag ctacctctgc gctcgatgca
2041 gagtccgaaa ccctcgttaa cagtgctctt gcagctctgc ttcgcgagaa caacactact
2101 atcagcatag cccatcgttt atccaccatc aaacgatccg acaccatcat cgttcttgac
2161 tcggagggca aagttgctga aatgggatct tacaatgatc tcaattcaag accagatggt
2221 gcttttacca agctgatggc ctggcagctc agcggcggcg aaggaattgc tgctcccgtt
2281 caccccaaca agccaatctc tagagggccg ccgtcagaaa cagaacatct gcagcaattg
2341 ctacaagaag gcgaagacga ggagtacgat gaagccgagc ccgaagacga gccgaggtct
2401 actgatgaga ttgaaaagaa ataggcggcc gcttg
//