LOCUS MF048869 6847 bp DNA linear SYN 30-MAY-2017
DEFINITION Synthetic construct (code 12) clone p416-CtSHS, complete sequence.
ACCESSION MF048869
VERSION MF048869.1
KEYWORDS .
SOURCE synthetic construct (code 12)
ORGANISM synthetic construct (code 12)
other sequences; artificial sequences.
REFERENCE 1 (bases 1 to 6847)
AUTHORS Bijlani,S. and Ganesan,K.
TITLE A Cassette for One-Step Gene Deletion and Regulated Gene Expression
in Clinical Isolates of Diploid Candida species
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 6847)
AUTHORS Bijlani,S. and Ganesan,K.
TITLE Direct Submission
JOURNAL Submitted (04-MAY-2017) Yeast Molecular Biology, CSIR- Institute of
Microbial Technology, Sector 39-A, Chandigarh, Chandigarh 160036,
India
COMMENT ##Assembly-Data-START##
Sequencing Technology :: Sanger dideoxy sequencing
##Assembly-Data-END##
FEATURES Location/Qualifiers
source 1..6847
/organism="synthetic construct (code 12)"
/mol_type="other DNA"
/db_xref="taxon:2005482"
/clone="p416-CtSHS"
misc_feature 1..34
/note="FLP recombination target sequence FRT"
regulatory 39..679
/regulatory_class="promoter"
/note="CtMAL2 promoter; derived from Candida tropicalis"
gene 686..1957
/gene="FLP"
CDS 686..1957
/gene="FLP"
/codon_start=1
/transl_table=12
/product="site-specific recombinase"
/protein_id="ART66087.1"
/translation="MPQFDILCKTPPKVLVRQFVERFERPSGEKIALCAAELTYLCWM
ITHNGTAIKRATFMSYNTIISNSLSFDIVNKSLQFKYKTQKATILEASLKKLIPAWEF
TIIPYYGQKHQSDITDIVSSLQLQFESSEEADKGNSHSKKMLKALLSEGESIWEITEK
ILNSFEYTSRFTKTKTLYQFLFLATFINCGRFSDIKNVDPKSFKLVQNKYLGVIIQCL
VTETKTSVSRHIYFFSARGRIDPLVYLDEFLRNSEPVLKRVNRTGNSSSNKQEYQLLK
DNLVRSYNKALKKNAPYSIFAIKNGPKSHIGRHLMTSFLSMKGLTELTNVVGNWSDKR
ASAVARTTYTHQITAIPDHYFALVSRYYAYDPISKEMIALKDETNPIEEWQHIEQLKG
SAEGSIRYPAWNGIISQEVLDYLSSYINRRI"
regulatory 1958..2345
/regulatory_class="terminator"
/note="CaACT1 terminator; derived from Candida albicans"
regulatory 2352..2849
/regulatory_class="promoter"
/note="CaACT1 promoter; derived from Candida albicans"
gene 2850..3924
/gene="SAT1"
/note="5' region; derived from C. albicans codon-optimized
SAT1 gene"
repeat_region 3611..3924
/gene="SAT1"
/rpt_type=direct
gene 3931..6189
/gene="HygB"
/note="codon-optimized for C. albicans"
regulatory 3931..4571
/regulatory_class="promoter"
/gene="HygB"
/note="CaTEF2 promoter; derived from C. albicans"
CDS 4578..5603
/gene="HygB"
/note="hygromycin resistance marker"
/codon_start=1
/transl_table=12
/product="HygB"
/protein_id="ART66088.1"
/translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQD
LPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVY
HWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWS
EAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQ
SLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPS
TRPRAKE"
regulatory 5610..6189
/regulatory_class="terminator"
/gene="HygB"
/note="CaPFK2 terminator; derived from C. albicans"
gene 6190..6655
/gene="SAT1"
/note="3' region; derived from C. albicans codon-optimized
SAT1 gene"
repeat_region 6190..6503
/gene="SAT1"
/rpt_type=direct
misc_feature 6793..6826
/note="FLP recombination target sequence FRT"
BASE COUNT 2107 a 1128 c 1247 g 2365 t
ORIGIN
1 gaagttccta tactttctag agaataggaa cttcggatgg tagtttatag agaatataca
61 aacaattggt gatttatatt tataaaagtt aaaagccctg tataccgaat tactaagaaa
121 ttattgtctg atttgtaatt cagaacgcaa agtgtaatgc cctgtatctt attttttttt
181 ttttgcagct atacgataag aatctcattt ttttggtgcc aatgcgatcg tttatacgca
241 attcctaatt gaaacgtctc aacaaaaaaa ttgtaaatca tcttcaattg gtagctttaa
301 taactctaaa ttcaaggggg ggattgccag cgtatctgta taaacgagtg ttcttttcta
361 ctacattgct gtggatacta ttaatagaca aacagtagtg tgtgtataca catacagaat
421 ccatctctct ccgccacatt gtttccaata aaaaaatatt atccagtaat tggtgttttt
481 gtgtttctat ggatcaattc tcggaaaaat tatcgcgaga acaaagagca agtgtgggga
541 gatggggagt caacacagca agagaacaaa agctttaaat agtccatctt ttcaacggac
601 aagctgaaaa aaaagagcaa ttacttgtta ctcttcaatt ttttttttct ctacttttta
661 tatattcttc aaacaaaacg tcgacatgcc acaatttgat atattatgta aaacaccacc
721 taaggtgctt gttcgtcagt ttgtggaaag gtttgaaaga ccttcaggtg agaaaatagc
781 attatgtgct gctgaactaa cctatttatg ttggatgatt acacataacg gaacagcaat
841 caagagagcc acattcatga gctataatac tatcataagc aattcgttga gtttcgatat
901 tgtcaataaa tcactccagt ttaaatacaa gacgcaaaaa gcaacaattt tggaagcctc
961 attaaagaaa ttgattcctg cttgggaatt tacaattatt ccttactatg gacaaaaaca
1021 tcaatctgat atcactgata ttgtaagtag tttgcaatta cagttcgaat catcggaaga
1081 agcagataag ggaaatagcc acagtaaaaa aatgcttaaa gcacttctaa gtgagggtga
1141 aagcatctgg gagatcactg agaaaatact aaattcgttt gagtatactt cgagatttac
1201 aaaaacaaaa actttatacc aattcctctt cctagctact ttcatcaatt gtggaagatt
1261 cagcgatatt aagaacgttg atccgaaatc atttaaatta gtccaaaata agtatttggg
1321 agtaataatc cagtgtttag tgacagagac aaagacaagc gttagtaggc acatatactt
1381 ctttagcgca aggggtagga tcgatccact tgtatatttg gatgaatttt tgaggaattc
1441 tgaaccagtc ctaaaacgag taaataggac cggcaattct tcaagcaata aacaggaata
1501 ccaattatta aaagataact tagtcagatc gtacaataaa gctttgaaga aaaatgcgcc
1561 ttattcaatc tttgctataa aaaatggccc aaaatctcac attggaagac atttgatgac
1621 ctcatttctt tcaatgaagg gcctaacgga gttgactaat gttgtgggaa attggagcga
1681 taagcgtgct tctgccgtgg ccaggacaac gtatactcat cagataacag caatacctga
1741 tcactacttc gcactagttt ctcggtacta tgcatatgat ccaatatcaa aggaaatgat
1801 agcattgaag gatgagacta atccaattga ggagtggcag catatagaac agctaaaggg
1861 tagtgctgaa ggaagcatac gataccccgc atggaatggg ataatatcac aggaggtact
1921 agactacctt tcatcctaca taaatagacg catataagag tgaaattctg gaaatctgga
1981 aatctggttt tgtattcttg ttattcttct ttttgttatt acatatataa cttgttactt
2041 ttttaaaaaa atctttgtat attttataaa tatataaaac taaatttaag aaaaagagaa
2101 aaatgtttta tttgagagat tgaaatttta cttgaattta gcttagcttt tataaagtat
2161 tattatgtaa aaaaacaaaa caaatataca ttaaaaagtt aagactataa aatagccacc
2221 caaggcattt ctatatcttg ttgttgttgt tttcatcttc tgtatcagag gaacttattt
2281 tattattttc gtcacgggta ttttctcttg tttgatgatt catcccattc attccatcat
2341 aaaatgtcga gcgtcaaaac tagagaataa taaagaaaac gatcttttca aaaagaaaaa
2401 accttttagt tttcctttgt tgttgttgtg ggtgtgtgct atttatatta tatagtttac
2461 tcataatacc ataaaatatt cggtttgatt aggttatttt aataagctaa tttgtttcta
2521 atcgtgtaat ttatgctgtg tatattaagt agtgtgtgca ctgcccaaaa atgtttgttg
2581 tttatagtcg gttaaagaga aaaaagaaaa aaagatccat acacacacgt taattagttg
2641 ttcaacgtaa tacactcata ttttgttctt atttgctttc ggtcgctgtt ctcaccaaga
2701 tttattgcca acgaaacaat ttttttttat atattttcag atttttcttt ttttcctttc
2761 ctttcctttt ctaattttca ctcctggttt tctttctttc ttagaaacat tatctcgata
2821 ttaatattaa aaaaatataa tcattcaaaa tggacggtgg tatgttttag tttagcttca
2881 attctaattg attgattaat cagttgattg gtttcaatat gacaaatggg tagggtggga
2941 aaacttcatt ttcaattcag atcaaacttt tttgttgtcg acataatatt tctcgtttgg
3001 gatgttactg tcacattaat aatacacaca catcagctta taattttgaa agtaatttat
3061 cagatatgtt gtgacgatca atggaaatgg ctaacttcaa tgtatctgtt cttccccttt
3121 ttcaaagttc acgttttttg attgattgat tgatctgtcg gcagtggttt caaaaccatt
3181 cggtgagtaa tcctatcaat caatgttacg acaaaaggct caatattcaa aattgcaatg
3241 ttttatgttt tcctacgtgt acttgtgcaa ggcaattgat tcaacattgc ttttggtgtt
3301 tgacgagttt ctagtttgga cttgtgttgt tatctgggct atacagattt cccggctcac
3361 tatgaatttt ttttttcgac gctcagtgca cacaactata aacaacacaa acacaaacac
3421 agcaagaaaa aaaaaaaacg aacattgaat tgaaaccaag ccaactgaaa aattccttat
3481 ttaaatgact gtcatactaa cccattttta tagaagaagt tgctgcttta gttatcgata
3541 acggttctca tatgaaaatt tcggtgatcc ctgagcaggt ggcggaaaca ttggatgctg
3601 agaaccattt cattgttcgt gaagtgttcg atgtgcacct atccgaccaa ggctttgaac
3661 tatctaccag aagtgtgagc ccctaccgga aggattacat ctcggatgat gactctgatg
3721 aagactctgc ttgctatggc gcattcatcg accaagagct tgtcgggaag attgaactca
3781 actcaacatg gaacgatcta gcctctatcg aacacattgt tgtgtcgcac acgcaccgag
3841 gcaaaggagt cgcgcacagt ctcatcgaat ttgcgaaaaa gtgggcacta agcagacagc
3901 tccttggcat acgattagag acacgacgtc gtatagtgct tgctgttcga tattgctaga
3961 ggcaaagaaa aaggattacc tcacgcctaa aatggaattt gaaaattgag gagatgagtt
4021 gagcttttgt cttttacact ttccctattc aattgtgccg caaaaacttc aatatctaac
4081 acagtgtgaa atatttgtta tgtataccga aagccaaata ccatttaaac tggtatttca
4141 agatttcata aatcaagttt ctggttaaat taattgctgt taaacagaac aagagcttca
4201 cattgatgtt ggaataacta ctatagaatt ataaaataca caaaaccagg tcgtgtacgc
4261 aataactttt caatgtatga aaaaattttt tttttttttt tgcttcttct cttcgcttct
4321 tttttctagt tatcaatttg gcaatgaatt gatttgattg ccctttgttt agtttactat
4381 gaaaaaaaat tttttttttg tctctgtcag attttctctc attttttttt ttgcctcata
4441 atgaacaact caaaaaagaa tataaatttc tcatcaaaga cctttccaat tgaaaatttt
4501 ttctttcctt cttttccttt ttttttttct ttctttcttc ttttctaagt atacatttta
4561 tataatcaat cctcgagatg aaaaaaccag aattgactgc tacttctgtt gaaaaatttt
4621 tgattgaaaa atttgattct gtttctgatt tgatgcaatt gtctgaaggt gaagaatcaa
4681 gagctttttc ttttgatgtt ggtggtagag gttatgtttt gagagttaat tcttgtgctg
4741 atggttttta taaagataga tatgtttata gacattttgc ttctgctgct ttgccaattc
4801 cagaagtttt ggatattggt gaattttctg aatctttgac ttattgtatt tcaagaagag
4861 ctcaaggtgt tactttgcaa gatttgccag aaactgaatt gccagctgtt ttgcaaccag
4921 ttgctgaagc tatggatgct attgctgctg ctgatttgtc tcaaacttct ggttttggtc
4981 catttggtcc acaaggtatt ggtcaatata ctacttggag agattttatt tgtgctattg
5041 ctgatccaca tgtttatcat tggcaaactg ttatggatga tactgtttct gcttctgttg
5101 ctcaagcatt ggatgaattg atgttgtggg ctgaagattg tccagaagtt agacatttgg
5161 ttcatgctga ttttggttct aataatgttt tgactgataa tggtagaatt actgctgtta
5221 ttgattggtc tgaagctatg tttggtgatt ctcaatatga agttgctaat attttttttt
5281 ggagaccatg gttggcttgt atggaacaac aaactagata ttttgaaaga agacatccag
5341 aattggctgg ttctccaaga ttgagagctt atatgttgag aattggtttg gatcaattgt
5401 atcaatcttt ggttgatggt aattttgatg atgctgcttg ggctcaaggt agatgtgatg
5461 ctattgttag atcaggtgct ggtactgttg gtagaactca aattgctaga agatcagctg
5521 ctgtttggac tgatggttgt gttgaagttt tggctgattc tggtaataga agaccatcta
5581 ctagaccaag agctaaagaa taaggatccg acgaagttgg tgtatgataa cgaatttata
5641 gtttttatat tgtaaaataa tgaaatattt gttttttttt tactttgatg tatatgctgt
5701 atcccaatcc tcgatacatt gcagaattga atcatttggt ttatctgttg ttttgtgcta
5761 agtaagatat tttccatcta gaagtgatag ctttttatta tcaaattaca atccctatta
5821 catgtggaca tcaactccat ttctttgcgc attttatagt agagttagag ttagcttgtc
5881 acatctcgtt gcttcctttt gctgcaactg tttccgattt caaaatgaaa atagcttaaa
5941 gttacccgga gggcctgcac ataatcaaaa tatagtaaat tcccaaaaga acagtcgtgt
6001 gcagtttcca aagtgaaatt ttttttttgt ttctctcact tccttgtaag gttgtttccc
6061 tcttctttct ctctaaccaa tcctttgtag attgaaaatt ttttcttgca aatttttctt
6121 taactctctt tccctttttt tttcgaatca aggtaccata tcattaccaa attagacatc
6181 atgcctatgc attgttcgtg aagtgttcga tgtgcaccta tccgaccaag gctttgaact
6241 atctaccaga agtgtgagcc cctaccggaa ggattacatc tcggatgatg actctgatga
6301 agactctgct tgctatggcg cattcatcga ccaagagctt gtcgggaaga ttgaactcaa
6361 ctcaacatgg aacgatctag cctctatcga acacattgtt gtgtcgcaca cgcaccgagg
6421 caaaggagtc gcgcacagtc tcatcgaatt tgcgaaaaag tgggcactaa gcagacagct
6481 ccttggcata cgattagaga cacaaacgaa caatgtacct gcctgcaatt tgtacgcaaa
6541 atgtggcttt actctcggcg gcattgacct cttcacgtat aaaactagac ctcaagtctc
6601 gaacgaaaca gcgatgtact ggtactggtt ctcgggagca caggatgacg cctaacatat
6661 gtgaagtgtg aagggggaga ttttcacttt attagatttg tatatatgta taataaataa
6721 ataaataagt taaataaata attagataag ggtggtaatt attactattt acaatcaaag
6781 gtggtcctgc aggaagttcc tatactttct agagaatagg aacttcagat ccactagttc
6841 tagagcg
//