LOCUS MF048868 6754 bp DNA linear SYN 30-MAY-2017
DEFINITION Synthetic construct (code 12) clone p416-SHS, complete sequence.
ACCESSION MF048868
VERSION MF048868.1
KEYWORDS .
SOURCE synthetic construct (code 12)
ORGANISM synthetic construct (code 12)
other sequences; artificial sequences.
REFERENCE 1 (bases 1 to 6754)
AUTHORS Bijlani,S. and Ganesan,K.
TITLE A Cassette for One-Step Gene Deletion and Regulated Gene Expression
in Clinical Isolates of Diploid Candida species
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 6754)
AUTHORS Bijlani,S. and Ganesan,K.
TITLE Direct Submission
JOURNAL Submitted (04-MAY-2017) Yeast Molecular Biology, CSIR- Institute of
Microbial Technology, Sector 39-A, Chandigarh, Chandigarh 160036,
India
COMMENT ##Assembly-Data-START##
Sequencing Technology :: Sanger dideoxy sequencing
##Assembly-Data-END##
FEATURES Location/Qualifiers
source 1..6754
/organism="synthetic construct (code 12)"
/mol_type="other DNA"
/db_xref="taxon:2005482"
/clone="p416-SHS"
misc_feature 1..34
/note="FLP recombination target sequence FRT"
regulatory 39..586
/regulatory_class="promoter"
/note="CaMAL2 promoter; derived from Candida albicans"
gene 593..1864
/gene="FLP"
CDS 593..1864
/gene="FLP"
/codon_start=1
/transl_table=12
/product="site-specific recombinase"
/protein_id="ART66085.1"
/translation="MPQFDILCKTPPKVLVRQFVERFERPSGEKIALCAAELTYLCWM
ITHNGTAIKRATFMSYNTIISNSLSFDIVNKSLQFKYKTQKATILEASLKKLIPAWEF
TIIPYYGQKHQSDITDIVSSLQLQFESSEEADKGNSHSKKMLKALLSEGESIWEITEK
ILNSFEYTSRFTKTKTLYQFLFLATFINCGRFSDIKNVDPKSFKLVQNKYLGVIIQCL
VTETKTSVSRHIYFFSARGRIDPLVYLDEFLRNSEPVLKRVNRTGNSSSNKQEYQLLK
DNLVRSYNKALKKNAPYSIFAIKNGPKSHIGRHLMTSFLSMKGLTELTNVVGNWSDKR
ASAVARTTYTHQITAIPDHYFALVSRYYAYDPISKEMIALKDETNPIEEWQHIEQLKG
SAEGSIRYPAWNGIISQEVLDYLSSYINRRI"
regulatory 1865..2252
/regulatory_class="terminator"
/note="CaACT1 terminator; derived from Candida albicans"
regulatory 2259..2756
/regulatory_class="promoter"
/note="CaACT1 promoter; derived from Candida albicans"
gene 2757..3831
/gene="SAT1"
/note="5' region; derived from C, albicans codon-optimized
SAT1 gene"
repeat_region 3518..3831
/gene="SAT1"
/rpt_type=direct
gene 3838..6096
/gene="HygB"
/note="codon-optimized for C. albicans"
regulatory 3838..4478
/regulatory_class="promoter"
/gene="HygB"
/note="CaTEF2 promoter; derived from C. albicans"
CDS 4485..5510
/gene="HygB"
/codon_start=1
/transl_table=12
/product="hygromycin resistance marker"
/protein_id="ART66086.1"
/translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQD
LPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVY
HWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWS
EAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQ
SLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPS
TRPRAKE"
regulatory 5517..6096
/regulatory_class="terminator"
/gene="HygB"
/note="CaPFK2 terminator; derived from C. albicans"
gene 6097..6562
/gene="SAT1"
/note="3' region; derived from C. albicans codon-optimized
SAT1 gene"
repeat_region 6097..6410
/gene="SAT1"
/rpt_type=direct
misc_feature 6700..6733
/note="FLP recombination target sequence FRT"
BASE COUNT 2049 a 1120 c 1247 g 2338 t
ORIGIN
1 gaagttccta tactttctag agaataggaa cttcggatcc aataatgatt ggtttgatat
61 ttttgtctag taccatctgt accattacac ttaaattatc tttatatctg tctaactcga
121 ctgtctggat ttcattgatg tagtcgtatg catcgttagt tccaaaaaat attgtcatca
181 atttgatatt ggtttccgac tctaaaattt ttggaagaat ttgtctagcg tgctctgagt
241 tgtagccact gaaaccacgg ttaataacat ccaattttcg gatatacaca ttctgtaatg
301 ctggatgaaa gccatactgg gtacaactaa actgggtgat ggagtcaccg aacaacacaa
361 atttaccgta ttccatgatt gctatggttg agaatttttt ttttttcttg tcccacgcca
421 tttttcaaat tatgcagttg agaatgttag tttttgtgta caccccgttc gctgaatatt
481 tcggaataat tcaaagattg gggagtgggg gaggcgatag acgaagacac ggtataaaaa
541 tgggcaaaat tttccccaac tttttgcagt ggtttaacta ataatcgtcg acatgccaca
601 atttgatata ttatgtaaaa caccacctaa ggtgcttgtt cgtcagtttg tggaaaggtt
661 tgaaagacct tcaggtgaga aaatagcatt atgtgctgct gaactaacct atttatgttg
721 gatgattaca cataacggaa cagcaatcaa gagagccaca ttcatgagct ataatactat
781 cataagcaat tcgttgagtt tcgatattgt caataaatca ctccagttta aatacaagac
841 gcaaaaagca acaattttgg aagcctcatt aaagaaattg attcctgctt gggaatttac
901 aattattcct tactatggac aaaaacatca atctgatatc actgatattg taagtagttt
961 gcaattacag ttcgaatcat cggaagaagc agataaggga aatagccaca gtaaaaaaat
1021 gcttaaagca cttctaagtg agggtgaaag catctgggag atcactgaga aaatactaaa
1081 ttcgtttgag tatacttcga gatttacaaa aacaaaaact ttataccaat tcctcttcct
1141 agctactttc atcaattgtg gaagattcag cgatattaag aacgttgatc cgaaatcatt
1201 taaattagtc caaaataagt atttgggagt aataatccag tgtttagtga cagagacaaa
1261 gacaagcgtt agtaggcaca tatacttctt tagcgcaagg ggtaggatcg atccacttgt
1321 atatttggat gaatttttga ggaattctga accagtccta aaacgagtaa ataggaccgg
1381 caattcttca agcaataaac aggaatacca attattaaaa gataacttag tcagatcgta
1441 caataaagct ttgaagaaaa atgcgcctta ttcaatcttt gctataaaaa atggcccaaa
1501 atctcacatt ggaagacatt tgatgacctc atttctttca atgaagggcc taacggagtt
1561 gactaatgtt gtgggaaatt ggagcgataa gcgtgcttct gccgtggcca ggacaacgta
1621 tactcatcag ataacagcaa tacctgatca ctacttcgca ctagtttctc ggtactatgc
1681 atatgatcca atatcaaagg aaatgatagc attgaaggat gagactaatc caattgagga
1741 gtggcagcat atagaacagc taaagggtag tgctgaagga agcatacgat accccgcatg
1801 gaatgggata atatcacagg aggtactaga ctacctttca tcctacataa atagacgcat
1861 ataagagtga aattctggaa atctggaaat ctggttttgt attcttgtta ttcttctttt
1921 tgttattaca tatataactt gttacttttt taaaaaaatc tttgtatatt ttataaatat
1981 ataaaactaa atttaagaaa aagagaaaaa tgttttattt gagagattga aattttactt
2041 gaatttagct tagcttttat aaagtattat tatgtaaaaa aacaaaacaa atatacatta
2101 aaaagttaag actataaaat agccacccaa ggcatttcta tatcttgttg ttgttgtttt
2161 catcttctgt atcagaggaa cttattttat tattttcgtc acgggtattt tctcttgttt
2221 gatgattcat cccattcatt ccatcataaa atgtcgagcg tcaaaactag agaataataa
2281 agaaaacgat cttttcaaaa agaaaaaacc ttttagtttt cctttgttgt tgttgtgggt
2341 gtgtgctatt tatattatat agtttactca taataccata aaatattcgg tttgattagg
2401 ttattttaat aagctaattt gtttctaatc gtgtaattta tgctgtgtat attaagtagt
2461 gtgtgcactg cccaaaaatg tttgttgttt atagtcggtt aaagagaaaa aagaaaaaaa
2521 gatccataca cacacgttaa ttagttgttc aacgtaatac actcatattt tgttcttatt
2581 tgctttcggt cgctgttctc accaagattt attgccaacg aaacaatttt tttttatata
2641 ttttcagatt tttctttttt tcctttcctt tccttttcta attttcactc ctggttttct
2701 ttctttctta gaaacattat ctcgatatta atattaaaaa aatataatca ttcaaaatgg
2761 acggtggtat gttttagttt agcttcaatt ctaattgatt gattaatcag ttgattggtt
2821 tcaatatgac aaatgggtag ggtgggaaaa cttcattttc aattcagatc aaactttttt
2881 gttgtcgaca taatatttct cgtttgggat gttactgtca cattaataat acacacacat
2941 cagcttataa ttttgaaagt aatttatcag atatgttgtg acgatcaatg gaaatggcta
3001 acttcaatgt atctgttctt cccctttttc aaagttcacg ttttttgatt gattgattga
3061 tctgtcggca gtggtttcaa aaccattcgg tgagtaatcc tatcaatcaa tgttacgaca
3121 aaaggctcaa tattcaaaat tgcaatgttt tatgttttcc tacgtgtact tgtgcaaggc
3181 aattgattca acattgcttt tggtgtttga cgagtttcta gtttggactt gtgttgttat
3241 ctgggctata cagatttccc ggctcactat gaattttttt tttcgacgct cagtgcacac
3301 aactataaac aacacaaaca caaacacagc aagaaaaaaa aaaaacgaac attgaattga
3361 aaccaagcca actgaaaaat tccttattta aatgactgtc atactaaccc atttttatag
3421 aagaagttgc tgctttagtt atcgataacg gttctcatat gaaaatttcg gtgatccctg
3481 agcaggtggc ggaaacattg gatgctgaga accatttcat tgttcgtgaa gtgttcgatg
3541 tgcacctatc cgaccaaggc tttgaactat ctaccagaag tgtgagcccc taccggaagg
3601 attacatctc ggatgatgac tctgatgaag actctgcttg ctatggcgca ttcatcgacc
3661 aagagcttgt cgggaagatt gaactcaact caacatggaa cgatctagcc tctatcgaac
3721 acattgttgt gtcgcacacg caccgaggca aaggagtcgc gcacagtctc atcgaatttg
3781 cgaaaaagtg ggcactaagc agacagctcc ttggcatacg attagagaca cgacgtcgta
3841 tagtgcttgc tgttcgatat tgctagaggc aaagaaaaag gattacctca cgcctaaaat
3901 ggaatttgaa aattgaggag atgagttgag cttttgtctt ttacactttc cctattcaat
3961 tgtgccgcaa aaacttcaat atctaacaca gtgtgaaata tttgttatgt ataccgaaag
4021 ccaaatacca tttaaactgg tatttcaaga tttcataaat caagtttctg gttaaattaa
4081 ttgctgttaa acagaacaag agcttcacat tgatgttgga ataactacta tagaattata
4141 aaatacacaa aaccaggtcg tgtacgcaat aacttttcaa tgtatgaaaa aatttttttt
4201 ttttttttgc ttcttctctt cgcttctttt ttctagttat caatttggca atgaattgat
4261 ttgattgccc tttgtttagt ttactatgaa aaaaaatttt ttttttgtct ctgtcagatt
4321 ttctctcatt tttttttttg cctcataatg aacaactcaa aaaagaatat aaatttctca
4381 tcaaagacct ttccaattga aaattttttc tttccttctt ttcctttttt tttttctttc
4441 tttcttcttt tctaagtata cattttatat aatcaatcct cgagatgaaa aaaccagaat
4501 tgactgctac ttctgttgaa aaatttttga ttgaaaaatt tgattctgtt tctgatttga
4561 tgcaattgtc tgaaggtgaa gaatcaagag ctttttcttt tgatgttggt ggtagaggtt
4621 atgttttgag agttaattct tgtgctgatg gtttttataa agatagatat gtttatagac
4681 attttgcttc tgctgctttg ccaattccag aagttttgga tattggtgaa ttttctgaat
4741 ctttgactta ttgtatttca agaagagctc aaggtgttac tttgcaagat ttgccagaaa
4801 ctgaattgcc agctgttttg caaccagttg ctgaagctat ggatgctatt gctgctgctg
4861 atttgtctca aacttctggt tttggtccat ttggtccaca aggtattggt caatatacta
4921 cttggagaga ttttatttgt gctattgctg atccacatgt ttatcattgg caaactgtta
4981 tggatgatac tgtttctgct tctgttgctc aagcattgga tgaattgatg ttgtgggctg
5041 aagattgtcc agaagttaga catttggttc atgctgattt tggttctaat aatgttttga
5101 ctgataatgg tagaattact gctgttattg attggtctga agctatgttt ggtgattctc
5161 aatatgaagt tgctaatatt tttttttgga gaccatggtt ggcttgtatg gaacaacaaa
5221 ctagatattt tgaaagaaga catccagaat tggctggttc tccaagattg agagcttata
5281 tgttgagaat tggtttggat caattgtatc aatctttggt tgatggtaat tttgatgatg
5341 ctgcttgggc tcaaggtaga tgtgatgcta ttgttagatc aggtgctggt actgttggta
5401 gaactcaaat tgctagaaga tcagctgctg tttggactga tggttgtgtt gaagttttgg
5461 ctgattctgg taatagaaga ccatctacta gaccaagagc taaagaataa ggatccgacg
5521 aagttggtgt atgataacga atttatagtt tttatattgt aaaataatga aatatttgtt
5581 ttttttttac tttgatgtat atgctgtatc ccaatcctcg atacattgca gaattgaatc
5641 atttggttta tctgttgttt tgtgctaagt aagatatttt ccatctagaa gtgatagctt
5701 tttattatca aattacaatc cctattacat gtggacatca actccatttc tttgcgcatt
5761 ttatagtaga gttagagtta gcttgtcaca tctcgttgct tccttttgct gcaactgttt
5821 ccgatttcaa aatgaaaata gcttaaagtt acccggaggg cctgcacata atcaaaatat
5881 agtaaattcc caaaagaaca gtcgtgtgca gtttccaaag tgaaattttt tttttgtttc
5941 tctcacttcc ttgtaaggtt gtttccctct tctttctctc taaccaatcc tttgtagatt
6001 gaaaattttt tcttgcaaat ttttctttaa ctctctttcc cttttttttt cgaatcaagg
6061 taccatatca ttaccaaatt agacatcatg cctatgcatt gttcgtgaag tgttcgatgt
6121 gcacctatcc gaccaaggct ttgaactatc taccagaagt gtgagcccct accggaagga
6181 ttacatctcg gatgatgact ctgatgaaga ctctgcttgc tatggcgcat tcatcgacca
6241 agagcttgtc gggaagattg aactcaactc aacatggaac gatctagcct ctatcgaaca
6301 cattgttgtg tcgcacacgc accgaggcaa aggagtcgcg cacagtctca tcgaatttgc
6361 gaaaaagtgg gcactaagca gacagctcct tggcatacga ttagagacac aaacgaacaa
6421 tgtacctgcc tgcaatttgt acgcaaaatg tggctttact ctcggcggca ttgacctctt
6481 cacgtataaa actagacctc aagtctcgaa cgaaacagcg atgtactggt actggttctc
6541 gggagcacag gatgacgcct aacatatgtg aagtgtgaag ggggagattt tcactttatt
6601 agatttgtat atatgtataa taaataaata aataagttaa ataaataatt agataagggt
6661 ggtaattatt actatttaca atcaaaggtg gtcctgcagg aagttcctat actttctaga
6721 gaataggaac ttcagatcca ctagttctag agcg
//