LOCUS MF048863 2334 bp DNA linear SYN 30-MAY-2017
DEFINITION Synthetic construct (code 12) clone pYM71P, complete sequence.
ACCESSION MF048863
VERSION MF048863.1
KEYWORDS .
SOURCE synthetic construct (code 12)
ORGANISM synthetic construct (code 12)
other sequences; artificial sequences.
REFERENCE 1 (bases 1 to 2334)
AUTHORS Bijlani,S. and Ganesan,K.
TITLE A Cassette for One-Step Gene Deletion and Regulated Gene Expression
in Clinical Isolates of Diploid Candida species
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 2334)
AUTHORS Bijlani,S. and Ganesan,K.
TITLE Direct Submission
JOURNAL Submitted (04-MAY-2017) Yeast Molecular Biology, CSIR- Institute of
Microbial Technology, Sector 39-A, Chandigarh, Chandigarh 160036,
India
COMMENT ##Assembly-Data-START##
Sequencing Technology :: Sanger dideoxy sequencing
##Assembly-Data-END##
FEATURES Location/Qualifiers
source 1..2334
/organism="synthetic construct (code 12)"
/mol_type="other DNA"
/db_xref="taxon:2005482"
/clone="pYM71P"
gene 3..2264
/gene="HygB"
/note="codon-optimized for Candida albicans"
regulatory 3..645
/regulatory_class="promoter"
/gene="HygB"
/note="CaTEF2 promoter; derived from Candida albicans"
CDS 652..1677
/gene="HygB"
/codon_start=1
/transl_table=12
/product="hygromycin resistance marker"
/protein_id="ART66076.1"
/translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQD
LPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVY
HWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWS
EAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQ
SLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPS
TRPRAKE"
regulatory 1684..2264
/regulatory_class="terminator"
/gene="HygB"
/note="CaPFK2 terminator; derived from Candida albicans"
BASE COUNT 642 a 356 c 419 g 917 t
ORIGIN
1 tcgtatagtg cttgctgttc gatattgcta gaggcaaaga aaaaggatta cctcacgcct
61 aaaatggaat ttgaaaattg aggagatgag ttgagctttt gtcttttaca ctttccctat
121 tcaattgtgc cgcaaaaact tcaatatcta acacagtgtg aaatatttgt tatgtatacc
181 gaaagccaaa taccatttaa actggtattt caagatttca taaatcaagt ttctggttaa
241 attaattgct gttaaacaga acaagagctt cacattgatg ttggaataac tactatagaa
301 ttataaaata cacaaaacca ggtcgtgtac gcaataactt ttcaatgtat gaaaaaattt
361 tttttttttt tttttgcttc ttctcttcgc ttcttttttc tagttatcaa tttggcaatg
421 aattgatttg attgcccttt gtttagttta ctatgaaaaa aaattttttt tttgtctctg
481 tcagattttc tctcattttt ttttttgcct cataatgaac aactcaaaaa agaatataaa
541 tttctcatca aagacctttc caattgaaaa ttttttcttt ccttcttttc cttttttttt
601 ttctttcttt cttcttttct aagtatacat tttatataat caatcctcga gatgaaaaaa
661 ccagaattga ctgctacttc tgttgaaaaa tttttgattg aaaaatttga ttctgtttct
721 gatttgatgc aattgtctga aggtgaagaa tcaagagctt tttcttttga tgttggtggt
781 agaggttatg ttttgagagt taattcttgt gctgatggtt tttataaaga tagatatgtt
841 tatagacatt ttgcttctgc tgctttgcca attccagaag ttttggatat tggtgaattt
901 tctgaatctt tgacttattg tatttcaaga agagctcaag gtgttacttt gcaagatttg
961 ccagaaactg aattgccagc tgttttgcaa ccagttgctg aagctatgga tgctattgct
1021 gctgctgatt tgtctcaaac ttctggtttt ggtccatttg gtccacaagg tattggtcaa
1081 tatactactt ggagagattt tatttgtgct attgctgatc cacatgttta tcattggcaa
1141 actgttatgg atgatactgt ttctgcttct gttgctcaag cattggatga attgatgttg
1201 tgggctgaag attgtccaga agttagacat ttggttcatg ctgattttgg ttctaataat
1261 gttttgactg ataatggtag aattactgct gttattgatt ggtctgaagc tatgtttggt
1321 gattctcaat atgaagttgc taatattttt ttttggagac catggttggc atgtatggaa
1381 caacaaacta gatattttga aagaagacat ccagaattgg ctggttctcc aagattgaga
1441 gcttatatgt tgagaattgg tttggatcaa ttgtatcaat ctttggttga tggtaatttt
1501 gatgatgctg cttgggctca aggtagatgt gatgctattg ttagatcagg tgctggtact
1561 gttggtagaa ctcaaattgc tagaagatca gctgctgttt ggactgatgg ttgtgttgaa
1621 gttttggctg attctggtaa tagaagacca tctactagac caagagctaa agaataagga
1681 tccgacgaag ttggtgtatg ataacgaatt tatagttttt atattgtaaa ataatgaaat
1741 atttgttttt tttttacttt gatgtatatg ctgtatccca atcctcgata cattgcagaa
1801 ttgaatcatt tggtttatct gttgttttgt gctaagtaag atattttcca tctagaagtg
1861 atagcttttt attatcaaat tacaatccct attacatgtg gacatcaact ccatttcttt
1921 gcgcatttta tagtagagtt agagttagct tgtcacatct cgttgcttcc ttttgctgca
1981 actgtttccg atttcaaaat gaaaatagct taaagttacc cggagggcct gcacataatc
2041 aaaatatagt aaattcccaa aagaacagtc gtgtgcagtt tccaaagtga aatttttttt
2101 ttgtttctct cacttccttg taaggttgtt tccctcttct ttctctctaa ccaatccttt
2161 gtagattgaa aattttttct tgcaaatttt tctttaactc tctttccctt tttttttcga
2221 atcaaggtac catatcatta ccaaattaga catcatgcct atgcgacgtc taagaaacca
2281 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtc
//