LOCUS MF048862 4387 bp DNA linear SYN 30-MAY-2017
DEFINITION Synthetic construct (code 12) clone pCAD/CaHygB, complete sequence.
ACCESSION MF048862
VERSION MF048862.1
KEYWORDS .
SOURCE synthetic construct (code 12)
ORGANISM synthetic construct (code 12)
other sequences; artificial sequences.
REFERENCE 1 (bases 1 to 4387)
AUTHORS Bijlani,S. and Ganesan,K.
TITLE Single-Transformation Gene Function Test by Conditional Expression
of Both Alleles of Candida albicans Genes
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 4387)
AUTHORS Bijlani,S. and Ganesan,K.
TITLE Direct Submission
JOURNAL Submitted (04-MAY-2017) Yeast Molecular Biology, CSIR- Institute of
Microbial Technology, Sector 39-A, Chandigarh, Chandigarh 160036,
India
COMMENT ##Assembly-Data-START##
Sequencing Technology :: Sanger dideoxy sequencing
##Assembly-Data-END##
FEATURES Location/Qualifiers
source 1..4387
/organism="synthetic construct (code 12)"
/mol_type="other DNA"
/db_xref="taxon:2005482"
/clone="pCAD/CaHygB"
misc_feature 1..197
/note="CaARG4 sequence for integration within HAH2
cassette"
regulatory 206..731
/regulatory_class="promoter"
/note="CaMAL2 promoter"
CDS 747..1778
/codon_start=1
/transl_table=12
/product="synthetic Cre recombinase"
/protein_id="ART66074.1"
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKM
LLSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRS
GLPRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRN
LAFLGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVT
KLVERWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAK
DDSGQRYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGA
MVRLLEDGD"
regulatory 1807..2449
/regulatory_class="promoter"
/note="CaTEF2 promoter"
CDS 2456..3481
/codon_start=1
/transl_table=12
/product="hygromycin resistance marker"
/protein_id="ART66075.1"
/translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQD
LPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVY
HWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWS
EAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQ
SLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPS
TRPRAKE"
regulatory 3488..4068
/regulatory_class="terminator"
/note="CaPFK2 terminator"
misc_feature 4122..4387
/note="CaARG4 sequence for integration within HAH2
cassette"
BASE COUNT 1260 a 684 c 847 g 1596 t
ORIGIN
1 atctggattt ttaatgtcga tcaaatctat tccatctact tataataaag atatgcaaga
61 ggataaagaa ccattatttg atgcattgac tacagtggaa cattcaattt taattgctac
121 tggtgtgatt tctactttac tgattgataa acaaaatatg gaaaaagctt taactatgga
181 tatgttggct actgattgtt taaacttttg tctagtacca tctgtaccat tacacttaaa
241 ttatctttat atctgtctaa ctcgactgtc tgggtttcat tgatgtagtc gtatgcatcg
301 ttagttccaa aaaatattgt catcaatttg atattggttt ccgactctaa aatttttgga
361 agaatttgtc tagcgtgctc tgagttgtgg ccactgaaac cacggttaat aacatccaat
421 tttcggatat acacattctg taatgctgga tgaaagccat actgggtaca actaaactgg
481 gtgatggagt caccgaacaa cacaaattta ccgtattcca tgattgctat ggttgagaat
541 tttttttttt tcttgtccca cgccattttt caaattatgc agttgagaat gttagttttt
601 gtgtacaccc cgttcgctga atatttcgga ataattcaaa gattggggag tgggggaggc
661 gatagacgaa gacacggtat aaaaatgggc aaaattttcc ccaacttttt gcagtggttt
721 aactaataat caactacaat gactagatgt ctaacttgtt gaccgttcac caaaacttgc
781 cagctttgcc agttgatgct acctctgatg aagttagaaa aaacttgatg gatatgttta
841 gagatagaca agccttctct gaacacacct ggaaaatgtt gttgtctgtt tgtagatcct
901 gggctgcttg gtgtaaattg aacaacagaa aatggttccc agccgaacca gaagatgtta
961 gagattactt gttgtacttg caagctagag gtttggctgt taaaaccatt caacaacact
1021 tgggtcaatt gaacatgttg cacagaagat ccggtttgcc aagaccatct gattctaacg
1081 ctgtttcttt ggttatgaga agaattagaa aagaaaacgt tgatgctggt gaaagagcta
1141 aacaagcctt ggctttcgaa agaaccgatt tcgatcaagt tagatccttg atggaaaact
1201 ctgatagatg tcaagatatt agaaacttgg ctttcttggg tattgcttac aacaccttgt
1261 tgagaattgc tgaaattgct agaattagag ttaaagatat ttccagaacc gatggtggta
1321 gaatgttgat tcacattggt agaaccaaaa ccttggtttc taccgctggt gttgaaaaag
1381 ccttgtcttt gggtgttacc aaattggttg aaagatggat ttctgtttct ggtgttgctg
1441 atgatccaaa caactacttg ttctgtagag ttagaaaaaa cggtgttgct gctccatctg
1501 ctacctctca attatctacc agagctttgg aaggtatttt cgaagctacc cacagattga
1561 tttacggtgc taaagatgat tctggtcaaa gatacttggc ttggtctggt cactctgcta
1621 gagttggtgc tgctagagat atggctagag ccggcgtttc tattccagaa attatgcaag
1681 ctggtggttg gaccaacgtt aacattgtta tgaactacat tagaaacttg gattctgaaa
1741 ccggtgctat ggttagattg ttggaagatg gtgattaact cgagctgcag gcatgcaagc
1801 ttcctcgtat agtgcttgct gttcgatatt gctagaggca aagaaaaagg attacctcac
1861 gcctaaaatg gaatttgaaa attgaggaga tgagttgagc ttttgtcttt tacactttcc
1921 ctattcaatt gtgccgcaaa aacttcaata tctaacacag tgtgaaatat ttgttatgta
1981 taccgaaagc caaataccat ttaaactggt atttcaagat ttcataaatc aagtttctgg
2041 ttaaattaat tgctgttaaa cagaacaaga gcttcacatt gatgttggaa taactactat
2101 agaattataa aatacacaaa accaggtcgt gtacgcaata acttttcaat gtatgaaaaa
2161 attttttttt tttttttttg cttcttctct tcgcttcttt tttctagtta tcaatttggc
2221 aatgaattga tttgattgcc ctttgtttag tttactatga aaaaaaattt tttttttgtc
2281 tctgtcagat tttctctcat tttttttttt gcctcataat gaacaactca aaaaagaata
2341 taaatttctc atcaaagacc tttccaattg aaaatttttt ctttccttct tttccttttt
2401 ttttttcttt ctttcttctt ttctaagtat acattttata taatcaatcc tcgagatgaa
2461 aaaaccagaa ttgactgcta cttctgttga aaaatttttg attgaaaaat ttgattctgt
2521 ttctgatttg atgcaattgt ctgaaggtga agaatcaaga gctttttctt ttgatgttgg
2581 tggtagaggt tatgttttga gagttaattc ttgtgctgat ggtttttata aagatagata
2641 tgtttataga cattttgctt ctgctgcttt gccaattcca gaagttttgg atattggtga
2701 attttctgaa tctttgactt attgtatttc aagaagagct caaggtgtta ctttgcaaga
2761 tttgccagaa actgaattgc cagctgtttt gcaaccagtt gctgaagcta tggatgctat
2821 tgctgctgct gatttgtctc aaacttctgg ttttggtcca tttggtccac aaggtattgg
2881 tcaatatact acttggagag attttatttg tgctattgct gatccacatg tttatcattg
2941 gcaaactgtt atggatgata ctgtttctgc ttctgttgct caagcattgg atgaattgat
3001 gttgtgggct gaagattgtc cagaagttag acatttggtt catgctgatt ttggttctaa
3061 taatgttttg actgataatg gtagaattac tgctgttatt gattggtctg aagctatgtt
3121 tggtgattct caatatgaag ttgctaatat ttttttttgg agaccatggt tggcatgtat
3181 ggaacaacaa actagatatt ttgaaagaag acatccagaa ttggctggtt ctccaagatt
3241 gagagcttat atgttgagaa ttggtttgga tcaattgtat caatctttgg ttgatggtaa
3301 ttttgatgat gctgcttggg ctcaaggtag atgtgatgct attgttagat caggtgctgg
3361 tactgttggt agaactcaaa ttgctagaag atcagctgct gtttggactg atggttgtgt
3421 tgaagttttg gctgattctg gtaatagaag accatctact agaccaagag ctaaagaata
3481 aggatccgac gaagttggtg tatgataacg aatttatagt ttttatattg taaaataatg
3541 aaatatttgt ttttttttta ctttgatgta tatgctgtat cccaatcctc gatacattgc
3601 agaattgaat catttggttt atctgttgtt ttgtgctaag taagatattt tccatctaga
3661 agtgatagct ttttattatc aaattacaat ccctattaca tgtggacatc aactccattt
3721 ctttgcgcat tttatagtag agttagagtt agcttgtcac atctcgttgc ttccttttgc
3781 tgcaactgtt tccgatttca aaatgaaaat agcttaaagt tacccggagg gcctgcacat
3841 aatcaaaata tagtaaattc ccaaaagaac agtcgtgtgc agtttccaaa gtgaaatttt
3901 ttttttgttt ctctcacttc cttgtaaggt tgtttccctc ttctttctct ctaaccaatc
3961 ctttgtagat tgaaaatttt ttcttgcaaa tttttcttta actctctttc cctttttttt
4021 tcgaatcaag gtaccatatc attaccaaat tagacatcat gcctatgcta agcttggcgt
4081 aatcatggtc atagctgttt cctgtgtgaa attgttatcc ggctgaagaa gaaaaattat
4141 ctggaattga tcaattatct tttgaacaat ttcaacaaat tgattctcgt tttgaaaaag
4201 acgttatgga aacttttgat tttgaagcta gtgtggaaag aagagatgct attggtggta
4261 ctgctaaaag tgccgtttta aaacaattag aaaatttaaa atcaatttta agttaattta
4321 taaatagtca tataataatc acagtattgt ttaacttaca taaaatataa tcaattaaca
4381 cagagat
//