LOCUS SYNPUC19V 2686 bp DNA circular SYN 07-AUG-2008
DEFINITION Cloning vector pUC19, complete sequence.
ACCESSION M77789 M11662
VERSION M77789.2
KEYWORDS .
SOURCE Cloning vector pUC19
ORGANISM Cloning vector pUC19
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 2686)
AUTHORS Norrander,J., Kempe,T. and Messing,J.
TITLE Construction of improved M13 vectors using
oligodeoxynucleotide-directed mutagenesis
JOURNAL Gene 26 (1), 101-106 (1983)
PUBMED 6323249
REFERENCE 2 (bases 1 to 2686)
AUTHORS Yanisch-Perron,C., Vieira,J. and Messing,J.
TITLE Improved M13 phage cloning vectors and host strains: nucleotide
sequences of the M13mp18 and pUC19 vectors
JOURNAL Gene 33 (1), 103-119 (1985)
PUBMED 2985470
REMARK Erratum:[Gene. 1992 May 1;114(1):81-3. PMID: 1375182]
REFERENCE 3 (bases 1 to 2686)
AUTHORS Messing,J.
TITLE Direct Submission
JOURNAL Submitted (27-SEP-1991) Waksman Institute, Rutgers State Univ., 190
Frelinghuysen Road, Piscataway, NJ 08854, USA
REFERENCE 4 (bases 1 to 2686)
AUTHORS Messing,J.
TITLE Direct Submission
JOURNAL Submitted (12-JAN-2000) Waksman Institute, Rutgers State Univ., 190
Frelinghuysen Road, Piscataway, NJ 08854, USA
REMARK Sequence update by submitter
COMMENT On Jan 12, 2000 this sequence version replaced M77789.1.
FEATURES Location/Qualifiers
source 1..2686
/organism="Cloning vector pUC19"
/mol_type="other DNA"
/strain="ATCC 37254"
/culture_collection="ATCC:37254"
/db_xref="taxon:31851"
misc_feature 1..447
/note="M13mp19"
misc_feature 1..230
/note="Lac-operon"
misc_feature 233..289
/note="polylinker of M13mp19;
HindIII-SphI-PstI-SalI-XbaI-BamHI-SmaI-KpnI-SacI-EcoRI"
misc_feature 290..447
/note="Lac-Operon"
misc_feature complement(448..547)
/note="pBR322"
misc_feature complement(548..684)
/note="pBR322"
misc_feature complement(685..2686)
/note="pBR322"
BASE COUNT 659 a 685 c 675 g 667 t
ORIGIN
1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
61 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
121 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
181 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcttgc
241 atgcctgcag gtcgactcta gaggatcccc gggtaccgag ctcgaattca ctggccgtcg
301 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac
361 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
421 agttgcgcag cctgaatggc gaatggcgcc tgatgcggta ttttctcctt acgcatctgt
481 gcggtatttc acaccgcata tggtgcactc tcagtacaat ctgctctgat gccgcatagt
541 taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc
601 cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt
661 caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg
721 ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc
781 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
841 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
901 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
961 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
1021 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
1081 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
1141 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
1201 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
1261 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
1321 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
1381 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
1441 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
1501 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
1561 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
1621 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
1681 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
1741 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
1801 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
1861 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
1921 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
1981 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
2041 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
2101 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
2161 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
2221 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
2281 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
2341 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
2401 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
2461 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
2521 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
2581 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
2641 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaaga
//