LOCUS YSCCORA 8241 bp DNA linear PLN 06-APR-1996
DEFINITION Yeast COR gene cluster encoding iso-1-cytochrome c (CYC1),
dispensible protein UTR3, osmotic growth protein (OSM1), Gly-tRNA,
and UV-induced damage repair protein (RAD7), complete cds.
ACCESSION M37696 J01319 M12920 M27552
VERSION M37696.1
KEYWORDS COR gene cluster; CYC1 gene; OSM1 gene; RAD7 gene; UTR1 gene; UTR3
gene; UV-induced damage repair protein; dispensible protein;
iso-1-cytochrome c; osmotic growth protein; transfer RNA; transfer
RNA-Gly.
SOURCE Saccharomyces cerevisiae (brewer's yeast)
ORGANISM Saccharomyces cerevisiae
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina;
Saccharomycetes; Saccharomycetales; Saccharomycetaceae;
Saccharomyces.
REFERENCE 1 (bases 249 to 352)
AUTHORS Montgomery,D.L., Hall,B.D., Gillam,S. and Smith,M.
TITLE Identification and isolation of the yeast cytochrome c gene
JOURNAL Cell 14 (3), 673-680 (1978)
PUBMED 210956
REFERENCE 2 (bases 1 to 857)
AUTHORS Smith,M., Leung,D.W., Gillam,S., Astell,C.R., Montgomery,D.L. and
Hall,B.D.
TITLE Sequence of the gene for iso-1-cytochrome c in Saccharomyces
cerevisiae
JOURNAL Cell 16 (4), 753-761 (1979)
PUBMED 222467
REFERENCE 3 (bases 190 to 299)
AUTHORS Stiles,J.I., Szostak,J.W., Young,A.T., Wu,R., Consaul,S. and
Sherman,F.
TITLE DNA sequence of a mutation in the leader region of the yeast
iso-1-cytochrome c mRNA
JOURNAL Cell 25 (1), 277-284 (1981)
PUBMED 6268305
REFERENCE 4 (bases 188 to 280; 576 to 753)
AUTHORS Boss,J.M., Gillam,S., Zitomer,R.S. and Smith,M.
TITLE Sequence of the yeast iso-1-cytochrome c mRNA
JOURNAL J. Biol. Chem. 256 (24), 12958-12961 (1981)
PUBMED 6273415
REFERENCE 5 (bases 1 to 260)
AUTHORS Faye,G., Leung,D.W., Tatchell,K., Hall,B.D. and Smith,M.
TITLE Deletion mapping of sequences essential for in vivo transcription
of the iso-1-cytochrome c gene
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 78 (4), 2258-2262 (1981)
PUBMED 6264471
REFERENCE 6 (bases 576 to 765)
AUTHORS Zaret,K.S. and Sherman,F.
TITLE DNA sequence required for efficient transcription termination in
yeast
JOURNAL Cell 28 (3), 563-573 (1982)
PUBMED 6280875
REFERENCE 7 (sites)
AUTHORS Ernst,J.F., Hampsey,D.M. and Sherman,F.
TITLE DNA sequences of frameshift and other mutations induced by ICR-170
in yeast
JOURNAL Genetics 111 (2), 233-241 (1985)
PUBMED 2414151
REFERENCE 8 (bases 249 to 578)
AUTHORS Hampsey,D.M., Das,G. and Sherman,F.
TITLE Amino acid replacements in yeast iso-1-cytochrome c. Comparison
with the phylogenetic series and the tertiary structure of related
cytochromes c
JOURNAL J. Biol. Chem. 261 (7), 3259-3271 (1986)
PUBMED 3005287
REFERENCE 9 (bases 771 to 8241)
AUTHORS Melnick,L. and Sherman,F.
TITLE Nucleotide sequence of the COR region: a cluster of six genes in
the yeast Saccharomyces cerevisiae
JOURNAL Gene 87 (2), 157-166 (1990)
PUBMED 2158927
COMMENT Original source text: Saccharomyces cerevisiae (clone: pAB40) DNA.
Dr. Graeme A. Reid (ebmo21@castle.edinburgh.ac.uk) writes us about
the possibility of a sequence error, a deletion of a c at position
4617-4618 (cc->ccc), which would extend the coding region to
position 5225 instead of 4683. He made the following
observations: This sequence is homologous to Shewanella
putrefaciens flavocytochrome c (L04283) and other fumarate
reductases. The present GenBank entry indicates that OSM1 encodes a
protein of 301 residues. Extremely close sequence similarity
between OSM1 protein and flavocytochrome c beyond the end point was
found in another reading frame. It is possible that a sequencing
error accounts for this observation, but it has not been checked
experimentally as yet. The putative signal sequence of the
OSM1 protein does not fit the consensus at all well and there is no
evidence to suggest that this is a membrane protein or enters the
secretory pathway. The proposed correction would restore the
sequebnce QxHPTG that is well conserved throughout the Frd family.
with the histidine in the sequence having been identified at the
active site in E.coli frdA (Schryder et al., 1991). Wild-type yeast
cells have two cytochrome c proteins, iso-1 and iso-2, encoded by
the two distinct genetic loci cyc1 and cyc7, respectively. The
iso-1 cytochrome c protein is the predominant cellular cytochrome
c, normally representing about 95% of the material of that kind.
The cyc1-512 mutant has a 38 bp deletion in the 3' nontranslated
region which prevents proper transcription termination. Authors
suggest that polyadenylation may be coupled to transcription
termination in yeast. They also report a consensus sequence from
many yeast genes.
FEATURES Location/Qualifiers
source 1..8241
/organism="Saccharomyces cerevisiae"
/mol_type="genomic DNA"
/db_xref="taxon:4932"
/clone="pAB40"
gene 188..752
/gene="CYC1"
mRNA 188..752
/gene="CYC1"
/citation=[4]
gene 249..578
/gene="COX5b"
/db_xref="SGD:L0000448"
CDS 249..578
/gene="COX5b"
/codon_start=1
/product="iso-1-cytochrome c"
/protein_id="AAB59344.1"
/db_xref="SGD:L0000448"
/translation="MTEFKAGSAKKGATLFKTRCLQCHTVEKGGPHKVGPNLHGIFGR
HSGQAEGYSYTDANIKKNVLWDENNMSEYLTNPKKYIPGTKMAFGGLKKEKDRNDLIT
YLKKACE"
misc_difference 597
/gene="CYC1"
/note="conflict"
/citation=[4]
/replace=""
misc_difference 641..642
/gene="CYC1"
/note="conflict"
/citation=[4]
/replace=""
variation 708..745
/gene="CYC1"
/note="cyc1 deletion in mutant strain cyc1-512 [6]"
gene 2909..3316
/gene="UTR3"
/db_xref="SGD:L0002730"
CDS 2909..3316
/gene="UTR3"
/codon_start=1
/product="dispensable protein"
/protein_id="AAB59345.1"
/db_xref="SGD:L0002730"
/translation="MEKKTKRKRLEDSHVLMNSGKLINGKRYFGRALELPEVKEWLKQ
SQRQNDGGSINTKCIPKDRNDFYYHGKVTAALTEFEANWTSILKAHYNVPVNEDEEEM
SRQTQEIHVPTLADMEHWLVQRRKKKLMDELNL"
gene 3778..4683
/gene="OSM1"
/db_xref="SGD:L0001317"
CDS 3778..4683
/gene="OSM1"
/note="precursor"
/codon_start=1
/product="osmotic growth protein"
/protein_id="AAB59346.1"
/db_xref="SGD:L0001317"
/translation="MIRSVRRVFIYVSIFVLIIVLKRTLSGTDQTSMKQPVVVIGSGL
AGLTTSNRLISKYRIPVVLLDKAASIGGNSIKASSGINGAHTDTQQNLKVMDTPELFL
KDTFDSAKGRGVPSLMDKLTKESKSAIRWLQTEFDLKLDLLAQLGGHSVPRTHRSSGK
LPPGFEIVQARLSKKLKDISSKDSNLVQIMLEVVDIELDNQGHVTGVVYMDENGNRKI
MKSHHVVFCSGGFGYSKEMLKEYSPNLIHLPTTNGKQTTGDGQKILSKLGAELIDMDQ
VQVHLPASLIQMTVKITGSFWLQRH"
sig_peptide 3778..3840
/gene="OSM1"
mat_peptide 3841..4680
/gene="OSM1"
/product="osmotic growth protein"
gene 5743..5811
/gene="tRNA-Gly"
tRNA 5743..5811
/gene="tRNA-Gly"
/product="tRNA-Gly"
gene 5976..7673
/gene="RAD7"
/db_xref="SGD:L0001561"
CDS 5976..7673
/gene="RAD7"
/note="UV-induced"
/codon_start=1
/product="damage-repair protein"
/protein_id="AAB59347.1"
/db_xref="SGD:L0001561"
/translation="MYRSRNRPKRGGENEVKGPNSALTQFLREEGISAENIKQKWYQR
QSKKQEDATDEKKGKAEDDSFTAEISRVVEDEEIDEIGTGSGTETERAQVSYDARMKL
VPADSDEEEYETSHISDTPVSLSSANDRESLTKKRQNTAKIIQNRRRKRKRAADLLDR
RVNKVSSLQSLCITKISENISKWQKEADESSKLVFNKLRDVLGGVSTANLNNLAKALS
KNRALNDHTLQLFLKTDLKRLTFSDCSKISFDGYKTLAIFSPHLTELSLQMCGQLNHE
SFVYIAEKLPNLKSLNLDGPFLINEDTWEKFFVIMKGRLEEFHISNTHRFTDKSLSNL
LINCGSTLVSLGLSRLDSISNYALLPQYLVNDEFHSLCIEYPFNEEDVNDEIIINLLG
QIGRTLRKLVLNGCIDLTDSMIINGLTAFIPEKCPLEVLSLEESDQITTDSLSYFFSK
VELNNLIECSFRRCLQLGDMAIIELLLNGARDSLRSLNLNSLKELTKEAFVALRPPNL
TYLDLGFVRCVDDSVIQMLGEQNPNLTVIDVFGDNLVTEKATMRPGLTLIGRQSDSI"
BASE COUNT 2624 a 1530 c 1645 g 2441 t
ORIGIN
1 ctcgagcaga tccgccaggc gtgtatatag cgtggatggc caggcaactt tagtgctgac
61 acatacaggc atatatatat gtgtgcgacg acacatgatc atatggcatg catgtgctct
121 gtatgtatat aaaactcttg ttttcttctt ttctctaaat attctttcct tatacattag
181 gtcctttgta gcataaatta ctatacttct atagacacgc aaacacaaat acacacacta
241 aattaataat gactgaattc aaggccggtt ctgctaagaa aggtgctaca cttttcaaga
301 ctagatgtct acaatgccac accgtggaaa agggtggccc acataaggtt ggtccaaact
361 tgcatggtat ctttggcaga cactctggtc aagctgaagg gtattcgtac acagatgcca
421 atatcaagaa aaacgtgttg tgggacgaaa ataacatgtc agagtacttg actaacccaa
481 agaaatatat tcctggtacc aagatggcct ttggtgggtt gaagaaggaa aaagacagaa
541 acgacttaat tacctacttg aaaaaagcct gtgagtaaac aggccccttt tcctttgtcg
601 atatcatgta attagttatg tcacgcttac attcacgccc tccccccaca tccgctctaa
661 ccgaaaagga aggagttaga caacctgaag tctaggtccc tatttatttt tttatagtta
721 tgttagtatt aagaacgtta tttatatttc aaatttttct tttttttctg tacagacgcg
781 tgtacgcatg taacattata ctgaaaacct tgcttgagaa ggttttggga cgctcgaagg
841 ctttaatttg caagcttcgc agttttcact ctcatcgtcg ctctcatcat cgcttccgtt
901 gttgttttcc ttaatagcgt ctgcttccag agagtattta tctcttatta cctctaaagg
961 ttctgcttga tttctgactt tgttcgcctc atgtgcatat ttttcttggt tctttgggac
1021 aaaatatgcg taaaggactt ttgttgttcc ctcacattcc cagtttagtt gtcgactgat
1081 actgttaata aactcatcgg gcgaggcttc cacggttgga aaagcatatg ggctggcgca
1141 tatggttata aaatcacctt tttgcaattc aattctatgt ttcccatcaa aagccgccca
1201 tgctggagcc cttgacttca tcgagacttt cacttttaaa tttatacttt ctggtaagat
1261 gatgggtctg aaactcaatg catgtggaca aatgggtgtt aaagcgattg cattgacggt
1321 tgggcatacc aatgacccac ctgcactcaa agaataggcc gtggacccag tcggagtagc
1381 agcaatcagt ccgtccgcct gcgcaacggt cattaatgag ccgtcaccat acaattctaa
1441 catggataga aaaggacttg gaccacgatc gatggtcact tcgttcaaaa tgtggtgtgt
1501 gcttagtttt tccaccacac atattttctt ccccgtgttt gggtctactt cagggcggtg
1561 tctacgataa attgtgcact ccaacctcaa ccgtaaattt gtcttgattt tatgattcat
1621 aatccgaggt aaatcctccc tgaaatgttc aaacttaaaa tttgttaaaa atcctagaga
1681 ccctaatgaa aacgacataa cgggtggtac atgtctctga aaaatggaac ttacaaaaag
1741 aacagtaccg tcgccaccca aagtcactac caaatcgaag aaaaacatca tgttccctga
1801 tgaaatcctt tgtccaatac ttgatccttg attctctaca tttactatct tcacataact
1861 cgccagcgcg aattttttgc tgtttttcaa ttcggaatcc acataaacag tcacacgtgg
1921 aaaatgtacc aaaacccatt ctaccaactc tcttgttaag aaatacagtg agacatcgtt
1981 gagtttcgta acaatcatca aattttccac atccagttcc actttggtat tagatatatc
2041 tttacttaac attcttacac catacgccgt ggaggcatac tgaaagtgtg tattactact
2101 cttagaactt cctcttgttc cgttaatgct cgtaccaccg ccactgtttg cattgccgtt
2161 cacgagagat gaatctttat tcaacaggga gcttctgcgc gagctgcttt cacttgatat
2221 tttcctcaac atttctttgg cgttgtcgat atcctgagtt ctatcaattt gctcattgtt
2281 catcatggcc ttcttcatca agttattatt gttgttatga tgatcatttc gaccatcttc
2341 ctcatttacc catttatcta cgccattatt catgtcattc tccttcatct caagagcttt
2401 tactctactt atttagggag cctattgtat ttttttttgg cttacgatca cgatgtcaat
2461 gatcatttgc cctattatac ctttttctcc gttctctttg cgtagtgcgg tgaaataatt
2521 tcaggatgca tgaaagagca aataaaagtg tcacaccgtt atatcgcaag gcgcaccaca
2581 tcagtaatca gatacctgtg cattcaaaat gagtagaaat gtagataagg ccaactcagt
2641 tctggtacga tttcaagagc agcaagcaga gtcggcaggt ggatacaagg attattcacg
2701 ttaccagagg cccaggagcg tgtctaaggt aaaatccata aaagaggcca acgaatggaa
2761 gcgacaagta agtaaagaga taaaacaaaa aagcacaaga atatatgatc cgtctttaaa
2821 tgaaatgcag attgcggaac ttaacgacga acttaataat cttttcaaac aatggaagag
2881 atggcagtgg cacattgacc atacacttat ggaaaaaaaa accaagagga aaaggttaga
2941 agacagtcat gtgctgatga attcaggaaa gctgataaat ggtaagagat attttggaag
3001 agccctggaa ttgcctgaag taaaagaatg gctcaagcag tctcaaaggc agaatgatgg
3061 gggttctata aacaccaaat gcataccgaa ggatagaaat gatttttatt accatggcaa
3121 agtcacagcc gccttaaccg aatttgaggc taactggaca tccattttga aagcacatta
3181 taatgtgcct gtgaatgaag atgaagaaga aatgtcaagg cagacacaag aaatccatgt
3241 accaactctg gcagatatgg agcattggtt agtgcaaaga agaaaaaaga aactaatgga
3301 tgaacttaac ctttagctat ggaaaattat tcaaacattt agaagaggca ctatttcgaa
3361 tgaccatcac aagcattata atcttcaaac atgtccgagc tgcagatatg aagtaaaata
3421 gtgatcggga ctcatttttt tttttcttct atgatatcat attgtcccgt tcgttattaa
3481 atgtaattct tccaccgtac tagttatcga aggcaagatc tcctttttac gcttaggtag
3541 attacataga tttcataatg tacaccaata caaatgtatc ttatacactc gtcaaatctt
3601 atatgaatca atgcaggagt agctctcgtt ttctttttac gagctacttg aaaaaaaaaa
3661 aactagcgaa aatcctttgc ttagaaatcc agatttaaaa ccaaaaccgc cgaaaaggga
3721 aagattttat gataggaaat accttaataa ttctatatca tcccgagtct taggaaaatg
3781 attagatctg tgagaagggt tttcatttac gtctcaatat tcgtattgat aatagttttg
3841 aaaagaacat taagtggcac agatcaaacg tcaatgaaac aaccagtggt ggtcattggc
3901 tctggtttgg caggcttaac cacaagtaat cgtctcatta gtaaatacag aattcctgtt
3961 gtgcttttgg ataaggcggc ttctattggt gggaattcta taaaggcttc tagtggtatt
4021 aatggtgctc acacagacac tcaacaaaat ttaaaggtaa tggacactcc cgaattgttt
4081 ttgaaagata ctttcgattc ggctaaaggc agaggggttc catcactgat ggataagttg
4141 actaaggaat ccaagagtgc tatcaggtgg ttgcaaacag aattcgattt gaaattagac
4201 ctccttgcgc aattgggcgg tcactctgtt ccaaggaccc atagatcttc tggcaaatta
4261 ccaccaggtt ttgaaatcgt gcaagcgcgt ttatcaaaaa aactaaagga tatctcttcc
4321 aaagattcca atctcgtgca gattatgctt gaagtagtgg atatcgagct tgataatcaa
4381 ggtcatgtta ctggtgtagt atatatggac gagaacggaa accgtaaaat catgaagtca
4441 caccatgtcg tgttttgctc aggtggattt ggttactcta aggaaatgtt gaaagagtac
4501 tcaccaaatt tgattcactt gccaactact aatggcaaac agactacagg tgatggtcaa
4561 aaaatccttt caaagttggg tgccgaattg attgatatgg atcaagtgca ggtacaccta
4621 ccggcttcat tgatccaaat gaccgtgaaa ataactggaa gtttttggct gcagaggcat
4681 tgaggggttt aggcggcatc ttattgcatc ccaccactgg aagaaggttt acaaatgaat
4741 tgagcaccag agatacagta accatggaaa tacagtctaa atgtccgaaa aatgataata
4801 gagcactttt ggtaatgagc gacaaagtct acgagaacta tacgaataac ataaactttt
4861 atatgtccaa aaacttaatc aaaaaagtgt caatcaacga tctgatccga caatatgacc
4921 tacaaactac agcttctgaa ctggtaactg aactgaagag ctattccgat gttaatacta
4981 aggatacgtt tgataggcca ttgattatca atgcctttga taaagatatt tcgactgaat
5041 caactgttta tgttggggaa gttacaccag ttgttcattt cacaatgagt ggtgtgaaaa
5101 ttaatgagaa atctcaggta attaagaaaa attcggaaac gcttctatct aatgggatat
5161 ttgctgctgg tgaagtttcg ggtggtgttc atggagccaa cagattgggt ggatctagtt
5221 ttgtttagag tgtgttgtct ttggaaagac agctgcggat aacatagcaa aattgtactg
5281 agaatttata gggaaataaa agatattttt gcaacgtact ttacatttca ataattaatg
5341 cgacatgata ggatatactg tttgctattt ctttccgtaa cgatatacga ttttttcttt
5401 tctttggagt tccaaatgaa gaatatatct tgttgggaaa agctatagta ttaggtttac
5461 agaatatggt agaggtttct ccccgacgat ataggaatct acaagagaga gcatgattct
5521 acacgataat actgcaattt cttctttgcg ttgaagtgtc accattgcct atccttttac
5581 attatcaatg tcaatgttcc ctcttctaac aaactgaatg acttttctac acgattcata
5641 ttatcttctt gcgcagtaaa ttatgttaac gtaatagtag ggtctcttgt gcttcattat
5701 tttgtaactg tacacataat tgacacgttt aacaattagt gagcgcaatg gtttagtggt
5761 aaaatccaac gttgccatcg ttgggccccc ggttcgattc cgggcttgcg cagtttcttt
5821 tctttttttc tggaaaataa gaaaacttat ttatggaagc aaaaatggaa taaaggattg
5881 ggacgaagtt agtgaaaaaa aactgaaata gtcctagagt aactccgaag tgtctttgta
5941 taagctatta tctagaaatc acgagagggg aagaaatgta tcgcagtaga aaccgaccaa
6001 aaagaggtgg agaaaatgaa gttaagggac caaattctgc cttgactcaa tttttaagag
6061 aagaagggat cagtgctgaa aatatcaaac aaaaatggta ccagcgacag tcgaagaagc
6121 aagaagatgc aacagacgaa aaaaaaggta aagcggagga tgatagcttt actgccgaga
6181 tatctcgagt agttgaagat gaagaaattg atgaaattgg aacaggtagt ggtaccgaga
6241 cagaaagagc tcaggtttcc tacgatgcca ggatgaaatt agtccctgct gatagtgatg
6301 aagaagaata tgaaactagc cacatttctg acacgccagt cagtttaagt tcggctaatg
6361 accgggaatc attgactaaa aaaaggcaaa atactgcaaa aattatccaa aatcgtcgca
6421 gaaagcgtaa aagagcagct gacctattgg atagacgcgt caacaaagta tccagcttac
6481 aaagtctttg tattacgaaa attagtgaaa atatatccaa gtggcaaaaa gaggctgatg
6541 aatcatcaaa gttggtattt aacaaattga gagatgtcct tggtggcgta tcaaccgcta
6601 atttgaataa tttggcaaaa gcactatcga agaatagggc cctgaatgat catactttgc
6661 aacttttctt gaagacagat ctaaaaaggt taactttcag cgattgttct aaaatttcat
6721 ttgatggtta caaaacgcta gccatttttt cgccacacct aaccgaatta tccctacaaa
6781 tgtgtgggca gttgaaccat gaatcattcg tttacattgc tgaaaagcta ccgaacttga
6841 aatcgctgaa tttagatgga ccatttctga tcaacgagga cacatgggag aagttctttg
6901 taataatgaa aggtagatta gaagagttcc acatttctaa tacgcaccgc ttcaccgaca
6961 aatcattatc taatttattg atcaactgcg gttctacctt ggtatcctta gggttatcca
7021 gactagattc tatatcaaat tacgctttat taccgcagta cctagtcaac gacgaatttc
7081 acagtctctg tattgaatat ccattcaatg aagaggatgt taacgacgag atcatcataa
7141 atctgctagg tcaaatcggg cgcacgttac gtaaattggt tttgaatggc tgtattgact
7201 tgacagattc aatgataatc aatggtctga ctgcattcat tcctgagaaa tgtccattgg
7261 aggtattgag cttggaagaa tcagatcaga tcactacaga ttcactgtcg tactttttca
7321 gcaaagtaga actgaataat ttgattgaat gcagctttag aagatgtcta caattgggcg
7381 atatggcaat tatagagcta ttgcttaacg gagcaagaga tagtctgaga agcttaaatc
7441 tcaattcatt aaaagagcta actaaggagg catttgtggc gttacgtcct cctaatctga
7501 cgtatcttga tcttggtttt gtacgttgtg ttgatgactc ggtgattcaa atgttgggtg
7561 agcaaaatcc gaatttaact gtaattgatg ttttcggaga caatttggtt actgaaaagg
7621 ccacaatgag gcctggactt acgttgatag ggagacagag tgacagtata taataaaata
7681 aagacatata aaacgtgtat tagatagaaa acggaaaatt gactaatgtt ttattcctat
7741 tatacccaga tcgtcaaatg ttgcctttat tttctattga agggatttta tggcatctcg
7801 cgaataataa acaaacaata atagtaaaaa aaaaagtgta aacttatgta ttcagcgcag
7861 ataaaaggaa aaaaacgaga agtttgacgt aaagaaaaaa gttcgaaaac ttttgtagca
7921 gttgaaagtt ttgtatgcta tgtcaattag gcctctcacg ttaaacggtt tagatgagcc
7981 agaaacctct tttgaagaac tgaatacaac tctacctcgc tttcaatccc atgaaacatt
8041 aactttggna gaaaacgtgc caccattgag tacatcaact tatataccgc ctccatcctc
8101 ggtaggtgac ttctgacact ggcgacagta ttttccaaca gtaccacgcg ctttttggtg
8161 ctaataatgc aagcagaacg atgatcagga catggaggta gaccacggat gatgagtttc
8221 taaatgactt ccacggaatt c
//