LOCUS SYNMMLPLN3 6620 bp DNA linear SYN 22-SEP-2008
DEFINITION Retroviral vector pLNCX, complete sequence.
ACCESSION M28247
VERSION M28247.1
KEYWORDS genome; neomycin phosphotransferase; retroviral vector.
SOURCE Retroviral vector pLNCX
ORGANISM Retroviral vector pLNCX
other sequences; artificial sequences; vectors.
REFERENCE 3 (bases 1 to 6620)
AUTHORS Miller,A.D.
TITLE Direct Submission
JOURNAL Submitted (22-SEP-1989) A.D. Miller, Program in Molecular Medicine,
Fred Hutchinson Cancer Research Center, Seattle, WA 98104, USA
REFERENCE 4 (bases 1 to 6620)
AUTHORS Miller,A.D. and Rosman,G.J.
TITLE Improved retroviral vectors for gene transfer and expression
JOURNAL BioTechniques 7 (9), 980-982 (1989)
PUBMED 2631796
FEATURES Location/Qualifiers
source 1..6620
/organism="Retroviral vector pLNCX"
/mol_type="other DNA"
/db_xref="taxon:147133"
/note="derived from Moloney murine leukemia virus"
misc_recomb 144^145
/note="mouse DNA end-Mo-MuSV DNA start"
repeat_region 145..733
/note="Mo-MuSV 5' long terminal repeat"
/rpt_type=long_terminal_repeat
regulatory 803..1612
/regulatory_class="other"
/note="extended packaging signal"
misc_recomb 1133^1134
/note="Mo-MuSV DNA end-Mo-MuLV DNA start"
variation 1193..1195
/note="gag ATG start codon to TAG stop codon"
misc_recomb 1616^1617
/note="Mo-MuLV DNA end-Tn5 DNA start"
CDS 1656..2450
/codon_start=1
/product="neomycin phosphotransferase"
/protein_id="AAA72064.1"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
misc_recomb 2799^2800
/note="Tn5 DNA end-human cytomegalovirus DNA start"
regulatory 2800..3617
/regulatory_class="other"
/note="human cytomegalovirus immediate early promoter"
misc_recomb 3617^3618
/note="human cytomegalovirus DNA end-Mo-MuLV DNA start"
repeat_region 3666..4259
/note="Mo-MuLV 3' long terminal repeat"
/rpt_type=long_terminal_repeat
misc_recomb 4328^4329
/note="Mo-MuLV DNA end-plasmid pBR322 DNA start"
BASE COUNT 1528 a 1807 c 1692 g 1593 t
ORIGIN
1 gaattcatac cagatcaccg aaaactgtcc tccaaatgtg tccccctcac actcccaaat
61 tcgcgggctt ctgcctctta gaccactcta ccctattccc cacactcacc ggagccaaag
121 ccgcggccct tccgtttctt tgcttttgaa agaccccacc cgtaggtggc aagctagctt
181 aagtaacgcc actttgcaag gcatggaaaa atacataact gagaatagaa aagttcagat
241 caaggtcagg aacaaagaaa cagctgaata ccaaacagga tatctgtggt aagcggttcc
301 tgccccggct cagggccaag aacagatgag acagctgagt gatgggccaa acaggatatc
361 tgtggtaagc agttcctgcc ccggctcggg gccaagaaca gatggtcccc agatgcggtc
421 cagccctcag cagtttctag tgaatcatca gatgtttcca gggtgcccca aggacctgaa
481 aatgaccctg taccttattt gaactaacca atcagttcgc ttctcgcttc tgttcgcgcg
541 cttccgctct ccgagctcaa taaaagagcc cacaacccct cactcggcgc gccagtcttc
601 cgatagactg cgtcgcccgg gtacccgtat tcccaataaa gcctcttgct gtttgcatcc
661 gaatcgtggt ctcgctgttc cttgggaggg tctcctctga gtgattgact acccacgacg
721 ggggtctttc atttgggggc tcgtccggga tttggagacc cctgcccagg gaccaccgac
781 ccaccaccgg gaggtaagct ggccagcaac ttatctgtgt ctgtccgatt gtctagtgtc
841 tatgtttgat gttatgcgcc tgcgtctgta ctagttagct aactagctct gtatctggcg
901 gacccgtggt ggaactgacg agttctgaac acccggccgc aaccctggga gacgtcccag
961 ggactttggg ggccgttttt gtggcccgac ctgaggaagg gagtcgatgt ggaatccgac
1021 cccgtcagga tatgtggttc tggtaggaga cgagaaccta aaacagttcc cgcctccgtc
1081 tgaatttttg ctttcggttt ggaaccgaag ccgcgcgtct tgtctgctgc agcgctgcag
1141 catcgttctg tgttgtctct gtctgactgt gtttctgtat ttgtctgaaa attagggcca
1201 gactgttacc actcccttaa gtttgacctt aggtcactgg aaagatgtcg agcggatcgc
1261 tcacaaccag tcggtagatg tcaagaagag acgttgggtt accttctgct ctgcagaatg
1321 gccaaccttt aacgtcggat ggccgcgaga cggcaccttt aaccgagacc tcatcaccca
1381 ggttaagatc aaggtctttt cacctggccc gcatggacac ccagaccagg tcccctacat
1441 cgtgacctgg gaagccttgg cttttgaccc ccctccctgg gtcaagccct ttgtacaccc
1501 taagcctccg cctcctcttc ctccatccgc cccgtctctc ccccttgaac ctcctcgttc
1561 gaccccgcct cgatcctccc tttatccagc cctcactcct tctctaggcg ccggaattcc
1621 gatctgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg
1681 caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa
1741 tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg
1801 tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt
1861 ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa
1921 gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc
1981 ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg
2041 ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg
2101 aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg
2161 aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg
2221 gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact
2281 gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg
2341 ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc
2401 ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct
2461 ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac
2521 cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat
2581 cctccagcgc ggggatctca tgctggagtt cttcgcccac cccgggctcg atcccctcgc
2641 gagttggttc agctgctgcc tgaggctgga cgacctcgcg gagttctacc ggcagtgcaa
2701 atccgtcggc atccaggaaa ccagcagcgg ctatccgcgc atccatgccc ccgaactgca
2761 ggagtgggga ggcacgatgg ccgctttggt cgaggcggat ccggccatta gccatattat
2821 tcattggtta tatagcataa atcaatattg gctattggcc attgcatacg ttgtatccat
2881 atcataatat gtacatttat attggctcat gtccaacatt accgccatgt tgacattgat
2941 tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg
3001 agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc
3061 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt
3121 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc
3181 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
3241 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
3301 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
3361 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
3421 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
3481 ggcatgtacg gtgggaggtc tatataagca gagctcgttt agtgaaccgt cagatcgcct
3541 ggagacgcca tccacgctgt tttgacctcc atagaagaca ccgggaccga tccagcctcc
3601 gcggccccaa gcttgttaac atcgataaaa taaaagattt tatttagtct ccagaaaaag
3661 gggggaatga aagaccccac ctgtaggttt ggcaagctag cttaagtaac gccattttgc
3721 aaggcatgga aaaatacata actgagaata gagaagttca gatcaaggtc aggaacagat
3781 ggaacagctg aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca
3841 gggccaagaa cagatggaac agctgaatat gggccaaaca ggatatctgt ggtaagcagt
3901 tcctgccccg gctcagggcc aagaacagat ggtccccaga tgcggtccag ccctcagcag
3961 tttctagaga accatcagat gtttccaggg tgccccaagg acctgaaatg accctgtgcc
4021 ttatttgaac taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc tgctccccga
4081 gctcaataaa agagcccaca acccctcact cggggcgcca gtcctccgat tgactgagtc
4141 gcccgggtac ccgtgtatcc aataaaccct cttgcagttg catccgactt gtggtctcgc
4201 tgttccttgg gagggtctcc tctgagtgat tgactacccg tcagcggggg tctttcattt
4261 gggggctcgt ccgggatcgg gagacccctg cccagggacc accgacccac caccgggagg
4321 taagctggct gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc
4381 ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc
4441 gcgtcagcgg gtgttggcgg gtgtcggggc gcagccatga cccagtcacg tagcgatagc
4501 ggagtgtata ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata
4561 tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg
4621 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
4681 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
4741 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
4801 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
4861 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
4921 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
4981 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
5041 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
5101 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
5161 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
5221 acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
5281 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
5341 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
5401 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
5461 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
5521 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
5581 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
5641 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
5701 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
5761 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
5821 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct gcaggcatcg
5881 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
5941 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
6001 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
6061 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
6121 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca acacgggata
6181 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
6241 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
6301 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
6361 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
6421 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
6481 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
6541 cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca
6601 cgaggccctt tcgtcttcaa
//