LOCUS SYNTRC99A 4176 bp DNA linear SYN 29-JUN-1995
DEFINITION Cloning vector pTrc99A, complete sequence.
ACCESSION M22744
VERSION M22744.1
KEYWORDS cloning vector.
SOURCE Cloning vector pTrc99A
ORGANISM Cloning vector pTrc99A
other sequences; artificial sequences; vectors.
REFERENCE 1 (sites)
AUTHORS Amann,E. and Brosius,J.
TITLE 'ATG vectors' for regulated high-level expression of cloned genes
in Escherichia coli
JOURNAL Gene 40 (2-3), 183-190 (1985)
PUBMED 3007288
REFERENCE 2 (bases 1 to 4176)
AUTHORS Amann,E., Ochs,B. and Abel,K.J.
TITLE Tightly regulated tac promoter vectors useful for the expression of
unfused and fused proteins in Escherichia coli
JOURNAL Gene 69 (2), 301-315 (1988)
PUBMED 3069586
FEATURES Location/Qualifiers
source 1..4176
/organism="Cloning vector pTrc99A"
/mol_type="genomic DNA"
/db_xref="taxon:40992"
/lab_host="Escherichia coli"
/note="derived from pKK233-2"
misc_feature 1..17
/note="derived from cloning vector pBR322"
regulatory 18..263
/regulatory_class="promoter"
/note="trc promoter from pKK233-2"
/citation=[1]
misc_feature 264..270
/note="NcoI/EcoRI linker"
misc_feature 271..321
/note="derived from cloning vector pUC18"
misc_feature 329..753
/note="5S RNA, T1, T2, rrnB"
misc_feature 754..2932
/note="derived from cloning vector pBR322"
misc_feature 2933..2940
/note="BglII linker"
misc_feature 2933..2938
/note="BglII linker"
misc_feature 2939..2945
/note="EcoRI linker"
misc_feature 2946..2976
/note="derived from plasmid RP4"
misc_feature 2977..4163
/note="lacI-q region"
misc_feature 4164..4170
/note="EcoRI linker"
misc_feature 4171..4176
/note="BglII linker"
BASE COUNT 1009 a 1059 c 1118 g 990 t
ORIGIN
1 gtttgacagc ttatcatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc
61 ggaagctgtg gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc
121 gcactcccgt tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc
181 tgaaatgagc tgttgacaat taatcatccg gctcgtataa tgtgtggaat tgtgagcgga
241 taacaatttc acacaggaaa cagaccatgg aattcgagct cggtacccgg ggatcctcta
301 gagtcgacct gcaggcatgc aagcttggct gttttggcgg atgagagaag attttcagcc
361 tgatacagat taaatcagaa cgcagaagcg gtctgataaa acagaatttg cctggcggca
421 gtagcgcggt ggtcccacct gaccccatgc cgaactcaga agtgaaacgc cgtagcgccg
481 atggtagtgt ggggtctccc catgcgagag tagggaactg ccaggcatca aataaaacga
541 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc
601 ctgagtagga caaatccgcc gggagcggat ttgaacgttg cgaagcaacg gcccggaggg
661 tggcgggcag gacgcccgcc ataaactgcc aggcatcaaa ttaagcagaa ggccatcctg
721 acggatggcc tttttgcgtt tctacaaact ctttttgttt atttttctaa atacattcaa
781 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
841 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
901 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
961 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
1021 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
1081 tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
1141 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
1201 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
1261 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
1321 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
1381 cgatgcctac agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
1441 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
1501 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
1561 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
1621 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
1681 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
1741 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
1801 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
1861 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
1921 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
1981 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt
2041 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
2101 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
2161 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
2221 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
2281 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
2341 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
2401 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
2461 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
2521 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
2581 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
2641 cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca
2701 tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc
2761 gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc
2821 gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg
2881 gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agcagatcaa
2941 ttcgcgcgcg aaggcgaagc ggcatgcatt tacgttgaca ccatcgaatg gtgcaaaacc
3001 tttcgcggta tggcatgata gcgcccggaa gagagtcaat tcagggtggt gaatgtgaaa
3061 ccagtaacgt tatacgatgt cgcagagtat gccggtgtct cttatcagac cgtttcccgc
3121 gtggtgaacc aggccagcca cgtttctgcg aaaacgcggg aaaaagtgga agcggcgatg
3181 gcggagctga attacattcc caaccgcgtg gcacaacaac tggcgggcaa acagtcgttg
3241 ctgattggcg ttgccacctc cagtctggcc ctgcacgcgc cgtcgcaaat tgtcgcggcg
3301 attaaatctc gcgccgatca actgggtgcc agcgtggtgg tgtcgatggt agaacgaagc
3361 ggcgtcgaag cctgtaaagc ggcggtgcac aatcttctcg cgcaacgcgt cagtgggctg
3421 atcattaact atccgctgga tgaccaggat gccattgctg tggaagctgc ctgcactaat
3481 gttccggcgt tatttcttga tgtctctgac cagacaccca tcaacagtat tattttctcc
3541 catgaagacg gtacgcgact gggcgtggag catctggtcg cattgggtca ccagcaaatc
3601 gcgctgttag cgggcccatt aagttctgtc tcggcgcgtc tgcgtctggc tggctggcat
3661 aaatatctca ctcgcaatca aattcagccg atagcggaac gggaaggcga ctggagtgcc
3721 atgtccggtt ttcaacaaac catgcaaatg ctgaatgagg gcatcgttcc cactgcgatg
3781 ctggttgcca acgatcagat ggcgctgggc gcaatgcgcg ccattaccga gtccgggctg
3841 cgcgttggtg cggatatctc ggtagtggga tacgacgata ccgaagacag ctcatgttat
3901 atcccgccgt caaccaccat caaacaggat tttcgcctgc tggggcaaac cagcgtggac
3961 cgcttgctgc aactctctca gggccaggcg gtgaagggca atcagctgtt gcccgtctca
4021 ctggtgaaaa gaaaaaccac cctggcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
4081 gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg
4141 caacgcaatt aatgtgagtt agcgcgaatt gatctg
//