LOCUS EMEAMDS 3430 bp DNA linear PLN 27-APR-1993
DEFINITION A.nidulans amdS gene encoding an acetamidase enzyme, complete cds.
ACCESSION M16371
VERSION M16371.1
KEYWORDS acetamidase.
SOURCE Aspergillus nidulans
ORGANISM Aspergillus nidulans
Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina;
Eurotiomycetes; Eurotiomycetidae; Eurotiales; Aspergillaceae;
Aspergillus; Aspergillus subgen. Nidulantes.
REFERENCE 1 (bases 1 to 3430)
AUTHORS Corrick,C.M., Twomey,A.P. and Hynes,M.J.
TITLE The nucleotide sequence of the amdS gene of Aspergillus nidulans
and the molecular characterization of 5' mutations
JOURNAL Gene 53 (1), 63-71 (1987)
PUBMED 3036667
COMMENT Original source text: A.nidulans (strain Glasgow) DNA.
Draft entry and printed copy of sequence [1] kindly provided by
M.J.Hynes, 13-JUL-1987.
FEATURES Location/Qualifiers
source 1..3430
/organism="Aspergillus nidulans"
/mol_type="genomic DNA"
/db_xref="taxon:162425"
/note="teleomorph: Emericella nidulans"
prim_transcript 1007..>3430
/note="amdS mRNA and intron"
CDS join(1048..1311,1392..1532,1593..2013,2127..2947)
/note="acetamidase enzyme"
/codon_start=1
/protein_id="AAA33295.1"
/translation="MPQSWEELAADKRARLAKTIPDEWKVQTLPAEDSVIDFPKKSGI
LSEAELKITEASAADLVSKLAAGELTSVEVTLAFCKRAAIAQQLTNCAHEFFPDAALA
QARELDEYYAKHKRPVGPLHGLPISLKDQLRVKGYETSMGYISWLNKYDEGDSVLTTM
LRKAGAVFYVKTSVPQTLMVCETVNNIIGRTVNPRNKNWSCGGSSGGEGAIVGIRGGV
IGVGTDIGGSIRVPAAFNFLYGLRPSHGRLPYAKMANSMEGQETVHSVVGPITHSVED
LRLFTKSVLGQEPWKYDSKVIPMPWRQSESDIIASKIKNGGLNIGYYNFDGNVLPHPP
ILRGVETTVAALAKAGHTVTPWTPYKHDFGHDLISHIYAADGSADVMRDISASGEPAI
PNIKDLLNPNIKAVNMNELWDTHLQKWNYQMEYLEKWREAEEKAGKELDAIIAPITPT
AAVRHDQFRYYGYASVINLLDFTSVVVPVTFADKNIDKKNESFKAVSELDALVQEEYD
PEAYHGAPVAVQVIGRRLSEERTLAIAEEVGKLLGNVVTP"
exon <1048..1311
/note="acetamidase enzyme"
/number=1
intron 1312..1391
/note="amdS intron A"
exon 1392..1532
/number=2
intron 1533..1592
/note="amdS intron B"
exon 1593..2013
/number=3
intron 2014..2126
/note="amdS intron C"
exon 2127..>2947
/note="acetamidase enzyme"
/number=4
BASE COUNT 868 a 910 c 871 g 781 t
ORIGIN 235 bp upstream of AccI site; linkage group III.
1 ggccctgaag gtcggatgta cgacgtatat ccatctttaa ctagtcatca ttggataggc
61 agattactca gcctgaatga catcaacatg ttacccatga tacaataggt cacacaaaca
121 agcgctaaga tgcacttggt atgacaagcc cagtagtccg tttcaaaaga cctagatgat
181 gaactacaac atgaggtgtt gcctcctgat ccagtccaac tgcaaacgct gatgtatact
241 caatcaagcc tgatgtaaat gctgcgactc gattcgctgg atatgaagat caaagagagc
301 tctgatgggt ccaatatagc cgggttttgt taggacagtc caccacaccg atattagaat
361 tggtcaagca ccttatcatt tcatagagat tgcggtttct agatctacgc caggaccgag
421 caagcccaga tgagaaccga cgcagatttc cttggcacct gttgcttcag ctgaatcctg
481 gcaatacgag atacctgctt tgaatatttt gaatagctcg cccgctggag agcatcctga
541 atgcaagtaa caaccgtaga ggctgacacg gcaggtgttg ctagggagcg tcgtgttcta
601 caaggccaga cgtcttcgcg gttgatatat atgtatgttt gactgcaggc tgctcagcga
661 cgacagtcaa gttcgccctc gctgcttgtg caataatcgc agtggggaag ccacaccgtg
721 actcccatct ttcagtaaag ctctgttggt gtttatcagc aatacacgta atttaaactc
781 gttagcatgg ggctgatagc ttaattaccg tttaccagtg ccgcggttct gcagctttcc
841 ttggcccgta aaattcggcg aagccagcca atcaccagct aggcaccagc taaaccctat
901 aattagtctc ttatcaacac catccgctcc cccgggatca atgaggagaa tgagggggat
961 gcggggctaa agaagcctac ataaccctca tgccaactcc cagtttacac tcgtcgagcc
1021 aacatcctga ctataagcta acacagaatg cctcaatcct gggaagaact ggccgctgat
1081 aagcgcgccc gcctcgcaaa aaccatccct gatgaatgga aagtccagac gctgcctgcg
1141 gaagacagcg ttattgattt cccaaagaaa tcggggatcc tttcagaggc cgaactgaag
1201 atcacagagg cctccgctgc agatcttgtg tccaagctgg cggccggaga gttgacctcg
1261 gtggaagtta cgctagcatt ctgtaaacgg gcagcaatcg cccagcagtt agtagggtcc
1321 cctctacctc tcagggagat gtaacaacgc caccttatgg gactatcaag ctgacgctgg
1381 cttctgtgca gacaaactgc gcccacgagt tcttccctga cgccgctctc gcgcaggcaa
1441 gggaactcga tgaatactac gcaaagcaca agagacccgt tggtccactc catggcctcc
1501 ccatctctct caaagaccag cttcgagtca aggtacaccg ttgcccctaa gtcgttagat
1561 gtcccttttt gtcagctaac atatgccacc agggctacga aacatcaatg ggctacatct
1621 catggctaaa caagtacgac gaaggggact cggttctgac aaccatgctc cgcaaagccg
1681 gtgccgtctt ctacgtcaag acctctgtcc cgcagaccct gatggtctgc gagacagtca
1741 acaacatcat cgggcgcacc gtcaacccac gcaacaagaa ctggtcgtgc ggcggcagtt
1801 ctggtggtga gggtgcgatc gttgggattc gtggtggcgt catcggtgta ggaacggata
1861 tcggtggctc gattcgagtg ccggccgcgt tcaacttcct gtacggtcta aggccgagtc
1921 atgggcggct gccgtatgca aagatggcga acagcatgga gggtcaggag acggtgcaca
1981 gcgttgtcgg gccgattacg cactctgttg agggtgagtc cttcgcctct tccttctttt
2041 cctgctctat accaggcctc cactgtcctc ctttcttgct ttttatacta tatacgagac
2101 cggcagtcac tgatgaagta tgttagacct ccgcctcttc accaaatccg tcctcggtca
2161 ggagccatgg aaatacgact ccaaggtcat ccccatgccc tggcgccagt ccgagtcgga
2221 cattattgcc tccaagatca agaacggcgg gctcaatatc ggctactaca acttcgacgg
2281 caatgtcctt ccacaccctc ctatcctgcg cggcgtggaa accaccgtcg ccgcactcgc
2341 caaagccggt cacaccgtga ccccgtggac gccatacaag cacgatttcg gccacgatct
2401 catctcccat atctacgcgg ctgacggcag cgccgacgta atgcgcgata tcagtgcatc
2461 cggcgagccg gcgattccaa atatcaaaga cctactgaac ccgaacatca aagctgttaa
2521 catgaacgag ctctgggaca cgcatctcca gaagtggaat taccagatgg agtaccttga
2581 gaaatggcgg gaggctgaag aaaaggccgg gaaggaactg gacgccatca tcgcgccgat
2641 tacgcctacc gctgcggtac ggcatgacca gttccggtac tatgggtatg cctctgtgat
2701 caacctgctg gatttcacga gcgtggttgt tccggttacc tttgcggata agaacatcga
2761 taagaagaat gagagtttca aggcggttag tgagcttgat gccctcgtgc aggaagagta
2821 tgatccggag gcgtaccatg gggcaccggt tgcagtgcag gttatcggac ggagactcag
2881 tgaagagagg acgttggcga ttgcagagga agtggggaag ttgctgggaa atgtggtgac
2941 tccatagcta ataagtgtca gatagcaatt tgcacaagaa atcaatacca gcaactgtaa
3001 ataagcgctg aagtgaccat gccatgctac gaaagagcag aaaaaaacct gccgtagaac
3061 cgaagagata tgacacgctt ccatctctca aaggaagaat cccttcaggg ttgcgtttcc
3121 agtctagaca cgtataacgg cacaagtgtc tctcaccaaa tgggttatat ctcaaatgtg
3181 atctaaggat ggaaagccca gaatattggc tgggttgatg gctgcttcga gtgcagtctc
3241 atgctgccac aggtgactct ggatggcccc ataccactca acccatggta cccgtgcctc
3301 aggggtgagc tggttgttgc cttgcggtag agtaataacg atagctcagc cttgcaggtg
3361 atttccgcgt ctgtctattg tccttattac tgtgtcgagt ccccaagttt tcttccaata
3421 gacatcagta
//