LOCUS LT743389 3417 bp DNA circular SYN 15-FEB-2017
DEFINITION Human ORFeome Gateway entry vector pENTR223-TRIM61, complete
sequence.
ACCESSION LT743389
VERSION LT743389.1
KEYWORDS .
SOURCE Human ORFeome Gateway entry vector
ORGANISM Human ORFeome Gateway entry vector
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 3417)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (06-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
REFERENCE 3
AUTHORS Rual J.F., Hirozane-Kishikawa T., Hao T., Bertin N., Li S.,
Dricot A., Li N., Rosenberg J., Lamesch P., Vidalain P.O.,
Clingingsmith T.R., Hartley J.L., Esposito D., Cheo D., Moore T.,
Simmons B., Sequerra R., Bosak S., Doucette-Stamm L., Le Peuch C.,
Vandenhaute J., Cusick M.E., Albala J.S., Hill D.E., Vidal M.
TITLE Human ORFeome version 1.1: a platform for reverse proteomics
JOURNAL Genome Res. 14(10B), 2128-2135(2004).
PUBMED 15489335
REMARK This publication describes the construction of the original ORFeome
v1.1 library
REFERENCE 4
AUTHORS Yang X., Boehm J.S., Yang X., Salehi-Ashtiani K., Hao T., Shen Y.,
Lubonja R., Thomas S.R., Alkan O., Bhimdi T., Green T.M.,
Johannessen C.M., Silver S.J., Nguyen C., Murray R.R.,
Hieronymus H., Balcha D., Fan C., Lin C., Ghamsari L., Vidal M.,
Hahn W.C., Hill D.E., Root D.E.
TITLE A public genome-scale lentiviral expression library of human ORFs
JOURNAL Nat. Methods 8(8), 659-661(2011).
PUBMED 21706014
REMARK This publication describes the construction of the ORFeome v8.1
library
FEATURES Location/Qualifiers
source 1..3417
/organism="Human ORFeome Gateway entry vector"
/mol_type="other DNA"
/note="vector pENTR223-TRIM61"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP ORF81116-E05."
/db_xref="taxon:1958742"
regulatory complement(88..115)
/note="T2 terminator"
/regulatory_class="terminator"
regulatory 247..290
/note="T1 terminator"
/regulatory_class="terminator"
regulatory 390..487
/note="Gateway recombination site attL1"
/regulatory_class="attenuator"
CDS 488..>1116
/transl_table=11
/note="hTRIM61"
/note="incomplete termination codon for C-terminal fusion"
/protein_id="SJX45240.1"
/translation="MEFVTALADLRAEASCPICLDYLKDPVTISCGHNFCLSCIIMSW
KDLHDSFPCPFCHFCCPERKFISNPQLGSLTEIAKQLQIRSKKRKRQEEKHVCKKHNQ
VLTFFCQKDLELLCPRCSLSTDHQHHCVWPIKKAASYHRKKLEEYNAPWKERVELIEK
VITMQTRKSLELKKKMESPSVTRLECSCTISAHFNLRLPGSSDSSASGS"
regulatory complement(1117..1214)
/note="Gateway recombination site attL2"
/regulatory_class="attenuator"
regulatory complement(1235..1251)
/note="T7 promoter"
/regulatory_class="promoter"
CDS 1484..2494
/transl_table=11
/note="streptomycin/spectinomycin resistance (aadA)"
/protein_id="SJX45241.1"
/translation="MRSRNWSRTLTERSGGNGAVAVFMACYDCFFGVQSMPRASKQQA
RYAVGRCLMLWSSNDVTQQGSRPKTKLNIMREAVIAEVSTQLSEVVGVIERHLEPTLL
AVHLYGSAVDGGLKPHSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVE
VTIVVHDDIIPWRYPAKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVAL
VGPAAEELFDPVPEQDLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIA
PKDVAADWAMERLPAQYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVV
GK"
rep_origin 2957..3359
/note="pMB1 ori"
BASE COUNT 854 a 867 c 880 g 816 t
ORIGIN
1 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
61 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
121 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
181 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
241 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
301 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
361 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
421 ctgttcgttg caacaaattg ataagcaatg cttttttata atgccaactt tgtacaaaaa
481 agttggcatg gaatttgtta cggccctggc tgacctccga gcagaggcta gctgtcccat
541 ctgtctggac tacttgaaag acccagtgac catcagctgt gggcataact tctgtctctc
601 ctgcatcatt atgtcctgga aggatctaca tgatagtttc ccctgcccct tttgccactt
661 ttgctgtcca gaaaggaaat ttataagcaa tccccagctg ggtagtttga ctgaaattgc
721 taagcaactc cagataagaa gcaagaagag gaagaggcag gaagagaagc atgtgtgtaa
781 gaagcataat caggttttga ctttcttctg tcagaaagac ctagagcttt tatgtccaag
841 gtgcagtttg tccactgatc accagcatca ctgtgtttgg cccataaaga aggctgcctc
901 ctatcatagg aaaaaactgg aggaatacaa tgcaccgtgg aaggagagag tggaactaat
961 tgaaaaagtc ataactatgc aaaccaggaa atcactggaa ctgaagaaaa agatggagtc
1021 tccttctgtc accaggctgg agtgcagttg cacgatctcg gctcacttca acctccgcct
1081 cccgggatca agcgattctt ctgcctctgg ctcctgccca actttcttgt acaaagttgg
1141 cattataaga aagcattgct tatcaatttg ttgcaacgaa caggtcacta tcagtcaaaa
1201 taaaatcatt atttgccatc cagctgatat cccctatagt gagtcgtatt acatggtcat
1261 agctgtttcc tggcagctct ggcccgtgtc tcaaaatctc tgatgttaca ttgcacaaga
1321 taaaaatata tcatcatgcc tcctctagac cagccaggac agaaatgcct cgacttcgct
1381 gctgcccaag gttgccgggt gacgcacacc gtggaaacgg atgaaggcac gaacccagtg
1441 gacataagcc tgttcggttc gtaagctgta atgcaagtag cgtatgcgct cacgcaactg
1501 gtccagaacc ttgaccgaac gcagcggtgg taacggcgca gtggcggttt tcatggcttg
1561 ttatgactgt ttttttgggg tacagtctat gcctcgggca tccaagcagc aagcgcgtta
1621 cgccgtgggt cgatgtttga tgttatggag cagcaacgat gttacgcagc agggcagtcg
1681 ccctaaaaca aagttaaaca tcatgaggga agcggtgatc gccgaagtat cgactcaact
1741 atcagaggta gttggcgtca tcgagcgcca tctcgaaccg acgttgctgg ccgtacattt
1801 gtacggctcc gcagtggatg gcggcctgaa gccacacagt gatattgatt tgctggttac
1861 ggtgaccgta aggcttgatg aaacaacgcg gcgagctttg atcaacgacc ttttggaaac
1921 ttcggcttcc cctggagaga gcgagattct ccgcgctgta gaagtcacca ttgttgtgca
1981 cgacgacatc attccgtggc gttatccagc taagcgcgaa ctgcaatttg gagaatggca
2041 gcgcaatgac attcttgcag gtatcttcga gccagccacg atcgacattg atctggctat
2101 cttgctgaca aaagcaagag aacatagcgt tgccttggta ggtccagcgg cggaggaact
2161 ctttgatccg gttcctgaac aggatctatt tgaggcgcta aatgaaacct taacgctatg
2221 gaactcgccg cccgactggg ctggcgatga gcgaaatgta gtgcttacgt tgtcccgcat
2281 ttggtacagc gcagtaaccg gcaaaatcgc gccgaaggat gtcgctgccg actgggcaat
2341 ggagcgcctg ccggcccagt atcagcccgt catacttgaa gctagacagg cttatcttgg
2401 acaagaagaa gatcgcttgg cctcgcgcgc agatcagttg gaagaatttg tccactacgt
2461 gaaaggcgag atcaccaagg tagtcggcaa ataaccctcg agccacccat gaccaaaatc
2521 ccttaacgtg agttacgcgt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
2581 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
2641 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
2701 tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca
2761 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
2821 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
2881 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
2941 aacgacctac accgaactga gatacctaca gcgtgagcat tgagaaagcg ccacgcttcc
3001 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
3061 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
3121 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
3181 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
3241 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
3301 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
3361 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggca
//