LOCUS LT739733 4011 bp DNA circular SYN 15-FEB-2017
DEFINITION Human ORFeome Gateway entry vector pENTR223-TRIM13, complete
sequence.
ACCESSION LT739733
VERSION LT739733.1
KEYWORDS .
SOURCE Human ORFeome Gateway entry vector
ORGANISM Human ORFeome Gateway entry vector
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 4011)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (06-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
REFERENCE 3
AUTHORS Rual J.F., Hirozane-Kishikawa T., Hao T., Bertin N., Li S.,
Dricot A., Li N., Rosenberg J., Lamesch P., Vidalain P.O.,
Clingingsmith T.R., Hartley J.L., Esposito D., Cheo D., Moore T.,
Simmons B., Sequerra R., Bosak S., Doucette-Stamm L., Le Peuch C.,
Vandenhaute J., Cusick M.E., Albala J.S., Hill D.E., Vidal M.
TITLE Human ORFeome version 1.1: a platform for reverse proteomics
JOURNAL Genome Res. 14(10B), 2128-2135(2004).
PUBMED 15489335
REMARK This publication describes the construction of the original ORFeome
v1.1 library
REFERENCE 4
AUTHORS Yang X., Boehm J.S., Yang X., Salehi-Ashtiani K., Hao T., Shen Y.,
Lubonja R., Thomas S.R., Alkan O., Bhimdi T., Green T.M.,
Johannessen C.M., Silver S.J., Nguyen C., Murray R.R.,
Hieronymus H., Balcha D., Fan C., Lin C., Ghamsari L., Vidal M.,
Hahn W.C., Hill D.E., Root D.E.
TITLE A public genome-scale lentiviral expression library of human ORFs
JOURNAL Nat. Methods 8(8), 659-661(2011).
PUBMED 21706014
REMARK This publication describes the construction of the ORFeome v8.1
library
FEATURES Location/Qualifiers
source 1..4011
/organism="Human ORFeome Gateway entry vector"
/mol_type="other DNA"
/note="vector pENTR223-TRIM13"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP ORF81076-F08."
/db_xref="taxon:1958742"
regulatory complement(88..115)
/note="T2 terminator"
/regulatory_class="terminator"
regulatory 247..290
/note="T1 terminator"
/regulatory_class="terminator"
regulatory 390..487
/note="Gateway recombination site attL1"
/regulatory_class="attenuator"
CDS 488..>1710
/transl_table=11
/note="hTRIM13"
/note="incomplete termination codon for C-terminal fusion"
/protein_id="SJX37928.1"
/translation="MELLEEDLTCPICCSLFDDPRVLPCSHNFCKKCLEGILEGSVRN
SLWRPAPFKCPTCRKETSATGINSLQVNYSLKGIVEKYNKIKISPKMPVCKGHLGQPL
NIFCLTDMQLICGICATRGEHTKHVFCSIEDAYAQERDAFESLFQSFETWRRGDALSR
LDTLETSKRKSLQLLTKDSDKVKEFFEKLQHTLDQKKNEILSDFETMKLAVMQAYDPE
INKLNTILQEQRMAFNIAEAFKDVSEPIVFLQQMQEFREKIKVIKETPLPPSNLPASP
LMKNFDTSQWEDIKLVDVDKLSLPQDTGTFISKIPWSFYKLFLLILLLGLVIVFGPTM
FLEWSLFDDLATWKGCLSNFSSYLTKTADFIEQSVFYWEQVTDGFFIFNERFKNFTLV
VLNNVAEFVCKYKLL"
regulatory complement(1711..1808)
/note="Gateway recombination site attL2"
/regulatory_class="attenuator"
regulatory complement(1829..1845)
/note="T7 promoter"
/regulatory_class="promoter"
CDS 2078..3088
/transl_table=11
/note="streptomycin/spectinomycin resistance (aadA)"
/protein_id="SJX37929.1"
/translation="MRSRNWSRTLTERSGGNGAVAVFMACYDCFFGVQSMPRASKQQA
RYAVGRCLMLWSSNDVTQQGSRPKTKLNIMREAVIAEVSTQLSEVVGVIERHLEPTLL
AVHLYGSAVDGGLKPHSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVE
VTIVVHDDIIPWRYPAKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVAL
VGPAAEELFDPVPEQDLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIA
PKDVAADWAMERLPAQYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVV
GK"
rep_origin 3551..3953
/note="pMB1 ori"
BASE COUNT 1032 a 965 c 992 g 1022 t
ORIGIN
1 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
61 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
121 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
181 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
241 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
301 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
361 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
421 ctgttcgttg caacaaattg ataagcaatg cttttttata atgccaactt tgtacaaaaa
481 agttggcatg gagctgcttg aagaagatct cacatgccct atttgttgta gtctgtttga
541 tgatccacgg gttttgcctt gctcccacaa cttctgcaaa aaatgcttag aaggtatctt
601 agaagggagt gtgcggaatt ccttgtggag accagctcca ttcaagtgtc ctacatgccg
661 taaggaaact tcagctactg gaattaatag cctgcaggtt aattactccc tgaagggtat
721 tgtggaaaag tataacaaga tcaagatctc tcccaaaatg ccagtatgca aaggacactt
781 ggggcagcct ctcaacattt tctgcctgac tgatatgcag ctgatttgtg ggatctgtgc
841 tactcgtggg gagcacacca aacatgtctt ctgttctatt gaagatgcct atgctcagga
901 aagggatgcc tttgagtccc tcttccagag ctttgagacc tggcgtcggg gagatgctct
961 ttctcgcttg gataccttgg aaactagtaa gaggaaatcc ctacagttac tgactaaaga
1021 ttcagataaa gtgaaggaat tttttgagaa gttacaacac acactggatc aaaagaagaa
1081 tgaaattctg tctgactttg agaccatgaa acttgctgtt atgcaagcat atgacccaga
1141 gatcaacaaa ctcaacacca tcttgcagga gcaacggatg gcctttaaca ttgctgaggc
1201 tttcaaagat gtgtcagaac ccattgtatt tctgcaacag atgcaggagt ttagagagaa
1261 aatcaaagta atcaaggaaa ctcctttacc tccctctaat ttgcctgcaa gccctttaat
1321 gaagaacttt gataccagtc agtgggaaga cataaaacta gtcgatgtgg ataaactttc
1381 tttgcctcaa gacactggca cattcattag caagattccc tggagctttt ataagttatt
1441 tttgctaatc cttctgcttg gccttgtcat tgtctttggt cctaccatgt tcctagaatg
1501 gtcattattt gatgacctgg caacttggaa aggctgtctt tcaaacttca gttcctatct
1561 gactaaaaca gccgatttca tagaacaatc agttttttac tgggaacagg tgacagatgg
1621 gtttttcatt ttcaatgaaa gattcaagaa ttttactttg gtggtactga acaatgtggc
1681 agaatttgtg tgcaaatata aactattata cccaactttc ttgtacaaag ttggcattat
1741 aagaaagcat tgcttatcaa tttgttgcaa cgaacaggtc actatcagtc aaaataaaat
1801 cattatttgc catccagctg atatccccta tagtgagtcg tattacatgg tcatagctgt
1861 ttcctggcag ctctggcccg tgtctcaaaa tctctgatgt tacattgcac aagataaaaa
1921 tatatcatca tgcctcctct agaccagcca ggacagaaat gcctcgactt cgctgctgcc
1981 caaggttgcc gggtgacgca caccgtggaa acggatgaag gcacgaaccc agtggacata
2041 agcctgttcg gttcgtaagc tgtaatgcaa gtagcgtatg cgctcacgca actggtccag
2101 aaccttgacc gaacgcagcg gtggtaacgg cgcagtggcg gttttcatgg cttgttatga
2161 ctgttttttt ggggtacagt ctatgcctcg ggcatccaag cagcaagcgc gttacgccgt
2221 gggtcgatgt ttgatgttat ggagcagcaa cgatgttacg cagcagggca gtcgccctaa
2281 aacaaagtta aacatcatga gggaagcggt gatcgccgaa gtatcgactc aactatcaga
2341 ggtagttggc gtcatcgagc gccatctcga accgacgttg ctggccgtac atttgtacgg
2401 ctccgcagtg gatggcggcc tgaagccaca cagtgatatt gatttgctgg ttacggtgac
2461 cgtaaggctt gatgaaacaa cgcggcgagc tttgatcaac gaccttttgg aaacttcggc
2521 ttcccctgga gagagcgaga ttctccgcgc tgtagaagtc accattgttg tgcacgacga
2581 catcattccg tggcgttatc cagctaagcg cgaactgcaa tttggagaat ggcagcgcaa
2641 tgacattctt gcaggtatct tcgagccagc cacgatcgac attgatctgg ctatcttgct
2701 gacaaaagca agagaacata gcgttgcctt ggtaggtcca gcggcggagg aactctttga
2761 tccggttcct gaacaggatc tatttgaggc gctaaatgaa accttaacgc tatggaactc
2821 gccgcccgac tgggctggcg atgagcgaaa tgtagtgctt acgttgtccc gcatttggta
2881 cagcgcagta accggcaaaa tcgcgccgaa ggatgtcgct gccgactggg caatggagcg
2941 cctgccggcc cagtatcagc ccgtcatact tgaagctaga caggcttatc ttggacaaga
3001 agaagatcgc ttggcctcgc gcgcagatca gttggaagaa tttgtccact acgtgaaagg
3061 cgagatcacc aaggtagtcg gcaaataacc ctcgagccac ccatgaccaa aatcccttaa
3121 cgtgagttac gcgtcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
3181 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
3241 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
3301 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
3361 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
3421 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
3481 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
3541 ctacaccgaa ctgagatacc tacagcgtga gcattgagaa agcgccacgc ttcccgaagg
3601 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
3661 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
3721 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
3781 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
3841 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
3901 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
3961 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc a
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