LOCUS LT735404 3414 bp DNA circular SYN 15-FEB-2017
DEFINITION Human ORFeome Gateway entry vector pENTR223-TRIM48, complete
sequence.
ACCESSION LT735404
VERSION LT735404.1
KEYWORDS .
SOURCE Human ORFeome Gateway entry vector
ORGANISM Human ORFeome Gateway entry vector
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 3414)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (06-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
REFERENCE 3
AUTHORS Rual J.F., Hirozane-Kishikawa T., Hao T., Bertin N., Li S.,
Dricot A., Li N., Rosenberg J., Lamesch P., Vidalain P.O.,
Clingingsmith T.R., Hartley J.L., Esposito D., Cheo D., Moore T.,
Simmons B., Sequerra R., Bosak S., Doucette-Stamm L., Le Peuch C.,
Vandenhaute J., Cusick M.E., Albala J.S., Hill D.E., Vidal M.
TITLE Human ORFeome version 1.1: a platform for reverse proteomics
JOURNAL Genome Res. 14(10B), 2128-2135(2004).
PUBMED 15489335
REMARK This publication describes the construction of the original ORFeome
v1.1 library
REFERENCE 4
AUTHORS Yang X., Boehm J.S., Yang X., Salehi-Ashtiani K., Hao T., Shen Y.,
Lubonja R., Thomas S.R., Alkan O., Bhimdi T., Green T.M.,
Johannessen C.M., Silver S.J., Nguyen C., Murray R.R.,
Hieronymus H., Balcha D., Fan C., Lin C., Ghamsari L., Vidal M.,
Hahn W.C., Hill D.E., Root D.E.
TITLE A public genome-scale lentiviral expression library of human ORFs
JOURNAL Nat. Methods 8(8), 659-661(2011).
PUBMED 21706014
REMARK This publication describes the construction of the ORFeome v8.1
library
FEATURES Location/Qualifiers
source 1..3414
/organism="Human ORFeome Gateway entry vector"
/mol_type="other DNA"
/note="vector pENTR223-TRIM48"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP ORF81030-A10."
/db_xref="taxon:1958742"
regulatory complement(88..115)
/note="T2 terminator"
/regulatory_class="terminator"
regulatory 247..290
/note="T1 terminator"
/regulatory_class="terminator"
regulatory 390..487
/note="Gateway recombination site attL1"
/regulatory_class="attenuator"
CDS 488..>1113
/transl_table=11
/note="hTRIM48"
/note="incomplete termination codon for C-terminal fusion"
/protein_id="SJX29270.1"
/translation="MNSGISQVFQRELTCPICMNYFIDPVTIDCGHSFCRPCFYLNWQ
DIPILTQCFECIKTIQQRNLKTNIRLKKMASLARKASLWLFLSSEEQMCGIHRETKKM
FCEVDRSLLCLLCSSSQEHRYHRHCPAEWAAEEHWEKLLKKMQSLWEKACENQRNLNV
ETTRISHWKAFGDILYRSESVLLHMPQPLNLALRAGPITGLRDRLNQF"
regulatory complement(1114..1211)
/note="Gateway recombination site attL2"
/regulatory_class="attenuator"
regulatory complement(1232..1248)
/note="T7 promoter"
/regulatory_class="promoter"
CDS 1481..2491
/transl_table=11
/note="streptomycin/spectinomycin resistance (aadA)"
/protein_id="SJX29271.1"
/translation="MRSRNWSRTLTERSGGNGAVAVFMACYDCFFGVQSMPRASKQQA
RYAVGRCLMLWSSNDVTQQGSRPKTKLNIMREAVIAEVSTQLSEVVGVIERHLEPTLL
AVHLYGSAVDGGLKPHSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVE
VTIVVHDDIIPWRYPAKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVAL
VGPAAEELFDPVPEQDLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIA
PKDVAADWAMERLPAQYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVV
GK"
rep_origin 2954..3356
/note="pMB1 ori"
BASE COUNT 855 a 873 c 886 g 800 t
ORIGIN
1 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
61 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
121 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
181 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
241 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
301 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
361 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
421 ctgttcgttg caacaaattg ataagcaatg cttttttata atgccaactt tgtacaaaaa
481 agttggcatg aattctggaa tctcgcaagt cttccagagg gaactcacct gccccatctg
541 catgaactac ttcatagacc cggtcaccat agactgtggg cacagctttt gcaggccctg
601 tttctacctc aactggcaag acatcccaat tcttactcag tgctttgaat gcataaagac
661 aatacagcag agaaacctca aaactaacat tcgattgaag aagatggctt cccttgccag
721 aaaagccagt ctctggctat tcctgagctc tgaggagcaa atgtgtggca ttcacaggga
781 gacaaagaag atgttctgtg aagtggacag gagcctgctc tgtttgctgt gctccagctc
841 tcaggagcac cggtatcaca gacactgtcc cgctgagtgg gctgctgagg aacactggga
901 gaagctgtta aagaaaatgc agtctttatg ggaaaaagct tgtgaaaatc agagaaacct
961 gaatgtggaa accaccagaa tcagccactg gaaggctttt ggagacatat tatacaggag
1021 tgagtccgtg ctgctgcaca tgccccagcc tctgaatcta gcgctcaggg cagggcccat
1081 cactggactg agggacaggc tcaaccaatt ctgcccaact ttcttgtaca aagttggcat
1141 tataagaaag cattgcttat caatttgttg caacgaacag gtcactatca gtcaaaataa
1201 aatcattatt tgccatccag ctgatatccc ctatagtgag tcgtattaca tggtcatagc
1261 tgtttcctgg cagctctggc ccgtgtctca aaatctctga tgttacattg cacaagataa
1321 aaatatatca tcatgcctcc tctagaccag ccaggacaga aatgcctcga cttcgctgct
1381 gcccaaggtt gccgggtgac gcacaccgtg gaaacggatg aaggcacgaa cccagtggac
1441 ataagcctgt tcggttcgta agctgtaatg caagtagcgt atgcgctcac gcaactggtc
1501 cagaaccttg accgaacgca gcggtggtaa cggcgcagtg gcggttttca tggcttgtta
1561 tgactgtttt tttggggtac agtctatgcc tcgggcatcc aagcagcaag cgcgttacgc
1621 cgtgggtcga tgtttgatgt tatggagcag caacgatgtt acgcagcagg gcagtcgccc
1681 taaaacaaag ttaaacatca tgagggaagc ggtgatcgcc gaagtatcga ctcaactatc
1741 agaggtagtt ggcgtcatcg agcgccatct cgaaccgacg ttgctggccg tacatttgta
1801 cggctccgca gtggatggcg gcctgaagcc acacagtgat attgatttgc tggttacggt
1861 gaccgtaagg cttgatgaaa caacgcggcg agctttgatc aacgaccttt tggaaacttc
1921 ggcttcccct ggagagagcg agattctccg cgctgtagaa gtcaccattg ttgtgcacga
1981 cgacatcatt ccgtggcgtt atccagctaa gcgcgaactg caatttggag aatggcagcg
2041 caatgacatt cttgcaggta tcttcgagcc agccacgatc gacattgatc tggctatctt
2101 gctgacaaaa gcaagagaac atagcgttgc cttggtaggt ccagcggcgg aggaactctt
2161 tgatccggtt cctgaacagg atctatttga ggcgctaaat gaaaccttaa cgctatggaa
2221 ctcgccgccc gactgggctg gcgatgagcg aaatgtagtg cttacgttgt cccgcatttg
2281 gtacagcgca gtaaccggca aaatcgcgcc gaaggatgtc gctgccgact gggcaatgga
2341 gcgcctgccg gcccagtatc agcccgtcat acttgaagct agacaggctt atcttggaca
2401 agaagaagat cgcttggcct cgcgcgcaga tcagttggaa gaatttgtcc actacgtgaa
2461 aggcgagatc accaaggtag tcggcaaata accctcgagc cacccatgac caaaatccct
2521 taacgtgagt tacgcgtcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
2581 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
2641 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
2701 cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca
2761 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
2821 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
2881 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
2941 gacctacacc gaactgagat acctacagcg tgagcattga gaaagcgcca cgcttcccga
3001 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
3061 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
3121 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
3181 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
3241 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
3301 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
3361 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggca
//