LOCUS LT735332 3396 bp DNA circular SYN 15-FEB-2017
DEFINITION Human ORFeome Gateway entry vector pENTR223-TRIM11, complete
sequence.
ACCESSION LT735332
VERSION LT735332.1
KEYWORDS .
SOURCE Human ORFeome Gateway entry vector
ORGANISM Human ORFeome Gateway entry vector
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 3396)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (06-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
REFERENCE 3
AUTHORS Rual J.F., Hirozane-Kishikawa T., Hao T., Bertin N., Li S.,
Dricot A., Li N., Rosenberg J., Lamesch P., Vidalain P.O.,
Clingingsmith T.R., Hartley J.L., Esposito D., Cheo D., Moore T.,
Simmons B., Sequerra R., Bosak S., Doucette-Stamm L., Le Peuch C.,
Vandenhaute J., Cusick M.E., Albala J.S., Hill D.E., Vidal M.
TITLE Human ORFeome version 1.1: a platform for reverse proteomics
JOURNAL Genome Res. 14(10B), 2128-2135(2004).
PUBMED 15489335
REMARK This publication describes the construction of the original ORFeome
v1.1 library
REFERENCE 4
AUTHORS Yang X., Boehm J.S., Yang X., Salehi-Ashtiani K., Hao T., Shen Y.,
Lubonja R., Thomas S.R., Alkan O., Bhimdi T., Green T.M.,
Johannessen C.M., Silver S.J., Nguyen C., Murray R.R.,
Hieronymus H., Balcha D., Fan C., Lin C., Ghamsari L., Vidal M.,
Hahn W.C., Hill D.E., Root D.E.
TITLE A public genome-scale lentiviral expression library of human ORFs
JOURNAL Nat. Methods 8(8), 659-661(2011).
PUBMED 21706014
REMARK This publication describes the construction of the ORFeome v8.1
library
FEATURES Location/Qualifiers
source 1..3396
/organism="Human ORFeome Gateway entry vector"
/mol_type="other DNA"
/note="vector pENTR223-TRIM11"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP ORF81029-C05."
/db_xref="taxon:1958742"
regulatory complement(88..115)
/note="T2 terminator"
/regulatory_class="terminator"
regulatory 247..290
/note="T1 terminator"
/regulatory_class="terminator"
regulatory 390..487
/note="Gateway recombination site attL1"
/regulatory_class="attenuator"
CDS 488..>1095
/transl_table=11
/note="hTRIM11"
/note="incomplete termination codon for C-terminal fusion"
/protein_id="SJX29126.1"
/translation="MELRTVCRVPGLVETLRRFRGDVTLDPDTANPELILSEDRRSVQ
RGDLRQALPDSPERFDPGPCVLGQERFTSGRHYWEVEVGDRTSWALGVCRENVNRKEK
GELSAGNGFWILVFLGSYYNSSERALAPLRDPPRRVGIFLDYEAGHLSFYSATDGSLL
FIFPEIPFSGTLRPLFSPLSSSPTPMTICRPKGGSGDTLAPQ"
regulatory complement(1096..1193)
/note="Gateway recombination site attL2"
/regulatory_class="attenuator"
regulatory complement(1214..1230)
/note="T7 promoter"
/regulatory_class="promoter"
CDS 1463..2473
/transl_table=11
/note="streptomycin/spectinomycin resistance (aadA)"
/protein_id="SJX29127.1"
/translation="MRSRNWSRTLTERSGGNGAVAVFMACYDCFFGVQSMPRASKQQA
RYAVGRCLMLWSSNDVTQQGSRPKTKLNIMREAVIAEVSTQLSEVVGVIERHLEPTLL
AVHLYGSAVDGGLKPHSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVE
VTIVVHDDIIPWRYPAKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVAL
VGPAAEELFDPVPEQDLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIA
PKDVAADWAMERLPAQYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVV
GK"
rep_origin 2936..3338
/note="pMB1 ori"
BASE COUNT 781 a 911 c 936 g 768 t
ORIGIN
1 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
61 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
121 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
181 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
241 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
301 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
361 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
421 ctgttcgttg caacaaattg ataagcaatg cttttttata atgccaactt tgtacaaaaa
481 agttggcatg gagctgagga ccgtgtgcag ggtcccggga ctggtagaga cactgcggag
541 gtttcgaggg gacgtgacct tggacccgga caccgccaac cctgagctga tcctgtctga
601 agacaggcgg agcgtgcagc ggggggacct acggcaggcc ctgccggaca gcccagagcg
661 ctttgacccc ggcccctgcg tgctgggcca ggagcgcttc acctcaggcc gccactactg
721 ggaggtggag gttggggacc gcaccagctg ggccctgggg gtgtgcaggg agaacgtgaa
781 caggaaggag aagggcgagc tgtccgcggg caacggcttc tggatcctgg tcttcctggg
841 gagctattac aattcctcgg aacgggcctt ggctccactc cgggacccac ccaggcgcgt
901 ggggatcttt ctggactacg aggctggaca tctctctttc tacagtgcca ccgatgggtc
961 actgctattc atctttcccg agatcccctt ctcggggacg ctgcggcccc tcttctcacc
1021 cctgtccagc agcccgaccc cgatgactat ctgccggccg aaaggtgggt ccggggacac
1081 cctggctccc cagtgcccaa ctttcttgta caaagttggc attataagaa agcattgctt
1141 atcaatttgt tgcaacgaac aggtcactat cagtcaaaat aaaatcatta tttgccatcc
1201 agctgatatc ccctatagtg agtcgtatta catggtcata gctgtttcct ggcagctctg
1261 gcccgtgtct caaaatctct gatgttacat tgcacaagat aaaaatatat catcatgcct
1321 cctctagacc agccaggaca gaaatgcctc gacttcgctg ctgcccaagg ttgccgggtg
1381 acgcacaccg tggaaacgga tgaaggcacg aacccagtgg acataagcct gttcggttcg
1441 taagctgtaa tgcaagtagc gtatgcgctc acgcaactgg tccagaacct tgaccgaacg
1501 cagcggtggt aacggcgcag tggcggtttt catggcttgt tatgactgtt tttttggggt
1561 acagtctatg cctcgggcat ccaagcagca agcgcgttac gccgtgggtc gatgtttgat
1621 gttatggagc agcaacgatg ttacgcagca gggcagtcgc cctaaaacaa agttaaacat
1681 catgagggaa gcggtgatcg ccgaagtatc gactcaacta tcagaggtag ttggcgtcat
1741 cgagcgccat ctcgaaccga cgttgctggc cgtacatttg tacggctccg cagtggatgg
1801 cggcctgaag ccacacagtg atattgattt gctggttacg gtgaccgtaa ggcttgatga
1861 aacaacgcgg cgagctttga tcaacgacct tttggaaact tcggcttccc ctggagagag
1921 cgagattctc cgcgctgtag aagtcaccat tgttgtgcac gacgacatca ttccgtggcg
1981 ttatccagct aagcgcgaac tgcaatttgg agaatggcag cgcaatgaca ttcttgcagg
2041 tatcttcgag ccagccacga tcgacattga tctggctatc ttgctgacaa aagcaagaga
2101 acatagcgtt gccttggtag gtccagcggc ggaggaactc tttgatccgg ttcctgaaca
2161 ggatctattt gaggcgctaa atgaaacctt aacgctatgg aactcgccgc ccgactgggc
2221 tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt tggtacagcg cagtaaccgg
2281 caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg gagcgcctgc cggcccagta
2341 tcagcccgtc atacttgaag ctagacaggc ttatcttgga caagaagaag atcgcttggc
2401 ctcgcgcgca gatcagttgg aagaatttgt ccactacgtg aaaggcgaga tcaccaaggt
2461 agtcggcaaa taaccctcga gccacccatg accaaaatcc cttaacgtga gttacgcgtc
2521 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
2581 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
2641 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
2701 accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
2761 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
2821 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
2881 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
2941 atacctacag cgtgagcatt gagaaagcgc cacgcttccc gaagggagaa aggcggacag
3001 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
3061 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
3121 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
3181 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
3241 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
3301 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct
3361 ccccgcgcgt tggccgattc attaatgcag ctggca
//