LOCUS LT734085 5022 bp DNA circular SYN 15-FEB-2017
DEFINITION Human ORFeome Gateway entry vector pENTR223-TRIM2, complete
sequence.
ACCESSION LT734085
VERSION LT734085.1
KEYWORDS .
SOURCE Human ORFeome Gateway entry vector
ORGANISM Human ORFeome Gateway entry vector
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 5022)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (06-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
REFERENCE 3
AUTHORS Rual J.F., Hirozane-Kishikawa T., Hao T., Bertin N., Li S.,
Dricot A., Li N., Rosenberg J., Lamesch P., Vidalain P.O.,
Clingingsmith T.R., Hartley J.L., Esposito D., Cheo D., Moore T.,
Simmons B., Sequerra R., Bosak S., Doucette-Stamm L., Le Peuch C.,
Vandenhaute J., Cusick M.E., Albala J.S., Hill D.E., Vidal M.
TITLE Human ORFeome version 1.1: a platform for reverse proteomics
JOURNAL Genome Res. 14(10B), 2128-2135(2004).
PUBMED 15489335
REMARK This publication describes the construction of the original ORFeome
v1.1 library
REFERENCE 4
AUTHORS Yang X., Boehm J.S., Yang X., Salehi-Ashtiani K., Hao T., Shen Y.,
Lubonja R., Thomas S.R., Alkan O., Bhimdi T., Green T.M.,
Johannessen C.M., Silver S.J., Nguyen C., Murray R.R.,
Hieronymus H., Balcha D., Fan C., Lin C., Ghamsari L., Vidal M.,
Hahn W.C., Hill D.E., Root D.E.
TITLE A public genome-scale lentiviral expression library of human ORFs
JOURNAL Nat. Methods 8(8), 659-661(2011).
PUBMED 21706014
REMARK This publication describes the construction of the ORFeome v8.1
library
FEATURES Location/Qualifiers
source 1..5022
/organism="Human ORFeome Gateway entry vector"
/mol_type="other DNA"
/note="vector pENTR223-TRIM2"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP ORF81015-H01."
/db_xref="taxon:1958742"
regulatory complement(88..115)
/note="T2 terminator"
/regulatory_class="terminator"
regulatory 247..290
/note="T1 terminator"
/regulatory_class="terminator"
regulatory 390..487
/note="Gateway recombination site attL1"
/regulatory_class="attenuator"
CDS 488..>2721
/transl_table=11
/note="hTRIM2"
/note="incomplete termination codon for C-terminal fusion"
/protein_id="SJX27608.1"
/translation="MASEGTNIPSPVVRQIDKQFLICSICLERYKNPKVLPCLHTFCE
RCLQNYIPAHSLTLSCPVCRQTSILPEKGVAALQNNFFITNLMDVLQRTPGSNAEESS
ILETVTAVAAGKPLSCPNHDGNVMEFYCQSCETAMCRECTEGEHAEHPTVPLKDVVEQ
HKASLQVQLDAVNKRLPEIDSALQFISEIIHQLTNQKASIVDDIHSTFDELQKTLNVR
KSVLLMELEVNYGLKHKVLQSQLDTLLQGQESIKSCSNFTAQALNHGTETEVLLVKKQ
MSEKLNELADQDFPLHPRENDQLDFIVETEGLKKSIHNLGTILTTNAVASETVATGEG
LRQTIIGQPMSVTITTKDKDGELCKTGNAYLTAELSTPDGSVADGEILDNKNGTYEFL
YTVQKEGDFTLSLRLYDQHIRGSPFKLKVIRSADVSPTTEGVKRRVKSPGSGHVKQKA
VKRPASMYSTGKRKENPIEDDLIFRVGTKGRNKGEFTNLQGVAASTNGKILIADSNNQ
CVQIFSNDGQFKSRFGIRGRSPGQLQRPTGVAVHPSGDIIIADYDNKWVSIFSSDGKF
KTKIGSGKLMGPKGVSVDRNGHIIVVDNKACCVFIFQPNGKIVTRFGSRGNGDRQFAG
PHFAAVNSNNEIIITDFHNHSVKVFNQEGEFMLKFGSNGEGNGQFNAPTGVAVDSNGN
IIVADWGNSRIQVFDGSGSFLSYINTSADPLYGPQGLALTSDGHVVVADSGNHCFKVY
RYLQ"
regulatory complement(2722..2819)
/note="Gateway recombination site attL2"
/regulatory_class="attenuator"
regulatory complement(2840..2856)
/note="T7 promoter"
/regulatory_class="promoter"
CDS 3089..4099
/transl_table=11
/note="streptomycin/spectinomycin resistance (aadA)"
/protein_id="SJX27609.1"
/translation="MRSRNWSRTLTERSGGNGAVAVFMACYDCFFGVQSMPRASKQQA
RYAVGRCLMLWSSNDVTQQGSRPKTKLNIMREAVIAEVSTQLSEVVGVIERHLEPTLL
AVHLYGSAVDGGLKPHSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVE
VTIVVHDDIIPWRYPAKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVAL
VGPAAEELFDPVPEQDLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIA
PKDVAADWAMERLPAQYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVV
GK"
rep_origin 4562..4964
/note="pMB1 ori"
BASE COUNT 1287 a 1278 c 1320 g 1137 t
ORIGIN
1 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
61 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
121 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
181 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
241 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
301 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
361 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
421 ctgttcgttg caacaaattg ataagcaatg cttttttata atgccaactt tgtacaaaaa
481 agttggcatg gccagtgaag gcaccaacat cccaagtcct gtggtgcgcc agattgacaa
541 gcagtttctg atttgcagta tatgcctgga acggtacaag aatcccaagg ttctcccctg
601 tctgcacact ttctgcgaga ggtgcctgca gaactacatt cctgcccaca gtttaaccct
661 ctcctgccca gtgtgccgcc agacctccat cctgcccgag aaaggggtgg ccgcgctcca
721 gaacaatttc ttcatcacaa acctgatgga cgtgctgcag cgaactccag gcagcaacgc
781 tgaggagtct tccatcctgg agacagtcac tgctgtggct gcgggaaagc ctctctcttg
841 cccaaaccac gatgggaatg tgatggaatt ttactgccag tcctgtgaga ctgccatgtg
901 tcgggagtgc acggaggggg agcacgcaga gcaccccaca gttccactca aggatgtggt
961 ggaacagcac aaggcctcgc tccaggtcca gctggatgct gtcaacaaaa ggctcccaga
1021 aatagattct gctcttcagt tcatctctga aatcattcat cagttaacca accaaaaggc
1081 cagcatcgtg gatgacattc attccacctt tgatgagctc cagaagactt taaatgtgcg
1141 caagagtgtg ctgcttatgg aattggaggt caactatggc ctcaaacaca aagtcctcca
1201 gtcgcagctg gatactctgc tccaggggca ggagagcatt aagagctgca gcaacttcac
1261 agcgcaggcc ctcaaccatg gcacggagac cgaggtccta ctggtgaaga agcagatgag
1321 cgagaagctg aacgagctgg ccgaccagga cttccccttg cacccgcggg agaacgacca
1381 gctggatttc atcgtggaaa ccgaggggct gaagaagtcc atccacaacc tcgggacgat
1441 cttaaccacc aacgccgttg cctcagagac agtggccacg ggcgaggggc tgcggcagac
1501 catcatcggg cagcccatgt ccgtcaccat caccaccaag gacaaagacg gtgagctgtg
1561 caaaaccggc aacgcctacc tcaccgccga actgagcacc cccgacggga gcgtggcaga
1621 cggggagatc ctggacaaca agaacggcac ctatgagttt ttgtacactg tccagaagga
1681 aggggacttt accctgtctc tgagactcta tgaccagcac atccgaggca gcccgtttaa
1741 gctgaaagtg atccgatccg ctgatgtgtc tcccaccaca gaaggcgtga agaggcgcgt
1801 taagtccccg gggagcggcc acgtcaagca gaaagctgtg aaaagacccg caagcatgta
1861 cagcactgga aaacgaaaag agaatcccat cgaagacgat ttgatctttc gagtgggtac
1921 caaaggaaga aataaaggag agtttacaaa tcttcagggg gtagctgcat ctacaaatgg
1981 aaagatatta attgcagaca gtaacaacca atgtgtgcag atattttcca atgatggcca
2041 gttcaaaagt cgttttggca tacggggacg ctctccgggg cagctgcagc ggcccacagg
2101 agtggctgta catcccagtg gggacataat cattgccgat tatgataata aatgggtcag
2161 cattttctcc tccgatggga aatttaagac aaaaattgga tcaggaaagc tgatgggacc
2221 caaaggagtt tctgtggacc gcaatgggca cattattgtt gtggacaaca aggcgtgctg
2281 cgtgtttatc ttccagccaa acgggaaaat agtcaccagg tttggtagcc gaggaaatgg
2341 ggacaggcag tttgcaggtc cccattttgc agctgtaaat agcaataatg agattattat
2401 tacagatttc cataatcatt ctgtcaaggt gtttaatcag gaaggagaat tcatgttgaa
2461 gtttggctca aatggagaag gaaatgggca gtttaatgct ccaacaggtg tagcagtgga
2521 ttcaaatgga aacatcattg tggccgactg gggaaacagc aggatccagg tttttgatgg
2581 gagtggatca tttttgtcct acattaacac atctgctgac ccactctatg gcccccaagg
2641 cctggcccta acttcagatg gtcatgttgt ggttgcagac tctggaaatc actgtttcaa
2701 agtctatcga tacttacagt acccaacttt cttgtacaaa gttggcatta taagaaagca
2761 ttgcttatca atttgttgca acgaacaggt cactatcagt caaaataaaa tcattatttg
2821 ccatccagct gatatcccct atagtgagtc gtattacatg gtcatagctg tttcctggca
2881 gctctggccc gtgtctcaaa atctctgatg ttacattgca caagataaaa atatatcatc
2941 atgcctcctc tagaccagcc aggacagaaa tgcctcgact tcgctgctgc ccaaggttgc
3001 cgggtgacgc acaccgtgga aacggatgaa ggcacgaacc cagtggacat aagcctgttc
3061 ggttcgtaag ctgtaatgca agtagcgtat gcgctcacgc aactggtcca gaaccttgac
3121 cgaacgcagc ggtggtaacg gcgcagtggc ggttttcatg gcttgttatg actgtttttt
3181 tggggtacag tctatgcctc gggcatccaa gcagcaagcg cgttacgccg tgggtcgatg
3241 tttgatgtta tggagcagca acgatgttac gcagcagggc agtcgcccta aaacaaagtt
3301 aaacatcatg agggaagcgg tgatcgccga agtatcgact caactatcag aggtagttgg
3361 cgtcatcgag cgccatctcg aaccgacgtt gctggccgta catttgtacg gctccgcagt
3421 ggatggcggc ctgaagccac acagtgatat tgatttgctg gttacggtga ccgtaaggct
3481 tgatgaaaca acgcggcgag ctttgatcaa cgaccttttg gaaacttcgg cttcccctgg
3541 agagagcgag attctccgcg ctgtagaagt caccattgtt gtgcacgacg acatcattcc
3601 gtggcgttat ccagctaagc gcgaactgca atttggagaa tggcagcgca atgacattct
3661 tgcaggtatc ttcgagccag ccacgatcga cattgatctg gctatcttgc tgacaaaagc
3721 aagagaacat agcgttgcct tggtaggtcc agcggcggag gaactctttg atccggttcc
3781 tgaacaggat ctatttgagg cgctaaatga aaccttaacg ctatggaact cgccgcccga
3841 ctgggctggc gatgagcgaa atgtagtgct tacgttgtcc cgcatttggt acagcgcagt
3901 aaccggcaaa atcgcgccga aggatgtcgc tgccgactgg gcaatggagc gcctgccggc
3961 ccagtatcag cccgtcatac ttgaagctag acaggcttat cttggacaag aagaagatcg
4021 cttggcctcg cgcgcagatc agttggaaga atttgtccac tacgtgaaag gcgagatcac
4081 caaggtagtc ggcaaataac cctcgagcca cccatgacca aaatccctta acgtgagtta
4141 cgcgtcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
4201 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
4261 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
4321 gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc
4381 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
4441 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
4501 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
4561 actgagatac ctacagcgtg agcattgaga aagcgccacg cttcccgaag ggagaaaggc
4621 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
4681 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
4741 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
4801 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
4861 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
4921 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc
4981 gcctctcccc gcgcgttggc cgattcatta atgcagctgg ca
//