LOCUS LT727634 9455 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian expression vector pSV529HIFNG, complete sequence.
ACCESSION LT727634
VERSION LT727634.1
KEYWORDS .
SOURCE Mammalian expression vector pSV529HIFNG
ORGANISM Mammalian expression vector pSV529HIFNG
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 9455)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..9455
/organism="Mammalian expression vector pSV529HIFNG"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 2234."
/db_xref="taxon:1945111"
regulatory 101..382
/note="SV40 early promoter"
/regulatory_class="promoter"
rep_origin 339..414
/note="SV40 ori"
misc_feature 453..698
/note="SV40 large T-antigen"
CDS 453..977
/transl_table=11
/note="SV40 small t-antigen"
/protein_id="SJL89043.1"
/translation="MDKVLNREESLQLMDLLGLERSAWGNIPLMRKAYLKKCKEFHPD
KGGDEEKMKKMNTLYKKMEDGVKYAHQPDFGGFWDATEVFASSLNPGVDAMYCKQWPE
CAKKMSANCICLLCLLRMKHENRKLYRKDPLVWVDCYCFDCFRMWFGLDLCEGTLLLW
CDIIGQTTYRDLKL"
misc_feature 699..1044
/note="intron"
misc_feature 1045..2925
/note="SV40 large T-antigen"
regulatory complement(2926..3022)
/note="polyA"
/regulatory_class="terminator"
regulatory 2926..3022
/note="polyA"
/regulatory_class="terminator"
CDS complement(3365..3865)
/transl_table=11
/note="hIFNG"
/protein_id="SJL89044.1"
/translation="MKYTSYILAFQLCIVLGSLGCYCQDPYVKEAENLKKYFNAGHSD
VADNGTLFLGILKNWKEESDRKIMQSQIVSFYFKLFKNFKDDQSIQKSVETIKEDMNV
KFFNSNKKKRDDFEKLTNYSVTDLNVQRKAIHELIQVMAELSPAAKTGKRKRSQMLFR
GRRASQ"
misc_feature complement(3962..4899)
/note="intron"
misc_feature complement(4900..5102)
/note="5UTR"
regulatory complement(5103..5398)
/note="SV40 late promoter"
/regulatory_class="promoter"
regulatory 5154..5435
/note="SV40 early promoter"
/regulatory_class="promoter"
rep_origin 5392..5467
/note="SV40 ori"
misc_feature 5506..5751
/note="SV40 large T-antigen"
CDS 5506..6030
/transl_table=11
/note="SV40 small t-antigen"
/protein_id="SJL89045.1"
/translation="MDKVLNREESLQLMDLLGLERSAWGNIPLMRKAYLKKCKEFHPD
KGGDEEKMKKMNTLYKKMEDGVKYAHQPDFGGFWDATEVFASSLNPGVDAMYCKQWPE
CAKKMSANCICLLCLLRMKHENRKLYRKDPLVWVDCYCFDCFRMWFGLDLCEGTLLLW
CDIIGQTTYRDLKL"
misc_feature 5752..6097
/note="intron"
misc_feature 6098..7160
/note="part of SV40 large t-antigen"
rep_origin complement(7444..7846)
/note="pMB1 ori"
CDS complement(8387..9247)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL89046.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
BASE COUNT 2821 a 1894 c 2087 g 2653 t
ORIGIN
1 ttctcatgtt tgacagctta tcatcggcgc agcaccatgg cctgaaataa cctctgaaag
61 aggaacttgg ttaggtacct tctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt
121 agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
181 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
241 catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
301 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
361 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
421 aggcctaggc ttttgcaaaa agctttgcaa agatggataa agttttaaac agagaggaat
481 ctttgcagct aatggacctt ctaggtcttg aaaggagtgc ctgggggaat attcctctga
541 tgagaaaggc atatttaaaa aaatgcaagg agtttcatcc tgataaagga ggagatgaag
601 aaaaaatgaa gaaaatgaat actctgtaca agaaaatgga agatggagta aaatatgctc
661 atcaacctga ctttggaggc ttctgggatg caactgaggt atttgcttct tccttaaatc
721 ctggtgttga tgcaatgtac tgcaaacaat ggcctgagtg tgcaaagaaa atgtctgcta
781 actgcatatg cttgctgtgc ttactgagga tgaagcatga aaatagaaaa ttatacagga
841 aagatccact tgtgtgggtt gattgctact gcttcgattg ctttagaatg tggtttggac
901 ttgatctttg tgaaggaacc ttacttctgt ggtgtgacat aattggacaa actacctaca
961 gagatttaaa gctctaaggt aaatataaaa tttttaagtg tataatgtgt taaactactg
1021 attctaattg tttgtgtatt ttagattcca acctatggaa ctgatgaatg ggagcagtgg
1081 tggaatgcct ttaatgagga aaacctgttt tgctcagaag aaatgccatc tagtgatgat
1141 gaggctactg ctgactctca acattctact cctccaaaaa agaagagaaa ggtagaagac
1201 cccaaggact ttccttcaga attgctaagt tttttgagtc atgctgtgtt tagtaataga
1261 actcttgctt gctttgctat ttacaccaca aaggaaaaag ctgcactgct atacaagaaa
1321 attatggaaa aatattctgt aacctttata agtaggcata acagttataa tcataacata
1381 ctgttttttc ttactccaca caggcataga gtgtctgcta ttaataacta tgctcaaaaa
1441 ttgtgtacct ttagcttttt aatttgtaaa ggggttaata aggaatattt gatgtatagt
1501 gccttgacta gagatccatt ttctgttatt gaggaaagtt tgccaggtgg gttaaaggag
1561 catgatttta atccagaaga agcagaggaa actaaacaag tgtcctggaa gcttgtaaca
1621 gagtatgcaa tggaaacaaa atgtgatgat gtgttgttat tgcttgggat gtacttggaa
1681 tttcagtaca gttttgaaat gtgtttaaaa tgtattaaaa aagaacagcc cagccactat
1741 aagtaccatg aaaagcatta tgcaaatgct gctatatttg ctgacagcaa aaaccaaaaa
1801 accatatgcc aacaggctgt tgatactgtt ttagctaaaa agcgggttga tagcctacaa
1861 ttaactagag aacaaatgtt aacaaacaga tttaatgatc ttttggatag gatggatata
1921 atgtttggtt ctacaggctc tgctgacata gaagaatgga tggctggagt tgcttggcta
1981 cactgtttgt tgcccaaaat ggattcagtg gtgtatgact ttttaaaatg catggtgtac
2041 aacattccta aaaaaagata ctggctgttt aaaggaccaa ttgatagtgg taaaactaca
2101 ttagcagctg ctttgcttga attatgtggg gggaaagctt taaatgttaa tttgcccttg
2161 gacaggctga actttgagct aggagtagct attgaccagt ttttagtagt ttttgaggat
2221 gtaaagggca ctggagggga gtccagagat ttgccttcag gtcagggaat taataacctg
2281 gacaatttaa gggattattt ggatggcagt gttaaggtaa acttagaaaa gaaacaccta
2341 aataaaagaa ctcaaatatt tccccctgga atagtcacca tgaatgagta cagtgtgcct
2401 aaaacactgc aggccagatt tgtaaaacaa atagatttta ggcccaaaga ttatttaaag
2461 cattgcctgg aacgcagtga gtttttgtta gaaaagagaa taattcaaag tggcattgct
2521 ttgcttctta tgttaatttg gtacagacct gtggctgagt ttgctcaaag tattcagagc
2581 agaattgtgg agtggaaaga gagattggac aaagagttta gtttgtcagt gtatcaaaaa
2641 atgaagttta atgtggctat gggaattgga gttttagatt ggctaagaaa cagtgatgat
2701 gatgatgaag acagccagga aaatgctgat aaaaatgaag atggtgggga gaagaacatg
2761 gaagactcag ggcatgaaac aggcattgat tcacagtccc aaggctcatt tcaggcccct
2821 cagtcctcac agtctgttca tgatcataat cagccatacc acatttgtag aggttttact
2881 tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt
2941 tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
3001 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
3061 tgtatcttat catgtctgga tcttgcttag gttggctgcc tagttggccc ctgagataaa
3121 gccttgtaat cacatagcct tgcctaatta gtcagaaaac aaaggattaa gtgagacagt
3181 cacaggatat aggaattata aataatacat atattaatag atattcattt tcattacaca
3241 aaagttgcta ttataaatac ttatttgatt gatgagtcta aaaatatatt ccccatataa
3301 ataatgttaa atattaataa atagatttag atttaaaatt caaatattgc aggcaggaca
3361 accattactg ggatgctctt cgacctcgaa acagcatctg actccttttt cgcttccctg
3421 ttttagctgc tggcgacagt tcagccatca cttggatgag ttcatgtatt gctttgcgtt
3481 ggacattcaa gtcagttacc gaataattag tcagcttttc gaagtcatct cgtttctttt
3541 tgttgctatt gaaaaacttg acattcatgt cttccttgat ggtctccaca ctcttttgga
3601 tgctctggtc atctttaaag tttttaaaaa gtttgaagta aaaggagaca atttggctct
3661 gcattatttt tctgtcactc tcctctttcc aattcttcaa aatgcctaag aaaagagttc
3721 cattatccgc tacatctgaa tgacctgcat taaaatattt cttaaggttt tctgcttctt
3781 ttacatatgg gtcctggcag taacagccaa gagaacccaa aacgatgcag agctgaaaag
3841 ccaagatata acttgtatat ttcatcgttt ccgagagaat taagccaaag aagttgaaat
3901 cagtagttct tgtatcaagc tgatccggag cttttagagc agaagtaaca cttccgtaca
3961 ggcctagaag taaaggcaac atccactgag gagcagttct ttgatttgca ccaccaggag
4021 cctcaaattt ttcaataaat tcacctgact gcacattagg actttggctt tgagcttccc
4081 acctctcagt tacttgctga atactgtctg cttcatcaat attatcatag gtgtgcccaa
4141 atgatatttg caacccttcc ctgttggcta cttgtctcac cattgtaggc ctaatgggag
4201 acaaagtaga gtagtaatct tgtaaagagt tataccaatt aacaggagca ttaattactg
4261 tccaagtagt ttcctctaaa aaccttgcca aactgtccct taaatatctt tgggttcttc
4321 tttcaagctc ctgtgaggtg agcctaggaa tgtcattttg tattacacgc caaaaagctt
4381 gagaaatggc attaaaaagt gttggacccc aatgtctggg gtcaagatac tgaacactgt
4441 gaacaaaggt ttgtactcca ggaaataaaa tatcatagta atcatctggc ctatacaaat
4501 ctacagccat tcctggttgt tgatataaac caacagtaga aactttgtga tcccagtcac
4561 taaaaaatct ataccccact tgagcaacag cgctcacacc agtcacagtt tgcagtaaag
4621 ctgcaaatcc agctatagca gcaggagccc cagatatcac agcataggcc tgtggagtga
4681 ggcctatagc agcaattgcc tcagaggttg ttaggccttc aacagtagca acagatgcaa
4741 gttgcacttc aattgcagca gcggcctctc cagcagcaat ttcagctact gaaaatccag
4801 tagcagcagc agcttcagac acagtagcaa ttaggtcccc caacagtgtt aaagcagcac
4861 ccatggacct gaaataaaag acaaaaagac taaacttacc agttaacttt ctggtttttc
4921 agttaacctt tctggttttt tgcgtttccc gtcaacagta tcttcccctt cacaaaattg
4981 cagcaaaagc tctaaaacaa acacaaaaag gcgttgagct gtttttttac tttcagtcca
5041 tgacctacga accttaacgg aggcctggcg tgacagccgg cgcagcacca tggcctgaaa
5101 taacctctga aagaggaact tggttaggta ccttctgagg cggaaagaac cagctgtgga
5161 atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
5221 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca
5281 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc
5341 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
5401 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag
5461 gaggcttttt tggaggccta ggcttttgca aaaagctttg caaagatgga taaagtttta
5521 aacagagagg aatctttgca gctaatggac cttctaggtc ttgaaaggag tgcctggggg
5581 aatattcctc tgatgagaaa ggcatattta aaaaaatgca aggagtttca tcctgataaa
5641 ggaggagatg aagaaaaaat gaagaaaatg aatactctgt acaagaaaat ggaagatgga
5701 gtaaaatatg ctcatcaacc tgactttgga ggcttctggg atgcaactga ggtatttgct
5761 tcttccttaa atcctggtgt tgatgcaatg tactgcaaac aatggcctga gtgtgcaaag
5821 aaaatgtctg ctaactgcat atgcttgctg tgcttactga ggatgaagca tgaaaataga
5881 aaattataca ggaaagatcc acttgtgtgg gttgattgct actgcttcga ttgctttaga
5941 atgtggtttg gacttgatct ttgtgaagga accttacttc tgtggtgtga cataattgga
6001 caaactacct acagagattt aaagctctaa ggtaaatata aaatttttaa gtgtataatg
6061 tgttaaacta ctgattctaa ttgtttgtgt attttagatt ccaacctatg gaactgatga
6121 atgggagcag tggtggaatg cctttaatga ggaaaacctg ttttgctcag aagaaatgcc
6181 atctagtgat gatgaggcta ctgctgactc tcaacattct actcctccaa aaaagaagag
6241 aaaggtagaa gaccccaagg actttccttc agaattgcta agttttttga gtcatgctgt
6301 gtttagtaat agaactcttg cttgctttgc tatttacacc acaaaggaaa aagctgcact
6361 gctatacaag aaaattatgg aaaaatattc tgtaaccttt ataagtaggc ataacagtta
6421 taatcataac atactgtttt ttcttactcc acacaggcat agagtgtctg ctattaataa
6481 ctatgctcaa aaattgtgta cctttagctt tttaatttgt aaaggggtta ataaggaata
6541 tttgatgtat agtgccttga ctagagatcc attttctgtt attgaggaaa gtttgccagg
6601 tgggttaaag gagcatgatt ttaatccaga agaagcagag gaaactaaac aagtgtcctg
6661 gaagcttgta acagagtatg caatggaaac aaaatgtgat gatgtgttgt tattgcttgg
6721 gatgtacttg gaatttcagt acagttttga aatgtgttta aaatgtatta aaaaagaaca
6781 gcccagccac tataagtacc atgaaaagca ttatgcaaat gctgctatat ttgctgacag
6841 caaaaaccaa aaaaccatat gccaacaggc tgttgatact gttttagcta aaaagcgggt
6901 tgatagccta caattaacta gagaacaaat gttaacaaac agatttaatg atcttttgga
6961 taggatggat ataatgtttg gttctacagg ctctgctgac atagaagaat ggatggctgg
7021 agttgcttgg ctacactgtt tgttgcccaa aatggattca gtggtgtatg actttttaaa
7081 atgcatggtg tacaacattc ctaaaaaaag atactggctg tttaaaggac caattgatag
7141 tggtaaaact acattagcag ctgcctcgcg cgtttcggtg atgacggtga aaacctctga
7201 cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa
7261 gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat gacccagtca
7321 cgtagcgata gcggagtgta tactggctta actatgcggc atcagagcag attgtactga
7381 gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca
7441 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
7501 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
7561 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
7621 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
7681 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
7741 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
7801 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
7861 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
7921 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
7981 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
8041 ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc
8101 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
8161 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
8221 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
8281 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
8341 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
8401 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
8461 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
8521 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
8581 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
8641 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
8701 ctgcaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
8761 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
8821 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
8881 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
8941 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
9001 caacacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
9061 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
9121 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
9181 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
9241 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
9301 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
9361 cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa
9421 ataggcgtat cacgaggccc tttcgtcttc aagaa
//