LOCUS LT727632 7949 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian expression vector pBM6DraA6, complete sequence.
ACCESSION LT727632
VERSION LT727632.1
KEYWORDS .
SOURCE Mammalian expression vector pBM6DraA6
ORGANISM Mammalian expression vector pBM6DraA6
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 7949)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..7949
/organism="Mammalian expression vector pBM6DraA6"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 2207."
/db_xref="taxon:1944752"
rep_origin complement(36..111)
/note="SV40 ori"
misc_feature complement(170..1005)
/note="PY"
rep_origin complement(262..373)
/note="PY-O"
regulatory complement(1066..1162)
/note="polyA"
/regulatory_class="terminator"
regulatory 1066..1162
/note="polyA"
/regulatory_class="terminator"
misc_feature complement(1163..1714)
/note="3' UTR of SV40 small t-antigen"
misc_feature complement(1715..1781)
/note="intron of SV40 small t-antigen"
CDS complement(1782..>1856)
/codon_start=1
/transl_table=11
/note="part of SV40 small t-antigen"
/protein_id="SJL89035.1"
/translation="DLCEGTLLLWCDIIGQTTYRDLKL"
CDS complement(2201..2995)
/transl_table=11
/note="neomycin resistance"
/protein_id="SJL89036.1"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
regulatory complement(3354..3536)
/note="LTR"
/regulatory_class="promoter"
regulatory complement(4001..4097)
/note="polyA"
/regulatory_class="terminator"
regulatory 4001..4097
/note="polyA"
/regulatory_class="terminator"
misc_feature complement(4098..4649)
/note="3' UTR of SV40 small t-antigen"
misc_feature complement(4650..4716)
/note="intron of SV40 small t-antigen"
CDS complement(4717..>4791)
/codon_start=1
/transl_table=11
/note="part of SV40 small t-antigen"
/protein_id="SJL89037.1"
/translation="DLCEGTLLLWCDIIGQTTYRDLKL"
regulatory 4799..4815
/note="SP6"
/regulatory_class="promoter"
CDS complement(5022..5657)
/transl_table=11
/note="mIL6"
/protein_id="SJL89038.1"
/translation="MKFLSARDFHPVAFLGLMLVTTTAFPTSQVRRGDFTEDTTPNRP
VYTTSQVGGLITHVLWEIVEMRKELCNGNSDCMNNDDALAENNLKLPEIQRNDGCYQT
GYNQEICLLKISSGLLEYHSYLEYMKNNLKDNKKDKARVLQRDTETLIHIFNQEVKDL
HKIVLPTPISNALLTDKLESQKEWLRTKTIQFILKSLEEFLKVTLRSTRQT"
regulatory complement(5763..5779)
/note="T7"
/regulatory_class="promoter"
regulatory complement(5825..6411)
/note="hCMV"
/regulatory_class="promoter"
misc_feature complement(6563..6765)
/note="supF"
rep_origin 6889..7347
/note="M13-O"
rep_origin complement(7366..7598)
/note="pMB1 ori"
BASE COUNT 2042 a 1918 c 1855 g 2134 t
ORIGIN
1 gaattagctt tttgcaaaag cctaggcctc caaaaaagcc tcctcactac ttctggaata
61 gctcagaggc cgaggcggcc tcggcctctg cataaataaa aaaaattagt cagccatggg
121 gcggagaatg ggcggaactg ggcggagtta ggggcgggat gggcggagta agacccgcac
181 ccgttcccaa actgcttatt aaagggggta tggaggtgct ggaccttgtg acagggccag
241 acagtgtgac agaaatagaa gcagtcctgt tttgacaagt tgcctctgga agcctctaca
301 atgcctctct tctttttctc cagagtaagc gggccagggg tccccggcct ctgcttaata
361 ctaaaaaaaa cagctgttgt catagtaatg attgggtgga aacattctag gcctgggtgg
421 agaggctttt gcttcctctt gcaaaaccac acgccctctg gagggcgttg cctagcaact
481 aattaaaaga ggatgtcgca cggccagctg cgtcagttag tccacttcct gcttaactga
541 cttgacattt tctattttaa gagtcgggag gaaaattact gtgttggagg ccctccgcca
601 tcttctgaag ctgatcaagt aagtgaattt tcaaaatggg agccgcactg actattctag
661 tagacctcat cgagggatta gctgaagtgt ctacccttac gggactctcg gcagaagcta
721 ttttatctgg agaagccctc gctgcccttg atggcgaaat tacagctctg actttggagg
781 gtgtaatgag ttcggagaca gccctagcaa ctatgggtat ttcagaggag gtgtatgggt
841 ttgtcagtac tgtgcctgtg tttgtaagtc gaacagcggg ggctatatgg ctgatgcaga
901 cagttcaagg tgcctctact atttccctag gaatacagcg gtacctacac aacgaagagg
961 tccctactgt aaatagaaat atggcgttga taccatggcg ggatcgatcc agacatgata
1021 agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt
1081 tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt
1141 aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg ggaggttttt
1201 taaagcaagt aaaacctcta caaatgtggt atggctgatt atgatctcta gtcaaggcac
1261 tatacatcaa atattcctta ttaacccctt tacaaattaa aaagctaaag gtacacaatt
1321 tttgagcata gttattaata gcagacactc tatgcctgtg tggagtaaga aaaaacagta
1381 tgttatgatt ataactgtta tgcctactta taaaggttac agaatatttt tccataattt
1441 tcttgtatag cagtgcagct ttttcctttg tggtgtaaat agcaaagcaa gcaagagttc
1501 tattactaaa cacagcatga ctcaaaaaac ttagcaattc tgaaggaaag tccttggggt
1561 cttctacctt tctcttcttt tttggaggag tagaatgttg agagtcagca gtagcctcat
1621 catcactaga tggcatttct tctgagcaaa acaggttttc ctcattaaag gcattccacc
1681 actgctccca ttcatcagtt ccataggttg gaatctaaaa tacacaaaca attagaatca
1741 gtagtttaac acattataca cttaaaaatt ttatatttac cttagagctt taaatctctg
1801 taggtagttt gtccaattat gtcacaccac agaagtaagg ttccttcaca aagatccggg
1861 accaaagcgg ccatcgtgcc tccccactcc tgcagttcgg gggcatggat gcgcggatag
1921 ccgctgctgg tttcctggat gccgacggat ttgcactgcc ggtagaactc cgcgaggtcg
1981 tccagcctca ggcagcagct gaaccaactc gcgaggggat cgagcccggg gtgggcgaag
2041 aactccagca tgagatcccc gcgctggagg atcatccagc cggcgtcccg gaaaacgatt
2101 ccgaagccca acctttcata gaaggcggcg gtggaatcga aatctcgtga tggcaggttg
2161 ggcgtcgctt ggtcggtcat ttcgaacccc agagtcccgc tcagaagaac tcgtcaagaa
2221 ggcgatagaa ggcgatgcgc tgcgaatcgg gagcggcgat accgtaaagc acgaggaagc
2281 ggtcagccca ttcgccgcca agctcttcag caatatcacg ggtagccaac gctatgtcct
2341 gatagcggtc cgccacaccc agccggccac agtcgatgaa tccagaaaag cggccatttt
2401 ccaccatgat attcggcaag caggcatcgc catgggtcac gacgagatcc tcgccgtcgg
2461 gcatgcgcgc cttgagcctg gcgaacagtt cggctggcgc gagcccctga tgctcttcgt
2521 ccagatcatc ctgatcgaca agaccggctt ccatccgagt acgtgctcgc tcgatgcgat
2581 gtttcgcttg gtggtcgaat gggcaggtag ccggatcaag cgtatgcagc cgccgcattg
2641 catcagccat gatggatact ttctcggcag gagcaaggtg agatgacagg agatcctgcc
2701 ccggcacttc gcccaatagc agccagtccc ttcccgcttc agtgacaacg tcgagcacag
2761 ctgcgcaagg aacgcccgtc gtggccagcc acgatagccg cgctgcctcg tcctgcagtt
2821 cattcagggc accggacagg tcggtcttga caaaaagaac cgggcgcccc tgcgctgaca
2881 gccggaacac ggcggcatca gagcagccga ttgtctgttg tgcccagtca tagccgaata
2941 gcctctccac ccaagcggcc ggagaacctg cgtgcaatcc atcttgttca atcatgcgaa
3001 acgatcctca tcctgtctct tgatcagatc ttgatcccct gcgccatcag atccttggcg
3061 gcaagaaagc catccagttt actttgcagg gcttcccaac cttaccagag ggcgccccag
3121 ctggcaattc cggttcgctt gctgtccata aaaccgccca gtctagctat cgccatgtaa
3181 gcccactgca agctacctgc tttctctttg cgcttgcgtt ttcccttgtc cagatagccc
3241 agtagctgac attcatccgg ggtcagcacc gtttctgcgg actggctttc tacgtgttcc
3301 gcttccttta gcagcccttg cgccctgagt gcttgcggca gcgtgaagct tggaggtgca
3361 caccaatgtg gtgaatggtc aaatggcgtt tattgtatcg agctaggcac ttaaatacaa
3421 ttatctctgc aatgcggaat tcagtggttc gtccaatcca tgtcagacct gtctgttgcc
3481 ttcctaataa ggcacgatcg taccacctta cttccaccaa tcggcatgca cggtgctttt
3541 tctctccttg taaggcatgt tgctaactca tcgttaccat gttgcaagac tacaagtgta
3601 ttgcataaga ctacatttcc ccctccctat gcaaaagcga aactactata tcctgagggg
3661 actcctaacc gcgtacaacc gaagccccgc ttttcgccta aacacaccct agtcccctca
3721 gatacgcgta tatctggccc gtacatcgcg aagcagcgca aaacgcctaa ccctaagcag
3781 attcttcatg caattgtcgg tcaagccttg ccttgttgta gcttaaattt tgctcgcgca
3841 ctactcagcg acctccaaca cacaagcagg gagcagatac tggcttaact atgcggcatc
3901 agagcagatt gtactgagag tgcaccatag gggatcggga gatccagaca tgataagata
3961 cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga
4021 aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa
4081 caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag
4141 caagtaaaac ctctacaaat gtggtatggc tgattatgat ctctagtcaa ggcactatac
4201 atcaaatatt ccttattaac ccctttacaa attaaaaagc taaaggtaca caatttttga
4261 gcatagttat taatagcaga cactctatgc ctgtgtggag taagaaaaaa cagtatgtta
4321 tgattataac tgttatgcct acttataaag gttacagaat atttttccat aattttcttg
4381 tatagcagtg cagctttttc ctttgtggtg taaatagcaa agcaagcaag agttctatta
4441 ctaaacacag catgactcaa aaaacttagc aattctgaag gaaagtcctt ggggtcttct
4501 acctttctct tcttttttgg aggagtagaa tgttgagagt cagcagtagc ctcatcatca
4561 ctagatggca tttcttctga gcaaaacagg ttttcctcat taaaggcatt ccaccactgc
4621 tcccattcat cagttccata ggttggaatc taaaatacac aaacaattag aatcagtagt
4681 ttaacacatt atacacttaa aaattttata tttaccttag agctttaaat ctctgtaggt
4741 agtttgtcca attatgtcac accacagaag taaggttcct tcacaaagat cctctagcat
4801 ttaggtgaca ctatagaata gggccctcta gatgcatgct cgagcggccg ccagtgtgat
4861 ggataaatta tcaataaatt aaaaataatt aaaatagtgt cccaacattc atattgtcag
4921 ttcttcgtag agaacaacat aagtcagata cctgacaaca ggatatattt tctgaccaca
4981 gtgaggaatg tccacaaact gatatgctta ggcataacgc actaggtttg ccgagtagat
5041 ctcaaagtga cttttagaaa ttcttcaagt gatttcaaga tgaattggat ggtcttggtc
5101 cttagccact ccttctgtga ctccagctta tctgttagga gagcattgga aattggggta
5161 ggaaggacta ttttatgtaa atcttttacc tcttggttga agatatgaat tagagtttct
5221 gtatctctct gaaggactct ggctttgtct ttcttgttat cttttaagtt gttcttcatg
5281 tactccaggt agctatggta ctccagaaga ccagaggaaa ttttcaatag gcaaatttcc
5341 tgattatatc cagtttggta gcatccatca tttctttgta tctctggaag tttcagattg
5401 ttttctgcaa gtgcatcatc gttgttcata caatcagaat tgccattgca caactctttt
5461 ctcatttcca cgatttccca gagaacatgt gtaattaagc ctccgacttg tgaagtggta
5521 tagacaggtc tgttgggagt ggtatcctct gtgaagtctc ctctccggac ttgtgaagta
5581 gggaaggccg tggttgtcac cagcatcagt cccaagaagg caactggatg gaagtctctt
5641 gcagagagga acttcatagc ggtttctgga attgactatc gttcttggtg atctgcagaa
5701 ttccagcaca ctggcggccg ttactagtgg atccgagctc ggtaccaagc ttccggtctc
5761 cctatagtga gtcgtattaa tttcgataag ccagttaagc agtgggttct ctagttagcc
5821 agagagctct gcttatatag acctcccacc gtacacgcct accgcccatt tgcgtcaatg
5881 gggcggagtt gttacgacat tttggaaagt cccgttgatt ttggtgccaa aacaaactcc
5941 cattgacgtc aatggggtgg agacttggaa atccccgtga gtcaaaccgc tatccacgcc
6001 cattgatgta ctgccaaaac cgcatcacca tggtaatagc gatgactaat acgtagatgt
6061 actgccaagt aggaaagtcc cataaggtca tgtactgggc ataatgccag gcgggccatt
6121 taccgtcatt gacgtcaata gggggcgtac ttggcatatg atacacttga tgtactgcca
6181 agtgggcagt ttaccgtaaa tactccaccc attgacgtca atggaaagtc cctattggcg
6241 ttactatggg aacatacgtc attattgacg tcaatgggcg ggggtcgttg ggcggtcagc
6301 caggcgggcc atttaccgta agttatgtaa cgcggaactc catatatggg ctatgaacta
6361 atgaccccgt aattgattac tattaataac tagtcaataa tcaatgtcaa cgcgtatatc
6421 tggcccgtac atcgcgaagc agcgcaaaac gcctaaccct aagcagattc ttcatgcaat
6481 tgtcggtcaa gccttgcctt gttgtagctt aaattttact cgcgcactac tcagcgacct
6541 ccaacacaca agcagggagc agattctttc ggacttttga aagtgatggt ggtgggggaa
6601 ggattcgaac cttcgaagtc gatgacggca gatttagagt ctgctccctt tggccgctcg
6661 ggaaccccac cacgggtaat gcttttactg gcctgctccc ttatcgggaa gcggggcgca
6721 tcatatcaaa tgacgcgccg ctgtaaagtg ttacgttgag aaagaccgcg gtgatccagc
6781 tggcgtaccg ttcctgtcta aaatcccttt aatcggcctc ctgtttagct cccgctctga
6841 ttccaacgag gaaagcacgt tatacggtct cgtcaaagca accatagtac gcgccctgta
6901 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
6961 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
7021 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
7081 acctcgaccc caaaaaactt gatttgggtg atggttcacg tagtgggcgg ccatcgccct
7141 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
7201 tccaaactgg aacaacactc aaccctatct cgggctattc ttttgattta taagggattt
7261 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
7321 ttaacaaaat attaacgttt acaatttcta gctagaagct agcttgcgtt gctggcgttt
7381 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
7441 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
7501 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
7561 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
7621 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
7681 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
7741 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
7801 aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc
7861 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
7921 ttttttgttt gcaagcagca gattacgcc
//