LOCUS LT727628 5117 bp DNA circular SYN 06-FEB-2017 DEFINITION Mammalian expression vector pSV23SMTNF, complete sequence. ACCESSION LT727628 VERSION LT727628.1 KEYWORDS . SOURCE Mammalian expression vector pSV23SMTNF ORGANISM Mammalian expression vector pSV23SMTNF other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 5117) AUTHORS De Schamphelaire W. JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent, Technologiepark 927, 9052, BELGIUM. REFERENCE 2 AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K., Roggeman Fonseca M., Vanhoucke M., Beyaert R. TITLE BCCM/LMBP Plasmid collection JOURNAL Unpublished. FEATURES Location/Qualifiers source 1..5117 /organism="Mammalian expression vector pSV23SMTNF" /mol_type="other DNA" /note="BCCM/LMBP Plasmid collection (Ghent University,Belgium) accession number LMBP 2171." /db_xref="taxon:1945078" CDS <1..809 /codon_start=3 /transl_table=11 /note="part of SV40 VP1" /protein_id="SJL89019.1" /translation="PLPNLNEDLTCGNILMWEAVTVKTEVIGVTAMLNLHSGTQKTHE NGAGKPIQGSNFHFFAVGGEPLELQGVLANYRTKYPAQTVTPKNATVDSQQMNTDHKA VLDKDNAYPVECWVPDPSKNENTRYFGTYTGGENVPPVLHITNTATTVLLDEQGVGPL CKADSLYVSAVDICGLFTNTSGTQQWKGLPRYFKITLRKRSVKNPYPISFLLSDLINR RTQRVDGQPMIGMSSQVEEVRVYEDTEELPGDPDMIRYIDEFGQTTTRMQ" regulatory complement(810..906) /note="SV40 polyA early" /regulatory_class="terminator" regulatory 810..906 /note="SV40 polyA late" /regulatory_class="terminator" misc_feature complement(907..1458) /note="3' UTR of SV40 small t-antigen" misc_feature complement(1459..1525) /note="intron of SV40 small t-antigen" CDS complement(1526..>1600) /codon_start=1 /transl_table=11 /note="part of SV40 small t-antigen" /protein_id="SJL89020.1" /translation="DLCEGTLLLWCDIIGQTTYRDLKL" misc_feature complement(1624..1781) /note="3' UTR of mTNF" CDS complement(1782..>2252) /codon_start=1 /transl_table=11 /note="mTNF mature sequence" /protein_id="SJL89021.1" /translation="LRSSSQNSSDKPVAHVVANHQVEEQLEWLSQRANALLANGMDLK DNQLVVPADGLYLVYSQVLFKGQGCPDYVLLTHTVSRFAISYQEKVNLLSAVKSPCPK DTPEGAELKPWYEPIYLGGVFQLEKGDQLSAEVNLPKYLDFAESGQVYFGVIAL" CDS complement(<2253..2489) /transl_table=11 /note="mTNF presequence" /protein_id="SJL89022.1" /translation="MSTESMIRDVELAEEALPQKMGGFQNSRRCLCLSLFSFLLVAGA TTLFCLLNFGVIGPQRDEKFPNGLPLISSMAQTLT" misc_feature complement(2490..2517) /note="5' UTR of mTNF" rep_origin complement(2571..2646) /note="SV40 ori" regulatory complement(2603..2884) /note="SV40 early promoter" /regulatory_class="promoter" rep_origin complement(3106..3508) /note="pMB1 ori" CDS complement(4049..4909) /transl_table=11 /note="ampicillin resistance" /protein_id="SJL89023.1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" BASE COUNT 1338 a 1159 c 1283 g 1337 t ORIGIN 1 ttcctttgcc taatttaaat gaggacttaa cctgtggaaa tattttgatg tgggaagctg 61 ttactgttaa aactgaggtt attggggtaa ctgctatgtt aaacttgcat tcagggacac 121 aaaaaactca tgaaaatggt gctggaaaac ccattcaagg gtcaaatttt catttttttg 181 ctgttggtgg ggaacctttg gagctgcagg gtgtgttagc aaactacagg accaaatatc 241 ctgctcaaac tgtaacccca aaaaatgcta cagttgacag tcagcagatg aacactgacc 301 acaaggctgt tttggataag gataatgctt atccagtgga gtgctgggtt cctgatccaa 361 gtaaaaatga aaacactaga tattttggaa cctacacagg tggggaaaat gtgcctcctg 421 ttttgcacat tactaacaca gcaaccacag tgcttcttga tgagcagggt gttgggccct 481 tgtgcaaagc tgacagcttg tatgtttctg ctgttgacat ttgtgggctg tttaccaaca 541 cttctggaac acagcagtgg aagggacttc ccagatattt taaaattacc cttagaaagc 601 ggtctgtgaa aaacccctac ccaatttcct ttttgttaag tgacctaatt aacaggagga 661 cacagagggt ggatgggcag cctatgattg gaatgtcctc tcaagtagag gaggttaggg 721 tttatgagga cacagaggag cttcctgggg atccagacat gataagatac attgatgagt 781 ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg 841 ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca 901 ttcattttat gtttcaggtt cagggggagg tgtgggaggt tttttaaagc aagtaaaacc 961 tctacaaatg tggtatggct gattatgatc tctagtcaag gcactataca tcaaatattc 1021 cttattaacc cctttacaaa ttaaaaagct aaaggtacac aatttttgag catagttatt 1081 aatagcagac actctatgcc tgtgtggagt aagaaaaaac agtatgttat gattataact 1141 gttatgccta cttataaagg ttacagaata tttttccata attttcttgt atagcagtgc 1201 agctttttcc tttgtggtgt aaatagcaaa gcaagcaaga gttctattac taaacacagc 1261 atgactcaaa aaacttagca attctgaagg aaagtccttg gggtcttcta cctttctctt 1321 cttttttgga ggagtagaat gttgagagtc agcagtagcc tcatcatcac tagatggcat 1381 ttcttctgag caaaacaggt tttcctcatt aaaggcattc caccactgct cccattcatc 1441 agttccatag gttggaatct aaaatacaca aacaattaga atcagtagtt taacacatta 1501 tacacttaaa aattttatat ttaccttaga gctttaaatc tctgtaggta gtttgtccaa 1561 ttatgtcaca ccacagaagt aaggttcctt cacaaagatc cgtcgaccgg gggggggggg 1621 ggggttgaaa gctctgagca cagagttgga ctctgagcca taatcccctt tctaagttag 1681 aaggacacag actgggggct ctgaggagta gacaataaag gggtcagagt aaaggggtca 1741 gagtgggggc tgggtagaga atggatgaac acccattccc ttcacagagc aatgactcca 1801 aagtagacct gcccggactc cgcaaagtct aagtacttgg gcagattgac ctcagcgctg 1861 agttggtccc ccttctccag ctggaagact cctcccaggt atatgggctc ataccagggt 1921 ttgagctcag ccccctcagg ggtgtccttg gggcaggggc tcttgacggc agagaggagg 1981 ttgactttct cctggtatga gatagcaaat cggctgacgg tgtgggtgag gagcacgtag 2041 tcggggcagc cttgtccctt gaagagaacc tgggagtaga caaggtacaa cccatcggct 2101 ggcaccacta gttggttgtc tttgagatcc atgccgttgg ccaggagggc gttggcgcgc 2161 tggctcagcc actccagctg ctcctccact tggtggtttg ctacgacgtg ggctacaggc 2221 ttgtcactcg aattttgaga agatgatctg agtgtgaggg tctgggccat agaactgatg 2281 agagggaggc catttgggaa cttctcatcc ctttggggac cgatcacccc gaagttcagt 2341 agacagaaga gcgtggtggc ccctgccaca agcaggaatg agaagaggct gagacatagg 2401 caccgcctgg agttctggaa gccccccatc ttttggggga gtgcctcttc tgccagttcc 2461 acgtcgcgga tcatgctttc tgtgctcatg gtgtcttttc tggagggaga tgtggcgggt 2521 cgactctaga gccaagcttg agctttttgc aaaagcctag gcctccaaaa aagcctcctc 2581 actacttctg gaatagctca gaggccgagg cggcctcggc ctctgcataa ataaaaaaaa 2641 ttagtcagcc atggggcgga gaatgggcgg aactgggcgg agttaggggc gggatgggcg 2701 gagttagggg cgggactatg gttgctgact aattgagatg catgctttgc atacttctgc 2761 ctgctgggga gcctggggac tttccacacc tggttgctga ctaattgaga tgcatgcttt 2821 gcatacttct gcctgctggg gagcctgggg actttccaca ccctaactga cacacattcc 2881 acagtcgggc agcgttgggt cctggccacg ggtgcgcatg atcgtgctcc tgtcgttgag 2941 gacccggcta ggctggcggg gttgccttac tggttagcag aatgaatcac cgatacgcga 3001 gcgaacgtga agcgactgct gctgcaaaac gtctgcgacc tgagcaacaa catgaatggt 3061 cttcggtttc cgtgtttcgt aaagtctgga aacgcggaag tcagcgctct tccgcttcct 3121 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa 3181 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa 3241 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc 3301 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga 3361 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc 3421 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt 3481 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct 3541 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg 3601 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta 3661 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct 3721 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa 3781 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt 3841 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta 3901 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat 3961 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa 4021 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct 4081 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta 4141 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct 4201 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg 4261 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa 4321 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctgcaggc atcgtggtgt 4381 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta 4441 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca 4501 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta 4561 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct 4621 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaacacgg gataataccg 4681 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac 4741 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact 4801 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa 4861 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt 4921 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat 4981 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg 5041 acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc 5101 cctttcgtct tcaagaa //