LOCUS LT727626 3439 bp DNA circular SYN 06-FEB-2017
DEFINITION Vector pSP64Mx, complete sequence.
ACCESSION LT727626
VERSION LT727626.1
KEYWORDS .
SOURCE Vector pSP64Mx
ORGANISM Vector pSP64Mx
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 3439)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..3439
/organism="Vector pSP64Mx"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 2164."
/db_xref="taxon:1945401"
regulatory 13..458
/note="Mx"
/regulatory_class="promoter"
regulatory complement(544..627)
/note="lac"
/regulatory_class="promoter"
rep_origin complement(715..1125)
/note="pMB1 ori"
CDS complement(1662..2522)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL89016.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
regulatory 3417..3433
/note="SP6"
/regulatory_class="promoter"
BASE COUNT 845 a 874 c 891 g 829 t
ORIGIN
1 aagcttgggc tgcagtagca gtgctcagac ctctaaacct gtagtcacaa gggacccaga
61 agggctggca gggctcctca gtgattctgg tatggtttgt ctctgaccct tcctagtgca
121 gctggaagaa tccagtgaaa agagaaagtt agtctaggtt gtgttcaact gagaggaggt
181 caggctgaag gctcagcaca gagctggaag ggtcctggga aagcaggcag ccatctgaac
241 tggtgtccat actgcatgtg tcttggtggg ctgagcctac aatatcgatt tcccaacctc
301 agtaccaagc caaggcccct cagggtgagt ttcgtttctg agctcctttc atatgtggtg
361 ggggtgtggg cacagcaagc tcaggctttt tcacggtttc aattctcctc tggaaggggc
421 ggagccagca ctatttaaag aggtctgagc cctggcagga ctctagagga tccccgggcg
481 agctcgaatt cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
541 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
601 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
661 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
721 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
781 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
841 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
901 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
961 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
1021 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
1081 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
1141 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
1201 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
1261 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
1321 cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt
1381 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
1441 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
1501 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
1561 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
1621 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
1681 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
1741 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
1801 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
1861 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
1921 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
1981 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
2041 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
2101 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
2161 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
2221 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
2281 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
2341 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
2401 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
2461 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
2521 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
2581 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
2641 aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg
2701 cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac
2761 atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc
2821 cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta tgcggcatca
2881 gagcagattg tactgagagt gcaccatatc gacgctctcc cttatgcgac tcctgcatta
2941 ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat
3001 gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga
3061 aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga
3121 tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt
3181 agaggatctg gctagcgatg accctgctga ttggttcgct gaccatttcc ggggtgcgga
3241 acggcgttac cagaaactca gaaggttcgt ccaaccaaac cgactctgac ggcagtttac
3301 gagagagatg atagggtctg cttcagtaag ccagatgcta cacaattagg cttgtacata
3361 ttgtcgttag aacgcggcta caattaatac ataaccttat gtatcataca catacgattt
3421 aggtgacact atagaatac
//