LOCUS LT727624 5724 bp DNA circular SYN 06-FEB-2017 DEFINITION Mammalian expression vector pSV23SE6Hm, complete sequence. ACCESSION LT727624 VERSION LT727624.1 KEYWORDS . SOURCE Mammalian expression vector pSV23SE6Hm ORGANISM Mammalian expression vector pSV23SE6Hm other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 5724) AUTHORS De Schamphelaire W. JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent, Technologiepark 927, 9052, BELGIUM. REFERENCE 2 AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K., Roggeman Fonseca M., Vanhoucke M., Beyaert R. TITLE BCCM/LMBP Plasmid collection JOURNAL Unpublished. FEATURES Location/Qualifiers source 1..5724 /organism="Mammalian expression vector pSV23SE6Hm" /mol_type="other DNA" /note="BCCM/LMBP Plasmid collection (Ghent University,Belgium) accession number LMBP 2153." /db_xref="taxon:1945069" CDS <1..809 /codon_start=3 /transl_table=11 /note="part of SV40 VP1" /protein_id="SJL89008.1" /translation="PLPNLNEDLTCGNILMWEAVTVKTEVIGVTAMLNLHSGTQKTHE NGAGKPIQGSNFHFFAVGGEPLELQGVLANYRTKYPAQTVTPKNATVDSQQMNTDHKA VLDKDNAYPVECWVPDPSKNENTRYFGTYTGGENVPPVLHITNTATTVLLDEQGVGPL CKADSLYVSAVDICGLFTNTSGTQQWKGLPRYFKITLRKRSVKNPYPISFLLSDLINR RTQRVDGQPMIGMSSQVEEVRVYEDTEELPGDPDMIRYIDEFGQTTTRMQ" regulatory complement(810..906) /note="polyA" /regulatory_class="terminator" regulatory 810..906 /note="polyA" /regulatory_class="terminator" misc_feature complement(907..1458) /note="3' UTR of SV40 small t-antigen" misc_feature complement(1459..1525) /note="intron of SV40 small t-antigen" CDS complement(1526..>1600) /codon_start=1 /transl_table=11 /note="part of SV40 small t-antigen" /protein_id="SJL89009.1" /translation="DLCEGTLLLWCDIIGQTTYRDLKL" misc_feature complement(1613..1703) /note="3' UTR of mIghg2b" CDS complement(1704..3131) /transl_table=11 /note="mIghg2b" /note="heavy chain of mouse anti-hPLAP monoclonal antibody (E6H)" /protein_id="SJL89010.1" /translation="MEWIWIFLFILSGTAGVRYQVQLQQSGAELARPGASVKLSCKAS GYTLTSYGISWVKQRTGQGLEWIGEIYPGSGNSYFNEKFKGKATLTVDKSSSTAYLHL SSLTSEDSAVYFCAGPRQVGLLPFGYWGQGTLVTASAAKTTPPSVYPLAPGCGDTTGS SVTLGCLVKGYFPESVTVTWNSGSLSSSVHTFPALLQSGLYTMSSSVTVPSSTWPSQT VTCSVAHPASSTTVDKKLEPSGPTSTINPCPPCKECHKCPAPNLEGGPSVFIFPPNIK DVLMISLTPKVTCVVVDVSEDDPDVQISWFVNNVEVLTAQTQTHREDYNSTIRVVSAL PIQHQDWMSGKEFKCKVNNKDLPAPIERTISKIKGIVRAPQVYILSPPPEQLSRKDVS LTCLAVGFSPEDISVEWTSNGHTEENYKDTAPVLDSDGSYFIYSKLNMKTSKWEKTDS FSCNVRHEGLKNYYLKKTISRSPGK" rep_origin complement(3178..3253) /note="SV40 ori" regulatory complement(3210..3491) /note="SV40 early promoter" /regulatory_class="promoter" rep_origin complement(3710..4115) /note="pMB1 ori" CDS complement(4656..5516) /transl_table=11 /note="ampicillin resistance" /protein_id="SJL89011.1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" BASE COUNT 1467 a 1313 c 1411 g 1533 t ORIGIN 1 ttcctttgcc taatttaaat gaggacttaa cctgtggaaa tattttgatg tgggaagctg 61 ttactgttaa aactgaggtt attggggtaa ctgctatgtt aaacttgcat tcagggacac 121 aaaaaactca tgaaaatggt gctggaaaac ccattcaagg gtcaaatttt catttttttg 181 ctgttggtgg ggaacctttg gagctgcagg gtgtgttagc aaactacagg accaaatatc 241 ctgctcaaac tgtaacccca aaaaatgcta cagttgacag tcagcagatg aacactgacc 301 acaaggctgt tttggataag gataatgctt atccagtgga gtgctgggtt cctgatccaa 361 gtaaaaatga aaacactaga tattttggaa cctacacagg tggggaaaat gtgcctcctg 421 ttttgcacat tactaacaca gcaaccacag tgcttcttga tgagcagggt gttgggccct 481 tgtgcaaagc tgacagcttg tatgtttctg ctgttgacat ttgtgggctg tttaccaaca 541 cttctggaac acagcagtgg aagggacttc ccagatattt taaaattacc cttagaaagc 601 ggtctgtgaa aaacccctac ccaatttcct ttttgttaag tgacctaatt aacaggagga 661 cacagagggt ggatgggcag cctatgattg gaatgtcctc tcaagtagag gaggttaggg 721 tttatgagga cacagaggag cttcctgggg atccagacat gataagatac attgatgagt 781 ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg 841 ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca 901 ttcattttat gtttcaggtt cagggggagg tgtgggaggt tttttaaagc aagtaaaacc 961 tctacaaatg tggtatggct gattatgatc tctagtcaag gcactataca tcaaatattc 1021 cttattaacc cctttacaaa ttaaaaagct aaaggtacac aatttttgag catagttatt 1081 aatagcagac actctatgcc tgtgtggagt aagaaaaaac agtatgttat gattataact 1141 gttatgccta cttataaagg ttacagaata tttttccata attttcttgt atagcagtgc 1201 agctttttcc tttgtggtgt aaatagcaaa gcaagcaaga gttctattac taaacacagc 1261 atgactcaaa aaacttagca attctgaagg aaagtccttg gggtcttcta cctttctctt 1321 cttttttgga ggagtagaat gttgagagtc agcagtagcc tcatcatcac tagatggcat 1381 ttcttctgag caaaacaggt tttcctcatt aaaggcattc caccactgct cccattcatc 1441 agttccatag gttggaatct aaaatacaca aacaattaga atcagtagtt taacacatta 1501 tacacttaaa aattttatat ttaccttaga gctttaaatc tctgtaggta gtttgtccaa 1561 ttatgtcaca ccacagaagt aaggttcctt cacaaagatc cgtcgactct agcaggcttt 1621 gctggatgct ttatttatac aagggatgca tggatatggg tgccagtgtc tcttaggacc 1681 tgagagcttt gtgggtgctg agctcattta cccggagacc gggagatggt cttcttcagg 1741 tagtaatttt tcagaccctc gtgtctcacg ttgcatgaga aggaatctgt tttctcccac 1801 ttgcttgttt tcatattgag cttgctatat atgaagtaag aaccgtcaga gtctaggact 1861 ggtgcggtgt ccttgtagtt ctcctctgta tgcccattgc tggtccactc cacactgatg 1921 tcttcagggc tgaagcccac ggccaggcaa gtcagactga catctttcct ggacaactgc 1981 tctggtggtg gcgacaagat gtatacttgt ggagctctga ctatcccttt aatttttgag 2041 atggttctct caatgggcgc tgggaggtct ttgttgttga ccttgcattt gaactccttg 2101 ccactcatcc agtcctggtg ctggatgggg agggcactga ccacccggat agtactgttg 2161 taatcctctc tatgggtttg tgtctgagct gtgagtactt ccacgttgtt cacaaaccag 2221 ctgatctgga cgtctgggtc atcctcgctc acatccacca ccacacacgt gaccttgggt 2281 gtcagggaga tcatgagtac atccttgata tttggaggga agatgaagac ggatggtcca 2341 ccctcgaggt taggagctgg gcatttgtga cactccttgc atggaggaca ggggttgatt 2401 gttgaagtgg gcccgctggg ctcaagtttt ttgtccaccg tggtgctgct ggctgggtga 2461 gcaacactgc aggtaacggt ctggcttggc caggtgctgg aggggacagt cactgagctg 2521 ctcatagtgt agagtccaga ctgcaggaga gctgggaagg tgtgcacact gctggacagg 2581 gatccggagt tccaagtcac agtcactgac tcagggaagt agcccttgac caggcatccc 2641 agagtcacgg aggaaccagt tgtatctcca cacccagggg ccagtggata gactgatggg 2701 ggtgttgttt tggctgcaga ggcagtgacc agagtccctt ggccccagta accaaaaggg 2761 agtagcccga cctgtctagg tccggcacag aaatagacgg cagagtcctc agatgtcagg 2821 ctgctgaggt gcaagtaggc tgtgctggag gatttgtcta cagtcagtgt ggccttgccc 2881 ttaaacttct cattgaagta cgaatttcca cttccaggat aaatttctcc aatccactca 2941 aggccctgtc cagttctctg cttcacccaa cttataccat aacttgtgag ggtgtatcca 3001 gaggccttgc aggacagctt cactgaggcc ccaggcctcg ccagctcagc tccagactgc 3061 tgcagctgaa cctggtaccg gacacctgca gttcctgaca ggatgaagag aaagatccag 3121 atccattcca tgctagagcc aagcttgagc tttttgcaaa agcctaggcc tccaaaaaag 3181 cctcctcact acttctggaa tagctcagag gccgaggcgg cctcggcctc tgcataaata 3241 aaaaaaatta gtcagccatg gggcggagaa tgggcggaac tgggcggagt taggggcggg 3301 atgggcggag ttaggggcgg gactatggtt gctgactaat tgagatgcat gctttgcata 3361 cttctgcctg ctggggagcc tggggacttt ccacacctgg ttgctgacta attgagatgc 3421 atgctttgca tacttctgcc tgctggggag cctggggact ttccacaccc taactgacac 3481 acattccaca gtcgggcagc gttgggtcct ggccacgggt gcgcatgatc gtgctcctgt 3541 cgttgaggac ccggctaggc tggcggggtt gccttactgg ttagcagaat gaatcaccga 3601 tacgcgagcg aacgtgaagc gactgctgct gcaaaacgtc tgcgacctga gcaacaacat 3661 gaatggtctt cggtttccgt gtttcgtaaa gtctggaaac gcggaagtca gcgctcttcc 3721 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct 3781 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg 3841 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc 3901 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga 3961 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct 4021 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg 4081 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag 4141 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat 4201 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac 4261 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac 4321 tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc 4381 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt 4441 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc 4501 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg 4561 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca 4621 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca 4681 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag 4741 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac 4801 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc 4861 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct 4921 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tgcaggcatc 4981 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg 5041 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc 5101 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat 5161 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag 5221 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aacacgggat 5281 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg 5341 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca 5401 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga 5461 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc 5521 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata 5581 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg 5641 ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc 5701 acgaggccct ttcgtcttca agaa //