LOCUS LT727610 4924 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian expression vector pSV23SE6Hf1, complete sequence.
ACCESSION LT727610
VERSION LT727610.1
KEYWORDS .
SOURCE Mammalian expression vector pSV23SE6Hf1
ORGANISM Mammalian expression vector pSV23SE6Hf1
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 4924)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..4924
/organism="Mammalian expression vector pSV23SE6Hf1"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 1980."
/db_xref="taxon:1945068"
CDS <1..809
/codon_start=3
/transl_table=11
/note="part of SV40 VP1"
/protein_id="SJL88969.1"
/translation="PLPNLNEDLTCGNILMWEAVTVKTEVIGVTAMLNLHSGTQKTHE
NGAGKPIQGSNFHFFAVGGEPLELQGVLANYRTKYPAQTVTPKNATVDSQQMNTDHKA
VLDKDNAYPVECWVPDPSKNENTRYFGTYTGGENVPPVLHITNTATTVLLDEQGVGPL
CKADSLYVSAVDICGLFTNTSGTQQWKGLPRYFKITLRKRSVKNPYPISFLLSDLINR
RTQRVDGQPMIGMSSQVEEVRVYEDTEELPGDPDMIRYIDEFGQTTTRMQ"
regulatory 810..906
/note="polyA"
/regulatory_class="terminator"
regulatory complement(810..906)
/note="polyA"
/regulatory_class="terminator"
misc_feature complement(907..1458)
/note="3' UTR of SV40 small t-antigen"
misc_feature complement(1459..1525)
/note="intron of SV40 small t-antigen"
CDS complement(1526..>1600)
/codon_start=1
/transl_table=11
/note="part of SV40 small t-antigen"
/protein_id="SJL88970.1"
/translation="DLCEGTLLLWCDIIGQTTYRDLKL"
CDS complement(1609..2331)
/transl_table=11
/note="mIghg2b"
/note="fragment of heavy chain of mouse anti-hPLAP
monoclonal antibody (E6H)"
/protein_id="SJL88971.1"
/translation="MEWIWIFLFILSGTAGVQSQVQLQQSGAELARPGASVKLSCKAS
GYTLTSYGISWVKQRTGQGLEWIGEIYPGSGNSYFNEKFKGKATLTVDKSSSTAYLHL
SSLTSEDSAVYFCAGPRQVGLLPFGYWGQGTLVTASAAKTTPPSVYPLAPGCGDTTGS
SVTLGCLVKGYFPESVTVTWNSGSLSSSVHTFPALLQSGLYTMSSSVTVPSSTWPSQT
VTCSVAHPASSTTVDKKLEPSV"
rep_origin complement(2378..2453)
/note="SV40 ori"
regulatory complement(2410..2691)
/note="SV40 early promoter"
/regulatory_class="promoter"
misc_feature 2692..4924
/note="pAT"
rep_origin complement(2910..3315)
/note="pMB1 ori"
CDS complement(3856..4716)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL88972.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
BASE COUNT 1309 a 1129 c 1192 g 1294 t
ORIGIN
1 ttcctttgcc taatttaaat gaggacttaa cctgtggaaa tattttgatg tgggaagctg
61 ttactgttaa aactgaggtt attggggtaa ctgctatgtt aaacttgcat tcagggacac
121 aaaaaactca tgaaaatggt gctggaaaac ccattcaagg gtcaaatttt catttttttg
181 ctgttggtgg ggaacctttg gagctgcagg gtgtgttagc aaactacagg accaaatatc
241 ctgctcaaac tgtaacccca aaaaatgcta cagttgacag tcagcagatg aacactgacc
301 acaaggctgt tttggataag gataatgctt atccagtgga gtgctgggtt cctgatccaa
361 gtaaaaatga aaacactaga tattttggaa cctacacagg tggggaaaat gtgcctcctg
421 ttttgcacat tactaacaca gcaaccacag tgcttcttga tgagcagggt gttgggccct
481 tgtgcaaagc tgacagcttg tatgtttctg ctgttgacat ttgtgggctg tttaccaaca
541 cttctggaac acagcagtgg aagggacttc ccagatattt taaaattacc cttagaaagc
601 ggtctgtgaa aaacccctac ccaatttcct ttttgttaag tgacctaatt aacaggagga
661 cacagagggt ggatgggcag cctatgattg gaatgtcctc tcaagtagag gaggttaggg
721 tttatgagga cacagaggag cttcctgggg atccagacat gataagatac attgatgagt
781 ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg
841 ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca
901 ttcattttat gtttcaggtt cagggggagg tgtgggaggt tttttaaagc aagtaaaacc
961 tctacaaatg tggtatggct gattatgatc tctagtcaag gcactataca tcaaatattc
1021 cttattaacc cctttacaaa ttaaaaagct aaaggtacac aatttttgag catagttatt
1081 aatagcagac actctatgcc tgtgtggagt aagaaaaaac agtatgttat gattataact
1141 gttatgccta cttataaagg ttacagaata tttttccata attttcttgt atagcagtgc
1201 agctttttcc tttgtggtgt aaatagcaaa gcaagcaaga gttctattac taaacacagc
1261 atgactcaaa aaacttagca attctgaagg aaagtccttg gggtcttcta cctttctctt
1321 cttttttgga ggagtagaat gttgagagtc agcagtagcc tcatcatcac tagatggcat
1381 ttcttctgag caaaacaggt tttcctcatt aaaggcattc caccactgct cccattcatc
1441 agttccatag gttggaatct aaaatacaca aacaattaga atcagtagtt taacacatta
1501 tacacttaaa aattttatat ttaccttaga gctttaaatc tctgtaggta gtttgtccaa
1561 ttatgtcaca ccacagaagt aaggttcctt cacaaagatc cgtcgactct agacgctggg
1621 ctcaagtttt ttgtccaccg tggtgctgct ggctgggtga gcaacactgc aggtaacggt
1681 ctggcttggc caggtgctgg aggggacagt cactgagctg ctcatagtgt agagtccaga
1741 ctgcaggaga gctgggaagg tgtgcacact gctggacagg gatccggagt tccaagtcac
1801 agtcactgac tcagggaagt agcccttgac caggcatccc agagtcacgg aggaaccagt
1861 tgtatctcca cacccagggg ccagtggata gactgatggg ggtgttgttt tggctgcaga
1921 ggcagtgacc agagtccctt ggccccagta accaaaaggg agtagcccga cctgtctagg
1981 tccggcacag aaatagacgg cagagtcctc agatgtcagg ctgctgaggt gcaagtaggc
2041 tgtgctggag gatttgtcta cagtcagtgt ggccttgccc ttaaacttct cattgaagta
2101 cgaatttcca cttccaggat aaatttctcc aatccactca aggccctgtc cagttctctg
2161 cttcacccaa cttataccat aacttgtgag ggtgtatcca gaggccttgc aggacagctt
2221 cactgaggcc ccaggcctcg ccagctcagc tccagactgc tgcagctgaa cctgggattg
2281 gacacctgca gttcctgaca ggatgaagag aaagatccag atccattcca tgctagagcc
2341 aagcttgagc tttttgcaaa agcctaggcc tccaaaaaag cctcctcact acttctggaa
2401 tagctcagag gccgaggcgg cctcggcctc tgcataaata aaaaaaatta gtcagccatg
2461 gggcggagaa tgggcggaac tgggcggagt taggggcggg atgggcggag ttaggggcgg
2521 gactatggtt gctgactaat tgagatgcat gctttgcata cttctgcctg ctggggagcc
2581 tggggacttt ccacacctgg ttgctgacta attgagatgc atgctttgca tacttctgcc
2641 tgctggggag cctggggact ttccacaccc taactgacac acattccaca gtcgggcagc
2701 gttgggtcct ggccacgggt gcgcatgatc gtgctcctgt cgttgaggac ccggctaggc
2761 tggcggggtt gccttactgg ttagcagaat gaatcaccga tacgcgagcg aacgtgaagc
2821 gactgctgct gcaaaacgtc tgcgacctga gcaacaacat gaatggtctt cggtttccgt
2881 gtttcgtaaa gtctggaaac gcggaagtca gcgctcttcc gcttcctcgc tcactgactc
2941 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
3001 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
3061 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
3121 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
3181 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
3241 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
3301 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
3361 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
3421 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
3481 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac
3541 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
3601 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
3661 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
3721 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
3781 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
3841 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
3901 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
3961 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
4021 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
4081 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
4141 taatagtttg cgcaacgttg ttgccattgc tgcaggcatc gtggtgtcac gctcgtcgtt
4201 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
4261 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
4321 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
4381 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
4441 gcggcgaccg agttgctctt gcccggcgtc aacacgggat aataccgcgc cacatagcag
4501 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
4561 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
4621 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
4681 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
4741 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
4801 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac
4861 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtcttca
4921 agaa
//