LOCUS LT727609 8952 bp DNA circular SYN 06-FEB-2017
DEFINITION Vector p220.2, complete sequence.
ACCESSION LT727609
VERSION LT727609.1
KEYWORDS .
SOURCE Vector p220.2
ORGANISM Vector p220.2
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 8952)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..8952
/organism="Vector p220.2"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 1966."
/db_xref="taxon:1945237"
CDS 418..2343
/transl_table=11
/note="EBNA1"
/protein_id="SJL88966.1"
/translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRG
RGRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGG
AGAGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAG
GGAGGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGA
GAGGGAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAG
GAGAGGGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGS
GGRGRGGSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSG
SPPRRPPPGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGP
RGQGDGGRRKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVF
VYGGSKTSLYNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFL
QTHIFAEVLKDAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDG
DDGDEGGDGDEGEEGQE"
rep_origin 2645..4416
/note="Epstein-Barr oriP"
regulatory complement(5372..5441)
/note="HSV-TK polyA"
/regulatory_class="terminator"
CDS complement(5442..6479)
/transl_table=11
/note="hygromycin resistance"
/protein_id="SJL88967.1"
/translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQD
LPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVY
HWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWS
EAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQ
SLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPS
TRGDREMGEAN"
regulatory complement(6554..6664)
/note="HSV-TK promoter"
/regulatory_class="promoter"
rep_origin complement(7079..7343)
/note="pMB1 ori"
CDS complement(7884..8744)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL88968.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
BASE COUNT 2191 a 2206 c 2632 g 1923 t
ORIGIN
1 ttctcatgtt tgacagctta tcatcgataa gctgatcctc acaggccgca cccagctttt
61 cttccgttgc cccagtagca tctctgtctg gtgaccttga agaggaagag gaggggtccc
121 gagaatcccc atccctaccg tccagcaaaa agggggacga ggaatttgag gcctggcttg
181 aggctcagga cgcaaatctt gaggatgttc agcgggagtt ttccgggctg cgagtaattg
241 gtgatgagga cgaggatggt tcggaggatg gggaattttc agacctggat ctgtctgaca
301 gcgaccatga aggggatgag ggtggggggg ctgttggagg gggcaggagt ctgcactccc
361 tgtattcact gagcgtcgtc taataaagat gtctattgat ctcttttagt gtgaatcatg
421 tctgacgagg ggccaggtac aggacctgga aatggcctag gagagaaggg agacacatct
481 ggaccagaag gctccggcgg cagtggacct caaagaagag ggggtgataa ccatggacga
541 ggacggggaa gaggacgagg acgaggaggc ggaagaccag gagccccggg cggctcagga
601 tcagggccaa gacatagaga tggtgtccgg agaccccaaa aacgtccaag ttgcattggc
661 tgcaaaggga cccacggtgg aacaggagca ggagcaggag cgggaggggc aggagcagga
721 ggggcaggag caggaggagg ggcaggagca ggaggagggg caggaggggc aggaggggca
781 ggaggggcag gagcaggagg aggggcagga gcaggaggag gggcaggagg ggcaggaggg
841 gcaggagcag gaggaggggc aggagcagga ggaggggcag gaggggcagg agcaggagga
901 ggggcaggag gggcaggagg ggcaggagca ggaggagggg caggagcagg aggaggggca
961 ggaggggcag gagcaggagg aggggcagga ggggcaggag gggcaggagc aggaggaggg
1021 gcaggagcag gaggggcagg aggggcagga ggggcaggag caggaggggc aggagcagga
1081 ggaggggcag gaggggcagg aggggcagga gcaggagggg caggagcagg aggggcagga
1141 gcaggagggg caggagcagg aggggcagga ggggcaggag caggaggggc aggaggggca
1201 ggagcaggag gggcaggagg ggcaggagca ggaggagggg caggaggggc aggagcagga
1261 ggaggggcag gaggggcagg agcaggaggg gcaggagggg caggagcagg aggggcagga
1321 ggggcaggag caggaggggc aggaggggca ggagcaggag gaggggcagg agcaggaggg
1381 gcaggagcag gaggtggagg ccggggtcga ggaggcagtg gaggccgggg tcgaggaggt
1441 agtggaggcc ggggtcgagg aggtagtgga ggccgccggg gtagaggacg tgaaagagcc
1501 agggggggaa gtcgtgaaag agccaggggg agaggtcgtg gacgtggaga aaagaggccc
1561 aggagtccca gtagtcagtc atcatcatcc gggtctccac cgcgcaggcc ccctccaggt
1621 agaaggccat ttttccaccc tgtaggggaa gccgattatt ttgaatacca ccaagaaggt
1681 ggcccagatg gtgagcctga cgtgcccccg ggagcgatag agcagggccc cgcagatgac
1741 ccaggagaag gcccaagcac tggaccccgg ggtcagggtg atggaggcag gcgcaaaaaa
1801 ggagggtggt ttggaaagca tcgtggtcaa ggaggttcca acccgaaatt tgagaacatt
1861 gcagaaggtt taagagctct cctggctagg agtcacgtag aaaggactac cgacgaagga
1921 acttgggtcg ccggtgtgtt cgtatatgga ggtagtaaga cctcccttta caacctaagg
1981 cgaggaactg cccttgctat tccacaatgt cgtcttacac cattgagtcg tctccccttt
2041 ggaatggccc ctggacccgg cccacaacct ggcccgctaa gggagtccat tgtctgttat
2101 ttcatggtct ttttacaaac tcatatattt gctgaggttt tgaaggatgc gattaaggac
2161 cttgttatga caaagcccgc tcctacctgc aatatcaggg tgactgtgtg cagctttgac
2221 gatggagtag atttgcctcc ctggtttcca cctatggtgg aaggggctgc cgcggagggt
2281 gatgacggag atgacggaga tgaaggaggt gatggagatg agggtgagga agggcaggag
2341 tgatgtaact tgttaggaga cgccctcaat cgtattaaaa gccgtgtatt cccccgcact
2401 aaagaataaa tccccagtag acatcatgcg tgctgttggt gtatttctgg ccatctgtct
2461 tgtcaccatt ttcgtcctcc caacatgggg caattgggca tacccatgtt gtcacgtcac
2521 tcagctccgc gctcaacacc ttctcgcgtt ggaaaacatt agcgacattt acctggtgag
2581 caatcagaca tgcgacggct ttagcctggc ctccttaaat tcacctaaga atgggagcaa
2641 ccagcaggaa aaggacaagc agcgaaaatt cacgccccct tgggaggtgg cggcatatgc
2701 aaaggatagc actcccactc tactactggg tatcatatgc tgactgtata tgcatgagga
2761 tagcatatgc tacccggata cagattagga tagcatatac tacccagata tagattagga
2821 tagcatatgc tacccagata tagattagga tagcctatgc tacccagata taaattagga
2881 tagcatatac tacccagata tagattagga tagcatatgc tacccagata tagattagga
2941 tagcctatgc tacccagata tagattagga tagcatatgc tacccagata tagattagga
3001 tagcatatgc tatccagata tttgggtagt atatgctacc cagatataaa ttaggatagc
3061 atatactacc ctaatctcta ttaggatagc atatgctacc cggatacaga ttaggatagc
3121 atatactacc cagatataga ttaggatagc atatgctacc cagatataga ttaggatagc
3181 ctatgctacc cagatataaa ttaggatagc atatactacc cagatataga ttaggatagc
3241 atatgctacc cagatataga ttaggatagc ctatgctacc cagatataga ttaggatagc
3301 atatgctatc cagatatttg ggtagtatat gctacccatg gcaacattag cccaccgtgc
3361 tctcagcgac ctcgtgaata tgaggaccaa caaccctgtg cttggcgctc aggcgcaagt
3421 gtgtgtaatt tgtcctccag atcgcagcaa tcgcgcccct atcttggccc gcccacctac
3481 ttatgcaggt attccccggg gtgccattag tggttttgtg ggcaagtggt ttgaccgcag
3541 tggttagcgg ggttacaatc agccaagtta ttacaccctt attttacagt ccaaaaccgc
3601 agggcggcgt gtgggggctg acgcgtgccc ccactccaca atttcaaaaa aaagagtggc
3661 cacttgtctt tgtttatggg ccccattggc gtggagcccc gtttaatttt cgggggtgtt
3721 agagacaacc agtggagtcc gctgctgtcg gcgtccactc tctttcccct tgttacaaat
3781 agagtgtaac aacatggttc acctgtcttg gtccctgcct gggacacatc ttaataaccc
3841 cagtatcata ttgcactagg attatgtgtt gcccatagcc ataaattcgt gtgagatgga
3901 catccagtct ttacggcttg tccccacccc atggatttct attgttaaag atattcagaa
3961 tgtttcattc ctacactagt atttattgcc caaggggttt gtgagggtta tattggtgtc
4021 atagcacaat gccaccactg aaccccccgt ccaaatttta ttctgggggc gtcacctgaa
4081 accttgtttt cgagcacctc acatacacct tactgttcac aactcagcag ttattctatt
4141 agctaaacga aggagaatga agaagcaggc gaagattcag gagagttcac tgcccgctcc
4201 ttgatcttca gccactgccc ttgtgactaa aatggttcac taccctcgtg gaatcctgac
4261 cccatgtaaa taaaaccgtg acagctcatg gggtgggaga tatcgctgtt ccttaggacc
4321 cttttactaa ccctaattcg atagcatatg cttcccgttg ggtaacatat gctattgaat
4381 tagggttagt ctggatagta tatactacta cccgggaagc atatgctacc cgtttagggt
4441 taacaagggg gccttataaa cactattgct aatgccctct tgagggtccg cttatcggta
4501 gctacacagg cccctctgat tgacgttggt gtagcctccc gtagtcttcc tgggcccctg
4561 ggaggtacat gtcccccagc attggtgtaa gagcttcagc caagagttac acataaaggc
4621 aatgttgtgt tgcagtccac agactgcaaa gtctgctcca ggatgaaagc cactcagtgt
4681 tggcaaatgt gcacatccat ttataaggat gtcaactaca gtcagagaac ccctttgtgt
4741 ttggtccccc cccgtgtcac atgtggaaca gggcccagtt ggcaagttgt accaaccaac
4801 tgaagggatt acatgcactg ccccgtgacc aatacaaaac aaaagcgctc ctcgtaccag
4861 cgaagaaggg gcagagatgc cgtagtcagg tttagttcgt ccggcggcgg gggatcctct
4921 agagtcgacc tgcagcccaa gcttggcact ggcgccagaa atccgcgcgg tggtttttgg
4981 gggtcggggg tgtttggcag ccacagacgc ccggtgttcg tgtcgcgcca gtacatgcgg
5041 tccatgccca ggccatccaa aaaccatggg tctgtctgct cagtccagtc gtggacctga
5101 ccccacgcaa cgcccaaaag aataaccccc acgaaccata aaccattccc catgggggac
5161 cccgtcccta acccacgggg cccgtggcta tggcgggctt gccgccccga cgttggctgc
5221 gagccctggg ccttcacccg aacttggggg ttggggtggg gaaaaggaag aaacgcgggc
5281 gtattggccc caatggggtc tcggtggggt atcgacagag tgccagccct gggaccgaac
5341 cccgcgttta tgaacaaacg acccaacacc cgtgcgtttt attctgtctt tttattgccg
5401 tcatagcgcg ggttccttcc ggtattgtct ccttccgtgt ttcagttagc ctcccccatc
5461 tcccgatccc cacgagtgct ggggcgtcgg tttccactat cggcgagtac ttctacacag
5521 ccatcggtcc agacggccgc gcttctgcgg gcgatttgtg tacgcccgac agtcccggct
5581 ccggatcgga cgattgcgtc gcatcgaccc tgcgcccaag ctgcatcatc gaaattgccg
5641 tcaaccaagc tctgatagag ttggtcaaga ccaatgcgga gcatatacgc ccggagccgc
5701 ggcgatcctg caagctccgg atgcctccgc tcgaagtagc gcgtctgctg ctccatacaa
5761 gccaaccacg gcctccagaa gaagatgttg gcgacctcgt attgggaatc cccgaacatc
5821 gcctcgctcc agtcaatgac cgctgttatg cggccattgt ccgtcaggac attgttggag
5881 ccgaaatccg cgtgcacgag gtgccggact tcggggcagt cctcggccca aagcatcagc
5941 tcatcgagag cctgcgcgac ggacgcactg acggtgtcgt ccatcacagt ttgccagtga
6001 tacacatggg gatcagcaat cgcgcatatg aaatcacgcc atgtagtgta ttgaccgatt
6061 ccttgcggtc cgaatgggcc gaacccgctc gtctggctaa gatcggccgc agcgatcgca
6121 tccatggcct ccgcgaccgg ctgcagaaca gcgggcagtt cggtttcagg caggtcttgc
6181 aacgtgacac cctgtgcacg gcgggagatg caataggtca ggctctcgct gaattcccca
6241 atgtcaagca cttccggaat cgggagcgcg gccgatgcaa agtgccgata aacataacga
6301 tctttgtaga aaccatcggc gcagctattt acccgcagga catatccacg ccctcctaca
6361 tcgaagctga aagcacgaga ttcttcgccc tccgagagct gcatcaggtc ggagacgctg
6421 tcgaactttt cgatcagaaa cttctcgaca gacgtcgcgg tgagttcagg ctttttcata
6481 tctcattgcc cccggggatc ttgcggcacg ctgttgacgc tgttaagcgg gtcgctgcag
6541 ggtcgctcgg tgttcgaggc cacacgcgtc accttaatat gcgaagtgga cctgggaccg
6601 cgccgccccg actgcatctg cgtgttcgaa ttcgccaatg acaagacgct gggcggggtt
6661 tgtgtcatca tagaactaaa gacatgcaaa tatatttctt ccggggacac cgccagcaaa
6721 cgcgagcaac gggccacggg gatgaagcag cccggcggca cctcgctaac ggattcacca
6781 ctccaagaat tggagccaat caattcttgc ggagaactgt gaatgcgcaa accaaccctt
6841 ggcagaacat atccatcgcg tccgccatct ccagcagccg cacgcggcgc atctcggggc
6901 cgacgcgctg ggctacgtct tgctggcgtt cgcgacgcga ggctggatgg ccttccccat
6961 tatgattctt ctcgcttccg gcggcatcgg gatgcccgcg ttgcaggcca tgctgtccag
7021 gcaggtagat gacgaccatc agggacagct tcaaggatcg ctcgcggctc ttaccagcgc
7081 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
7141 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
7201 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
7261 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
7321 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
7381 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
7441 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
7501 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
7561 agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
7621 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
7681 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
7741 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
7801 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
7861 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
7921 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
7981 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
8041 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
8101 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
8161 tcgccagtta atagtttgcg caacgttgtt gccattgctg caggcatcgt ggtgtcacgc
8221 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
8281 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
8341 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
8401 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
8461 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa taccgcgcca
8521 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
8581 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
8641 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
8701 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
8761 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
8821 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
8881 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt
8941 cgtcttcaag aa
//