LOCUS LT727608 6249 bp DNA circular SYN 06-FEB-2017
DEFINITION Vector pIVHA, complete sequence.
ACCESSION LT727608
VERSION LT727608.1
KEYWORDS .
SOURCE Vector pIVHA
ORGANISM Vector pIVHA
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 6249)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..6249
/organism="Vector pIVHA"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 1960."
/db_xref="taxon:1945322"
CDS 86..1276
/transl_table=11
/note="tetracyclin resistance"
/protein_id="SJL88964.1"
/translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASH
YGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAG
RIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLH
APFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTV
FFIMQLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGE
KQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQG
QLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSR
ATST"
rep_origin complement(2350..2752)
/note="pMB1 ori"
misc_feature 3682..3710
/note="5UTR"
misc_feature 3711..3758
/note="sIVHA"
CDS <3759..5414
/codon_start=1
/transl_table=11
/note="IVHA"
/protein_id="SJL88965.1"
/translation="QDLPGNDNNSTATLCLGHHAVPNGTLVKTITNDQIEVTNATELV
QSSSTGKICNNPHRILDGINCTLIDALLGDPHCDGFQNEKWDLFVERSKAFSNCYPYD
VPDYASLRSLVASSGTLEFINEGFNWTGVTQNGGSSACKRGPDSGFFSRLNWLYKSGS
TYPVQNVTMPNNDNSDKLYIWGVHHPSTDKEQTNLYVQASGKVTVSTKRSQQTIIPNV
GSRPWVRGLSSRISIYWTIVKPGDILVINSNGNLIAPRGYFKMRTGKSSIMRSDAPIG
TCSSECITPNGSIPNDKPFQNVNKITYGACPKYVKQNTLKLATGMRNVPEKQTRGIFG
AIAGFIENGWEGMIDGWYGFRHQNSEGTGQAADLKSTQAAIDQINGKLNRVIEKTNEK
FHQIEKEFSEVEGRIQDLEKYVEDTKIDLWSYNAELLVALENQHTIDLTDSEMNKLFE
KTRRQLRENAEDMGNGCFKIYHKCDNACIGSIRNGTYDHDVYRDEALNNRFQIKGVEL
KSGYKDWILWISFAISCFLLCVVLLGFIMWACQKGNIRCNICI"
misc_feature 5415..5425
/note="3UTR"
BASE COUNT 1623 a 1563 c 1546 g 1517 t
ORIGIN
1 ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa
61 ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg
121 caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt
181 gcgggatatc gtccattccg acagcatcgc cagtcactat ggcgtgctgc tagcgctata
241 tgcgttgatg caatttctat gcgcacccgt tctcggagca ctgtccgacc gctttggccg
301 ccgcccagtc ctgctcgctt cgctacttgg agccactatc gactacgcga tcatggcgac
361 cacacccgtc ctgtggatcc tctacgccgg acgcatcgtg gccggcatca ccggcgccac
421 aggtgcggtt gctggcgcct atatcgccga catcaccgat ggggaagatc gggctcgcca
481 cttcgggctc atgagcgctt gtttcggcgt gggtatggtg gcaggccccg tggccggggg
541 actgttgggc gccatctcct tgcatgcacc attccttgcg gcggcggtgc tcaacggcct
601 caacctacta ctgggctgct tcctaatgca ggagtcgcat aagggagagc gtcgaccgat
661 gcccttgaga gccttcaacc cagtcagctc cttccggtgg gcgcggggca tgactatcgt
721 cgccgcactt atgactgtct tctttatcat gcaactcgta ggacaggtgc cggcagcgct
781 ctgggtcatt ttcggcgagg accgctttcg ctggagcgcg acgatgatcg gcctgtcgct
841 tgcggtattc ggaatcttgc acgccctcgc tcaagccttc gtcactggtc ccgccaccaa
901 acgtttcggc gagaagcagg ccattatcgc cggcatggcg gccgacgcgc tgggctacgt
961 cttgctggcg ttcgcgacgc gaggctggat ggccttcccc attatgattc ttctcgcttc
1021 cggcggcatc gggatgcccg cgttgcaggc catgctgtcc aggcaggtag atgacgacca
1081 tcagggacag cttcaaggat cgctcgcggc tcttaccagc ctaacttcga tcactggacc
1141 gctgatcgtc acggcgattt atgccgcctc ggcgagcaca tggaacgggt tggcatggat
1201 tgtaggcgcc gccctatacc ttgtctgcct ccccgcgttg cgtcgcggtg catggagccg
1261 ggccacctcg acctgaatgg aagccggcgg cacctcgcta acggattcac cactccaaga
1321 attggagcca atcaattctt gcggagaact gtgaatgcgc aaaccaaccc ttggcagaac
1381 atatccatcg cgtccgccat ctccagcagc cgcacgcggc gcatctcggg cagcgttggg
1441 tcctggccac gggtgcgcat gatcgtgctc ctgtcgttga ggacccggct aggctggcgg
1501 ggttgcctta ctggttagca gaatgaatca ccgatacgcg agcgaacgtg aagcgactgc
1561 tgctgcaaaa cgtctgcgac ctgagcaaca acatgaatgg tcttcggttt ccgtgtttcg
1621 taaagtctgg aaacgcggaa gtcagcgccc tgcaccatta tgttccggat ctgcatcgca
1681 ggatgctgct ggctaccctg tggaacacct acatctgtat taacgaagcg ctggcattga
1741 ccctgagtga tttttctctg gtcccgccgc atccataccg ccagttgttt accctcacaa
1801 cgttccagta accgggcatg ttcatcatca gtaacccgta tcgtgagcat cctctctcgt
1861 ttcatcggta tcattacccc catgaacaga aatccccctt acacggaggc atcagtgacc
1921 aaacaggaaa aaaccgccct taacatggcc cgctttatca gaagccagac attaacgctt
1981 ctggagaaac tcaacgagct ggacgcggat gaacaggcag acatctgtga atcgcttcac
2041 gaccacgctg atgagcttta ccgcagctgc ctcgcgcgtt tcggtgatga cggtgaaaac
2101 ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc
2161 agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc agccatgacc
2221 cagtcacgta gcgatagcgg agtgtatact ggcttaacta tgcggcatca gagcagattg
2281 tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc
2341 gcatcaggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
2401 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
2461 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
2521 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
2581 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
2641 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
2701 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
2761 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
2821 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
2881 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
2941 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
3001 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
3061 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
3121 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
3181 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
3241 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
3301 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
3361 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
3421 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
3481 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
3541 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
3601 ccattgctgc aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
3661 aaaaaaaaaa aaaaaaaaaa aagcaaaagc aggggataat tctattaacc atgaagacta
3721 tcattgcttt gagctacatt ttctgtctgg ttttcgccca agaccttcca ggaaatgaca
3781 acaacagcac agcaacgctg tgcctgggac atcatgcggt gccaaacgga acgctagtga
3841 aaacaatcac gaatgatcag attgaagtga ctaatgctac tgagctggtt cagagttcct
3901 caacgggtaa aatatgcaac aatcctcatc gaatccttga tggaataaac tgcacactga
3961 tagatgctct attgggggac cctcattgtg atggatttca aaatgagaaa tgggaccttt
4021 tcgttgaacg cagcaaagct ttcagcaact gttaccctta tgatgtgcca gattatgcct
4081 cccttaggtc actagttgcc tcgtcaggca ctctggagtt tatcaatgaa ggcttcaatt
4141 ggactggggt cactcagaat gggggaagca gtgcttgcaa aagaggacct gatagcggtt
4201 ttttcagtag actgaactgg ttgtacaaat caggaagcac atatccagtg caaaacgtga
4261 ccatgccaaa caatgacaat tctgacaaac tatacatttg gggggttcac cacccgagca
4321 cggacaaaga acaaaccaac ctatatgttc aagcatcagg gaaagtcaca gtctccacca
4381 agagaagcca gcaaactata atcccgaatg tcgggtctag accctgggta aggggtctgt
4441 ctagtagaat aagcatctat tggacaatag ttaaaccggg agacatactg gtaattaata
4501 gtaatgggaa cctaattgct cctcggggtt acttcaaaat gcgcactggg aaaagctcaa
4561 taatgaggtc agatgcacct attggcacct gcagctctga atgcatcact ccaaatggaa
4621 gcattcccaa tgacaagccc tttcaaaacg taaacaagat cacatatggg gcatgtccca
4681 agtatgttaa gcaaaacact ctgaagttgg caacagggat gcggaatgta ccagagaaac
4741 aaactagagg catattcggc gcaatagcag gtttcataga aaatggttgg gagggaatga
4801 tagacggttg gtacggtttc aggcatcaaa attccgaggg cacaggacaa gcagcagatc
4861 ttaaaagcac tcaagcagcc atcgaccaaa tcaatgggaa actgaatagg gtaatcgaga
4921 agacgaacga gaaattccat caaatcgaaa aggaattctc agaagtagaa gggagaattc
4981 aggacctcga gaaatacgtt gaagacacta aaatagatct ctggtcttac aatgcggagc
5041 ttcttgtcgc tctggagaac caacatacaa ttgatctgac tgactcggaa atgaacaaac
5101 tgtttgaaaa aacaaggagg caactgaggg aaaatgctga ggacatgggc aatggttgct
5161 tcaaaatata ccacaaatgt gacaatgctt gcatagggtc aatcagaaat gggacttatg
5221 accatgatgt atacagagac gaagcattaa acaaccggtt tcagatcaaa ggtgttgaac
5281 tgaagtcagg atacaaagac tggatcctgt ggatttcctt tgccatatca tgctttttgc
5341 tttgtgttgt tttgctgggg ttcatcatgt gggcctgcca aaaaggcaac attaggtgca
5401 acatttgcat ttgagtgtat tagtattttt tttttttttt tttttttttt tttttttttt
5461 tttttttttt tttttttttt tttttttttt ttttttgcag gcatcgtggt gtcacgctcg
5521 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
5581 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
5641 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
5701 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
5761 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaacac gggataatac cgcgccacat
5821 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
5881 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
5941 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
6001 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
6061 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
6121 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa
6181 gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt
6241 cttcaagaa
//