LOCUS LT727605 6080 bp DNA circular SYN 06-FEB-2017
DEFINITION Fungal expression vector pFGA6, complete sequence.
ACCESSION LT727605
VERSION LT727605.1
KEYWORDS .
SOURCE Fungal expression vector pFGA6
ORGANISM Fungal expression vector pFGA6
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 6080)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..6080
/organism="Fungal expression vector pFGA6"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 1943."
/db_xref="taxon:1944731"
regulatory 457..1565
/note="A. niger glaA promoter"
/regulatory_class="promoter"
misc_feature 1566..1606
/note="5' UTR of A. niger glaA"
exon 1607..1820
/note="mutated exon 1 of A. niger glaA"
intron 1821..1895
/note="intron 1 of A. niger glaA"
exon 1896..2182
/note="exon 2 of A. niger glaA"
intron 2183..2237
/note="intron 2 of A. niger glaA"
exon 2238..2334
/note="exon 3 of A. niger glaA"
intron 2335..2395
/note="intron 3 of A. niger glaA"
exon 2396..3033
/note="exon 4 of A. niger glaA"
intron 3034..3091
/note="intron 4 of A. niger glaA"
exon 3092..3778
/note="exon 5 of A. niger glaA"
misc_feature 3779..3834
/note="3' UTR of A. niger glaA"
regulatory complement(3902..3985)
/note="lac promoter"
/regulatory_class="promoter"
rep_origin complement(4077..4479)
/note="pMB1 ori"
CDS complement(5020..5880)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL88960.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
BASE COUNT 1386 a 1636 c 1591 g 1467 t
ORIGIN
1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gcttggctgc aggtcgacta
421 cctggcctca tacttcttct atgacgctag ccgagagcag cttgaagaac tagtggccct
481 gtacccagac accaccacgt acgggtctcc gttcaggaca ggcgcggcca acaactggta
541 tccgcaattt aagcgattgg ccgccattct cggcgacttg gtcttcacca ttacccggcg
601 ggcattcctc tcgtatgcag aggaaatctc ccctgatctt ccgaactggt cgtacctggc
661 gacctatgac tatggcaccc cagttctggg gaccttccac ggaagtgacc tgctgcaggt
721 gttctatggg atcaagccaa actatgcagc tagttctagc cacacgtact atctgagctt
781 tgtgtatacg ctggatccga actccaaccg gggggagtac attgagtggc cgcagtggaa
841 ggaatcgcgg cagttgatga atttcggagc gaacgacgcc agtctcctta cggatgattt
901 ccgcaacggg acatatgagt tcatcctgca gaataccgcg gcgttccaca tctgatgcca
961 ttggcggagg ggtccggacg gtcaggaact tagccttatg agatgaatga tggacgtgtc
1021 tggcctcgga aaaggatata tggggatcat gatagtacta gccatattaa tgaagggcat
1081 ataccacgcg ttggacctgc gttatagctt cccgttagtt atagtaccat cgttatacca
1141 gccaatcaag tcaccacgca cgaccgggga cggcgaatcc ccgggaattg aaagaaattg
1201 catcccaggc cagtgaggcc agcgattggc cacctctcca aggcacaggg ccattctgca
1261 gcgctggtgg attcatcgca atttcccccg gcccggcccg acaccgctat aggctggttc
1321 tcccacacca tcggagattc gtcgcctaat gtctcgtccg ttcacaagct gaagagcttg
1381 aagtggcgag atgtctctgc aggaattcaa gctagatgct aagcgatatt gcatggcaat
1441 atgtgttgat gcatgtgctt cttccttcag cttcccctcg tgcagatgag gtttggctat
1501 aaattgaagt ggttggtcgg ggttccgtga ggggctgaag tgcttcctcc cttttagacg
1561 caactgagag cctgagcttc atccccagca tcattacacc tcagcaatgt cgttccgatc
1621 tctactcgcc ctgagcggcc tcgtctgcac agggttggca aatgtgattt ccaagaggcc
1681 taccttggat tcatggttga gcaacgaagc gaccgtggct cgtactgcca tcctgaataa
1741 catcggggcg gacggtgctt gggtgtcggg cgcggactct ggcattgtcg ttgctagtcc
1801 cagcacggat aacccggact gtatgtttcg agctcagatt tagtatgagt gtgtcattga
1861 ttgattgatg ctgactggcg tgtcgtttgt tgtagacttc tacacctgga ctcgcgactc
1921 tggtctcgtc ctcaagaccc tcgtcgatct cttccgaaat ggagatacca gtctcctctc
1981 caccattgag aactacatct ccgcccaggc aattgtccag ggtatcagta acccctctgg
2041 tgatctgtcc agcggcgctg gtctcggtga acccaagttc aatgtcgatg agactgccta
2101 cactggttct tggggacggc cgcagcgaga tggtccggct ctgagagcaa ctgctatgat
2161 cggcttcggg cagtggctgc ttgtatgttc tccaccccct tgcgtctgat ctgtgacata
2221 tgtagctgac tggtcaggac aatggctaca ccagcaccgc aacggacatt gtttggcccc
2281 tcgttaggaa cgacctgtcg tatgtggctc aatactggaa ccagacagga tatggtgtgt
2341 ttgttttatt ttaaatttcc aaagatgcgc cagcagagct aacccgcgat cgcagatctc
2401 tgggaagaag tcaatggctc gtctttcttt acgattgctg tgcaacaccg cgcccttgtc
2461 gaaggtagtg ccttcgcgac ggccgtcggc tcgtcctgct cctggtgtga ttctcaggca
2521 cccgaaattc tctgctacct gcagtccttc tggaccggca gcttcattct ggccaacttc
2581 gatagcagcc gttccggcaa ggacgcaaac accctcctgg gaagcatcca cacctttgat
2641 cctgaggccg catgcgacga ctccaccttc cagccctgct ccccgcgcgc gctcgccaac
2701 cacaaggagg ttgtagactc tttccgctca atctataccc tcaacgatgg tctcagtgac
2761 agcgaggctg ttgcggtggg tcggtaccct gaggacacgt actacaacgg caacccgtgg
2821 ttcctgtgca ccttggctgc cgcagagcag ttgtacgatg ctctatacca gtgggacaag
2881 caggggtcgt tggaggtcac agatgtgtcg ctggacttct tcaaggcact gtacagcgat
2941 gctgctactg gcacctactc ttcgtccagt tcgacttata gtagcattgt agatgccgtg
3001 aagactttcg ccgatggctt cgtctctatt gtggtaagtc tacgctagac aagcgctcat
3061 gttgacagag ggtgcgtact aacagaagta ggaaactcac gccgcaagca acggctccat
3121 gtccgagcaa tacgacaagt ctgatggcga gcagctttcc gctcgcgacc tgacctggtc
3181 ttatgctgct ctgctgaccg ccaacaaccg tcgtaactcc gtcgtgcctg cttcttgggg
3241 cgagacctct gccagcagcg tgcccggcac ctgtgcggcc acatctgcca ttggtaccta
3301 cagcagtgtg actgtcacct cgtggccgag tatcgtggct actggcggca ccactacgac
3361 ggctaccccc actggatccg gcagcgtgac ctcgaccagc aagaccaccg cgactgctag
3421 caagaccagc accagtacgt catcaacctc ctgtaccact cccaccgccg tggctgtgac
3481 tttcgatctg acagctacca ccacctacgg cgagaacatc tacctggtcg gatcgatctc
3541 tcagctgggt gactgggaaa ccagcgacgg catagctctg agtgctgaca agtacacttc
3601 cagcgacccg ctctggtatg tcactgtgac tctgccggct ggtgagtcgt ttgagtacaa
3661 gtttatccgc attgagagcg atgactccgt ggagtgggag agtgatccca accgagaata
3721 caccgttcct caggcgtgcg gaacgtcgac cgcgacggtg actgacacct ggcggtagac
3781 aatcaatcca tttcgctata gttaaaggat ggggatgagg gcaattggtt atatgatccc
3841 cgggaattcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
3901 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
3961 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
4021 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
4081 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
4141 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
4201 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
4261 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
4321 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
4381 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
4441 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
4501 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
4561 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
4621 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
4681 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac
4741 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
4801 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
4861 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
4921 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
4981 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
5041 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
5101 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
5161 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
5221 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
5281 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
5341 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
5401 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
5461 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
5521 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
5581 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
5641 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
5701 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
5761 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
5821 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
5881 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
5941 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
6001 agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg
6061 tatcacgagg ccctttcgtc
//