LOCUS LT727604 6080 bp DNA circular SYN 06-FEB-2017 DEFINITION Fungal expression vector pFGA5, complete sequence. ACCESSION LT727604 VERSION LT727604.1 KEYWORDS . SOURCE Fungal expression vector pFGA5 ORGANISM Fungal expression vector pFGA5 other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 6080) AUTHORS De Schamphelaire W. JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent, Technologiepark 927, 9052, BELGIUM. REFERENCE 2 AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K., Roggeman Fonseca M., Vanhoucke M., Beyaert R. TITLE BCCM/LMBP Plasmid collection JOURNAL Unpublished. FEATURES Location/Qualifiers source 1..6080 /organism="Fungal expression vector pFGA5" /mol_type="other DNA" /note="BCCM/LMBP Plasmid collection (Ghent University,Belgium) accession number LMBP 1942." /db_xref="taxon:1944730" regulatory 457..1565 /note="A. niger glaA promoter" /regulatory_class="promoter" misc_feature 1566..1606 /note="5' UTR of A. niger glaA" exon 1607..1820 /note="mutated exon 1 of A. niger glaA" intron 1821..1895 /note="intron 1 of A. niger glaA" exon 1896..2182 /note="exon 2 of A. niger glaA" intron 2183..2237 /note="intron 2 of A. niger glaA" exon 2238..2334 /note="exon 3 of A. niger glaA" intron 2335..2395 /note="intron 3 of A. niger glaA" exon 2396..3033 /note="exon 4 of A. niger glaA" intron 3034..3091 /note="intron 4 of A. niger glaA" exon 3092..3778 /note="exon 5 of A. niger glaA" misc_feature 3779..3834 /note="3' UTR of A. niger glaA" regulatory complement(3902..3985) /note="lac promoter" /regulatory_class="promoter" rep_origin complement(4077..4479) /note="pMB1 ori" CDS complement(5020..5880) /transl_table=11 /note="ampicillin resistance" /protein_id="SJL88959.1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" BASE COUNT 1382 a 1640 c 1594 g 1464 t ORIGIN 1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc 241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat 301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt 361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gcttggctgc aggtcgacta 421 cctggcctca tacttcttct atgacgctag ccgagagcag cttgaagaac tagtggccct 481 gtacccagac accaccacgt acgggtctcc gttcaggaca ggcgcggcca acaactggta 541 tccgcaattt aagcgattgg ccgccattct cggcgacttg gtcttcacca ttacccggcg 601 ggcattcctc tcgtatgcag aggaaatctc ccctgatctt ccgaactggt cgtacctggc 661 gacctatgac tatggcaccc cagttctggg gaccttccac ggaagtgacc tgctgcaggt 721 gttctatggg atcaagccaa actatgcagc tagttctagc cacacgtact atctgagctt 781 tgtgtatacg ctggatccga actccaaccg gggggagtac attgagtggc cgcagtggaa 841 ggaatcgcgg cagttgatga atttcggagc gaacgacgcc agtctcctta cggatgattt 901 ccgcaacggg acatatgagt tcatcctgca gaataccgcg gcgttccaca tctgatgcca 961 ttggcggagg ggtccggacg gtcaggaact tagccttatg agatgaatga tggacgtgtc 1021 tggcctcgga aaaggatata tggggatcat gatagtacta gccatattaa tgaagggcat 1081 ataccacgcg ttggacctgc gttatagctt cccgttagtt atagtaccat cgttatacca 1141 gccaatcaag tcaccacgca cgaccgggga cggcgaatcc ccgggaattg aaagaaattg 1201 catcccaggc cagtgaggcc agcgattggc cacctctcca aggcacaggg ccattctgca 1261 gcgctggtgg attcatcgca atttcccccg gcccggcccg acaccgctat aggctggttc 1321 tcccacacca tcggagattc gtcgcctaat gtctcgtccg ttcacaagct gaagagcttg 1381 aagtggcgag atgtctctgc aggaattcaa gctagatgct aagcgatatt gcatggcaat 1441 atgtgttgat gcatgtgctt cttccttcag cttcccctcg tgcagatgag gtttggctat 1501 aaattgaagt ggttggtcgg ggttccgtga ggggctgaag tgcttcctcc cttttagacg 1561 caactgagag cctgagcttc atccccagca tcattacacc tcagcaatgt cgttccgatc 1621 tctactcgcc ctgagcggcc tcgtctgcac agggttggcg ggcccgattt ccaagcgcgc 1681 gaccttggat tcatggttga gcaacgaagc gaccgtggct cgtactgcca tcctgaataa 1741 catcggggcg gacggtgctt gggtgtcggg cgcggactct ggcattgtcg ttgctagtcc 1801 cagcacggat aacccggact gtatgtttcg agctcagatt tagtatgagt gtgtcattga 1861 ttgattgatg ctgactggcg tgtcgtttgt tgtagacttc tacacctgga ctcgcgactc 1921 tggtctcgtc ctcaagaccc tcgtcgatct cttccgaaat ggagatacca gtctcctctc 1981 caccattgag aactacatct ccgcccaggc aattgtccag ggtatcagta acccctctgg 2041 tgatctgtcc agcggcgctg gtctcggtga acccaagttc aatgtcgatg agactgccta 2101 cactggttct tggggacggc cgcagcgaga tggtccggct ctgagagcaa ctgctatgat 2161 cggcttcggg cagtggctgc ttgtatgttc tccaccccct tgcgtctgat ctgtgacata 2221 tgtagctgac tggtcaggac aatggctaca ccagcaccgc aacggacatt gtttggcccc 2281 tcgttaggaa cgacctgtcg tatgtggctc aatactggaa ccagacagga tatggtgtgt 2341 ttgttttatt ttaaatttcc aaagatgcgc cagcagagct aacccgcgat cgcagatctc 2401 tgggaagaag tcaatggctc gtctttcttt acgattgctg tgcaacaccg cgcccttgtc 2461 gaaggtagtg ccttcgcgac ggccgtcggc tcgtcctgct cctggtgtga ttctcaggca 2521 cccgaaattc tctgctacct gcagtccttc tggaccggca gcttcattct ggccaacttc 2581 gatagcagcc gttccggcaa ggacgcaaac accctcctgg gaagcatcca cacctttgat 2641 cctgaggccg catgcgacga ctccaccttc cagccctgct ccccgcgcgc gctcgccaac 2701 cacaaggagg ttgtagactc tttccgctca atctataccc tcaacgatgg tctcagtgac 2761 agcgaggctg ttgcggtggg tcggtaccct gaggacacgt actacaacgg caacccgtgg 2821 ttcctgtgca ccttggctgc cgcagagcag ttgtacgatg ctctatacca gtgggacaag 2881 caggggtcgt tggaggtcac agatgtgtcg ctggacttct tcaaggcact gtacagcgat 2941 gctgctactg gcacctactc ttcgtccagt tcgacttata gtagcattgt agatgccgtg 3001 aagactttcg ccgatggctt cgtctctatt gtggtaagtc tacgctagac aagcgctcat 3061 gttgacagag ggtgcgtact aacagaagta ggaaactcac gccgcaagca acggctccat 3121 gtccgagcaa tacgacaagt ctgatggcga gcagctttcc gctcgcgacc tgacctggtc 3181 ttatgctgct ctgctgaccg ccaacaaccg tcgtaactcc gtcgtgcctg cttcttgggg 3241 cgagacctct gccagcagcg tgcccggcac ctgtgcggcc acatctgcca ttggtaccta 3301 cagcagtgtg actgtcacct cgtggccgag tatcgtggct actggcggca ccactacgac 3361 ggctaccccc actggatccg gcagcgtgac ctcgaccagc aagaccaccg cgactgctag 3421 caagaccagc accagtacgt catcaacctc ctgtaccact cccaccgccg tggctgtgac 3481 tttcgatctg acagctacca ccacctacgg cgagaacatc tacctggtcg gatcgatctc 3541 tcagctgggt gactgggaaa ccagcgacgg catagctctg agtgctgaca agtacacttc 3601 cagcgacccg ctctggtatg tcactgtgac tctgccggct ggtgagtcgt ttgagtacaa 3661 gtttatccgc attgagagcg atgactccgt ggagtgggag agtgatccca accgagaata 3721 caccgttcct caggcgtgcg gaacgtcgac cgcgacggtg actgacacct ggcggtagac 3781 aatcaatcca tttcgctata gttaaaggat ggggatgagg gcaattggtt atatgatccc 3841 cgggaattcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat 3901 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag 3961 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg 4021 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc 4081 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc 4141 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa 4201 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt 4261 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg 4321 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg 4381 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag 4441 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc 4501 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa 4561 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg 4621 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc 4681 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac 4741 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg 4801 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt 4861 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt 4921 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa 4981 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga 5041 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt 5101 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg 5161 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga 5221 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga 5281 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg 5341 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc 5401 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc 5461 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca 5521 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac 5581 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg 5641 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc 5701 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg 5761 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac 5821 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat 5881 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata 5941 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa 6001 agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg 6061 tatcacgagg ccctttcgtc //