LOCUS LT727598 5360 bp DNA circular SYN 06-FEB-2017
DEFINITION Vector pBRcHIL6f1, complete sequence.
ACCESSION LT727598
VERSION LT727598.1
KEYWORDS .
SOURCE Vector pBRcHIL6f1
ORGANISM Vector pBRcHIL6f1
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 5360)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..5360
/organism="Vector pBRcHIL6f1"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 1913."
/db_xref="taxon:1945259"
CDS 86..1276
/transl_table=11
/note="tetracyclin resistance"
/protein_id="SJL88946.1"
/translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASH
YGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAG
RIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLH
APFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTV
FFIMQLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGE
KQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQG
QLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSR
ATST"
rep_origin complement(2345..2755)
/note="pMB1 ori"
CDS <3699..4000
/codon_start=3
/transl_table=11
/note="hIL6f"
/protein_id="SJL88947.1"
/translation="KIITGLLEFEVYLEYLQNRFESSEEQARAVQMSTKVLIQFLQKK
AKNLDAITTPDPTTNASLLTKLQAQNQWLQDMTTHLILRSFKEFLQSSLRALRQM"
misc_feature 4001..4424
/note="3UTR"
BASE COUNT 1433 a 1336 c 1277 g 1314 t
ORIGIN
1 ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa
61 ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg
121 caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt
181 gcgggatatc gtccattccg acagcatcgc cagtcactat ggcgtgctgc tagcgctata
241 tgcgttgatg caatttctat gcgcacccgt tctcggagca ctgtccgacc gctttggccg
301 ccgcccagtc ctgctcgctt cgctacttgg agccactatc gactacgcga tcatggcgac
361 cacacccgtc ctgtggatcc tctacgccgg acgcatcgtg gccggcatca ccggcgccac
421 aggtgcggtt gctggcgcct atatcgccga catcaccgat ggggaagatc gggctcgcca
481 cttcgggctc atgagcgctt gtttcggcgt gggtatggtg gcaggccccg tggccggggg
541 actgttgggc gccatctcct tgcatgcacc attccttgcg gcggcggtgc tcaacggcct
601 caacctacta ctgggctgct tcctaatgca ggagtcgcat aagggagagc gtcgaccgat
661 gcccttgaga gccttcaacc cagtcagctc cttccggtgg gcgcggggca tgactatcgt
721 cgccgcactt atgactgtct tctttatcat gcaactcgta ggacaggtgc cggcagcgct
781 ctgggtcatt ttcggcgagg accgctttcg ctggagcgcg acgatgatcg gcctgtcgct
841 tgcggtattc ggaatcttgc acgccctcgc tcaagccttc gtcactggtc ccgccaccaa
901 acgtttcggc gagaagcagg ccattatcgc cggcatggcg gccgacgcgc tgggctacgt
961 cttgctggcg ttcgcgacgc gaggctggat ggccttcccc attatgattc ttctcgcttc
1021 cggcggcatc gggatgcccg cgttgcaggc catgctgtcc aggcaggtag atgacgacca
1081 tcagggacag cttcaaggat cgctcgcggc tcttaccagc ctaacttcga tcactggacc
1141 gctgatcgtc acggcgattt atgccgcctc ggcgagcaca tggaacgggt tggcatggat
1201 tgtaggcgcc gccctatacc ttgtctgcct ccccgcgttg cgtcgcggtg catggagccg
1261 ggccacctcg acctgaatgg aagccggcgg cacctcgcta acggattcac cactccaaga
1321 attggagcca atcaattctt gcggagaact gtgaatgcgc aaaccaaccc ttggcagaac
1381 atatccatcg cgtccgccat ctccagcagc cgcacgcggc gcatctcggg cagcgttggg
1441 tcctggccac gggtgcgcat gatcgtgctc ctgtcgttga ggacccggct aggctggcgg
1501 ggttgcctta ctggttagca gaatgaatca ccgatacgcg agcgaacgtg aagcgactgc
1561 tgctgcaaaa cgtctgcgac ctgagcaaca acatgaatgg tcttcggttt ccgtgtttcg
1621 taaagtctgg aaacgcggaa gtcagcgccc tgcaccatta tgttccggat ctgcatcgca
1681 ggatgctgct ggctaccctg tggaacacct acatctgtat taacgaagcg ctggcattga
1741 ccctgagtga tttttctctg gtcccgccgc atccataccg ccagttgttt accctcacaa
1801 cgttccagta accgggcatg ttcatcatca gtaacccgta tcgtgagcat cctctctcgt
1861 ttcatcggta tcattacccc catgaacaga aatccccctt acacggaggc atcagtgacc
1921 aaacaggaaa aaaccgccct taacatggcc cgctttatca gaagccagac attaacgctt
1981 ctggagaaac tcaacgagct ggacgcggat gaacaggcag acatctgtga atcgcttcac
2041 gaccacgctg atgagcttta ccgcagctgc ctcgcgcgtt tcggtgatga cggtgaaaac
2101 ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc
2161 agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc agccatgacc
2221 cagtcacgta gcgatagcgg agtgtatact ggcttaacta tgcggcatca gagcagattg
2281 tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc
2341 gcatcaggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
2401 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
2461 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
2521 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
2581 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
2641 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
2701 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
2761 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
2821 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
2881 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
2941 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
3001 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
3061 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
3121 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
3181 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
3241 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
3301 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
3361 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
3421 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
3481 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
3541 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
3601 ccattgctgc aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
3661 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaatg aaaatcatca ctggtctttt
3721 ggagtttgag gtatacctag agtacctcca gaacagattt gagagtagtg aggaacaagc
3781 cagagctgtg cagatgagta caaaagtcct gatccagttc ctgcagaaaa aggcaaagaa
3841 tctagatgca ataaccaccc ctgacccaac cacaaatgcc agcctgctga cgaagctgca
3901 ggcacagaac cagtggctgc aggacatgac aactcatctc attctgcgca gctttaagga
3961 gttcctgcag tccagcctga gggctcttcg gcaaatgtag catgggcacc tcagattgtt
4021 gttgttaatg ggcattcctt cttctggtca gaaacctgtc cactgggcac agaacttatg
4081 ttgttctcta tggagaacta aaagtatgag cgttaggaca ctattttaat tatttttaat
4141 ttattaatat ttaaatatgt gaagctgagt taatttatgt aagtcatatt tatattttta
4201 agaagtacca cttgaaacat tttatgtatt agttttgaaa taataatgga aagtggctat
4261 gcagtttgaa tatcctttgt ttcagagcca gatcatttct tggaaagtgt aggcttacct
4321 caaataaatg gctaacttat acatattttt aaagaaatat ttatattgta tttatataat
4381 gtataaatgg tttttatacc aataaatggc attttaaaaa attcaaaaaa aaaaaaaaaa
4441 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
4501 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaatttt
4561 tttttttttt tttttttttt tttttttttt tttttttttt tttttttgca ggcatcgtgg
4621 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
4681 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
4741 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
4801 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
4861 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaaca cgggataata
4921 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
4981 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
5041 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
5101 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
5161 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
5221 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
5281 ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga
5341 ggccctttcg tcttcaagaa
//