LOCUS LT727597 4431 bp DNA circular SYN 06-FEB-2017
DEFINITION Vector pLK15, complete sequence.
ACCESSION LT727597
VERSION LT727597.1
KEYWORDS .
SOURCE Vector pLK15
ORGANISM Vector pLK15
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 4431)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..4431
/organism="Vector pLK15"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 1912."
/db_xref="taxon:1945324"
regulatory complement(117..247)
/note="PL"
/regulatory_class="promoter"
CDS 615..1430
/transl_table=11
/note="kanamycin resistance"
/protein_id="SJL88944.1"
/translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLY
GKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIP
GKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGL
VDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVG
RVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
rep_origin complement(1710..2110)
/note="pMB1 ori"
CDS complement(<3085..4356)
/transl_table=11
/note="fd-3"
/protein_id="SJL88945.1"
/translation="MKKLLFAIPLVVPFYSHSAETVESCLAKPHTENSFTNVWKDDKT
LDRYANYEGCLWNATGVVVCTGDETQCYGTWVPIGLAIPENEGGGSEGGGSEGGGSEG
GGTKPPEYGDTPIPGYTYINPLDGTYPPGTEQNPANPNPSLEESQPLNTFMFQNNRFR
NRQGALTVYTGTVTQGTDPVKTYYQYTPVSSKAMYDAYWNGKFRDCAFHSGFNEDPFV
CEYQGQSSDLPQPPVNAGGGSGGGSGGGSEGGGSEGGGSEGGGSEGGGSGGGSGSGDF
DYEKMANANKGAMTENADENALQSDAKGKLDSVATDYGAAIDGFIGDVSGLANGNGAT
GDFAGSNSQMAQVGDGDNSPLMNNFRQYLPSLPQSVECRPYVFGAGKPYEFSIDCDKI
NLFRGVFAFLLYVATFMYVFSTFANILRNKES"
BASE COUNT 1222 a 1052 c 1028 g 1129 t
ORIGIN
1 ttccaatgct tcgtttcgta tcacacaccc caaagccttc tgctttgaat gctgcccttc
61 ttcagggctt aattttaaga gcgtcacctt catggtggtc agtgcgtcct gctgatgtgc
121 tcagtatcac cgccagtggt atttatgtca acaccgccag agataattta tcaccgcaga
181 tggttatctg tatgtttttt atatgaattt attttttgca ggggggcatt gtttggtagg
241 tgagagatcc tctacgccgg acgcatcgtg gccggcatca ccggcgctga ggtctgcctc
301 gtgaagaagg tgttgctgac tcataccagg cctgaatcgc cccatcatcc agccagaaag
361 tgagggagcc acggttgatg agagctttgt tgtaggtgga ccagttggtg attttgaact
421 tttgctttgc cacggaacgg tctgcgttgt cgggaagatg cgtgatctga tccttcaact
481 cagcaaaagt tcgatttatt caacaaagcc acgttgtgtc tcaaaatctc tgatgttaca
541 ttgcacaaga taaaaatata tcatcatgaa caataaaact gtctgcttac ataaacagta
601 atacaagggg tgttatgagc catattcaac gggaaacgtc ttgctcgagg ccgcgattaa
661 attccaacat ggatgctgat ttatatgggt ataaatgggc tcgcgataat gtcgggcaat
721 caggtgcgac aatctatcga ttgtatggga agcccgatgc gccagagttg tttctgaaac
781 atggcaaagg tagcgttgcc aatgatgtta cagatgagat ggtcagacta aactggctga
841 cggaatttat gcctcttccg accatcaagc attttatccg tactcctgat gatgcatggt
901 tactcaccac tgcgatcccc gggaaaacag cattccaggt attagaagaa tatcctgatt
961 caggtgaaaa tattgttgat gcgctggcag tgttcctgcg ccggttgcat tcgattcctg
1021 tttgtaattg tccttttaac agcgatcgcg tatttcgtct cgctcaggcg caatcacgaa
1081 tgaataacgg tttggttgat gcgagtgatt ttgatgacga gcgtaatggc tggcctgttg
1141 aacaagtctg gaaagaaatg cataagcttt tgccattctc accggattca gtcgtcactc
1201 atggtgattt ctcacttgat aaccttattt ttgacgaggg gaaattaata ggttgtattg
1261 atgttggacg agtcggaatc gcagaccgat accaggatct tgccatccta tggaactgcc
1321 tcggtgagtt ttctccttca ttacagaaac ggctttttca aaaatatggt attgataatc
1381 ctgatatgaa taaattgcag tttcatttga tgctcgatga gtttttctaa tcagaattgg
1441 ttaattggtt gtaacactgg cagagcatta cgctgacttg acgggacggc ggctttgttg
1501 aataaatcga acttttgctg agttgaagga tcagatcacg catcttcccg acaacgcaga
1561 ccgttccgtg gcaaagcaaa agttcaaaat caccaactgg tccacctaca acaaagctct
1621 catcaaccgt ggctccctca ctttctggct ggatgatggg gcgattcagg cctggtatga
1681 gtcagcaaca ccttcttcac gaggcagacc tcagcgcttt tccgcttcct cgctcactga
1741 ctcgctacgc tcggtcgttc gactgcggcg agcggtactg actcacacaa aaacggtaac
1801 acagttatcc acagaatcag gggataaggc cggaaagaac atgtgagcaa aagaccagga
1861 acaggaagaa ggccacgtag caggcgtttt tccataggct ccgcccccct gacgagcatc
1921 acaaaaatag acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
1981 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
2041 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
2101 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
2161 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
2221 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
2281 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg
2341 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
2401 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
2461 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
2521 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
2581 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
2641 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
2701 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
2761 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
2821 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
2881 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
2941 tgcgcaacgt tgttgccatt gctgcaggtc ctatcttacc gaagcccttt ttaaggaaag
3001 taagcagata gccgaacaaa gttaccagaa ggaaaccgag gaaacgcaat aataacggaa
3061 tacccaaaag aactggcatg attaagactc cttattacgc agtatgttag caaacgtcga
3121 aaatacatac ataaaggtgg caacatataa aagaaacgca aagacaccac ggaataagtt
3181 tattttgtca caatcaatag aaaattcata tggtttacca gcgccaaaga cataagggcg
3241 acattcaacc gactgaggca aagaaggtaa atattgacgg aaattattca ttaaaggtga
3301 attatcaccg tcaccgactt gagccatttg ggaattagag ccagcaaaat caccagtagc
3361 accattacca ttagcaaggc cggaaacgtc accaatgaaa ccatcgatag cagcaccgta
3421 atcagtagcg acagaatcaa gtttgccttt agcgtcagac tgtagcgcgt tttcatcggc
3481 attttcggtc atagccccct tattagcgtt tgccattttt tcataatcaa aatcaccgga
3541 accggagccg ccaccggaac cgccaccctc agagccgcca ccctcagaac cgccaccctc
3601 agagccgcca ccctcagagc cgccaccaga accaccacca gagccgccgc cagcattgac
3661 aggaggttga ggcaggtcag acgattggcc ttgatattca caaacgaatg gatcctcatt
3721 aaagccagaa tggaaagcgc agtctctgaa tttaccgttc cagtaagcgt catacatggc
3781 ttttgatgat acaggagtgt actggtaata agttttaacg gggtcagtgc cttgagtaac
3841 agtgcccgta taaacagtta atgcaccctg cctatttcgg aacctattat tctgaaacat
3901 gaaagtatta agaggctgag actcctcaag agaaggatta ggattagcgg ggttttgctc
3961 agtaccaggc ggataagtgc cgtcgagagg gttgatataa gtatagcccg gaataggtgt
4021 atcaccgtac tcaggaggtt tagtaccgcc accctcagaa ccgccaccct cagaaccgcc
4081 accctcagag ccaccaccct cattttcagg gatagcaagc ccaataggaa cccatgtacc
4141 gtaacactga gtttcgtcac cagtacaaac cacaacgcct gtagcattcc acagacagcc
4201 ctcatagtta gcgtaacgat ctaaagtttt gtcgtctttc cagacgttag taaatgaatt
4261 ttctgtatga ggttttgcta aacaactttc aacagtttca gcggagtgag aatagaaagg
4321 aacaactaaa ggaattgcga ataataattt tttcacgttg aaaatctcca aaaaaaaagg
4381 ctccaaaagg agcctttaat tgtatcggtt tatcaggacg gatccgggga a
//