LOCUS LT727592 9192 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian expression vector pSV51, complete sequence.
ACCESSION LT727592
VERSION LT727592.1
KEYWORDS .
SOURCE Mammalian expression vector pSV51
ORGANISM Mammalian expression vector pSV51
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 9192)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..9192
/organism="Mammalian expression vector pSV51"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 1829."
/db_xref="taxon:1945099"
regulatory 101..382
/note="SV40 early promoter"
/regulatory_class="promoter"
rep_origin 339..414
/note="SV40 ori"
CDS 453..977
/transl_table=11
/note="SV40 small t-antigen"
/protein_id="SJL88931.1"
/translation="MDKVLNREESLQLMDLLGLERSAWGNIPLMRKAYLKKCKEFHPD
KGGDEEKMKKMNTLYKKMEDGVKYAHQPDFGGFWDATEVFASSLNPGVDAMYCKQWPE
CAKKMSANCICLLCLLRMKHENRKLYRKDPLVWVDCYCFDCFRMWFGLDLCEGTLLLW
CDIIGQTTYRDLKL"
exon 453..698
/note="1st exon of SV40 large T-antigen"
intron 699..1044
/note="intron of SV40 large T-antigen"
intron 978..1044
/note="intron of SV40 small t-antigen"
misc_feature 1045..2925
/note="3' UTR of SV40 small t-antigen"
exon 1045..2925
/note="2nd exon of SV40 large T-antigen"
regulatory 2926..3022
/note="SV40 polyA early"
/regulatory_class="terminator"
regulatory complement(2926..3022)
/note="SV40 polyA late"
/regulatory_class="terminator"
regulatory complement(3218..3299)
/note="fd terminator"
/regulatory_class="terminator"
regulatory complement(3508..3589)
/note="fd terminator"
/regulatory_class="terminator"
misc_feature complement(3699..4636)
/note="major intron of 16S mRNA of SV40 VP1"
misc_feature complement(4637..4839)
/note="5' UTR of the SV40 late region"
regulatory complement(4840..5135)
/note="SV40 late promoter"
/regulatory_class="promoter"
regulatory 4891..5172
/note="SV40 early promoter"
/regulatory_class="promoter"
rep_origin 5129..5204
/note="SV40 ori"
CDS 5243..5767
/transl_table=11
/note="SV40 small t-antigen"
/protein_id="SJL88932.1"
/translation="MDKVLNREESLQLMDLLGLERSAWGNIPLMRKAYLKKCKEFHPD
KGGDEEKMKKMNTLYKKMEDGVKYAHQPDFGGFWDATEVFASSLNPGVDAMYCKQWPE
CAKKMSANCICLLCLLRMKHENRKLYRKDPLVWVDCYCFDCFRMWFGLDLCEGTLLLW
CDIIGQTTYRDLKL"
exon 5243..5488
/note="1st exon of SV40 large T-antigen"
intron 5489..5834
/note="intron of SV40 large T-antigen"
intron 5768..5834
/note="intron of SV40 small t-antigen"
misc_feature 5835..6897
/note="3' UTR of SV40 small t-antigen"
exon 5835..6897
/note="part of 2nd exon of SV40 large T-antigen"
rep_origin complement(7181..7583)
/note="pMB1 ori"
CDS complement(8124..8984)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL88933.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
BASE COUNT 2732 a 1856 c 2077 g 2527 t
ORIGIN
1 ttctcatgtt tgacagctta tcatcggcgc agcaccatgg cctgaaataa cctctgaaag
61 aggaacttgg ttaggtacct tctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt
121 agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
181 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
241 catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
301 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
361 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
421 aggcctaggc ttttgcaaaa agctttgcaa agatggataa agttttaaac agagaggaat
481 ctttgcagct aatggacctt ctaggtcttg aaaggagtgc ctgggggaat attcctctga
541 tgagaaaggc atatttaaaa aaatgcaagg agtttcatcc tgataaagga ggagatgaag
601 aaaaaatgaa gaaaatgaat actctgtaca agaaaatgga agatggagta aaatatgctc
661 atcaacctga ctttggaggc ttctgggatg caactgaggt atttgcttct tccttaaatc
721 ctggtgttga tgcaatgtac tgcaaacaat ggcctgagtg tgcaaagaaa atgtctgcta
781 actgcatatg cttgctgtgc ttactgagga tgaagcatga aaatagaaaa ttatacagga
841 aagatccact tgtgtgggtt gattgctact gcttcgattg ctttagaatg tggtttggac
901 ttgatctttg tgaaggaacc ttacttctgt ggtgtgacat aattggacaa actacctaca
961 gagatttaaa gctctaaggt aaatataaaa tttttaagtg tataatgtgt taaactactg
1021 attctaattg tttgtgtatt ttagattcca acctatggaa ctgatgaatg ggagcagtgg
1081 tggaatgcct ttaatgagga aaacctgttt tgctcagaag aaatgccatc tagtgatgat
1141 gaggctactg ctgactctca acattctact cctccaaaaa agaagagaaa ggtagaagac
1201 cccaaggact ttccttcaga attgctaagt tttttgagtc atgctgtgtt tagtaataga
1261 actcttgctt gctttgctat ttacaccaca aaggaaaaag ctgcactgct atacaagaaa
1321 attatggaaa aatattctgt aacctttata agtaggcata acagttataa tcataacata
1381 ctgttttttc ttactccaca caggcataga gtgtctgcta ttaataacta tgctcaaaaa
1441 ttgtgtacct ttagcttttt aatttgtaaa ggggttaata aggaatattt gatgtatagt
1501 gccttgacta gagatccatt ttctgttatt gaggaaagtt tgccaggtgg gttaaaggag
1561 catgatttta atccagaaga agcagaggaa actaaacaag tgtcctggaa gcttgtaaca
1621 gagtatgcaa tggaaacaaa atgtgatgat gtgttgttat tgcttgggat gtacttggaa
1681 tttcagtaca gttttgaaat gtgtttaaaa tgtattaaaa aagaacagcc cagccactat
1741 aagtaccatg aaaagcatta tgcaaatgct gctatatttg ctgacagcaa aaaccaaaaa
1801 accatatgcc aacaggctgt tgatactgtt ttagctaaaa agcgggttga tagcctacaa
1861 ttaactagag aacaaatgtt aacaaacaga tttaatgatc ttttggatag gatggatata
1921 atgtttggtt ctacaggctc tgctgacata gaagaatgga tggctggagt tgcttggcta
1981 cactgtttgt tgcccaaaat ggattcagtg gtgtatgact ttttaaaatg catggtgtac
2041 aacattccta aaaaaagata ctggctgttt aaaggaccaa ttgatagtgg taaaactaca
2101 ttagcagctg ctttgcttga attatgtggg gggaaagctt taaatgttaa tttgcccttg
2161 gacaggctga actttgagct aggagtagct attgaccagt ttttagtagt ttttgaggat
2221 gtaaagggca ctggagggga gtccagagat ttgccttcag gtcagggaat taataacctg
2281 gacaatttaa gggattattt ggatggcagt gttaaggtaa acttagaaaa gaaacaccta
2341 aataaaagaa ctcaaatatt tccccctgga atagtcacca tgaatgagta cagtgtgcct
2401 aaaacactgc aggccagatt tgtaaaacaa atagatttta ggcccaaaga ttatttaaag
2461 cattgcctgg aacgcagtga gtttttgtta gaaaagagaa taattcaaag tggcattgct
2521 ttgcttctta tgttaatttg gtacagacct gtggctgagt ttgctcaaag tattcagagc
2581 agaattgtgg agtggaaaga gagattggac aaagagttta gtttgtcagt gtatcaaaaa
2641 atgaagttta atgtggctat gggaattgga gttttagatt ggctaagaaa cagtgatgat
2701 gatgatgaag acagccagga aaatgctgat aaaaatgaag atggtgggga gaagaacatg
2761 gaagactcag ggcatgaaac aggcattgat tcacagtccc aaggctcatt tcaggcccct
2821 cagtcctcac agtctgttca tgatcataat cagccatacc acatttgtag aggttttact
2881 tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt
2941 tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
3001 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
3061 tgtatcttat catgtctgga tccggctcta gagggtcgac cctctagagg tcgaaattct
3121 gtgtgaaatt gttatccgct cagaattttc tgtatgaggt tttgctaaac aactttcaac
3181 agtttcagcg gagtgagaat agaaaggaac aactaaagga attgcgaata ataatttttt
3241 cacgttgaaa atctccaaaa aaaaaggctc caaaaggagc ctttaattgt atcggtttat
3301 cagcttgctt tcgaggtgaa tttcgaccct ctagagggtc gaccctctag aggctgcagc
3361 cgaccccgga tccggctcta gagggtcgac cctctagagg tcgaaattct gtgtgaaatt
3421 gttatccgct cagaattttc tgtatgaggt tttgctaaac aactttcaac agtttcagcg
3481 gagtgagaat agaaaggaac aactaaagga attgcgaata ataatttttt cacgttgaaa
3541 atctccaaaa aaaaaggctc caaaaggagc ctttaattgt atcggtttat cagcttgctt
3601 tcgaggtgaa tttcgaccct ctagagggtc gaccctctag aggctgcagc cgaccccgga
3661 tccggagctt ttagagcaga agtaacactt ccgtacaggc ctagaagtaa aggcaacatc
3721 cactgaggag cagttctttg atttgcacca ccaggagcct caaatttttc aataaattca
3781 cctgactgca cattaggact ttggctttga gcttcccacc tctcagttac ttgctgaata
3841 ctgtctgctt catcaatatt atcataggtg tgcccaaatg atatttgcaa cccttccctg
3901 ttggctactt gtctcaccat tgtaggccta atgggagaca aagtagagta gtaatcttgt
3961 aaagagttat accaattaac aggagcatta attactgtcc aagtagtttc ctctaaaaac
4021 cttgccaaac tgtcccttaa atatctttgg gttcttcttt caagctcctg tgaggtgagc
4081 ctaggaatgt cattttgtat tacacgccaa aaagcttgag aaatggcatt aaaaagtgtt
4141 ggaccccaat gtctggggtc aagatactga acactgtgaa caaaggtttg tactccagga
4201 aataaaatat catagtaatc atctggccta tacaaatcta cagccattcc tggttgttga
4261 tataaaccaa cagtagaaac tttgtgatcc cagtcactaa aaaatctata ccccacttga
4321 gcaacagcgc tcacaccagt cacagtttgc agtaaagctg caaatccagc tatagcagca
4381 ggagccccag atatcacagc ataggcctgt ggagtgaggc ctatagcagc aattgcctca
4441 gaggttgtta ggccttcaac agtagcaaca gatgcaagtt gcacttcaat tgcagcagcg
4501 gcctctccag cagcaatttc agctactgaa aatccagtag cagcagcagc ttcagacaca
4561 gtagcaatta ggtcccccaa cagtgttaaa gcagcaccca tggacctgaa ataaaagaca
4621 aaaagactaa acttaccagt taactttctg gtttttcagt taacctttct ggttttttgc
4681 gtttcccgtc aacagtatct tccccttcac aaaattgcag caaaagctct aaaacaaaca
4741 caaaaaggcg ttgagctgtt tttttacttt cagtccatga cctacgaacc ttaacggagg
4801 cctggcgtga cagccggcgc agcaccatgg cctgaaataa cctctgaaag aggaacttgg
4861 ttaggtacct tctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga
4921 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
4981 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
5041 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc
5101 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
5161 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
5221 ttttgcaaaa agctttgcaa agatggataa agttttaaac agagaggaat ctttgcagct
5281 aatggacctt ctaggtcttg aaaggagtgc ctgggggaat attcctctga tgagaaaggc
5341 atatttaaaa aaatgcaagg agtttcatcc tgataaagga ggagatgaag aaaaaatgaa
5401 gaaaatgaat actctgtaca agaaaatgga agatggagta aaatatgctc atcaacctga
5461 ctttggaggc ttctgggatg caactgaggt atttgcttct tccttaaatc ctggtgttga
5521 tgcaatgtac tgcaaacaat ggcctgagtg tgcaaagaaa atgtctgcta actgcatatg
5581 cttgctgtgc ttactgagga tgaagcatga aaatagaaaa ttatacagga aagatccact
5641 tgtgtgggtt gattgctact gcttcgattg ctttagaatg tggtttggac ttgatctttg
5701 tgaaggaacc ttacttctgt ggtgtgacat aattggacaa actacctaca gagatttaaa
5761 gctctaaggt aaatataaaa tttttaagtg tataatgtgt taaactactg attctaattg
5821 tttgtgtatt ttagattcca acctatggaa ctgatgaatg ggagcagtgg tggaatgcct
5881 ttaatgagga aaacctgttt tgctcagaag aaatgccatc tagtgatgat gaggctactg
5941 ctgactctca acattctact cctccaaaaa agaagagaaa ggtagaagac cccaaggact
6001 ttccttcaga attgctaagt tttttgagtc atgctgtgtt tagtaataga actcttgctt
6061 gctttgctat ttacaccaca aaggaaaaag ctgcactgct atacaagaaa attatggaaa
6121 aatattctgt aacctttata agtaggcata acagttataa tcataacata ctgttttttc
6181 ttactccaca caggcataga gtgtctgcta ttaataacta tgctcaaaaa ttgtgtacct
6241 ttagcttttt aatttgtaaa ggggttaata aggaatattt gatgtatagt gccttgacta
6301 gagatccatt ttctgttatt gaggaaagtt tgccaggtgg gttaaaggag catgatttta
6361 atccagaaga agcagaggaa actaaacaag tgtcctggaa gcttgtaaca gagtatgcaa
6421 tggaaacaaa atgtgatgat gtgttgttat tgcttgggat gtacttggaa tttcagtaca
6481 gttttgaaat gtgtttaaaa tgtattaaaa aagaacagcc cagccactat aagtaccatg
6541 aaaagcatta tgcaaatgct gctatatttg ctgacagcaa aaaccaaaaa accatatgcc
6601 aacaggctgt tgatactgtt ttagctaaaa agcgggttga tagcctacaa ttaactagag
6661 aacaaatgtt aacaaacaga tttaatgatc ttttggatag gatggatata atgtttggtt
6721 ctacaggctc tgctgacata gaagaatgga tggctggagt tgcttggcta cactgtttgt
6781 tgcccaaaat ggattcagtg gtgtatgact ttttaaaatg catggtgtac aacattccta
6841 aaaaaagata ctggctgttt aaaggaccaa ttgatagtgg taaaactaca ttagcagctg
6901 cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt
6961 cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg
7021 tgttggcggg tgtcggggcg cagccatgac ccagtcacgt agcgatagcg gagtgtatac
7081 tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa
7141 ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gctcttccgc ttcctcgctc
7201 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
7261 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
7321 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
7381 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
7441 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
7501 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
7561 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
7621 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
7681 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
7741 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
7801 agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
7861 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
7921 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
7981 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
8041 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
8101 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
8161 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
8221 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
8281 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
8341 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
8401 tcgccagtta atagtttgcg caacgttgtt gccattgctg caggcatcgt ggtgtcacgc
8461 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
8521 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
8581 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
8641 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
8701 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa taccgcgcca
8761 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
8821 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
8881 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
8941 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
9001 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
9061 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
9121 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt
9181 cgtcttcaag aa
//