LOCUS LT727589 4904 bp DNA circular SYN 06-FEB-2017
DEFINITION Vector pATIPMA, complete sequence.
ACCESSION LT727589
VERSION LT727589.1
KEYWORDS .
SOURCE Vector pATIPMA
ORGANISM Vector pATIPMA
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 4904)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..4904
/organism="Vector pATIPMA"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 1774."
/db_xref="taxon:1945247"
misc_feature complement(148..413)
/note="M2"
CDS complement(369..1127)
/transl_table=11
/note="M1"
/protein_id="SJL88922.1"
/translation="MSLLTEVETYVLSIIPSGPLKAEIAQRLEDVFAGKNTDLEVLME
WLKTRPILSPLTKGILGFVFTLTVPSERGLQRRRFVQNALNGNGDPNNMDKAVKLYRK
LKREITFHGAKEISLSYSAGALASCMGLIYNRMGAVTTEVAFGLVCATCEQIADSQHR
SHRQMVTTTNPLIRHENRMVLASTTAKAMEQMAGSSEQAAEAMEVASQARQMVQAMRT
IGTHPSSSAGLKNDLLENLQAYQKRMGVQMQRFK"
misc_feature complement(414..1101)
/note="intron"
misc_feature complement(1102..1127)
/note="M2"
CDS 1332..2522
/transl_table=11
/note="TETs"
/protein_id="SJL88923.1"
/translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASH
YGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAG
RIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLH
APFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTV
FFIMQLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGE
KQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQG
QLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSR
ATST"
rep_origin complement(2895..3295)
/note="pMB1 ori"
CDS complement(3836..4696)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL88924.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
BASE COUNT 1157 a 1311 c 1189 g 1247 t
ORIGIN
1 ttctcatgtt tgacagctta tcatcggagc tgaatgaagc cataccaaac gacgagcgtg
61 acaccacgat gcctgcaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
121 aaaaaaaaaa aaaaaaaaaa aaaaaaaact ccagctctat gctgacaaaa tgaccatcgt
181 cagcatccac agcactctgc tgttcctttc gatattcttc cctcatagac tttggcactc
241 cttccgtaga aggccctcct ttcagtccgt atttaaagcg acggtaaatg catttgaaaa
301 aaagacgatc aagaatccac aatatcaagt gcaagatccc aatgatattt gcggcaatag
361 cgagaggatc acttgaaccg ttgcatctgc acccccattc gtttctgata ggcctgcaaa
421 ttttcaagaa gatcattttt cagaccagca ctggagctag gatgagtccc aatggttctc
481 atcgcttgca ccatttgcct agcctgacta gcaacctcca tggcctctgc tgcttgctca
541 ctcgatccag ccatttgctc catagcctta gctgtagtgc tggctaaaac cattctgttc
601 tcatgtctga ttagtgggtt ggttgttgtc accatttgcc tatgagaccg atgctgggag
661 tcagcaatct gttcacaggt tgcacatacc aggccaaatg ccacttcagt ggtcacagcc
721 cccatcctgt tgtatatgag gcccatacaa ctggcaagtg caccagcaga ataactgagt
781 gagatttctt tggccccatg gaatgttatc tccctcttga gcttcctata cagtttaact
841 gctttgtcca tgttatttgg atccccgttc ccattaaggg cattttggac aaagcgtcta
901 cgctgcagtc ctcgctcact gggcacggtg agcgtgaaca caaatcctaa aatcccctta
961 gtcagaggtg acaggattgg tcttgtcttt agccattcca tgagaacctc aagatcggtg
1021 ttcttccctg caaagacatc ttcaagtctc tgtgcgatct cggctttgag ggggcctgac
1081 gggatgatag agagaacgta cgtttcgacc tcggttagaa gactcatctt tcaatatcta
1141 cctgctttcg cttttttttt tttttttttt tttttttttt tttttttttt tttttttttt
1201 tttttttttt gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
1261 tctagcttcc cgataagctt taatgcggta gtttatcaca gttaaattgc taacgcagtc
1321 aggcaccgtg tatgaaatct aacaatgcgc tcatcgtcat cctcggcacc gtcaccctgg
1381 atgctgtagg cataggcttg gttatgccgg tactgccggg cctcttgcgg gatatcgtcc
1441 attccgacag catcgccagt cactatggcg tgctgctagc gctatatgcg ttgatgcaat
1501 ttctatgcgc acccgttctc ggagcactgt ccgaccgctt tggccgccgc ccagtcctgc
1561 tcgcttcgct acttggagcc actatcgact acgcgatcat ggcgaccaca cccgtcctgt
1621 ggatcctcta cgccggacgc atcgtggccg gcatcaccgg cgccacaggt gcggttgctg
1681 gcgcctatat cgccgacatc accgatgggg aagatcgggc tcgccacttc gggctcatga
1741 gcgcttgttt cggcgtgggt atggtggcag gccccgtggc cgggggactg ttgggcgcca
1801 tctccttgca tgcaccattc cttgcggcgg cggtgctcaa cggcctcaac ctactactgg
1861 gctgcttcct aatgcaggag tcgcataagg gagagcgtcg accgatgccc ttgagagcct
1921 tcaacccagt cagctccttc cggtgggcgc ggggcatgac tatcgtcgcc gcacttatga
1981 ctgtcttctt tatcatgcaa ctcgtaggac aggtgccggc agcgctctgg gtcattttcg
2041 gcgaggaccg ctttcgctgg agcgcgacga tgatcggcct gtcgcttgcg gtattcggaa
2101 tcttgcacgc cctcgctcaa gccttcgtca ctggtcccgc caccaaacgt ttcggcgaga
2161 agcaggccat tatcgccggc atggcggccg acgcgctggg ctacgtcttg ctggcgttcg
2221 cgacgcgagg ctggatggcc ttccccatta tgattcttct cgcttccggc ggcatcggga
2281 tgcccgcgtt gcaggccatg ctgtccaggc aggtagatga cgaccatcag ggacagcttc
2341 aaggatcgct cgcggctctt accagcctaa cttcgatcac tggaccgctg atcgtcacgg
2401 cgatttatgc cgcctcggcg agcacatgga acgggttggc atggattgta ggcgccgccc
2461 tataccttgt ctgcctcccc gcgttgcgtc gcggtgcatg gagccgggcc acctcgacct
2521 gaatggaagc cggcggcacc tcgctaacgg attcaccact ccaagaattg gagccaatca
2581 attcttgcgg agaactgtga atgcgcaaac caacccttgg cagaacatat ccatcgcgtc
2641 cgccatctcc agcagccgca cgcggcgcat ctcgggcagc gttgggtcct ggccacgggt
2701 gcgcatgatc gtgctcctgt cgttgaggac ccggctaggc tggcggggtt gccttactgg
2761 ttagcagaat gaatcaccga tacgcgagcg aacgtgaagc gactgctgct gcaaaacgtc
2821 tgcgacctga gcaacaacat gaatggtctt cggtttccgt gtttcgtaaa gtctggaaac
2881 gcggaagtca gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
2941 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
3001 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
3061 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
3121 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
3181 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
3241 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
3301 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
3361 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
3421 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
3481 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc
3541 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
3601 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
3661 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
3721 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
3781 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
3841 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
3901 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
3961 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
4021 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
4081 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
4141 ttgccattgc tgcaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
4201 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
4261 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
4321 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
4381 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
4441 gcccggcgtc aacacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
4501 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
4561 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
4621 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
4681 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
4741 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
4801 gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa
4861 cctataaaaa taggcgtatc acgaggccct ttcgtcttca agaa
//