LOCUS LT727587 4580 bp DNA circular SYN 06-FEB-2017
DEFINITION Vector pMaSPA1, complete sequence.
ACCESSION LT727587
VERSION LT727587.1
KEYWORDS .
SOURCE Vector pMaSPA1
ORGANISM Vector pMaSPA1
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 4580)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..4580
/organism="Vector pMaSPA1"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 1742."
/db_xref="taxon:1945340"
regulatory 24..105
/note="fdT"
/regulatory_class="terminator"
regulatory 134..215
/note="fdT"
/regulatory_class="terminator"
rep_origin 313..768
/note="f1 ori"
rep_origin complement(772..1145)
/note="pMB1 ori"
CDS complement(1686..2546)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL88919.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
misc_feature complement(2861..3520)
/note="chloramphenicol sensitive"
CDS <3765..>4505
/codon_start=1
/transl_table=11
/note="spaf"
/protein_id="SJL88920.1"
/translation="DQRNGFIQSLKDDPSQSANVLGEAQKLNDSQAPKADAQQNNFNK
DQQSAFYEILNMPNLNEAQRNGFIQSLKDDPSQSTNVLGEAKKLNESQAPKADNNFNK
EQQNAFYEILNMPNLNEEQRNGFIQSLKDDPSQSANLLSEAKKLNESQAPKADNKFNK
EQQNAFYEILHLPNLNEEQRNGFIQSLKDDPSQSANLLAEAKKLNDAQAPKADNKFNK
EQQNAFYEILHLPNLTEEQRNGFIQSLKD"
BASE COUNT 1301 a 1102 c 990 g 1187 t
ORIGIN
1 aattcacctc gaaagcaagc tgataaaccg atacaattaa aggctccttt tggagccttt
61 ttttttggag attttcaacg tgaaaaaatt attattcgca attccaagct aattcacctc
121 gaaagcaagc tgataaaccg atacaattaa aggctccttt tggagccttt ttttttggag
181 attttcaacg tgaaaaaatt attattcgca attccaagct ctgcctcgcg cgtttcggtg
241 atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag
301 cggatgcaga tcacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
361 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
421 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
481 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt
541 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
601 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt
661 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
721 aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt tacaatttga tctgcgctcg
781 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
841 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
901 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
961 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
1021 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
1081 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
1141 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
1201 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
1261 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
1321 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt
1381 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
1441 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
1501 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
1561 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
1621 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
1681 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
1741 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
1801 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
1861 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
1921 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
1981 cgcaacgttg ttgccattgc tgcaggcatc gtggtgtcac gctcgtcgtt tggtatggct
2041 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
2101 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
2161 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
2221 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
2281 agttgctctt gcccggcgtc aacacgggat aataccgcgc cacatagcag aactttaaaa
2341 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
2401 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
2461 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
2521 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcagacag
2581 ttttattgtt catgatgata tatttttatc ttgtgcaatg taacatcaga gattttgaga
2641 cacaacgtgg ctttgttgaa taaatcgaac ttttgctgag ttgactcccc gcgcgcgatg
2701 ggtcgaattt gctttcgaaa aaaaagcccg ctcattaggc gggctaaaaa aaagcccgct
2761 cattaggcgg gctcgaattt ctgccattca tccgcttatt atcacttatt caggcgtagc
2821 aaccaggcgt ttaagggcac caataactgc cttaaaaaaa ttacgccccg ccctgccact
2881 catcgcagta ctgttgtaat tcattaagca ttctgccgac atggaagcca tcacagacgg
2941 catgatgaac ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta taatatttgc
3001 ccatagtgaa aacgggggcg aagaagttgt ccatattcgc cacgtttaaa tcaaaactgg
3061 tgaaactcac ccagggattg gctgagacga aaaacatatt ctcaataaac cctttaggga
3121 aataggccag gttttcaccg taacacgcca catcttgcga atatatgtgt agaaactgcc
3181 ggaaatcgtc gtggtattca ctccagagcg atgaaaacgt ttcagtttgc tcatggaaaa
3241 cggtgtaaca agggtgaaca ctatcccata tcaccagctc accgtctttc attgccatac
3301 gaaattccgg atgagcattc atcaggcggg caagaatgtg aataaaggcc ggataaaact
3361 tgtgcttatt tttctttacg gtctttaaaa aggccgtaat atccagctaa acggtctggt
3421 tataggtaca ttgagcaact gactgaaatg cctcaaaatg ttctttacga tgccattggg
3481 atatatcaac ggtggtatat ccagtgattt ttttctccat tttagcttcc ttagctcctg
3541 aaaatctcga taactcaaaa aatacgcccg gtagtgatct tatttcatta tggtgaaagt
3601 tggaacctct tacgtgccga tcaacgtctc attttcgcca aaagttggcc cagggcttcc
3661 cggtatcaac agggacacca ggatttattt attctgcgaa gtgatcttcc gtcacaggta
3721 tttattcgaa gacgaaaggg catcgcgcgc ggggaattcc cggggatcaa cgcaatggtt
3781 ttatccaaag ccttaaagat gatccaagcc aaagtgctaa cgttttaggt gaagctcaaa
3841 aacttaatga ctctcaagct ccaaaagctg atgcgcaaca aaataacttc aacaaagatc
3901 aacaaagcgc cttctatgaa atcttgaaca tgcctaactt aaacgaagcg caacgtaacg
3961 gcttcattca aagtcttaaa gacgacccaa gccaaagcac taacgtttta ggtgaagcta
4021 aaaaattaaa cgaatctcaa gcaccgaaag ctgataacaa tttcaacaaa gaacaacaaa
4081 atgctttcta tgaaatcttg aatatgccta acttaaacga agaacaacgc aatggtttca
4141 tccaaagctt aaaagatgac ccaagccaaa gtgctaacct attgtcagaa gctaaaaagt
4201 taaatgaatc tcaagcaccg aaagcggata acaaattcaa caaagaacaa caaaatgctt
4261 tctatgaaat cttacattta cctaacttaa acgaagaaca acgcaatggt ttcatccaaa
4321 gcctaaaaga tgacccaagc caaagcgcta accttttagc agaagctaaa aagctaaatg
4381 atgctcaagc accaaaagct gacaacaaat tcaacaaaga acaacaaaat gctttctatg
4441 aaattttaca tttacctaac ttaactgaag aacaacgtaa cggcttcatc caaagcctta
4501 aagacgatcc ggggaattcg agctcggtac ccggggatcc tctagagtcg acctgcagcc
4561 aagcttggtc tagaggtcga
//