LOCUS LT727580 4822 bp DNA circular SYN 06-FEB-2017
DEFINITION Vector pATMIFNG, complete sequence.
ACCESSION LT727580
VERSION LT727580.1
KEYWORDS .
SOURCE Vector pATMIFNG
ORGANISM Vector pATMIFNG
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 4822)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..4822
/organism="Vector pATMIFNG"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 1597."
/db_xref="taxon:1945249"
CDS 86..1276
/transl_table=11
/note="tetracyclin resistance"
/protein_id="SJL88905.1"
/translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASH
YGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAG
RIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLH
APFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTV
FFIMQLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGE
KQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQG
QLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSR
ATST"
rep_origin complement(1647..2049)
/note="pMB1 ori"
misc_feature 2907..2992
/note="5UTR"
CDS 2993..3460
/transl_table=11
/note="mIFNG"
/protein_id="SJL88906.1"
/translation="MNATHCILALQLFLMAVSGCYCHGTVIESLESLNNYFNSSGIDV
EEKSLFLDIWRNWQKDGDMKILQSQIISFYLRLFEVLKDNQAISNNISVIESHLITTF
FSNSKAKKDAFMSIAKFEVNNPQVQRQAFNELIRVVHQLLPESSLRKRKRSRC"
misc_feature 3461..4070
/note="3UTR"
gap 4054..4063
/estimated_length=10
BASE COUNT 1141 a 1266 c 1204 g 1197 t
ORIGIN
1 ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa
61 ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg
121 caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt
181 gcgggatatc gtccattccg acagcatcgc cagtcactat ggcgtgctgc tagcgctata
241 tgcgttgatg caatttctat gcgcacccgt tctcggagca ctgtccgacc gctttggccg
301 ccgcccagtc ctgctcgctt cgctacttgg agccactatc gactacgcga tcatggcgac
361 cacacccgtc ctgtggatcc tctacgccgg acgcatcgtg gccggcatca ccggcgccac
421 aggtgcggtt gctggcgcct atatcgccga catcaccgat ggggaagatc gggctcgcca
481 cttcgggctc atgagcgctt gtttcggcgt gggtatggtg gcaggccccg tggccggggg
541 actgttgggc gccatctcct tgcatgcacc attccttgcg gcggcggtgc tcaacggcct
601 caacctacta ctgggctgct tcctaatgca ggagtcgcat aagggagagc gtcgaccgat
661 gcccttgaga gccttcaacc cagtcagctc cttccggtgg gcgcggggca tgactatcgt
721 cgccgcactt atgactgtct tctttatcat gcaactcgta ggacaggtgc cggcagcgct
781 ctgggtcatt ttcggcgagg accgctttcg ctggagcgcg acgatgatcg gcctgtcgct
841 tgcggtattc ggaatcttgc acgccctcgc tcaagccttc gtcactggtc ccgccaccaa
901 acgtttcggc gagaagcagg ccattatcgc cggcatggcg gccgacgcgc tgggctacgt
961 cttgctggcg ttcgcgacgc gaggctggat ggccttcccc attatgattc ttctcgcttc
1021 cggcggcatc gggatgcccg cgttgcaggc catgctgtcc aggcaggtag atgacgacca
1081 tcagggacag cttcaaggat cgctcgcggc tcttaccagc ctaacttcga tcactggacc
1141 gctgatcgtc acggcgattt atgccgcctc ggcgagcaca tggaacgggt tggcatggat
1201 tgtaggcgcc gccctatacc ttgtctgcct ccccgcgttg cgtcgcggtg catggagccg
1261 ggccacctcg acctgaatgg aagccggcgg cacctcgcta acggattcac cactccaaga
1321 attggagcca atcaattctt gcggagaact gtgaatgcgc aaaccaaccc ttggcagaac
1381 atatccatcg cgtccgccat ctccagcagc cgcacgcggc gcatctcggg cagcgttggg
1441 tcctggccac gggtgcgcat gatcgtgctc ctgtcgttga ggacccggct aggctggcgg
1501 ggttgcctta ctggttagca gaatgaatca ccgatacgcg agcgaacgtg aagcgactgc
1561 tgctgcaaaa cgtctgcgac ctgagcaaca acatgaatgg tcttcggttt ccgtgtttcg
1621 taaagtctgg aaacgcggaa gtcagcgctc ttccgcttcc tcgctcactg actcgctgcg
1681 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
1741 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
1801 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
1861 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
1921 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
1981 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
2041 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
2101 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
2161 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
2221 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt
2281 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
2341 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
2401 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
2461 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
2521 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
2581 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
2641 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
2701 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
2761 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
2821 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
2881 tttgcgcaac gttgttgcca ttgctgcagg gggnnccaag gnngatcctt tggaccctct
2941 gacttgagac agaagttctg ggcttctcct cctgcggcct agctctgaga caatgaacgc
3001 tacacactgc atcttggctt tgcagctctt cctcatggct gtttctggct gttactgcca
3061 cggcacagtc attgaaagcc tagaaagtct gaataactat tttaactcaa gtggcataga
3121 tgtggaagaa aagagtctct tcttggatat ctggaggaac tggcaaaagg atggtgacat
3181 gaaaatcctg cagagccaga ttatctcttt ctacctcaga ctctttgaag tcttgaaaga
3241 caatcaggcc atcagcaaca acataagcgt cattgaatca cacctgatta ctaccttctt
3301 cagcaacagc aaggcgaaaa aggatgcatt catgagtatt gccaagtttg aggtcaacaa
3361 cccacaggtc cagcgccaag cattcaatga gctcatccga gtggtccacc agctgttgcc
3421 ggaatccagc ctcaggaagc ggaaaaggag tcgctgctga ttcggggtgg ggaagagatt
3481 gtcccaataa gaataattct gccagcacta tttgaatttt taaatctaaa cctatttatt
3541 aatatttaaa actatttata tggagaatct attttagatg catcaaccaa agaagtattt
3601 atagtaacaa cttatatgtg ataagagtga attcctatta atatatgtgt tatttataat
3661 ttctgtctcc tcaactattt ctctttgacc aattaattat tctttctgac taattagcca
3721 agactgtgat tgcggggttg tatctggggg tgggggacag ccaagcggct gactgaactc
3781 agattgtagc ttgtaccttt acttcactga ccaataagaa acattcagag ctgcagtgac
3841 cccgggagtg ctgctgatgg gaggagatgt ctacactccg ggccagcgct ttaacagcag
3901 gccagacagc actcgaatgt gtcaggtagt aacaggctgt ccctgaaaga aagcagtgtc
3961 tcaagagact tgacactggt cttccctata cagctgaaaa ctgtgactac acccgaatga
4021 caaataactc gctcatttat agtttatcac tgtnnnnnnn nnnccccctg caggcatcgt
4081 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
4141 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
4201 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
4261 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
4321 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa
4381 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
4441 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
4501 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
4561 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
4621 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
4681 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
4741 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac
4801 gaggcccttt cgtcttcaag aa
//