LOCUS LT727579 3740 bp DNA circular SYN 06-FEB-2017
DEFINITION Vector pDMI,1, complete sequence.
ACCESSION LT727579
VERSION LT727579.1
KEYWORDS .
SOURCE Vector pDMI,1
ORGANISM Vector pDMI,1
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 3740)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..3740
/organism="Vector pDMI,1"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 1594."
/db_xref="taxon:1945283"
CDS 354..1148
/transl_table=11
/note="neomycin"
/protein_id="SJL88903.1"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
CDS complement(1526..2608)
/transl_table=11
/note="lacI"
/protein_id="SJL88904.1"
/translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAE
LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS
GVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSII
FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG
DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT
EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNT
QTASPRALADSLMQLARQVSRLESGQ"
regulatory complement(2609..2686)
/note="lacIq promoter"
/regulatory_class="promoter"
rep_origin complement(2987..3485)
/note="p15A ori"
BASE COUNT 813 a 1035 c 1056 g 836 t
ORIGIN
1 cttcacgctg ccgcaagcac tcagggcgca agggctgcta aaggaagcgg aacacgtaga
61 aagccagtcc gcagaaacgg tgctgacccc ggatgaatgt cagctactgg gctatctgga
121 caagggaaaa cgcaagcgca aagagaaagc aggtagcttg cagtgggctt acatggcgat
181 agctagactg ggcggtttta tggacagcaa gcgaaccgga attgccagct ggggcgccct
241 ctggtaaggt tgggaagccc tgcaaagtaa actggatggc tttcttgccg ccaaggatct
301 gatggcgcag gggatcaaga tctgatcaag agacaggatg aggatcgttt cgcatgattg
361 aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg
421 actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg
481 ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg
541 aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg
601 ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc
661 tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc
721 tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc
781 gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc
841 aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg
901 atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct
961 tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt
1021 tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc
1081 tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt
1141 tcttctgagc gggactctgg ggttcgaaat gaccgaccaa gcgacgccca acctgccatc
1201 acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg
1261 ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc
1321 cgggctcgat cccctcgcga gttggttcag ctgctgcctg aggctggacg acctcgcgga
1381 gttctaccgg cagtgcaaat ccgtcggcat ccaggaaacc agcagcggct atccgcgcat
1441 ccatgccccc gaactgcagg agtggggagg cacgatggcc gctttggtcg acaattcgcg
1501 ctaacttaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
1561 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgcca
1621 gggtggtttt tcttttcacc agtgagacgg gcaacagctg attgcccttc accgcctggc
1681 cctgagagag ttgcagcaag cggtccacgc tggtttgccc cagcaggcga aaatcctgtt
1741 tgatggtggt taacggcggg atataacatg agctgtcttc ggtatcgtcg tatcccacta
1801 ccgagatatc cgcaccaacg cgcagcccgg actcggtaat ggcgcgcatt gcgcccagcg
1861 ccatctgatc gttggcaacc agcatcgcag tgggaacgat gccctcattc agcatttgca
1921 tggtttgttg aaaaccggac atggcactcc agtcgccttc ccgttccgct atcggctgaa
1981 tttgattgcg agtgagatat ttatgccagc cagccagacg cagacgcgcc gagacagaac
2041 ttaatgggcc cgctaacagc gcgatttgct ggtgacccaa tgcgaccaga tgctccacgc
2101 ccagtcgcgt accgtcttca tgggagaaaa taatactgtt gatgggtgtc tggtcagaga
2161 catcaagaaa taacgccgga acattagtgc aggcagcttc cacagcaatg gcatcctggt
2221 catccagcgg atagttaatg atcagcccac tgacgcgttg cgcgagaaga ttgtgcaccg
2281 ccgctttaca ggcttcgacg ccgcttcgtt ctaccatcga caccaccacg ctggcaccca
2341 gttgatcggc gcgagattta atcgccgcga caatttgcga cggcgcgtgc agggccagac
2401 tggaggtggc aacgccaatc agcaacgact gtttgcccgc cagttgttgt gccacgcggt
2461 tgggaatgta attcagctcc gccatcgccg cttccacttt ttcccgcgtt ttcgcagaaa
2521 cgtggctggc ctggttcacc acgcgggaaa cggtctgata agagacaccg gcatactctg
2581 cgacatcgta taacgttact ggtttcacat tcaccaccct gaattgactc tcttccgggc
2641 gctatcatgc cataccgcga aaggttttgc accattcgat ggtgtcaacg taaatgcatg
2701 ccgcttcgcc ttcgcgcgcg aattgtcgac cctgtccctc ctgttcagct actgacgggg
2761 tggtgcgtaa cggcaaaagc accgccggac atcagcgcta gcggagtgta tactggctta
2821 ctatgttggc actgatgagg gtgtcagtga agtgcttcat gtggcaggag aaaaaaggct
2881 gcaccggtgc gtcagcagaa tatgtgatac aggatatatt ccgcttcctc gctcactgac
2941 tcgctacgct cggtcgttcg actgcggcga gcggaaatgg cttacgaacg gggcggagat
3001 ttcctggaag atgccaggaa gatacttaac agggaagtga gagggccgcg gcaaagccgt
3061 ttttccatag gctccgcccc cctgacaagc atcacgaaat ctgacgctca aatcagtggt
3121 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc cctggcggct ccctcgtgcg
3181 ctctcctgtt cctgcctttc ggtttaccgg tgtcattccg ctgttatggc cgcgtttgtc
3241 tcattccacg cctgacactc agttccgggt aggcagttcg ctccaagctg gactgtatgc
3301 acgaaccccc cgttcagtcc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
3361 acccggaaag acatgcaaaa gcaccactgg cagcagccac tggtaattga tttagaggag
3421 ttagtcttga agtcatgcgc cggttaaggc taaactgaaa ggacaagttt tggtgactgc
3481 gctcctccaa gccagttacc tcggttcaaa gagttggtag ctcagagaac cttcgaaaaa
3541 ccgccctgca aggcggtttt ttcgttttca gagcaagaga ttacgcgcag accaaaacga
3601 tctcaagaag atcatcttat taatcagata aaatatttct agatttcagt gcaatttatc
3661 tcttcaaatg tagcacctga agtcagcccc atacgatata agttgttaat tctcatgttt
3721 gacagcttat catcgataag
//