LOCUS LT727573 4199 bp DNA circular SYN 06-FEB-2017
DEFINITION Vector pBRHIFNGf0, complete sequence.
ACCESSION LT727573
VERSION LT727573.1
KEYWORDS .
SOURCE Vector pBRHIFNGf0
ORGANISM Vector pBRHIFNGf0
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 4199)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..4199
/organism="Vector pBRHIFNGf0"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 1274."
/db_xref="taxon:1945264"
CDS <401..784
/codon_start=1
/transl_table=11
/note="hIFNG"
/protein_id="SJL88893.1"
/translation="AGHSDVADNGTLFLGILKNWKEESDRKIMQSQIVSFYFKLFKNF
KDDQSIQKSVETIKEDMNVKFFNSNKKKRDDFEKLTNYSVTDLNVQRKAIHELIQVMA
ELSPAAKTGKRKRSQMLFRGRRASQ"
misc_feature 785..1279
/note="3UTR"
gap 1270..1279
/estimated_length=10
rep_origin complement(2359..2590)
/note="pMB1 ori"
CDS complement(3131..3991)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL88894.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
BASE COUNT 1011 a 1082 c 1028 g 1068 t
ORIGIN
1 ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa
61 ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg
121 caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt
181 gcgggatatc gtccattccg acagcatcgc cagtcactat ggcgtgctgc tagcgctata
241 tgcgttgatg caatttctat gcgcacccgt tctcggagca ctgtccgacc gctttggccg
301 ccgcccagtc ctgctcgctt cgctacttgg agccactatc gactacgcga tcatggcgac
361 cacacccgtc ctgtggatcc cccccccccc cccccccccc gcaggtcatt cagatgtagc
421 ggataatgga actcttttct taggcatttt gaagaattgg aaagaggaga gtgacagaaa
481 aataatgcag agccaaattg tctcctttta cttcaaactt tttaaaaact ttaaagatga
541 ccagagcatc caaaagagtg tggagaccat caaggaagac atgaatgtca agtttttcaa
601 tagcaacaaa aagaaacgag atgacttcga aaagctgact aattattcgg taactgactt
661 gaatgtccaa cgcaaagcaa tacatgaact catccaagtg atggctgaac tgtcgccagc
721 agctaaaaca gggaagcgaa aaaggagtca gatgctgttt cgaggtcgaa gagcatccca
781 gtaatggttg tcctgcctgc aatatttgaa ttttaaatct aaatctattt attaatattt
841 aacattattt atatggggaa tatattttta gactcatcaa tcaaataagt atttataata
901 gcaacttttg tgtaatgaaa atgaatatct attaatatat gtattattta taattcctat
961 atcctgtgac tgtctcactt aatcctttgt tttctgacta attaggcaag gctatgtgat
1021 tacaaggctt tatctcaggg gccaactagg cagccaacct aagcaagatc ccatgggttg
1081 tgtgtttatt tcacttgatg atacaatgaa cacttataag tgaagtgata ctatccagtt
1141 actgccggtt tgaaaatatg cctgcaatct gagccagtgc tttaatggca tgtcagacag
1201 aacttgaatg tgtcaggtga ccctgatgaa aacatagcat ctcaggagat ttcatgcctg
1261 gtgcttccan nnnnnnnnng gggggggggg gggggggggg gatcctctac gccggacgca
1321 tcgtggccgg catcaccggc gccacaggtg cggttgctgg cgcctatatc gccgacatca
1381 ccgatgggga agatcgggct cgccacttcg ggctcatgag cgcttgtttc ggcgtgggta
1441 tggtggcagg ccccgtggcc gggggactgt tgggcgccat ctccttgcat gcaccattcc
1501 ttgcggcggc ggtgctcaac ggcctcaacc tactactggg ctgcttccta atgcaggagt
1561 cgcataaggg agagcgtcga ccgatgccct tgagagcctt caacccagtc agctccttcc
1621 ggtgggcgcg gggcatgact atcgtcgccg cacttatgac tgtcttcttt atcatgcaac
1681 tcgtaggaca ggtgccggca gcgctctggg tcattttcgg cgaggaccgc tttcgctgga
1741 gcgcgacgat gatcggcctg tcgcttgcgg tattcggaat cttgcacgcc ctcgctcaag
1801 ccttcgtcac tggtcccgcc accaaacgtt tcggcgagaa gcaggccatt atcgccggca
1861 tggcggccga cgcgctgggc tacgtcttgc tggcgttcgc gacgcgaggc tggatggcct
1921 tccccattat gattcttctc gcttccggcg gcatcgggat gcccgcgttg caggccatgc
1981 tgtccaggca ggtagatgac gaccatcagg gacagcttca aggatcgctc gcggctctta
2041 ccagcctaac ttcgatcact ggaccgctga tcgtcacggc gatttatgcc gcctcggcga
2101 gcacatggaa cgggttggca tggattgtag gcgccgccct ataccttgtc tgcctccccg
2161 cgttgcgtcg cggtgcatgg agccgggcca cctcgacctg aatggaagcc ggcggcacct
2221 cgctaacgga ttcaccactc caagaattgg agccaatcaa ttcttgcgga gaactgtgaa
2281 tgcgcaaacc aacccttggc agaacatatc catcgcgtcc gccatctcca gcagccgcac
2341 gcggcgcatc tcgggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
2401 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
2461 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
2521 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
2581 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
2641 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
2701 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
2761 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat
2821 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
2881 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
2941 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
3001 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
3061 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
3121 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
3181 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
3241 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
3301 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
3361 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
3421 gtttgcgcaa cgttgttgcc attgctgcag gcatcgtggt gtcacgctcg tcgtttggta
3481 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
3541 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
3601 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
3661 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
3721 gaccgagttg ctcttgcccg gcgtcaacac gggataatac cgcgccacat agcagaactt
3781 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
3841 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
3901 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
3961 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
4021 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
4081 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta
4141 ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt cttcaagaa
//