LOCUS LT727572 3589 bp DNA circular SYN 06-FEB-2017
DEFINITION Vector pPLi342, complete sequence.
ACCESSION LT727572
VERSION LT727572.1
KEYWORDS .
SOURCE Vector pPLi342
ORGANISM Vector pPLi342
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 3589)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..3589
/organism="Vector pPLi342"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 1221."
/db_xref="taxon:1945366"
CDS complement(52..765)
/transl_table=11
/note="lambda cI857"
/protein_id="SJL88891.1"
/translation="MSTKKKPLTQEQLEDARRLKAIYEKKKNELGLSQESVADKMGMG
QSGVGALFNGINALNAYNAALLAKILKVSVEEFSPSIAREIYEMYEAVSMQPSLRSEY
EYPVFSHVQAGMFSPELRTFTKGDAERWVSTTKKASDSAFWLEVEGNSMTAPTGSKPS
FPDGMLILVDPEQAVEPGDFCIARLGGDEFTFKKLIRDSGQVFLQPLNPQYPMIPCNE
SCSVVGKVIASQWPEETFG"
regulatory complement(766..820)
/note="PRM"
/regulatory_class="promoter"
regulatory 800..847
/note="PR"
/regulatory_class="promoter"
rep_origin 1395..1797
/note="pMB1 ori"
CDS complement(2117..2977)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL88892.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
regulatory 3343..3473
/note="PL"
/regulatory_class="promoter"
BASE COUNT 904 a 926 c 844 g 915 t
ORIGIN
1 ttccggatcc cccccccccc cccccccccg ccgaccagaa caccttgccg atcagccaaa
61 cgtctcttca ggccactgac tagcgataac tttccccaca acggaacaac tctcattgca
121 tgggatcatt gggtactgtg ggtttagtgg ttgtaaaaac acctgaccgc tatccctgat
181 cagtttcttg aaggtaaact catcaccccc aagtctggct atgcagaaat cacctggctc
241 aacagcctgc tcagggtcaa cgagaattaa cattccgtca ggaaagcttg gcttggagcc
301 tgttggtgcg gtcatggaat taccttcaac ctcaagccag aatgcagaat cactggcttt
361 tttggttgtg cttacccatc tctccgcatc acctttggta aaggttctaa gctcaggtga
421 gaacatccct gcctgaacat gagaaaaaac agggtactca tactcacttc taagtgacgg
481 ctgcatacta accgcttcat acatctcgta gatttctctg gcgattgaag ggctaaattc
541 ttcaacgcta actttgagaa tttttgcaag caatgcggcg ttataagcat ttaatgcatt
601 gatgccatta aataaagcac caacgcctga ctgccccatc cccatcttgt ctgcgacaga
661 ttcctgggat aagccaagtt catttttctt tttttcataa attgctttaa ggcgacgtgc
721 gtcctcaagc tgctcttgtg ttaatggttt cttttttgtg ctcatacgtt aaatctatca
781 ccgcaaggga taaatatcta acaccgtgcg tgttgactat tttacctctg gcggtgataa
841 tggttgcatg tggaattagc ttggctgcct cgcgcgtttc ggtgatgacg gtgaaaacct
901 ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag
961 acaagcccgt cctctagagg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
1021 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
1081 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
1141 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc
1201 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
1261 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
1321 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
1381 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
1441 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
1501 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
1561 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
1621 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
1681 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
1741 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
1801 tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tggtgcactc
1861 tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg
1921 tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacggct
1981 ctagagggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
2041 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
2101 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
2161 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
2221 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
2281 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
2341 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
2401 ttaatagttt gcgcaacgtt gttgccattg ctgcaggcat cgtggtgtca cgctcgtcgt
2461 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
2521 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
2581 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
2641 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
2701 tgcggcgacc gagttgctct tgcccggcgt caacacggga taataccgcg ccacatagca
2761 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
2821 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
2881 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
2941 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatg
3001 taagcagaca gttttattgt tcatgatgat atatttttat cttgtgcaat gtaacatcag
3061 agattttgag acacaacgtg gctttgttga ataaatcgaa cttttgctga gttgaaggat
3121 cagatcacgc atcttcccga caacgcagac cgttccgtgg caaagcaaaa gttcaaaatc
3181 accaactggt ccacctacaa caaagctctc atcaaccgtg gctccctcac tttctggctg
3241 gatgatgggg cgattcaggc ctggtatgag tcagcaacac cttcttcacg aggcagacct
3301 cagcgccggt gatgccggcc acgatgcgtc cggcgtagag gatctctcac ctaccaaaca
3361 atgcccccct gcaaaaaata aattcatata aaaaacatac agataaccat ctgcggtgat
3421 aaattatctc tggcggtgtt gacataaata ccactggcgg tgatactgag cacatcagca
3481 ggacgcactg accaccatga aggtgacgct cttaaaatta agccctgaag aagggcagca
3541 ttcaaagcag aaggctttgg ggtgtgtgat acgaaacgaa gcattggaa
//