LOCUS LT727571 4385 bp DNA circular SYN 06-FEB-2017
DEFINITION Vector pAT153HIL2, complete sequence.
ACCESSION LT727571
VERSION LT727571.1
KEYWORDS .
SOURCE Vector pAT153HIL2
ORGANISM Vector pAT153HIL2
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 4385)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..4385
/organism="Vector pAT153HIL2"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 1215."
/db_xref="taxon:1945240"
misc_feature complement(400..674)
/note="3' UTR of hIL2"
CDS complement(675..>1076)
/codon_start=1
/transl_table=11
/note="hIL2 mature sequence"
/protein_id="SJL88889.1"
/translation="APTSSSTKKTQLQLEHLLLDLQMILNGINNYKNPKLTRMLTFKF
YMPKKATELKHLQCLEEELKPLEEVLNLAQSKNFHLRPRDLISNINVIVLELKGSETT
FMCEYADETATIVEFLNRWITFCQSIISTLT"
rep_origin complement(2374..2776)
/note="pMB1 ori"
CDS complement(3317..4177)
/transl_table=11
/note="ampicillin"
/protein_id="SJL88890.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
BASE COUNT 1039 a 1135 c 1087 g 1124 t
ORIGIN
1 ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa
61 ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg
121 caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt
181 gcgggatatc gtccattccg acagcatcgc cagtcactat ggcgtgctgc tagcgctata
241 tgcgttgatg caatttctat gcgcacccgt tctcggagca ctgtccgacc gctttggccg
301 ccgcccagtc ctgctcgctt cgctacttgg agccactatc gactacgcga tcatggcgac
361 cacacccgtc ctgtggatcc cccccccccc ccccccccct tatcaaattt attaaatagt
421 tttactaacc aatctacata gatactatat ttaacattca acataataat aaatattttg
481 ggataaataa gtgaaaccat tttagagccc ctagggctta caaaaagaat cataaaagat
541 ccatatttat agttttaaga ttaagaataa tagttacaat aggtagcaaa ccatacattc
601 aacaataaat ataaaattta aatatttaaa taaatagaag gcctgatatg ttttaagtgg
661 gaagcactta attatcaagt cagtgttgag atgatgcttt gacaaaaggt aatccatctg
721 ttcagaaatt ctacaatggt tgctgtctca tcagcatatt cacacatgaa tgttgtttca
781 gatcccttta gttccagaac tattacgttg atattgctga ttaagtccct gggtcttaag
841 tgaaagtttt tgctttgagc taaatttagc acttcctcca gaggtttgag ttcttcttct
901 agacactgaa gatgtttcag ttctgtggcc ttcttgggca tgtaaaactt aaatgtgagc
961 atcctggtga gtttgggatt cttgtaatta ttaattccat tcaaaatcat ctgtaaatcc
1021 agcagtaaat gctccagttg tagctgtgtt ttctttgtag aacttgaagt aggtgcactg
1081 ttgggggggg gggggggggg gggatcctct acgccggacg catcgtggcc ggcatcaccg
1141 gcgccacagg tgcggttgct ggcgcctata tcgccgacat caccgatggg gaagatcggg
1201 ctcgccactt cgggctcatg agcgcttgtt tcggcgtggg tatggtggca ggccccgtgg
1261 ccgggggact gttgggcgcc atctccttgc atgcaccatt ccttgcggcg gcggtgctca
1321 acggcctcaa cctactactg ggctgcttcc taatgcagga gtcgcataag ggagagcgtc
1381 gaccgatgcc cttgagagcc ttcaacccag tcagctcctt ccggtgggcg cggggcatga
1441 ctatcgtcgc cgcacttatg actgtcttct ttatcatgca actcgtagga caggtgccgg
1501 cagcgctctg ggtcattttc ggcgaggacc gctttcgctg gagcgcgacg atgatcggcc
1561 tgtcgcttgc ggtattcgga atcttgcacg ccctcgctca agccttcgtc actggtcccg
1621 ccaccaaacg tttcggcgag aagcaggcca ttatcgccgg catggcggcc gacgcgctgg
1681 gctacgtctt gctggcgttc gcgacgcgag gctggatggc cttccccatt atgattcttc
1741 tcgcttccgg cggcatcggg atgcccgcgt tgcaggccat gctgtccagg caggtagatg
1801 acgaccatca gggacagctt caaggatcgc tcgcggctct taccagccta acttcgatca
1861 ctggaccgct gatcgtcacg gcgatttatg ccgcctcggc gagcacatgg aacgggttgg
1921 catggattgt aggcgccgcc ctataccttg tctgcctccc cgcgttgcgt cgcggtgcat
1981 ggagccgggc cacctcgacc tgaatggaag ccggcggcac ctcgctaacg gattcaccac
2041 tccaagaatt ggagccaatc aattcttgcg gagaactgtg aatgcgcaaa ccaacccttg
2101 gcagaacata tccatcgcgt ccgccatctc cagcagccgc acgcggcgca tctcgggcag
2161 cgttgggtcc tggccacggg tgcgcatgat cgtgctcctg tcgttgagga cccggctagg
2221 ctggcggggt tgccttactg gttagcagaa tgaatcaccg atacgcgagc gaacgtgaag
2281 cgactgctgc tgcaaaacgt ctgcgacctg agcaacaaca tgaatggtct tcggtttccg
2341 tgtttcgtaa agtctggaaa cgcggaagtc agcgctcttc cgcttcctcg ctcactgact
2401 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
2461 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
2521 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
2581 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
2641 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
2701 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
2761 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
2821 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
2881 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
2941 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga
3001 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
3061 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
3121 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
3181 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
3241 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
3301 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
3361 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
3421 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
3481 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
3541 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
3601 ttaatagttt gcgcaacgtt gttgccattg ctgcaggcat cgtggtgtca cgctcgtcgt
3661 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
3721 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
3781 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
3841 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
3901 tgcggcgacc gagttgctct tgcccggcgt caacacggga taataccgcg ccacatagca
3961 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
4021 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
4081 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
4141 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
4201 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
4261 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa
4321 ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtcttc
4381 aagaa
//