LOCUS LT727569 12555 bp DNA circular SYN 06-FEB-2017
DEFINITION Cloning vector pKT240, complete sequence.
ACCESSION LT727569
VERSION LT727569.1
KEYWORDS .
SOURCE Cloning vector pKT240
ORGANISM Cloning vector pKT240
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 12555)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..12555
/organism="Cloning vector pKT240"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 1174."
/db_xref="taxon:1944716"
CDS 69..872
/transl_table=11
/note="StrA"
/note="streptomycin resistance"
/protein_id="SJL88875.1"
/translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASR
RGELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAW
PSMGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPLHDLL
ARVERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLA
LMIANAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
CDS 872..1708
/transl_table=11
/note="StrB"
/note="streptomycin resistance"
/protein_id="SJL88876.1"
/translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
SY"
rep_origin 2353..2748
/note="RSF1010 ori"
CDS complement(2773..3057)
/transl_table=11
/note="mobC"
/protein_id="SJL88877.1"
/translation="MVKGSNKAADRLAKLEEQRARINAEIQRVRAREQQQERKNETRR
KVLVGAMILAKVNSSEWPEDRLMAAMDAYLERDHDRALFGLPPRQKDEPG"
rep_origin 3088..3175
/note="RSF1010 oriT"
CDS 3256..5385
/transl_table=11
/note="mobA"
/protein_id="SJL88878.1"
/translation="MAIYHLTAKTGSRSGGQSARAKADYIQREGKYARDMDEVLHAES
GHMPEFVERPADYWDAADLYERANGRLFKEVEFALPVELTLDQQKALASEFAQHLTGA
ERLPYTLAIHAGGGENPHCHLMISERINDGIERPAAQWFKRYNGKTPEKGGAQKTEAL
KPKAWLEQTREAWADHANRALERAGHDARIDHRTLEAQGIERLPGVHLGPNVVEMEGR
GIRTDRADVALNIDTANAQIIDLQEYREAIDHERNRQSEEIQRHQRVSGADRTAGPEH
GDTGRRSPAGHEPDPAGQRGAGGGVAESPAPDRGGMGGAGQRVAGGSRRGEQRRAERP
ERVAGVALEAMANRDAGFHDAYGGAADRIVALARPDATDNRGRLDLAALGGPMKNDRT
LQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVLQNTPWLKRMNAQGNDVYIR
PAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKNYQAWVKVADAAGGELRGQ
IARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQPWVLLRESKGKTATAGP
ALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALDEYRSEMAGLVKRFGDD
LSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEADYIERTVSKVMGLPSV
QLARAELARAPAPRQRGMDRGGPDFSM"
CDS 4004..4417
/transl_table=11
/note="mobB"
/protein_id="SJL88879.1"
/translation="MNAIDRVKKSRGINELAEQIEPLAQSMATLADEARQVMSQTQQA
SEAQAAEWLKAQRQTGAAWVELAKELREVAAEVSSAAQSARSASRGWHWKLWLTVMLA
SMMPTVVLLIASLLLLDLTPLTTEDGSIWLRLVAR"
CDS 4414..5385
/transl_table=11
/note="repB"
/protein_id="SJL88880.1"
/translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEV
LQNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSP
KNYQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRA
GYQPWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRR
TALDEYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKP
GHEADYIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
CDS 5660..5866
/transl_table=11
/note="rpsF"
/protein_id="SJL88881.1"
/translation="MKDQKDKQTGDLLASPDAVRQARYAERMKAKGMRQRKFWLTDDE
YEALRECLEELRAAQGGGSDPASA"
CDS 5896..6735
/transl_table=11
/note="repA"
/protein_id="SJL88882.1"
/translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKS
MLALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAV
ADGLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVI
GRMEAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSA
EAEEWGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPR
GEA"
CDS 6722..7573
/transl_table=11
/note="repC"
/protein_id="SJL88883.1"
/translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYD
YGDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWE
AVTAECHVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRL
LSEYASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWI
DPGKTGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDI
TRPKAAG"
misc_feature 7519..8574
/note="RSF'"
CDS complement(9106..9966)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL88884.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
CDS complement(10666..11481)
/transl_table=11
/note="Tn903 neomycin resistance"
/protein_id="SJL88885.1"
/translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLY
GKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIP
GKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGL
VDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVG
RVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
BASE COUNT 2816 a 3588 c 3502 g 2640 t
ORIGIN
1 ttcgttaact gcacattcgg gatatttctc tatattcgcg cttcatcaga aaactgaagg
61 aacctccatt gaatcgaact aatatttttt ttggtgaatc gcattctgac tggttgcctg
121 tcagaggcgg agaatctggt gattttgttt ttcgacgtgg tgacgggcat gccttcgcga
181 aaatcgcacc tgcttcccgc cgcggtgagc tcgctggaga gcgtgaccgc ctcatttggc
241 tcaaaggtcg aggtgtggct tgccccgagg tcatcaactg gcaggaggaa caggagggtg
301 catgcttggt gataacggca attccgggag taccggcggc tgatctgtct ggagcggatt
361 tgctcaaagc gtggccgtca atggggcagc aacttggcgc tgttcacagc ctatcggttg
421 atcaatgtcc gtttgagcgc aggctgtcgc gaatgttcgg acgcgccgtt gatgtggtgt
481 cccgcaatgc cgtcaatccc gacttcttac cggacgagga caagagtacg ccgctgcacg
541 atcttttggc tcgtgtcgaa cgagagctac cggtgcggct cgaccaagag cgcaccgata
601 tggttgtttg ccatggtgat ccctgcatgc cgaacttcat ggtggaccct aaaactcttc
661 aatgcacggg tctgatcgac cttgggcggc tcggaacagc agatcgctat gccgatttgg
721 cactcatgat tgctaacgcc gaagagaact gggcagcgcc agatgaagca gagcgcgcct
781 tcgctgtcct attcaatgta ttggggatcg aagcccccga ccgcgaacgc cttgccttct
841 atctgcgatt ggaccctctg acttggggtt gatgttcatg ccgcctgttt ttcctgctca
901 ttggcacgtt tcgcaacctg ttctcattgc ggacaccttt tccagcctcg tttggaaagt
961 ttcattgcca gacgggactc ctgcaatcgt caagggattg aaacctatag aagacattgc
1021 tgatgaactg cgcggggccg actatctggt atggcgcaat gggaggggag cagtccggtt
1081 gctcggtcgt gagaacaatc tgatgttgct cgaatatgcc ggggagcgaa tgctctctca
1141 catcgttgcc gagcacggcg actaccaggc gaccgaaatt gcagcggaac taatggcgaa
1201 gctgtatgcc gcatctgagg aacccctgcc ttctgccctt ctcccgatcc gggatcgctt
1261 tgcagctttg tttcagcggg cgcgcgatga tcaaaacgca ggttgtcaaa ctgactacgt
1321 ccacgcggcg attatagccg atcaaatgat gagcaatgcc tcggaactgc gtgggctaca
1381 tggcgatctg catcatgaaa acatcatgtt ctccagtcgc ggctggctgg tgatagatcc
1441 cgtcggtctg gtcggtgaag tgggctttgg cgccgccaat atgttctacg atccggctga
1501 cagagacgac ctttgtctcg atcctagacg cattgcacag atggcggacg cattctctcg
1561 tgcgctggac gtcgatccgc gtcgcctgct cgaccaggcg tacgcttatg ggtgcctttc
1621 cgcagcttgg aacgcggatg gagaagagga gcaacgcgat ctagctatcg cggccgcgat
1681 caagcaggtg cgacagacgt catactagat atcaagcgac ttctcctatc ccctgggaac
1741 acatcaatct caccggagaa tatcgctggc caaagcctta gcgtaggatt ccgccccttc
1801 ccgcaaacga ccccaaacag gaaacgcagc tgaaacggga agctcaacac ccactgacgc
1861 atgggttgtt caggcagtac ttcatcaacc agcaaggcgg cactttcggc catccgccgc
1921 gccccacagc tcgggcagaa accgcgacgc ttacagctga aagcgaccag gtgctcggcg
1981 tggcaagact cgcagcgaac ccgtagaaag ccatgctcca gccgcccgca ttggagaaat
2041 tcttcaaatt cccgttgcac atagcccggc aattcctttc cctgctctgc cataagcgca
2101 gcgaatgccg ggtaatactc gtcaacgatc tgatagagaa gggtttgctc gggtcggtgg
2161 ctctggtaac gaccagtatc ccgatcccgg ctggccgtcc tggccgccac atgaggcatg
2221 ttccgcgtcc ttgcaatact gtgtttacat acagtctatc gcttagcgga aagttctttt
2281 accctcagcc gaaatgcctg ccgttgctag acattgccag ccagtgcccg tcactcccgt
2341 actaactgtc acgaacccct gcaataactg tcacgccccc ctgcaataac tgtcacgaac
2401 ccctgcaata actgtcacgc ccccaaacct gcaaacccag caggggcggg ggctggcggg
2461 gtgttggaaa aatccatcca tgattatcta agaataatcc actaggcgcg gttatcagcg
2521 cccttgtggg gcgctgctgc ccttgcccaa tatgcccggc cagaggccgg atagctggtc
2581 tattcgctgc gctaggctac acaccgcccc accgctgcgc ggcaggggga aaggcgggca
2641 aagcccgcta aaccccacac caaaccccgc agaaatacgc tggagcgctt ttagccgctt
2701 tagcggcctt tccccctacc cgaagggtgg gggcgcgtgt gcagccccgc agggcctgtc
2761 tcggtcgatc attcagcccg gctcatcctt ctggcgtggc ggcagaccga acaaggcgcg
2821 gtcgtggtcg cgttcaaggt acgcatccat tgccgccatg agccgatcct ccggccactc
2881 gctgctgttc accttggcca aaatcatggc ccccaccagc accttgcgcc ttgtttcgtt
2941 cttgcgctct tgctgctgtt cccttgcccg cacccgctga atttcggcat tgattcgcgc
3001 tcgttgttct tcgagcttgg ccagccgatc cgccgccttg ttgctcccct taaccatctt
3061 gacaccccat tgttaatgtg ctgtctcgta ggctatcatg gaggcacagc ggcggcaatc
3121 ccgaccctac tttgtagggg agggcgcact taccggtttc tcttcgagaa actggcctaa
3181 cggccaccct tcgggcggtg cgctctccga gggccattgc atggagccga aaagcaaaag
3241 caacagcgag gcagcatggc gatttatcac cttacggcga aaaccggcag caggtcgggc
3301 ggccaatcgg ccagggccaa ggccgactac atccagcgcg aaggcaagta tgcccgcgac
3361 atggatgaag tcttgcacgc cgaatccggg cacatgccgg agttcgtcga gcggcccgcc
3421 gactactggg atgctgccga cctgtatgaa cgcgccaatg ggcggctgtt caaggaggtc
3481 gaatttgccc tgccggtcga gctgaccctc gaccagcaga aggcgctggc gtccgagttc
3541 gcccagcacc tgaccggtgc cgagcgcctg ccgtatacgc tggccatcca tgccggtggc
3601 ggcgagaacc cgcactgcca cctgatgatc tccgagcgga tcaatgacgg catcgagcgg
3661 cccgccgctc agtggttcaa gcggtacaac ggcaagaccc cggagaaggg cggggcacag
3721 aagaccgaag cgctcaagcc caaggcatgg cttgagcaga cccgcgaggc atgggccgac
3781 catgccaacc gggcattaga gcgggctggc cacgacgccc gcattgacca cagaacactt
3841 gaggcgcagg gcatcgagcg cctgcccggt gttcacctgg ggccgaacgt ggtggagatg
3901 gaaggccggg gcatccgcac cgaccgggca gacgtggccc tgaacatcga caccgccaac
3961 gcccagatca tcgacttaca ggaataccgg gaggcaatag accatgaacg caatcgacag
4021 agtgaagaaa tccagaggca tcaacgagtt agcggagcag atcgaaccgc tggcccagag
4081 catggcgaca ctggccgacg aagcccggca ggtcatgagc cagacccagc aggccagcga
4141 ggcgcaggcg gcggagtggc tgaaagccca gcgccagaca ggggcggcat gggtggagct
4201 ggccaaagag ttgcgggagg tagccgccga ggtgagcagc gccgcgcaga gcgcccggag
4261 cgcgtcgcgg gggtggcact ggaagctatg gctaaccgtg atgctggctt ccatgatgcc
4321 tacggtggtg ctgctgatcg catcgttgct cttgctcgac ctgacgccac tgacaaccga
4381 ggacggctcg atctggctgc gcttggtggc ccgatgaaga acgacaggac tttgcaggcc
4441 ataggccgac agctcaaggc catgggctgt gagcgcttcg atatcggcgt cagggacgcc
4501 accaccggcc agatgatgaa ccgggaatgg tcagccgccg aagtgctcca gaacacgcca
4561 tggctcaagc ggatgaatgc ccagggcaat gacgtgtata tcaggcccgc cgagcaggag
4621 cggcatggtc tggtgctggt ggacgacctc agcgagtttg acctggatga catgaaagcc
4681 gagggccggg agcctgccct ggtagtggaa accagcccga agaactatca ggcatgggtc
4741 aaggtggccg acgccgcagg cggtgaactt cgggggcaga ttgcccggac gctggccagc
4801 gagtacgacg ccgacccggc cagcgccgac agccgccact atggccgctt ggcgggcttc
4861 accaaccgca aggacaagca caccacccgc gccggttatc agccgtgggt gctgctgcgt
4921 gaatccaagg gcaagaccgc caccgctggc ccggcgctgg tgcagcaggc tggccagcag
4981 atcgagcagg cccagcggca gcaggagaag gcccgcaggc tggccagcct cgaactgccc
5041 gagcggcagc ttagccgcca ccggcgcacg gcgctggacg agtaccgcag cgagatggcc
5101 gggctggtca agcgcttcgg tgatgacctc agcaagtgcg actttatcgc cgcgcagaag
5161 ctggccagcc ggggccgcag tgccgaggaa atcggcaagg ccatggccga ggccagccca
5221 gcgctggcag agcgcaagcc cggccacgaa gcggattaca tcgagcgcac cgtcagcaag
5281 gtcatgggtc tgcccagcgt ccagcttgcg cgggccgagc tggcacgggc accggcaccc
5341 cgccagcgag gcatggacag gggcgggcca gatttcagca tgtagtgctt gcgttggtac
5401 tcacgcctgt tatactatga gtactcacgc acagaagggg gttttatgga atacgaaaaa
5461 agcgcttcag ggtcggtcta cctgatcaaa agtgacaagg gctattggtt gcccggtggc
5521 tttggttata cgtcaaacaa ggccgaggct ggccgctttt cagtcgctga tatggccagc
5581 cttaaccttg acggctgcac cttgtccttg ttccgcgaag acaagccttt cggccccggc
5641 aagtttctcg gtgactgata tgaaagacca aaaggacaag cagaccggcg acctgctggc
5701 cagccctgac gctgtacgcc aagcgcgata tgccgagcgc atgaaggcca aagggatgcg
5761 tcagcgcaag ttctggctga ccgacgacga atacgaggcg ctgcgcgagt gcctggaaga
5821 actcagagcg gcgcagggcg ggggtagtga ccccgccagc gcctaaccac caactgcctg
5881 caaaggaggc aatcaatggc tacccataag cctatcaata ttctggaggc gttcgcagca
5941 gcgccgccac cgctggacta cgttttgccc aacatggtgg ccggtacggt cggggcgctg
6001 gtgtcgcccg gtggtgccgg taaatccatg ctggccctgc aactggccgc acagattgca
6061 ggcgggccgg atctgctgga ggtgggcgaa ctgcccaccg gcccggtgat ctacctgccc
6121 gccgaagacc cgcccaccgc cattcatcac cgcctgcacg cccttggggc gcacctcagc
6181 gccgaggaac ggcaagccgt ggctgacggc ctgctgatcc agccgctgat cggcagcctg
6241 cccaacatca tggccccgga gtggttcgac ggcctcaagc gcgccgccga gggccgccgc
6301 ctgatggtgc tggacacgct gcgccggttc cacatcgagg aagaaaacgc cagcggcccc
6361 atggcccagg tcatcggtcg catggaggcc atcgccgccg ataccgggtg ctctatcgtg
6421 ttcctgcacc atgccagcaa gggcgcggcc atgatgggcg caggcgacca gcagcaggcc
6481 agccggggca gctcggtact ggtcgataac atccgctggc agtcctacct gtcgagcatg
6541 accagcgccg aggccgagga atggggtgtg gacgacgacc agcgccggtt cttcgtccgc
6601 ttcggtgtga gcaaggccaa ctatggcgca ccgttcgctg atcggtggtt caggcggcat
6661 gacggcgggg tgctcaagcc cgccgtgctg gagaggcagc gcaagagcaa gggggtgccc
6721 cgtggtgaag cctaagaaca agcacagcct cagccacgtc cggcacgacc cggcgcactg
6781 tctggccccc ggcctgttcc gtgccctcaa gcggggcgag cgcaagcgca gcaagctgga
6841 cgtgacgtat gactacggcg acggcaagcg gatcgagttc agcggcccgg agccgctggg
6901 cgctgatgat ctgcgcatcc tgcaagggct ggtggccatg gctgggccta atggcctagt
6961 gcttggcccg gaacccaaga ccgaaggcgg acggcagctc cggctgttcc tggaacccaa
7021 gtgggaggcc gtcaccgctg aatgccatgt ggtcaaaggt agctatcggg cgctggcaaa
7081 ggaaatcggg gcagaggtcg atagtggtgg ggcgctcaag cacatacagg actgcatcga
7141 gcgcctttgg aaggtatcca tcatcgccca gaatggccgc aagcggcagg ggtttcggct
7201 gctgtcggag tacgccagcg acgaggcgga cgggcgcctg tacgtggccc tgaacccctt
7261 gatcgcgcag gccgtcatgg gtggcggcca gcatgtgcgc atcagcatgg acgaggtgcg
7321 ggcgctggac agcgaaaccg cccgcctgct gcaccagcgg ctgtgtggct ggatcgaccc
7381 cggcaaaacc ggcaaggctt ccatagatac cttgtgcggc tatgtctggc cgtcagaggc
7441 cagtggttcg accatgcgca agcgccgcca gcgggtgcgc gaggcgttgc cggagctggt
7501 cgcgctgggc tggacggtaa ccgagttcgc ggcgggcaag tacgacatca cccggcccaa
7561 ggcggcaggc tgaccccccc cactctattg taaacaagac atttttatct tttatattca
7621 atggcttatt ttcctgctaa ttggtaatac catgaaaaat accatgctca gaaaaggctt
7681 aacaatattt tgaaaaattg cctactgagc gctgccgcac agctccatag gccgctttcc
7741 tggctttgct tccagatgta tgctcttctg ctcctgcagc taatggatca ccgcaaacag
7801 gttactcgcc tggggattcc ctttcgaccc gagcatccgt atgatactca tgctcgatta
7861 ttattattat agaagccccc atgaataaat cgctcatcat tttcggcatc gtcaacataa
7921 cctcggacag tttctccgat ggaggccggt atctggcgcc agacgcagcc attgcgcagg
7981 cgcgtaagct gatggccgag ggggcagatg tgatcgacct ggtccggcat ccagcaatcc
8041 cgacgccgcg cctgtttcgt ccgacacaga aatcgcgcgt atgcgccggt gctggacgcg
8101 ctcaggcaga tggcattccc gtctcgctcg acagttatca acccgcgacg caagcctatg
8161 ccttgtcgcg tggtgtggcc tatctcaatg atattcgcgg ttttccagac gctgcgttct
8221 atccgcaatt ggcgaaatca tctgccaaac tcgtcgttat gcattcggtg caagacgggc
8281 aggcagatcg gcgcgaggca cccgctggcg acatcatgga tcacattgcg gcgttctttg
8341 acgcgcgcat cgcggcgctg acgggtgccg gtatcaaacg caaccgcctt gtccttgatc
8401 ccggcatggg gttttttctg ggggctgctc ccgaaacctc gctctcggtg ctggcgcggt
8461 tcgatgaatt gcggctgcgc ttcgatttgc cggtgcttct gtctgtttcg cgcaaatcct
8521 ttctgcgcgc gctcacaggc cgtggtccgg gggtgtcggg gccgcgacac tcgcnnnnnc
8581 ggaagaccac tgcccgctgt tcaccttacg ggctgtctga ttcaggttat ttccgatggc
8641 ggccagctga cgcagtaacg gcggtgccag tgtcggcagt tttccggaac gggcaaccgg
8701 ctcccccagg cagacccgcc gcatccatac cgccagttgt ttaccctcac agcgttcaag
8761 taaccgggca tgttcatcat cagtaacccg tattgtgagc atcctctcgc gtttcatcgg
8821 tatcattacc ccatgaacag aaatccccct tacacggagg catcagtgac taaacaggaa
8881 aaaaccgccc ttaacatggc ccgctttatc agaagccaga cattaacgct gctggagaag
8941 ctcaacgaac tggannnngg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
9001 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
9061 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
9121 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
9181 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
9241 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
9301 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
9361 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
9421 tgcaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
9481 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
9541 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
9601 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
9661 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
9721 aacacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
9781 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
9841 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
9901 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
9961 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
10021 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
10081 ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa
10141 taggcgtatc acgaggccct ttcgtcttca agaattttat aaaccgtgga gcgggcaata
10201 ctgagctgat gagcaatttc cgttgcacca gtgcccttct gatgaagcgt cagcacgacg
10261 ttcctgtcca cggtacgcct gcggccaaat ttgattcctt tcagctttgc ttcctgtcgg
10321 ccctcattcg tgcgctctag gatcctctac gccggacgca tcgtggccgg catcaccggc
10381 gctgaggtct gcctcgtgaa gaaggtgttg ctgactcata ccaggcctga atcgccccat
10441 catccagcca gaaagtgagg gagccacggt tgatgagagc tttgttgtag gtggaccagt
10501 tggtgatttt gaacttttgc tttgccacgg aacggtctgc gttgtcggga agatgcgtga
10561 tctgatcctt caactcagca aaagttcgat ttattcaaca aagccgccgt cccgtcaagt
10621 cagcgtaatg ctctgccagt gttacaacca attaaccaat tctgattaga aaaactcatc
10681 gagcatcaaa tgaaactgca atttattcat atcaggatta tcaataccat atttttgaaa
10741 aagccgtttc tgtaatgaag gagaaaactc accgaggcag ttccatagga tggcaagatc
10801 ctggtatcgg tctgcgattc cgactcgtcc aacatcaata caacctatta atttcccctc
10861 gtcaaaaata aggttatcaa gtgagaaatc accatgagtg acgactgaat ccggtgagaa
10921 tggcaaaagc ttatgcattt ctttccagac ttgttcaaca ggccagccat tacgctcgtc
10981 atcaaaatca ctcgcatcaa ccaaaccgtt attcattcgt gattgcgcct gagcgagacg
11041 aaatacgcga tcgctgttaa aaggacaatt acaaacagga atcgaatgca accggcgcag
11101 gaacactgcc agcgcatcaa caatattttc acctgaatca ggatattctt ctaatacctg
11161 gaatgctgtt ttcccgggga tcgcagtggt gagtaaccat gcatcatcag gagtacggat
11221 aaaatgcttg atggtcggaa gaggcataaa ttccgtcagc cagtttagtc tgaccatctc
11281 atctgtaaca tcattggcaa cgctaccttt gccatgtttc agaaacaact ctggcgcatc
11341 gggcttccca tacaatcgat agattgtcgc acctgattgc ccgacattat cgcgagccca
11401 tttataccca tataaatcag catccatgtt ggaatttaat cgcggcctcg agcaagacgt
11461 ttcccgttga atatggctca taacacccct tgtattactg tttatgtaag cagacagttt
11521 tattgttcat gatgatatat ttttatcttg tgcaatgtaa catcagagat tttgagacac
11581 aacgtggctt tgttgaataa atcgaacttt tgctgagttg aaggatcaga tcacgcatct
11641 tcccgacaac gcagaccgtt ccgtggcaaa gcaaaagttc aaaatcacca actggtccac
11701 ctacaacaaa gctctcatca accgtggctc cctcactttc tggctggatg atggggcgat
11761 tcaggcctgg tatgagtcag caacaccttc ttcacgaggc agacctcagc gctaaccgtt
11821 tttatcaggc tctgggaggc agaataaatg atcatatcgt caattattac ctccacgggg
11881 agagcctgag caaactggcc tcaggcattt gagaagcaca cggtcacact gcttccggta
11941 gtcaataaac cggtaaacca gcaatagaca taagcggcta tttaacgacc ctgccctgaa
12001 ccgacgaccg ggtcgaattt gctttcgaat ttctgccatt catccgctta ttatcactta
12061 ttcaggcgta gcaccaggcg tttaagggca ccaataactg ccttaaaaaa attacgcccc
12121 gccctgccac tcatcgcagt actgttgtaa ttcattaagc attctgccga catggaagcc
12181 atcacagacg gcatgatgaa cctgaatcgc cagcggcatc agcaccttgt cgccttgcgt
12241 ataatatttg cccatggtga aaacgggggc gaagaagttg tccatattgg ccacgtttaa
12301 atcaaaactg gtgaaactca cccagggatt ggctgagacg aaaaacatat tctcaataaa
12361 ccctttaggg aaataggcca ggttttcacc gtaacacgcc acatcttgcg aatatatgtg
12421 tagaaactgc cggaaatcgt cgtggtattc actccagagc gatgaaaacg tttcagtttg
12481 ctcatggaaa acggtgtaac aagggtgaac actatcccat atcaccagct caccgtcttt
12541 cattgccata cggaa
//